Beautiful 🤩 I love that there are still mysteries like this waiting to be unraveled.. (also how much does this look like an impressionist portrait of GroEL-GroES??)

I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

WASHINGTON (AP) — Trump uses platform of presidency to promote unproven ties between Tylenol, vaccines and autism without new evidence.

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles. We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography zenodo.org/records/1717...

Single-sequence deep learning delivers crystal-quality models of covalent K-Ras G12 hotspot complexes [new] 3D K-Ras covalent complex prediction boosts covalent drug design.

Part of something that we have been working on for a while-A 3.1 Å map of a flexibly tethered 38 kDa domain. Hopefully preprinted soon along with a few other structures. #CryoEM

Hah, one more story on Tat machinery detected! ❤️‍🔥 Congratulations to the authors, teams of @smlea.bsky.social @pstansfeld.bsky.social and Ben Berks! www.biorxiv.org/content/10.1...

These results are truly impressive! But I do worry that #cryoEM is moving into a phase where progress is made by optimizing parameter settings for black box programs. The devil is often in the details, and I am not sure how this could be implemented in #relion, as stated, without more info.

And now this is the first statement from a named White House official, hours before the proclamation is set to go into effect. Despite saying “to be clear,” her first tweet in this thread contained an FAQ posted yesterday which contains NONE of the information she lists below.

Automated and modular protein binder design with BinderFlow [new] Streamlines protein binder design by providing a modular, automated, and easily monitored pipeline, increasing accessibility and scalability.

Replacing “duration of status”, eg while enrolled in graduate studies no matter how long it takes, with a fixed term. Note that most US biomed grad programs run average time to PHD of 5-6 years, not four.

$100k annually..?? www.nytimes.com/2025/09/19/u...

Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids. Link: www.biorxiv.org/content/10.1...

This is an amazing paper led by the Grinter lab on bacterial protein antibiotics called pyocins and the mechanism by which they inhibit the BAM outer membrane complex. @rhyswg.bsky.social, @knottrna.bsky.social, @debnathghosal.bsky.social, @doylemt1.bsky.social, @fabianmunder.bsky.social

Does anyone have any info as to whether this affects *all* H-1Bs? Or just a subset?

STRUCTURE OF A NITROGENASE-PII SUPERCOMPLEX New work from the oxidoreductase half of the group. www.biorxiv.org/content/10.1...

Cryo-EM structure of the prohibitin complex in open conformation pubmed.ncbi.nlm.nih.gov/40966277/ #cryoem

For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data. Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...). Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å

RFdiffusion3 is here: www.biorxiv.org/content/10.1...

How the twin-arginine translocase (Tat) system manages to transport folded proteins across membranes without any leaks? To answer this fundamental question we solved the first structure of TatB3C3 complex with bound cargo. Please check out new preprint! t.co/962Kj9pt6F

❄️ NEW PRE-PRINT ❄️ Happy to see our latest work online. Here we present a super simple solution to the preferred orientation problem in single particle cryo-EM: the use of ultrasonic excitation during vitrification! Details in the 🧵 below... #cryoEM #structuralbiology

🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching! Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv! www.biorxiv.org/content/10.1...

To test how few images we could get away with, I took progressively smaller subsamples of the Aca2-RNA blob-picked stack and repeated HR-HAIR with the same random seed. Using a 60k stack (<10% of the total), high res features are still easily discernible in the good class (~20k ptcls)

Another successful approach to the same problem as HR-HAIR! 🤩 I wonder if a combo might be worth a try - use one frequency band to get perfectly centered particles using 2DTM, then re-estimate orientations using HR-HAIR with a non-overlapping frequency band?

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem

Just tried your HR-HAIR + local refinement pipeline (with tweaked settings based on the pre-print) on a dataset of a small protein complex which did not result in any decent ab-initio reconstructions. HR-HAIR took 19 hours, but damn, result is insane! Many thanks!

Looks interesting (particularly for in silico affinity maturation) & code available on github: github.com/TencentAI4S/...

Curious to know how does auxiliary subunit Neto2 modulate kainate receptor (KAR) function? Read our latest study describing GluK2-Neto2 complex-captured in both its activated and true apo states. www.nature.com/articles/s41...

🚨🧠🔬 A major breakthrough in molecular neuroscience: I am excited to share a new story from our lab, published in accelerated format today by @nature.com: "Delta-type glutamate receptors are ligand-gated ion channels" Read more here (free article link): rdcu.be/eGIKz

Experimental verification is increasingly the bottleneck in protein design projects. In this paper, we develop a new mini-binder screening pipeline based on the microfluidic sizing technique called flow-induced dispersion analysis (FIDA). doi.org/10.1002/pro....

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...

We finally wrote up the paper for LocScale-2.0. edu.nl/pvhxv Please try it out. LocScale is evolving & we are actively building in new features and improvements. Your feedback will help us shape the next steps. Code: cryotud.github.io/locscale/ Work by @alok-bharadwaj.bsky.social and Reinier.

Nature is wild..

Featuring #AlphaBridge features from @alvarezsalmoral.bsky.social, with @jonasteuwen.bsky.social and team, our new work from @nkinl.bsky.social nl.bsky.social @oncodeinstitute.bsky.social . Have a look at ahmpc.eu for more!

So happy to see these developments! Particularly the structure of the 29 kDa heterodimer of hemoglobin! Well done Oli & co! Of course can’t overlook the importance of sample prep and data collection - credit to Mark Herzik and Mengyu Wu

Woo! My lab's Nature Protocols paper is out today. In a nutshell, the protocol shows how to use our Cryo-ET software to solve high-res protein structures. I don't think any of the other authors are here, but congrats to Hsuan-Fu, Ye, and Wendy for all of their work! www.nature.com/articles/s41...

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles https://www.biorxiv.org/content/10.1101/2025.09.08.674935v1

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

It seems trendy lately to have visualizations of 3D proteins structures generated by AI. These show very ugly artifacts, akin to AI pictures of humans with the wrong number of fingers. It is really not hard to make a visualization of a random protein structure using free software! #AI-slop

Another tour-de-force from David Baker's lab : custom DNA-binding miniproteins to target specific DNA sequences for diverse applications in gene regulation and editing. With David, nothing is impossible !

1/14 Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵 Free article: go.nature.com/4oRUC1q

Today in emails that 100% reaffirm the decision to exit twitter… 🫠

Paper alert🚨 Label your protein of interest and localize it in cryoET 👁️❄️ Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...

Structural bioinformatics is incredibly powerful on its own or when paired with theory or experiment. One of the PDB's superpowers isn’t from one structure, but comparing many to uncover folds, binding sites, and subtle conformational shifts. chemrxiv.org/engage/chemr...

Happy to share the molecular mechanism of activation of kainate-subtype ionotropic glutamate receptor GluK2 in complex with auxiliary subunits Neto2. In both closed/apo and open states, ligand-binding domain (LBD) layer has 2-fold rotational symmetry! www.nature.com/articles/s41...

Erice Structural Biology Course 2026 (29 May–6 Jun): apply! Train in Cryo-EM, Cryo-ET & ML to study macromolecular assemblies in cells. Hands-on + theory for next-gen structural cell biology. 🔗 erice2026.azuleon.org/application.... #CryoEM #CryoET #MachineLearning #StructuralBiology #CellBiology

HPV is the most common STI. Revisiting the infection pathways, we discover that, contrary to the accepted model of trafficking, HPV stays intact and infectious in host cell lysosomes 🤯. Among other discoveries, we show that the intact virus is a cargo of COPI-coated pits!

This is a super useful feature. Makes it easy to e.g use orientations from subtracted particles to reconstruct from original particles, revert back to globally aligned orientations after focused refinement and classification, and much more besides - very flexible and worth exploring.