Trimbody with rigid AI-designed scaffolds enables atomic-resolution cryo-EM structure determination of small proteins pubmed.ncbi.nlm.nih.gov/41735342/ #cryoem

The giant viruses surprised us at almost every turn of this project, but ultimately led us down a very rewarding path. Happy to share this work is now available online 🧪

We posted a biorxiv preprint on structural bioinformatics, AlphaFold modeling & machine learning on predicting specificity of E3 ligase ring domains for different E2 enzymes. 1/4 Preprint: www.biorxiv.org/content/10.6... Models/data (UbiqCore website): dunbrack.fccc.edu/ubiqcore

Metadynamics meets diffusion-based structure prediction: the same trick that drives enhance sampling of MD trajectories can be adapted and applied as steering terms during generation to sample diverse conformations. Seems obvious in hindsight!

I am excited to share our latest preprint - An evolutionarily divergent herpesvirus with a giant tail. Featuring symmetry breaking and genome annotation from structure using ModelAngelo. doi.org/10.64898/202...

Structure-guided design of a targeted autoantibody degrader for neurologic disease pubmed.ncbi.nlm.nih.gov/41676581/ #cryoem

How could a simple self-replicating system emerge at the origins of life? RNA polymerase ribozymes can replicate RNA, but existing ones are so large that their self-replication seems impossible. Could they be smaller? Excited to share our latest work in @science.org on a new small polymerase. 1/n

AlignPCA-2D: PCA-Reduced Euclidean Vector Alignment for 2D Classification in Cryo-EM https://www.biorxiv.org/content/10.64898/2026.02.11.705027v1

Some cool E3 ligase structures here with calmodulin-mediated oligomerisation. Also interesting collection/processing to deal with preferred orientation. www.biorxiv.org/content/10.6...

Molecular mechanisms of flotillin complexes in organizing membrane microdomains pubmed.ncbi.nlm.nih.gov/41663364/ #cryoem

Structural basis of caveolin-driven membrane bending https://www.biorxiv.org/content/10.64898/2026.02.05.703862v1

Introducing The Structural History of Eukarya (SHE): The first proteome-scale phylogeny constructed entirely from 3D structure. We computed 300 trillion alignments across 1,542 species to map the tree of life. 🧵👇 (1/5)

Protenix v1.0 is out with some very impressive performance numbers (exceeding AF3 performance on protein-protein complexes)

Robust quality assessment of cryo-EM maps, tomograms and micrographs by statistics-based local resolution estimation https://www.biorxiv.org/content/10.64898/2026.02.03.703505v1

Molecular insights into the capsular polysaccharide transporter Wza-Wzc complex pubmed.ncbi.nlm.nih.gov/41644548/ #cryoem

This just in. So honored and grateful...

Huh - a trimeric aquaporin, that is unexpected (although per the paper, apparently predictable by AF3)

BREAKING: Federal judge orders Adrian Conejo Arias and his minor son, L.C.R., released. "The Great Writ and release from detention are GRANTED." "Civics lesson to the government: Administrative warrants issued by the executive branch to itself do not pass probable cause muster."

Judge Biery signed the order with a photo of Liam and two bible verses: The first is "But Jesus said, Suffer little children, and forbid them not, to come unto me: for of such is the kingdom of heaven." The second is simply "Jesus wept." Astonishing thing for a judge to do.

Formation & function of #MembranelessOrganelles! #CryoET structures of #proteasome storage granules inside cells! Read our paper @cp-cell.bsky.social! ❕Publication: doi.org/10.1016/j.ce... ❕Press Release: www.biochem.mpg.de/en/pressroom @uoftmedicine.bsky.social @erc.europa.eu #UPSmeetMet

🚀 CryoSPARC v5.0 BETA is here! We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread! Full changelog: cryosparc.com/updates/v5.0.0

Check out our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3) 🔗 biorxiv.org/content/10.6...

A lot of nice usability & stability improvements here, and some very interesting looking improvements to ab initio! 👀 cryosparc.com/updates/v5.0...

New preprint from the Robertson Lab! We used AF ensemble generation for rigidity filtering with generative design to make cryoEM fiducials, enabling rapid inactive states of four drug targets and a beta2 extracellular fiducial to study GPCR activation! www.biorxiv.org/content/10.6...

Designing Rigid Protein Fiducials to Visualize GPCR Conformational States [new] Rigid Protein Fiducials Visualize GPCR States: CryoEM visualization enabled via protein design/deep learning fiducials. Shows intractable states.

Jane Richardson was born #OTD in 1941 + Developed the Richardson (ribbon) diagram to represent proteins' 3D structure (becoming a standard representation for protein structures) + MacArthur Fellow, 1985 + Elected, Nat'l Academy of Sciences, 1991 + President, Biophysical Society, 2012 #WomenInSTEM

We are running the cryo-ET image processing workshop again this year! Come learn everything tomo: STA, segmentation, heterogeneity analysis from @baradlab.com @williamnwan.bsky.social and others! Apply!

Walz: "To Americans who are watching this, I've got a question for you: What side do you want to be on? The side of an all powerful federal govt that can kill, injury, menace, & kidnap its citizens off the streets? Or on the side of a nurse at the VA hospital who died bearing witness to such govt?"

A break from the news ❄️ 🐶

Watch this video if you want to cry over overwhelming decency rather than over rage about what some are trying to turn our country into.

Efficient and rapid isolation of native AMPA receptor complexes for cryo-EM pubmed.ncbi.nlm.nih.gov/41578975/ #cryoem

You have to read this. Firsthand affidavit from one of the women who was there and recording the video. She talks about how Alex Pretti was directing traffic when she arrived. She watched him be killed in front of her. She's afraid to go home, worried she'll be arrested.

Kare 11 local news just read, in full, this statement from Michael and Susan Pretti, the parents of Alex Pretti. "Please get the truth out about our son."

Alex Pretti was a colleague at the VA. We hired him to recruit for our trial. He became an ICU nurse- I lover working with him. He was a good kind person who lived to help and these fuckers executed him. White. Hot. Rage.

PartiNet is a dynamic adaptive neural network for high-performance particle picking in cryo-electron microscopy www.biorxiv.org/content/10.64898/2026.01.23.700950v1 #cryoem

Love this #cryoEM in this @nature.com publication looking at a previously uncharacterized clade of Cas nucleases. #Glacios 200kV cryo-TEM giving beautiful image contrast to solve the (relatively) small asymmetric protein even in the presence of glycerol. www.nature.com/articles/s41...

New online: When cryo-EM modeling meets structure prediction

Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7) www.biorxiv.org/content/10.6...

This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]

This figure captures how challenging the #cryoEM analysis was, but it doesn't capture the challenges we had to solve in molecular biology, biochemistry, purification, and functional testing. Every step was hard! So glad to see it available.

Internship opportunity! @jackn.bsky.social and I have opened two Summer Intern positions at Genentech to build structural biology agents. If you are a (pre-PhD) #cryoEM or #Xray structural biologist with strong computational skills & motivation, please apply!

Hi fellow membrane protein #StructuralBiology people! Do you use maltoside detergents / model them in your stuctures? Watch out! ⚠️ Most PDB models of LMNG and GDN are wrong ❌, yet continue to be re-used. Spread the word! See more: doi.org/10.1021/acsc... (w. @sebvidalchem.bsky.social ) #cryoEM

A 1.25 A!!! cryo EM structure of rubisco.

We are thrilled to share our new pre-print on the molecular anatomy of nuclear proteasome in human sperm cells! #In-cellcryo-ET + #SPA + #LM! @piotrkolata.bsky.social @a-dsantos.bsky.social, @tomdendooven.bsky.social!! doi.org/10.64898/202...

very unusual - a tRNA regulated anion channel

Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

The vault associates with membranes in situ https://www.biorxiv.org/content/10.64898/2025.12.12.693869v1

The upcoming relion-5.1 will be able to do old-school 3D subtomos, 3D pseudo-subtomos, as well as 2D stacks. It could be that a true windowing operation in real space removes sufficient low-resolution noise from top/bottom to allow better kickstart of refinement.

Structure and organization of AMPA receptor-TARP complexes in the mammalian cerebellum | Science www.science.org/doi/10.1126/...