Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1... Soon at @spirochrome.com Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

Be ready for an exciting meeting!

🚨 New EMBO Practical Course! Ultrastructure Expansion Microscopy: From Cells to Tissue 🔬 📅 20–24 Apr 2026 | 📍 EMBL Heidelberg Co-organised with @banterlegroup.bsky.social @gautamdey.bsky.social 💡 Register your interest to get notified when registration opens: 🔗 www.embl.org/about/info/c...

💼 PostDoc position (2 years) on Trypanosoma brucei | Bordeaux, France | Lab MFP with Dr. Frédéric Bringaud @univbordeaux.bsky.social @cnrsaquitaine.bsky.social 🔬 Glycerol and differentiation in Trypanosoma brucei 🗓️ Deadline: September 15. Start: by November 2025 labex-parafrap.fr/fr/emplois/4...

On Friday, Daniel left us after more than 4 happy years in the lab @pasteur.fr and a great paper published today in @jcellsci.bsky.social as well as other contributions! Many thanks for everything! journals.biologists.com/jcs/article-...

#Trypanosoma, fixed, expanded, and imaged with #STED, stained for tubulin. Scale bar refers to expanded specimen, original structures are 3-3x smaller. Image courtesy: @mbonhivers.bsky.social, MFP, CNRS, Univ. Bordeaux, France #fluorescencefriday #ExM #STEDmicroscopy

🚨 New preprint! Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM www.biorxiv.org/content/10.1...

Honoured to have been introduced today by former lab member @ebertiaux.bsky.social at the annual Microbiology day of New Aquitaine. My first talk in a (former) church!

#Phosphofructokinase (PFK) is considered an irreversible glycolytic #enzyme. This study shows that #Trypanosomes are the only known organism to use PFK in the gluconeogenic direction under normal conditions, likely due to its compartmentalization within glycosomes @plosbiology.org 🧪 plos.io/43r84Qc

Huge congratulations to @marinelap.bsky.social for receiving the 2025 Young Researcher Award! 🏆👏 sbcf.fr/en/newslette...

Brilliant work with unprecedented level of resolution of the trypanosome axoneme!

Assistant prof / MCF post available in ProParaCyto.

March #ImageOfTheMonth goes to M.Bonhivers from MFP lab (UMR 5234). Trypanosoma brucei cell was extracted in detergent, expanded 4.2 fold using U-ExM, labelled for the microtubule cytoskeleton (magenta) and for the Hook Complex (yellow), and images usinf scanning confocal microscopy.

A massive thanks to @bladerlab.bsky.social for the opportunity to put this mSphere of influence article together! Also @dvorinlab.bsky.social, @ebertiaux.bsky.social, Brochet Lab, & @centriolelab.bsky.social for doing science that shows off why parasites are cool! journals.asm.org/doi/10.1128/...

Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin: -a new glutamylation site -far less exhaustive detyrosination Preprint here: www.biorxiv.org/content/10.1...

A video clip to see #trypanosomes in #3D #FIB-SEM Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr www.pasteur.fr/fr/journal-r...

We propose a model where the new flagellum grows in a linear manner while the old one is locked but shrinks slightly, hence requiring unlocking and compensation after cell division, leading to such multicoloured flagella following induction/deinduction/reinduction of tagged tubulin 😉

Solving 25 years of frustration, Daniel Abbühl managed to tag trypanosome tubulin and to express it in an inducible manner, hence revealing flagellum assembly! A tour-de-force! Preprint here: www.biorxiv.org/content/10.1...

Parasite of the month in Trends in Parasitology: #Trypanosoma equiperdum. By Perrine Hervé, Frédéric Bringaud and Loïc Rivière (ParaFrap Team) @cp-trendsparasitol.bsky.social

Very interesting work here: centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends rupress.org/jcb/article/...

Our paper on U-ExM for mosquito tissues (MoTissU-ExM) in BMC methods! Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social bmcmethods.biomedcentral.com/articles/10....

Bravo @pasteur.fr !!! Fier d’avoir été Pasteurien !!! (Le directeur de l’époque Christian Bréchot m’avait dit qu’on le reste toute sa vie)

Come and celebrate the exceptional career of Mark Carrington (24th March at University of Cambridge)!

Our 50th @bscb-official.bsky.social @gensocuk.bsky.social @ukcilianetwork.bsky.social e-symposium kicked off with #UExM masterclass! Mysteries & hierarchies of #tubulin #PTMs in #ConnectingCilium #Bulge #IRDs- Olivier Mercey @centriolelab.bsky.social www.embopress.org/doi/full/10.... #ukcilia2025 /1