We call these mechanism based sirtuin inhibitors SirTraps that trap the enzyme via this ADP ribose adduct onlinelibrary.wiley.com/doi/10.1002/... 2/

A #postdoc position in atypical #ubiquitin signalling and protein binder engineering is opening soon in my lab at DTU Bioengineering! Get in touch if you are interested.

Excited to share the peer-reviewed version of our work, now online in @embojournal.org! Big thanks to our reviewers and @hvodermaier.bsky.social for facilitating this process. Looking forward to continuing this fun collaboration with @michaelnadbio.bsky.social! www.embopress.org/doi/full/10....

Also enjoyed reading the News & Views from @maltegersch.bsky.social, which beautifully summarizes the recent advancements in studying MARUbylation as a novel hybrid PTM. www.nature.com/articles/s41...

Check out our new publication, a collaboration with Giovanni Luchetti and Vishva Dixit @ Genentech. We show that the E.coli effector NleL inhibits intestinal epithelial cell extrusion after #inflammasome activation via degrading the kinases ROCK1 and 2!! www.nature.com/articles/s41... Explainer 1/8

🚨Our paper is out! 🥳 Hijacking a bacterial ABC transporter for efficient genetic code expansion. Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social www.nature.com/articles/s41... it all started years ago with a failed experiment 🧵👇 1/9

You’ve heard of ubiquitination, meet deazaguanylation: Doug Wassarman in our lab discovered phage defense pathways have co-opted Q nucleobase biosynthetic enzymes to catalyze a new form of protein conjugation chemistry @science.org www.science.org/doi/10.1126/...

Allosteric activation of a ubiquitin ligase by an internal kinase domain! Congratulations to our team member Thornton Fokkens for this fascinating discovery in a neglected disease area with strong therapeutic need. Many thanks to all our great collaborators! www.sciencedirect.com/science/arti...

How can mitophagy be an effective quality control mechanism if mtDNA mutations reach high enough levels to cause disease? This question led us into a dark path, full of concepts of evolutionary genetics, germline stem cell biology and mito-nuclear compatibility. www.science.org/doi/10.1126/...

Just out in @science.org: Together with the lab of @wilhelmpalm.bsky.social, we used sequential in-vitro/in-vivo CRISPR screens to decipher metabolic adaptations in tumors. We find that acidosis is a dominant factor that shapes energy metabolism and stress resilience. www.science.org/doi/10.1126/...

new preprint: Ubiquitin is a protein modification linked with degradation but known to regulate other functions. Over 100k ubiquitination sites have been discovered and here we (@julianvangerwen.bsky.social + others) try to prioritize those most critical to the cell www.biorxiv.org/content/10.1...

Are you using DIA mass spectrometry in proteomics? We have just put this paper online: www.biorxiv.org/content/10.1... Here, we came up with some Recommendations for Quantitative Data-Independent Acquisition (DIA) Proteomics using Controlled Quantitative Experiments (CQEs) 1/4

A new paper reports direct ubiquitination of a small molecule BRD1732 in cells. BR31732 ubiquitination is E3 ligase-dependent and leads to inhibition of proteasomal degradation. www.nature.com/articles/s41...

For life to emerge on Earth, peptides must first have formed without the aid of enzymes — but how? Reactions of sulfur-containing molecules might have been key go.nature.com/4oX04jC

Delighted to share our work on cellular ubiquitination of drug-like compounds by HUWE1 - a surprising journey! Kudos to all contributors & first authors, Barbara Orth and Pavel Pohl. Sincere thanks to @ireserra.bsky.social ‬ #NatCommun for expertly guiding the winding publishing path. rdcu.be/eDzPk

Fully‐Functionalized Natural Product Probes to Expand the Chemical Tractability of the Human Proteome onlinelibrary.wiley.... --- #proteomics #prot-paper

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

The BUB1 and BUBR1 paralogs scaffold the kinetochore fibrous corona | Science Advances www.science.org/doi/10.1126/... Congratulations @vcmentowski.bsky.social for solving a remarkably challenging molecular puzzle

Covariation MS uncovers a protein that controls cysteine catabolism www.nature.com/articles/s41... Beautiful work from Ed Chouchani!

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - @helmholtz-hiri.bsky.social go.nature.com/4n7zDpG

New paper alert! Stellar work by brilliant lead first author @annaleder.bsky.social together with project leader @masgu.bsky.social reveals a groundbreaking discovery: PDIA6 forms multi-chaperone condensates creating "folding factories" in the ER. www.nature.com/articles/s41...

Excited to share our latest work developing molecular glue degraders of ZBTB11, now out in @natchembio.nature.com www.nature.com/articles/s41...

Excited to share our latest: we engineered the reactivity of a bacterial E1-like enzyme for ATP-driven modification of C termini. Our tool mimics the logic of peptide bond formation in biology for precision modification of proteins in vitro. 🧪https://rdcu.be/ewN7C

This has a neat mechanism of inhibition: www.nature.com/articles/s41...

This week in Science, researchers report an artificial molecular motor that can twist a molecular thread to form a mechanically interlocked molecule. The approach creates opportunities to fabricate mechanically interlocked molecules with precise structural control. Learn more: scim.ag/40FboGP

Thrilled to share our work on the role of Ataxin-3 in lysosome regeneration published in @embojournal.org . Here we show that Atxn3 targets K48-K63 branched ubiquitin chains on damaged lysosomes and mediates restoration of lysosomal function via microautophagy. www.embopress.org/doi/full/10....

Delighted to share our latest work on ABHD11 as a novel deglutarylating enzyme, and its role in T cell metabolism @natchembio.nature.com rdcu.be/ewiYZ. Many congratulations to co-first authors Guine Grice, Eleanor Minogue and @hudsonwilco.bsky.social! Great team effort with Randall Johnson's lab.

Very happy to see our contribution to a better understanding of Hsp70/90 #chaperones online! Congrats and a big thank you to all co-authors, collaborators and reviewers! #proteostasis @poepsel-lab.bsky.social @crc1430.bsky.social @unidue-zmb.bsky.social

These NPG spinoff journals are out of control

Really excited to share the first chapter of my PhD as a preprint titled: Targeted degradation of USP7 in solid cancer cells reveals disparate effects of deubiquitinase inhibition vs. acute protein depletion: www.biorxiv.org/content/10.1.... If that sounds interesting to you, small tweetorial below👇

Regioselective azole alkylation at gram scale and in late-stage settings now in @angewandtechemie.bsky.social, enabled by engineered enzymes. onlinelibrary.wiley.com/doi/10.1002/...

Only two weeks left to apply for a Flash Talk at the 5th Virtual @chembiotalks.bsky.social! If you are a PhD or postdoc and want a chance to present to a global #ChemBio audience, make sure to check out our abstract challenge: chemistry-europe.onlinelibrary.wiley.com/hub/journal/...

Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma: Molecular Cell www.cell.com/molecular-ce...

New paper out from the Mittag lab: www.biorxiv.org/content/10.1... New adventures in understanding structure and function of SPOP, which involve donuts! 🍩 Congratulations to first authors Matt Cuneo, Ömer Güllülü and Mohamed-Raafet Ammar, and all other authors!

The 13th General Meeting of the International Proteolysis Society will be held in Búsios, Brazil Oct 26-30, 2025. Training workshops will be held at the Instituto Oswaldo Cruz Oct 23-23. Register now! Links below. 1/3

New online: TRIMming centrosomal assemblies

Excellent piece by @julieparsonnet.bsky.social on infectious diseases as root causes of a number of chronic human diseases; pausing research on bacterial and viral infections will limit efforts to cure neurodegeneration, autoimmune, metabolic disease as well as cancer substack.com/inbox/post/1...

Integrative proximal-ubiquitomics profiling for deubiquitinase (DUB) substrate discovery applied to USP30: www.cell.com/cell-chemica...

Sharing the next installment of our fun collaboration with @michaelnadbio.bsky.social at the interface of #ubiquitin and #ADP-ribosylation! Building upon our identification of cellular MARUbylation, we now identify reader/writer E3 ligases that extend K11 polyUb! 🧵 www.biorxiv.org/content/10.1...

A new paper from @yitangucla.bsky.social and colleagues reports amide-forming enzymes in the biosynthesis of a cysteine protease inhibitor E-64 and a library of potential cathepsin inhibitors through enzymatic combinatorial biocatalysis www.nature.com/articles/s41...

Great to see this out. A story that spanned continents, cities and budget codes… www.nature.com/articles/s41... Big thanks to all the teams involved! @clausenlab.bsky.social @e3chembio.bsky.social

There are currently high hopes for the development of novel Parkinson's drugs. Malte Gersch & his team have now deciphered the mode of action of these drugs. The findings can help to develop innovative therapeutics for Prakinson's disease & other disorders. www.mpi-dortmund.mpg.de/news/promisi...

Innovative and truly unique work coming from academic:industry:NIH/NIAID collaboration (and a heroic self-immunized blood donor). www.sciencedirect.com/science/arti...

Super happy to share that our work on the specific inhibition of USP30 - a clinical stage Parkinson's drug target - is now online at NSMB www.nature.com/articles/s41... Have a read if you fancy chimeric protein engineering or a framework for DUB ligandability. Huge congratulations to Nafizul & team!

We uncover that the #USP8 rhodanese domain is a #peptide binding module that binds short linear motifs in potential substrates: biologydirect.biomedcentral.com/articles/10....

Excited that our work revealing the structure and function of the monster stress response silencing factor, the E3 ligase SIFI, is now out! Congratulations to the most amazing team of Zhi Yang, Diane Haakonsen, Michael Heider and Sam Witus! www.nature.com/articles/s41...

Super happy to share that our work on the specific inhibition of USP30 - a clinical stage Parkinson's drug target - is now online at NSMB www.nature.com/articles/s41... Have a read if you fancy chimeric protein engineering or a framework for DUB ligandability. Huge congratulations to Nafizul & team!

Thank you again Chuna Choudhary from @choudharylabcpr.bsky.social at CPR in Copenhagen for visiting us last week for an exiting CRC1430 @crc1430.bsky.social seminar and for discussing the systems properties of ubiquitination www.cell.com/cell/fulltex...

Are you into cryo-ET, in-cell discovery, protein translocation and folding or just cool integration of structural biology, proteomics and bioinformatics? Then see our new work: www.biorxiv.org/content/10.1... Thank you to all coauthors for your amazing work!