Off to the lake for 7 minutes at 7°C!

A new article by @zhixingchen2.bsky.social shows that the far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM. www.nature.com/articles/s41... PKmito Deep Red is exclusively available at Spirochrome: spirochrome.com/product/pkmi...

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos. www.nature.com/articles/s42...

We’re excited to announce the opening of the Geneva Expansion Microscopy Facility (GenExM)! GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging. Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations. 👉 www.unige.ch/genexm/

Missed #FluorescenceFriday, but let's go for #GlowingSaturday! freshly out of the prep JF549 derivative for a colleague

Friday afternoon ski touring isn't a bad concept! Col de Lys from Les Paccots

Snow with lake view for the first ski tour of the year.

A new beautiful proof that Flipper-TR is an excellent reporter of membrane mechanics (and yes membrane are made of lipids ❤️‍🔥)

The unique mechanosensitive probe Flipper-TR is at the core of an amazing article by @juanmagararc.bsky.social and @rouxlab.bsky.social team of membrane tension experts. Congratulations! There is a great 🧵 explaining the key findings how and why cells build membrane tension gradients ⬇️

New paper from the @rouxlab.bsky.social on Nature Communications! We study how membrane tension is spatially organized in cells. Using the mechanosensitive probe Flipper-TR to visualize tension across the plasma membrane of adherent cells and to dissect the conditions needed for a gradient to happen

Did you know that @jcellsci.bsky.social has a new editor for neuronal cell biology? It's that guy @roylab-ucsd.bsky.social, I've heard good things about him

What's up Bluesky....here's some live-cell imaging taken in my lab

The images in the main text are also definitely worth a look!

A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct www.science.org/doi/full/10.... The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects

In our new #Preprint, we pilot a revolutionary new idea: no supplemental data (it has 21 figures, though): Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR www.biorxiv.org/content/10.1...

Got a connected bird feeder, guests quickly became regular customers! Here a couple of blue tit

With snow in the garden, that means skiing season is definitely getting closer!

Thank you @prelights.bsky.social for highlighting HAK-actin! HAK-actin is a great tool for ExM actin staining. spirochrome.com/product/hak-...

Reliable actin labelling in expansion #microscopy 🔬 Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social Read the latest #preLight from @kanishka03.bsky.social ⬇️👀 prelights.biologists.com/highlights/h...

Quite a busy day on #Bluesky ! Been a while since we have been #preLight -ed ! Discover HAK-Actin if you haven't already ;)

Cool, the HAK-actin preprint was showcased by @prelights.bsky.social A good reason to read it if you haven't yet 😜

Hike'n fondue in Valais with friends! 😋 The fall colors are splendid.

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇 www.biorxiv.org/content/10.1...

Cool paper using a new JF derivative to map synaptic proteins. And AI didn't like the generality of our general synthetic methods section (that we've used for years), so we expanded them from 2 succinct paragraphs to 1.5 gloriously unreadable pages. Better than Ambien. www.nature.com/articles/s41...

Went looking for quartz over the weekend. I was lucky to find a nice untouched pocket with well-formed crystals (after 3h of search!). The first picture shows the cluster in situ within the crack and the other one shows the same crystals as a detached piece.

Firing water rockets with the kids never gets old, especially for me! 🚀

🥝 The kiwi harvest is the last one of the year in the garden. Happily surprised by the quantity given the very moderate bloom in spring.

🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩‍🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️ D-CHAB @ethz.ch www.nature.com/articles/s41...

More information about Flipper-TR on our website spirochrome.com/product/flip...

👉🦠Interested in bacterial membrane tension? Our membrane tension probe Flipper-TR is a top tool for the job! A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension. 🔗 doi.org/10.1073/pnas... 🧪

👻 Spooky cookies 🎃

Find out how SPY650-FastAct_X can help your research here: spirochrome.com/product/spy6...

Live cell ACTIN imaging has never been easier with SPY650-FastAct_X! 1. Add the probe to the cells 2. Image 1h later SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell. image credits: @stephanlab.bsky.social

I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)

There are only a few things in life cooler than crawling inside a lava tunnel. Incredible geological formations, narrow sections followed by rooms with over 3m high ceilings and pilars.

It was a tremendous pleasure to have @zhixingchen2.bsky.social visiting us today in Frankfurt and listen to his great science on developing photostable and gentle fluorophores for microscopy. Thank you, Zhixing, for this inspiring seminar! 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social

An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable. Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!

🚨 New preprint out! “Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies. www.biorxiv.org/content/10.1...

Cold blurry colors for a cold morning hike at dawn. We hoped to hear the deer rut calls (Brame du cerf in French) but the mountain remained silent today.

Quince/apple gelly workshop. Currently extracting the polymers responsible for the gelation before filtration, sugar addition, evaporation of 40% the solvent and aliquoting. Kitchen chemistry!

Sichuan pepper from the garden, that's the 2nd year I get some berries. Smells incredibly good.

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯 doi.org/10.1101/2025... #imaging

Got myself a new pair of mountain buddies after the passing of my 10+ years old pals.

🤯 Amazing findings in this manuscript. And look at the live imaging of embryos for over 24h 😍

Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...

My initial assessment of this paper ⬇️ was brief, so let’s have a better look at it: 🧵

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF