Spoiler: lots of 😍 SPY555-FastAct live cell movies in there.

And it's not all: For an exceptional new product, an exceptional new casing! We designed new boxes that we will gradually deploy to replace our old metal boxes. 4 designs will be randomly used for each product!

Our June issue is here! rupress.org/jcb/issue/22... The cover shows a maximum intensity projection from a confocal image stack showing an angiogenic sprout emerging from an engineered microvessel. From @lakynm.bsky.social et al. of the Matthew Kutys lab: rupress.org/jcb/article/... #Cytoskeleton

Today is the Day, CenSpark650 is available! Time to get those centrioles and cilia shining under the 🔬objective! spirochrome.com/product/cens... video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650. movie credits: @cepourroy.bsky.social

😭🇫🇮🏒🇨🇭😭

📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/ #Bioimaging

Equipment check for quartz hunting. 💎 New season starts tomorrow with 8yo.

Just me again with more neurons. Happy #FluorescenceFriday. Have a great weekend.

We had such a fun ERC Synergy @erc.europa.eu kickoff workshop for our 6-year MitoContact collaborative project with our partners Kai Johnsson and Wanda Kukulski! Wonderful to share science, ideas, and play games together 💚 fantastic organization by @zimmerli.bsky.social @jclandoni.bsky.social

Excited to share our latest paper! We found that large pieces of the human genome can transfer between cells upon direct contact, endowing recipient cells with heritable phenotypic changes. @cp-cell.bsky.social (1/7) www.cell.com/cell/fulltex...

Starting materials VS products. A good yield for this afternoon!

We are very pleased to announce Prof. Paul Guichard and Dr. Virginie Hamel @centriolelab.bsky.social as joint winners of the RMS Award for Light Microscopy 🔬 👏 🏅 Huge congratulations to both! Read more about Paul and Virginie's achievements: www.rms.org.uk/resource/pro...

Dude, that's a BEE hotel, not lizard!

Casually posting this one for no particular reason! #MayTheFourth

#FluorescenceFriday material here!

Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆 The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes. evidentscientific.com/en/landing/i...

I've been fortunate to have @marcoenriquez18.bsky.social a #materialsChemist in my team. Cytoskeletal elements are often studied on their own but in reality, the #cytoskeleton is biopolymer network that is intricately intertwined. (#Award winning movie Light Microscopy Aus). @aibnatuq.bsky.social

#microtubules in orange hot, #F-actin greyscale, Fibroblast, spinning disc. @marcoenriquez18.bsky.social @aibnatuq.bsky.social #fluorescenceFriday

Localizable fluorescent metal ion indicators galore: advanced.onlinelibrary.wiley.com/doi/10.1002/...

Mesmerizing light sheet imaging of neutrophils swarming at a yeast target! The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue). This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading! www.biorxiv.org/content/10.6...

Very cool probe to label doublet MTs and therefore centrioles across different species and set ups (from live imaging, STED and expansion)!😍 www.nature.com/articles/s41...

CenSpark is out ! Congrats Gönczy team et al.

What a probe! Congratulations @cepourroy.bsky.social! We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!

CenSpark story is now published in @natchembio.nature.com. We engineered a dual-ligand fluorescent probe to selectively "light up" centrioles & cilia. Massive thank to Pierre Gönczy, @lreymond.bsky.social and @ghatzopoulos.bsky.social for the guidance. Check it out 👉: doi.org/10.1038/s415...

Organoids stained with Spirochrome probes for actin and DNA! 😍

Painted 100+ lung organoids for Easter, stained with live-cell Cell Tracker/ @spirochrome.com dyes, imaged at our @leicamicrosystems.bsky.social Viventis Deep light-sheet 🔬 at Charité (soon @Si-M - Der Simulierte Mensch) and visualized using inverted lookup tables designed by @christlet.bsky.social

Mr Blackcap (Sylvia atricapilla) singing and dancing for Mrs Blackcap just under my window! Turn the sound on, this little dude is a talented singer.

Mirror mirror on the wall who is the brightest of them all? Brightness is a key parameter for choosing fluorescent reporters in bioimaging. As tricky to assess, we present a pipeline to compare chemigenetic labels and FPs in tissue culture and #zebrafish embryos. www.biorxiv.org/content/10.6...

Beware the SPY488-FastAct rollercoaster! We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct. Find out more about SPY488-FastAct: spirochrome.com/product/spy4...

📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy! There is now a very detailed #JOVE video that explains it all step by step. Video link: dx.doi.org/10.3791/6940... Peroxi_SPY link: spirochrome.com/products/?_c... 🧪

🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.

At this high resolution, we also caught something new: Using the new #HAK-Actin #UExM probe, we saw that #Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom. ➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.

🚨New paper out today in @acs.org ACS Nano, showing how expansion microscopy can visually quantify the cell surface receptor turnover mediated by signalling & receptor organising machineries on the surfaces of endosomes, some of the smallest organelles in cells. 🧪 🔬 1/ pubs.acs.org/doi/10.1021/...

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™. These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie from preprint www.biorxiv.org/content/10.6... 🧪

5/5 The Takeaway: Flipper-TR didn't just report CMT; it identified CMT as a master switch linking physics to genetics. From development to disease, this probe proved that how "tight" a cell is matters just as much as its DNA. Read the full study: www.biorxiv.org/content/10.6...

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology

🟢FastAct™ goes green! 🟢🔬 We launch SPY488-FastAct™ a green channel live cell actin probe SPY488-FastAct allows imaging F-actin in live and fixed cells Compatible with SPY555 and SPY650 for easy 3 color imaging. spirochrome.com/product/spy4... available at our distributors in a couple of days. 🧪

Almond blossom in the garden, spring upload in full swing!

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy6... 🙏 Big thanks to @lazaresaladin.bsky.social for the images. 🧪

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list! Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript. Congratulations to the team!

Our article on how to use micropatterned presynapses-on-glass is out in its final form in the latest issue of the Journal of Neuroscience @sfnjournals.bsky.social: www.jneurosci.org/content/46/8... The cover went to the special section on social neuroscience, so here's our #notthecover attempt

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X. 🤩

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

About the "joy" of analyzing an 1H NMR spectrum of a 13C isotope labeled molecule when you didn't decouple 13C... J1, J2 and J3 coupling festival!

ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥 Thank you @j-today.bsky.social for posting this gem!

Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday

Thanks for highlighting our work on combining AI-based organelle dynamics detection in phase contrast with fluorescence! www.nature.com/ncomms/edito... @natcomms.nature.com and Nelio Rodriguez. Spearheaded by @wl-stepp.bsky.social with collaborators @jclandoni.bsky.social @maweigert.bsky.social

Powder ski day! The best of the season so far!

Smells like spring spirit!

A menagerie of COS cells for #MicroscopyMonday 🧪