Drawing of a tuberculosis infection or a watermelon? You decide. (from Robert Koch's illustrations in 1882)

The outer membrane of Gram-negative bacteria blocks many antibiotics. Our latest work reveals that L-type pyocins bypass this barrier by inactivating the BAM complex, killing Pseudomonas aeruginosa without entering the cell, providing a new blueprint for beating antibiotic resistance.

Another successful approach to the same problem as HR-HAIR! 🤩 I wonder if a combo might be worth a try - use one frequency band to get perfectly centered particles using 2DTM, then re-estimate orientations using HR-HAIR with a non-overlapping frequency band?

🚨🧠🔬 A major breakthrough in molecular neuroscience: I am excited to share a new story from our lab, published in accelerated format today by @nature.com: "Delta-type glutamate receptors are ligand-gated ion channels" Read more here (free article link): rdcu.be/eGIKz

Three Associate Professor (or Professor) positions available at Oxford Biology. Come be my colleague! www.biology.ox.ac.uk/article/we-a...

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Advanced #cryoEM workflows are easy in #CryoSPARC: use the Low-Level Results Interface to combine upstream extracted particles with downstream pose and CTF information, use a volume result from an intermediate iteration of a refinement to create a mask, and more! guide.cryosparc.com/application-...

I often barely believe it. Recently we've also been having a lot of luck with single digit nanomolar affinities. This is affinity range distribution from our latest campaign, 42 screened, 22 binders, almost all low nanomolar, some picomolar.

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

Very honored to receive the Royal Society Research Culture Award - this represents the work of a great team who've worked non-stop since we launched bioRxiv in 2013 royalsociety.org/news/2025/08... www.cshl.edu/inglis-and-s...

If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...

We are hiring a Postdoc in membrane protein cryo-EM at the University of Georgia. Looking for candidates with expertise in membrane protein purification and biochemistry. Fully funded for up to 5 years. Join us in Athens, GA. Apply: [email protected]

Structural basis for the dynamic regulation of mTORC1 by amino acids pubmed.ncbi.nlm.nih.gov/40836086/ #cryoem

📄 New paper: 3DEM Structure Map Validation Recommendations Two new metrics assess model-to-map fitness in cryo-EM: 🔹 Q_relative_all → compares avg Q-score across PDB/EMDB 🔹 Q_relative_resolution → compares within similar resolution #3DEM #CryoEM #wwPDB

My longest ever running project, all the stars finally aligned after 13 years! Thank you to Eric Reynolds group for sharing the journey. #bio21-institute, #university-of-melbourne. @sepidehv.bsky.social @craigmorton.bsky.social @debnathghosal.bsky.social www.nature.com/articles/s41...

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

It is my pleasure to announce the @EMBO course on “In situ structural biology by Cryo-FIB and Cryo-ET” taking place @MPI_Biochem from 8.-14. April 2024. If you are interested in learning about cryo-ET and cryo-FIB including lift-out, apply by December 8th! meetings.embo.org/event/24-in-...

Di-Gembodies (dimerized nanobodies) for improved and duplexed CryoEM of small and challenging targets! A collaboration of @cmd.ox.ac.uk, STRUBI, @diamondlightsource.bsky.social, @rosfrankinst.bsky.social Out now in Nature Chemical Biology @natchembio.nature.com www.nature.com/articles/s41...

The Department of Biochemistry @vanderbilt.edu is hiring for an Assistant Professor position! Apply and come be my colleague! apply.interfolio.com/171767

EMC in a complex with a membrane protein dislocase! 🤩 Exciting twist!

1/3 Electricity from air! In the top journal Nano Energy today, we report the first fuel cell that makes power from air alone. We achieved this by using the enzyme Huc to oxidize ambient hydrogen. This results in a continuous low-level power from air and higher outputs with industrial waste gases.

Cryo-EM led analysis of open and closed conformations of Chagas vaccine candidate TcPOP pubmed.ncbi.nlm.nih.gov/40764299/ #cryoem

very cool stuff and interesting note re the importance of linker length in isolation of the complex

🚨 new paper alert 🚨 Here, we present a method for designing protein nanoparticles with two unique faces that can bring together distinct biological targets at controlled distances! This project was led by Dr. Sanela Rankovic is out now in Nature Materials: www.nature.com/articles/s41...

Cryo-EM of fertilisation complex Pfs230-Pfs48/45 from malaria parasites! 💕💗 🦟 Transgenic parasites without Pfs230 D13D14 do not transmit 🦙 💉Nanobodies + mRNA induced antibodies reduce transmission New in @Science.org www.science.org/doi/10.1126/... #ScienceResearch #Malaria #WEHI

Exciting Aug 1️⃣ for two incredible people- Gregor Weiss and Jingwei Xu! Both are starting their independent labs 🔬🧪 TODAY 🥳 I am incredibly thankful 🙏 for their contributions to the lab and that I could be part of their journey -Martin @xujwet.bsky.social @weiss-lab.bsky.social It was a wild ride👇

Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out: www.sciencedirect.com/science/arti...

a compendium of vampires and pirates (and their armories). actually more of an encyclopedia... by @coralietesseur.bsky.social (drawings made by hand!⭐), @ysantin.bsky.social & @lalouxlab.bsky.social read! > www.sciencedirect.com/science/arti... #MicroSky

Generative Design of High-Affinity Peptides Using BindCraft www.biorxiv.org/content/10.1...

our paper is online! rdcu.be/exXBX . Who would have thought that some #giantviruses not only have #histones, but even histone variants that make weird #nucleosomes (pdb 9CVT). Fantastic work from Ale Villalta and Chelsea Toner, with Hugo Bisio @molbiolgv.bsky.social and Chantal Abergel

Tenure-track faculty search of the UCSF Biochemistry & Biophysics Department is open for applications! We are looking for creative, innovative scientists asking fundamental questions in any area of biology. Join our vibrant, collaborative, and supportive community! aprecruit.ucsf.edu/JPF05702

Our article showing energy production from air alone is in PNAS today. This paper provides an ultimate biochemical proof that microbes can survive simply by 'making' energy from air (making two ATP per molecule atmospheric H2 consumed) and has biotech applications. www.pnas.org/doi/10.1073/...

Check out this weird protein we found in a Loki Asgard archaea.🧬🧶Only found relatives in other Lokis. 🤔 www.biorxiv.org/content/10.1...

Code and tutorials 💻: github.com/macwiatrak/B... Blog ✍️: macwiatrak.github.io/posts/2025/i... Pretrained weights 🤖: huggingface.co/macwiatrak 🧵 2/n

💥 Excited to introduce Bacformer 🦠 - the first foundation model for bacterial genomics. Bacformer represents genomes as sequences of ordered proteins, learning the “grammar” of how genes are arranged, interact and evolve. Preprint 📝: biorxiv.org/content/10.1... 🧵 1/n

Folddisco webserver result view update: - Added description texts for AFDB - Integrated TaxoView taxonomy visualization & filter by @sunjaelee.bsky.social - Inter-residue distance clustering by DBSCAN to explore motif diversity. 🌐 search.foldseek.com/folddisco 📄 www.biorxiv.org/content/10.1...

This study of imidazole glycerol phosphate dehydratase suggests that cryo-EM can be useful in pinpointing the mode of binding small molecules of low mass and mapping protein-ligand interactions #CryoEM #Tuberculosis #HistidineBiosynthesisPathway doi.org/10.1107/S205...

Delighted to share the final version of Richard Zhou's study on how the CD163 scavenger receptor cleans up after toxic haemoglobin spills out from our blood cells. An amazing grabber mechanism! rdcu.be/ewPA2

Happy to share our latest work on the structure and assembly of holocentric kinetochores! Huge thanks to Ines for a very fruitful collaboration, Claudio for all the support, and congratulations to Christine and all co-authors! www.biorxiv.org/content/10.1...

Conduction pathway for potassium through the E. coli pump KdpFABC pubmed.ncbi.nlm.nih.gov/40654642/ #cryoem

Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...

Returned from a wonderful symposium in honour of the 50th anniversary of Richard Henderson's and Nigel Unwin's 1975 paper on the structure of bacteriorhodopsin - the paper that started the field of membrane protein structural biology. Also fulfilled my dream of ringing the LMB seminar bell.

Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social. www.science.org/doi/10.1126/...

New in @science.org We built a safe, atomic-resolution version of the Powassan virus—a deadly tick-borne #flavivirus with no vaccine. ✅ First structure ever ✅ Safe to handle (BSL-2) ✅ Platform for vaccines & antivirals Let me show you 🧵 (1/5) Link: www.science.org/doi/10.1126/...

🚀 My first Cryo-EM structures are OUT on the PDB! 🎉Super excited about this milestone as PhD, it’s been a journey, and I’m grateful to finally have these as my first Cryo-EM structures! 🙌 🔁Thank you for sharing!❤️ Read @daaninthelab.bsky.social et. al.: www.science.org/doi/10.1126/... ⬇️ 2nd video

A novel "bio-tag" for cryo-EM studies based on the small, electron-dense protein Csp1 pubmed.ncbi.nlm.nih.gov/40620219/ #cryoem

Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.

More arguments for choosing society journals, where any profits are ploughed back into science! For structural biology, please consider @actacrystd.iucr.org, @actacrystf.iucr.org and @iucrj.iucr.org!

Also a couple of back-to-back @natcomms.nature.com papers with @chelseabrown.bsky.social on Mycobacterial enzymes in collaboration with the Mancia lab et al.: doi.org/10.1038/s414... doi.org/10.1038/s414...

the really key number to remember is that biorxiv serves the entire global science community, posting thousands of papers per month for millions of readers, and it's entire running costs ( a few $M/year) are less than some single labs....