Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem

Our November issue is available at journals.iucr.org/d/issues/202... On the cover: recent studies demonstrate that the range of samples suitable for cryo-EM single-particle analysis is expanding towards increasingly more native samples. Read the review at shorturl.at/mitjg

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated. mrc.tal.net/vx/appcentre...

New online: New York City’s structural biology mosaic

OpenFold3-preview (OF3p) is out: a sneak peek of our AF3-based structure prediction model. Our aim for OF3 is full AF3-parity for every modality. We now believe we have a clear path towards this goal and are releasing OF3p to enable building in the OF3 ecosystem. More👇

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️ Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

Inhibition mechanism of pancreatic KATP channels by centipede toxins www.biorxiv.org/content/10.1101/2025.10.27.684718v1 #cryoem

A class like no other!! From #AI structure hallucination 🤖 to #CryoEM structure reality 🔬 by @biozentrum.unibas.ch @unibas.ch undergraduate students👩‍🔬👩‍🎓 in just a few weeks! One of their creations is this beautiful flower-shaped tetrameric pore 🌼, a brand new member of the #ProteinCosmos 🧶🧬 🧪

October release of DAQ Score DB of cryo-EM str validation! Now includes 255,199 protein chains from 17,283 EMDB maps. Fig is an example with an entire helix having a residue shift. daqdb.kiharalab.org Easy to use DAQ for structure validation in your paper: em.kiharalab.org

Excited to release BoltzGen which brings SOTA folding performance to binder design! The best part of this project is collaborating with a broad network of leading wetlabs that test BoltzGen at an unprecedented scale, showing success on many novel targets and pushing the model to its limits!

I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.

We are excited to share our latest work on GLIC published in @pnas.org, where we developed a #cryoEM data processing strategy that allowed us to isolate and reconstruct multiple asymmetric states, revealing detailed insights into the channel’s activation mechanism. tinyurl.com/mwtb4rpj

Helicon: Helical parameter determination and 3D reconstruction from one image pubmed.ncbi.nlm.nih.gov/41130582/ #cryoem

Finally out in print! Long time project with @olexandr.bsky.social and the PHENIX group. Refinement of X-ray and CryoEM structures, using Machine Learned Potentials as the back end to help fitting. This fulfills the old dream of doing QM refinement, but very very cheap www.nature.com/articles/s41...

Visualizing influenza A virus assembly by in situ cryo-electron tomography www.nature.com/articles/s41...

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

Wild

Chris Russo and Anastasiia Gusach have developed a new method to prepare #cryoEM samples that avoids protein damage during freezing Read more: www2.mrc-lmb.cam.ac.uk/a-new-method... #LMBResearch

End-to-end protein design in the browser through evedesign. Generate and interactively explore designs in 2D/3D and export them as codon-optimized DNA. The underlying open source framework (released soon) is build to easily add new methods, more on that soon. 🌐 evedesign.bio

**Job Alert** Exciting postdoc position available: theoretical and experimental cryo-EM studies of flexible biomolecules. Competitive salary, collaborative environment at NYSBC and Flatiron Institute. Please share!! and contact: [email protected]

Is anyone else having problems with NCBI tools? I'm trying to use the CDD database, and it seems completely broken for requests that worked fine before - not sure if shutdown related?

This looks pretty cool and unexpected.... www.biorxiv.org/content/10.1...

So excited to see this project finally come together after 2+ years of development! I’m proud of my contributions, but this success belongs to the entire Structura team — such a great example of what collaboration and creativity can achieve.

End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1

Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️ A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria

We (@sobuelow.bsky.social) developed AF-CALVADOS to integrate AlphaFold and CALVADOS to simulate flexible multidomain proteins at scale See preprint for: — Ensembles of >12000 full-length human proteins — Analysis of IDRs in >1500 TFs 📜 doi.org/10.1101/2025... 💾 github.com/KULL-Centre/...

Structures of rotary ATP synthase from Thermus thermophilus during proton powered ATP synthesis | Science Advances www.science.org/doi/10.1126/...

I ❤️ NYC

I’m proud to live in the state with what looks like the highest density of No Kings protests in the country!

Excellent recommendation, thanks @alisterburt.bsky.social! 🙏 🤓

A university that signs the “compact” is one that acknowledges its own inability to compete and succeed based talent and merit. It would signal insecurity and mediocrity to current and future students and faculty. Say no. Recruit the best people, protect their freedom and support their hard work.

Icecream: High-Fidelity Equivariant Cryo-Electron Tomography [new] Equivariant imaging for cryo-ET denoising/recovery via self-supervision & dose-split data.

1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. www.biorxiv.org/content/10.1...

😮😮😮 www.cell.com/cell/fulltex...

Find a No Kings protest Saturday and go! Don't worry that you've never been to a protest before. Don't worry about being dressed wrong or looking out of place. Don't worry that you don't agree about everything -- or even about much -- with lots of people at the protest. /1

🪧 NO KINGS NYC 📍 Meet: 47th & 7th Ave 📍 March: South on 7th Ave, to 14th St 📆 Oct 18th ⏰ 11am 📣 More info at NoKingsNYC.com 📣 Sign up to volunteer bit.ly/4q3mgZU 📣 Sign up to marshal tinyurl.com/NokingsMarshals We work collaboratively, creatively, respectfully, and with all the joy we can muster!

Great lineup here! 👏

Nominations for the June Almeida award for Mid/Senior-Career Women in Cryo-EM are now open! Submit your nomination via this link www.biophysics.org/awards-fundi... by November 14th to recognize an exceptional woman in our community.

Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control. www.science.org/doi/10.1126/...

🚨Our paper is out! 🥳 Hijacking a bacterial ABC transporter for efficient genetic code expansion. Many congrats to everyone involved - a multi-year effort led by @taruniype.bsky.social @maxfottner.bsky.social www.nature.com/articles/s41... it all started years ago with a failed experiment 🧵👇 1/9

Structure and quenching of a bundle-shaped phycobilisome pubmed.ncbi.nlm.nih.gov/41091862/ #cryoem

You may have noticed that AlphaFold3 does not allow covalent bonds between/within polymer chains (protein, DNA, RNA) but allows it for ligands. We tried to go around this limitation by simply treating one of residues/nucleic acids as a ligand.

Oh no! The volume eraser is back! 😳

Super excited about first Shendure/Baker Lab collaboration & preprint on a multiplex sequencing-based strategy for screening de novo proteome editors in mammalian cells. Kudos to the brilliant Chase Suiter (not here) & @greenahn.bsky.social on the work! Preprint here: www.biorxiv.org/content/10.1...

Missed this at the time, super cool. Domes & capsules everywhere it seems..

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

Looks interesting - currently monomer only, but perhaps one could test on complexes/oligomers using the old GS linker trick? Code available on Github.

Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM pubmed.ncbi.nlm.nih.gov/41083086/ #cryoem

Our paper on the first UV-sensitive rhodopsin channel, the Apusomonad rhodopsin, has been published in PNAS! 🎉 This is a new family distinct from canonical ChRs with a novel transport mechanism🦠—paving the way for future multi-wavelength optogenetics! 🔬 www.pnas.org/doi/full/10.... #OpenAccess

Any structural biologists who want some fun? Try building mirror folds. When you’re building them you seriously innately feel like this is “off” but they do exist! When I looked at some of these datasets and deciding which way to turn in Coot it was so offsetting www.biorxiv.org/content/10.1...