🚀 We're excited to announce the stable release of CryoSPARC v5! After a successful public beta and months of testing, we’re excited to share this major milestone, made possible by feedback from the amazing #CryoSPARC community. Learn more 👉 cryosparc.com/blog/v5-0-st... #cryoEM #StructuralBiology

🔬 We’ll be at the 6th NY Area #CryoEM Meeting on May 27, presenting recent developments in #CryoSPARC for Homogeneous Ab Initio Refinement and workflows for small proteins. bit.ly/4dYMUze We’re also proud to support the meeting as a sponsor — stop by our poster to learn more!

cryosparc-tools is a standalone Python library that connects to #CryoSPARC and is designed to advance your #cryoEM data processing via scripting. 🚀 Watch this new video for an overview of cryosparc-tools, and explore the resources linked in the description 👉 youtu.be/QY7t67c9bQ8

What is the value of open data for the life sciences? 📈 New report by Frontier Economics shows that EMBL-EBI data resources save users an average of 11 hours/week/person, leading to £11.8bn/year in research productivity gains. s.embl.org/EMBL-EBI-202... #opendata

What an incredible #Cryo-EM SPA mini-course last week, hosted by @royalmicrosoc.bsky.social & ThermoFisher Scientific! 🚀 Huge thanks to all the participants who joined the webinar, and to the experts who shared their insights on #SingleParticle #cryoEM workflows.

Measles is coming back. Until now, we didn't know how human antibodies actually recognize and neutralize the virus. No structure of either measles surface protein bound to a human antibody had ever been reported. We set out to change that.

We will be at the @proterosma.bsky.social IBA Conference in Boston next week! Stop by our poster to chat about the latest developments in #CryoSPARC, including new results from fully automated data processing for #sbdd repeat-target scenarios & v5 features 🔬  bit.ly/49hQoKo #SingleParticle #Cryo-EM

Last week we had our #CryoSPARC Workshop in collaboration with the Hospital for Sick Children (SickKids) in Toronto 🇨🇦 It was a pleasure to meet all the wonderful participants and learn from each other! We’re looking forward to future #cryoEM workshops! 🚀

🔬After learning how to collect #cryoEM data, this final webinar shows how data processing can unlock the highest-resolution insights possible. Join us for Part 3 of the Cryo-EM SPA mini-course with @royalmicrosoc.bsky.social & ThermoFisher Scientific — register now! 🗓️ May 5 👉 bit.ly/4cdEnrr

We are at the CCP-EM Spring Symposium XII in Nottingham! Come check out our talk (Session 6, 11:00) and Poster 12 (Session 1) to stay updated on new developments for #SingleParticle #Cryo-EM Data Processing in #CryoSPARC!

Part 1 was just the beginning 🚀 After the Cryo-EM SPA mini-course kickoff with @royalmicrosoc.bsky.social & ThermoFisher Scientific, don’t miss: 🗓️ Apr 21: Data Collection 🗓️ May 5: Data Processing (with Structura Biotechnology) Register 👉 bit.ly/4cdEnrr #cryoEM #CryoSPARC

High-resolution reconstructions don’t come from “more data” – they come from better data. Join us for Webinar 2 (Data Collection) in our Cryo-EM SPA introductory mini-course, with the Royal Microscopical Society. 🗓️ April 21, 2026 | 6 pm BST ✅ Register once for access to all sessions: ter.li/pksdqb

Book now for the Thermo Fisher Scientific Cryo-EM SPA introductory mini-course (14 April, 21 April & 5 May) - FREE online webinar series featuring lectures, Q&A and interactive, virtual break-out rooms 🙂 🔬 Book now (FREE): https://ow.ly/ApaU50YxIf5

The 1st UCSC Cryo-EM Workshop - an intensive, hands-on program dedicated to Single Particle Analysis (SPA) will take place June 23–26 at the UCSC Biomolecular Cryo-EM Facility in Santa Cruz, California - Registration closes April 10th - Learn More -> zurl.co/FE5a4

1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).

SPA success is won (or lost) before you sit down at the microscope. Join Thermo Fisher Scientific and the Royal Microscopical Society for Webinar 1 of a free, 3-part Cryo-EM SPA introductory mini-course – focused on SPA Sample Preparation. 🗓️ April 14, 2026 | 6 PM BST Register now: ter.li/pksdqb

At the #cshlcourses cryo-EM course, Rich Waldo from @structurabio.bsky.social gave us a tutorial on cryoSPARC and did a fabulous job! I was honored to be used for an Einstein from noise demo.

We filled day 3 with #cryoEM core knowledge covering foundational math, data analysis workflows and reconstruction practicals. Thanks to Fred Sigworth, David Jeruzalmi & Collin McManus (#cryoSPARC practical). Ready for a deeper dive? cryoemprinciples.yale.edu @structurabio.bsky.social

I've written a review on what I think is an extremely exciting direction in cryo-EM: Cryo-EM of endogenous membrane proteins in their native lipid bilayer Open access in Quarterly Reviews of Biophysics: doi.org/10.1017/S003...

The University of Waterloo is seeking internationally renowned scholars, especially those working in #CryoEM, for the prestigious Canada Impact+ Research Chairs program- Chairs receive long-term funding & institutional support - Learn More zurl.co/brhwu

Reminder to register for an upcoming in-person #CryoSPARC workshop in Toronto, delivered by @sickkidsto.bsky.social and the #CryoSPARC team, in partnership with the Canadian #CryoEM Nexus! Deadline fast approaching!

Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!

🚀 CryoSPARC v5.0 BETA is here! We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread! Full changelog: cryosparc.com/updates/v5.0.0

Interested in learning or strengthening skills in #CryoEM single particle analysis? Apply for the CSHL cryo-EM course happening in March! This is a super intense 2 week course covering theory up through data deposition. Apps due Jan 9! Info here: meetings.cshl.edu/courses.aspx...

Exciting collab with Paul Palme, Peter Imming, & Adrian Richter! Guided by @courbongautier.bsky.social's structure of myco ATP synthase bound to a squaramide, they have made SQAs that are less toxic, more potent, more stable than AstraZeneca's SQ31f #MedChem #TB #NTM pubs.acs.org/doi/full/10....

Featured #paper shows mechanism of #malaria infection blocking. Read "Cryo-EM structure of endogenous Pfs230:Pfs48/45 complex with 6 antibodies reveals mechanisms of malaria transmission-blocking" by @utoronto.ca#paper#malariasing our CMCF beamline. https://bit.ly/47TWrVv 👈

🚀 Hiring: Cryo-EM Application Scientist, Marketing! Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems. structura.bio/careers/appl...

Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem

New lab preprint! @zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes 🧵 tinyurl.com/ukny4ptz

On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look? Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner! guide.cryosparc.com/processing-d...

🚀 Introducing fully automated data processing for repeat-target #cryoEM. Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention. Preprint: www.biorxiv.org/content/10.1...

End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1

Helical Reconstruction of Amyloids in cryoSPARC? We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social. Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...

1/14 Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵 Free article: go.nature.com/4oRUC1q

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM. nimgs.zoom.us/webinar/regi...

Exciting work from the Franklin, @ox.ac.uk, and @diamondlightsource.bsky.social has led to a new method for imaging small proteins (<50 kDa) using cryoEM. By using bifunctional, bispecific nanobody scaffolds, the team have successfully solved the smallest protein structure to date (14 kDa).

Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets! A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social Out now in Nature Chemical Biology @natchembio.nature.com www.nature.com/articles/s41...

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone! www.nature.com/articles/s41...

Flexibility can be frustrating when processing a #cryoEM dataset. If your target has a wobbly domain, check out our new case study on FaNaC1 for continuous heterogeneity tips in #CryoSPARC! guide.cryosparc.com/processing-d...

Get hands-on training on #cryofreezing, #DataAcquisition and more at EMBO Practical Course "Cryo electron microscopy and 3D image processing" in New Delhi, IN, 21 Feb–3 Mar 2026 Registration by 15 Sep Abstract submission by 30 Sep meetings.embo.org/event/26-cem... #cryoEM #EMBOCryo3D #EMBOevents 🧪

That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv! biorxiv.org/content/10.110

When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it! guide.cryosparc.com/processing-d...

Link here! guide.cryosparc.com/processing-d...

Working with symmetric or pseudosymmetric particles in #cryoEM? This TRPV5 and CaM case study includes practical considerations for using symmetry relaxation and 3D classification when analyzing a pseudosymmetric particle in #CryoSPARC!

When I arrived to NYC as a postdoc, NYSBDG was my first local meeting, and I was blown way by the world-class quality of the science. It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!

Looking for a deep dive into 2D classification in #cryoEM? These recent guide pages cover practical aspects & parameter choices when running these jobs in #CryoSPARC, along with the theory behind expectation maximization: guide.cryosparc.com/processing-d... and guide.cryosparc.com/expectation-...

1/x New story - the structure of the Vault cap, at 2.3Å! Matching a beautiful preprint from @sjorsscheres.bsky.social, we show that the cap of the vault particle has C13 symmetry, in contrast to the 39-fold symmetry of the body. Study lead by talented postdoc @huanli00.bsky.social from my group! ⬇️