🚀 New and expanded #CryoSPARC Guide pages are here! 📚 We present to you several new and updated pages, featuring new figures and explanations, along with learning materials designed for both new and advanced #cryoEM practitioners. Brief summary of what’s new in the thread!

✉️ The latest #CryoSPARC Newsletter is out! Highlights include: 🔹 Sept 2026 workshop hosted with SickKids 🇨🇦 🔹 New cryosparc-tools video 🔹 New case study on discrete heterogeneity 🔹 Small-protein #cryoEM advances 🔹 Dynamic masking guide (v5) Read more here: mailchi.mp/b8fb173dac5c...

🔬Using #SingleParticle #cryo-EM on plaque-mimicking nanodiscs, researchers determined multiple connexin assembly states and uncovered lipid-protein interactions that may help understand gap junction organization and function. Read more in the latest #CryoSPARC Blog 👉 cryosparc.com/blog/gap-jun...

🇨🇦 The Toronto-based #CryoSPARC Workshop in collaboration with SickKids is back this September! Dive deep into single particle data processing with the experts at Structura. 🗓️ When | Sept 24-25, 2026 📍Where | SickKids Patient Support Centre, Toronto Info and registration: cvent.me/dL1wem #cryoEM

CryoSPARC workshop in Toronto! Beyond consensus refinements: Advanced cryo-EM data processing with CryoSPARC Sept 24-25, 2026 (part of the Canadian Cryo-EM Nexus) Register here: cvent.me/dL1wem Please see details in the link or the image Alt text @structurabio.bsky.social @sickkidsto.bsky.social

☀️ Last week we had the pleasure of contributing to the UCSC #CryoEM Workshop in Santa Cruz with a hands-on #CryoSPARC data processing session. ✨ Thank you to the organizers and everyone who participated. We look forward to many more opportunities to contribute to workshops like this!

🚀 Can automation scale #cryoEM? We recently demonstrated it is possible to make #cryo-EM more routine and scalable testing across 21 challenging #GPCR datasets, a real-world scenario for #SBDD, achieving results equal or better than manual processing. Read more 👉 cryosparc.com/blog/automat...

☀️ Structura will be at the UCSC CryoEM Workshop in Santa Cruz this week, leading a hands-on workshop on #cryoEM #SingleParticleAnalysis with #CryoSPARC. Looking forward to exchanging ideas and strategies, and chatting about all things cryo-EM! See you there!🔬 👉 sites.google.com/view/ucsccry...

In a recent #NatComm study, researchers leveraged high-resolution #cryoEM structures of the κ-opioid receptor bound to difelikefalin to guide the design of a more selective compound with reduced side-effect potential. 👉 cryosparc.com/blog/kor-sbdd #StructuralBiology #SBDD #CryoSPARC

🔍 Sample heterogeneity is both a challenge and an opportunity in #cryoEM data processing. In our latest #CryoSPARC case study we explore EMPIAR-10927, a dataset with acetogenin-bound mitochondrial Complex I, that reveals many more complexes according to the data processing strategy 👉 bit.ly/4uihh8V

🦠 How does the human immune system defeat measles? #cryoEM and #CryoSPARC reveal the answer. 🔬 A recent study from @lji.org provides new insights into how the human immune system recognizes and neutralizes measles virus. Read more in the latest #CryoSPARC Blog 👉 cryosparc.com/blog/measles

For years, the #cryoEM community viewed ~50 kDa as the practical lower size limit for SPA. This was challenged by Kim et al., who introduced the HR-HAIR method and demonstrated structure determination of proteins below 30 kDa using #CryoSPARC. Read more 👉 bit.ly/3RHMxAt

🚀 We're excited to announce the stable release of CryoSPARC v5! After a successful public beta and months of testing, we’re excited to share this major milestone, made possible by feedback from the amazing #CryoSPARC community. Learn more 👉 cryosparc.com/blog/v5-0-st... #cryoEM #StructuralBiology

🔬 We’ll be at the 6th NY Area #CryoEM Meeting on May 27, presenting recent developments in #CryoSPARC for Homogeneous Ab Initio Refinement and workflows for small proteins. bit.ly/4dYMUze We’re also proud to support the meeting as a sponsor — stop by our poster to learn more!

cryosparc-tools is a standalone Python library that connects to #CryoSPARC and is designed to advance your #cryoEM data processing via scripting. 🚀 Watch this new video for an overview of cryosparc-tools, and explore the resources linked in the description 👉 youtu.be/QY7t67c9bQ8

What is the value of open data for the life sciences? 📈 New report by Frontier Economics shows that EMBL-EBI data resources save users an average of 11 hours/week/person, leading to £11.8bn/year in research productivity gains. s.embl.org/EMBL-EBI-202... #opendata

What an incredible #Cryo-EM SPA mini-course last week, hosted by @royalmicrosoc.bsky.social & ThermoFisher Scientific! 🚀 Huge thanks to all the participants who joined the webinar, and to the experts who shared their insights on #SingleParticle #cryoEM workflows.

Measles is coming back. Until now, we didn't know how human antibodies actually recognize and neutralize the virus. No structure of either measles surface protein bound to a human antibody had ever been reported. We set out to change that.

We will be at the @proterosma.bsky.social IBA Conference in Boston next week! Stop by our poster to chat about the latest developments in #CryoSPARC, including new results from fully automated data processing for #sbdd repeat-target scenarios & v5 features 🔬  bit.ly/49hQoKo #SingleParticle #Cryo-EM

Last week we had our #CryoSPARC Workshop in collaboration with the Hospital for Sick Children (SickKids) in Toronto 🇨🇦 It was a pleasure to meet all the wonderful participants and learn from each other! We’re looking forward to future #cryoEM workshops! 🚀

🔬After learning how to collect #cryoEM data, this final webinar shows how data processing can unlock the highest-resolution insights possible. Join us for Part 3 of the Cryo-EM SPA mini-course with @royalmicrosoc.bsky.social & ThermoFisher Scientific — register now! 🗓️ May 5 👉 bit.ly/4cdEnrr

We are at the CCP-EM Spring Symposium XII in Nottingham! Come check out our talk (Session 6, 11:00) and Poster 12 (Session 1) to stay updated on new developments for #SingleParticle #Cryo-EM Data Processing in #CryoSPARC!

Part 1 was just the beginning 🚀 After the Cryo-EM SPA mini-course kickoff with @royalmicrosoc.bsky.social & ThermoFisher Scientific, don’t miss: 🗓️ Apr 21: Data Collection 🗓️ May 5: Data Processing (with Structura Biotechnology) Register 👉 bit.ly/4cdEnrr #cryoEM #CryoSPARC

High-resolution reconstructions don’t come from “more data” – they come from better data. Join us for Webinar 2 (Data Collection) in our Cryo-EM SPA introductory mini-course, with the Royal Microscopical Society. 🗓️ April 21, 2026 | 6 pm BST ✅ Register once for access to all sessions: ter.li/pksdqb

Book now for the Thermo Fisher Scientific Cryo-EM SPA introductory mini-course (14 April, 21 April & 5 May) - FREE online webinar series featuring lectures, Q&A and interactive, virtual break-out rooms 🙂 🔬 Book now (FREE): https://ow.ly/ApaU50YxIf5

The 1st UCSC Cryo-EM Workshop - an intensive, hands-on program dedicated to Single Particle Analysis (SPA) will take place June 23–26 at the UCSC Biomolecular Cryo-EM Facility in Santa Cruz, California - Registration closes April 10th - Learn More -> zurl.co/FE5a4

1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).

SPA success is won (or lost) before you sit down at the microscope. Join Thermo Fisher Scientific and the Royal Microscopical Society for Webinar 1 of a free, 3-part Cryo-EM SPA introductory mini-course – focused on SPA Sample Preparation. 🗓️ April 14, 2026 | 6 PM BST Register now: ter.li/pksdqb

At the #cshlcourses cryo-EM course, Rich Waldo from @structurabio.bsky.social gave us a tutorial on cryoSPARC and did a fabulous job! I was honored to be used for an Einstein from noise demo.

We filled day 3 with #cryoEM core knowledge covering foundational math, data analysis workflows and reconstruction practicals. Thanks to Fred Sigworth, David Jeruzalmi & Collin McManus (#cryoSPARC practical). Ready for a deeper dive? cryoemprinciples.yale.edu @structurabio.bsky.social

I've written a review on what I think is an extremely exciting direction in cryo-EM: Cryo-EM of endogenous membrane proteins in their native lipid bilayer Open access in Quarterly Reviews of Biophysics: doi.org/10.1017/S003...

The University of Waterloo is seeking internationally renowned scholars, especially those working in #CryoEM, for the prestigious Canada Impact+ Research Chairs program- Chairs receive long-term funding & institutional support - Learn More zurl.co/brhwu

Reminder to register for an upcoming in-person #CryoSPARC workshop in Toronto, delivered by @sickkidsto.bsky.social and the #CryoSPARC team, in partnership with the Canadian #CryoEM Nexus! Deadline fast approaching!

Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!

🚀 CryoSPARC v5.0 BETA is here! We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread! Full changelog: cryosparc.com/updates/v5.0.0

Interested in learning or strengthening skills in #CryoEM single particle analysis? Apply for the CSHL cryo-EM course happening in March! This is a super intense 2 week course covering theory up through data deposition. Apps due Jan 9! Info here: meetings.cshl.edu/courses.aspx...

Exciting collab with Paul Palme, Peter Imming, & Adrian Richter! Guided by @courbongautier.bsky.social's structure of myco ATP synthase bound to a squaramide, they have made SQAs that are less toxic, more potent, more stable than AstraZeneca's SQ31f #MedChem #TB #NTM pubs.acs.org/doi/full/10....

Featured #paper shows mechanism of #malaria infection blocking. Read "Cryo-EM structure of endogenous Pfs230:Pfs48/45 complex with 6 antibodies reveals mechanisms of malaria transmission-blocking" by @utoronto.ca#paper#malariasing our CMCF beamline. https://bit.ly/47TWrVv 👈

🚀 Hiring: Cryo-EM Application Scientist, Marketing! Develop and deliver high-impact scientific marketing content, illustrate the value of new #CryoSPARC features and #cryoEM advances, and help scientists understand how our tools can help solve their problems. structura.bio/careers/appl...

Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem

New lab preprint! @zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes 🧵 tinyurl.com/ukny4ptz

On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look? Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner! guide.cryosparc.com/processing-d...

🚀 Introducing fully automated data processing for repeat-target #cryoEM. Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention. Preprint: www.biorxiv.org/content/10.1...

End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1

Helical Reconstruction of Amyloids in cryoSPARC? We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social. Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...

1/14 Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵 Free article: go.nature.com/4oRUC1q

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

🧪 Skip the trial and error for structure determination of soluble proteins. Join the #webinar 10/8 for a gene-to-structure case study of CYP3A4, a 57 kDa cytochrome P450 monooxygenase, solved to 3.1 Å by #cryoEM. nimgs.zoom.us/webinar/regi...