Hike'n fondue in Valais with friends! 😋 The fall colors are splendid.

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇 www.biorxiv.org/content/10.1...

Cool paper using a new JF derivative to map synaptic proteins. And AI didn't like the generality of our general synthetic methods section (that we've used for years), so we expanded them from 2 succinct paragraphs to 1.5 gloriously unreadable pages. Better than Ambien. www.nature.com/articles/s41...

Went looking for quartz over the weekend. I was lucky to find a nice untouched pocket with well-formed crystals (after 3h of search!). The first picture shows the cluster in situ within the crack and the other one shows the same crystals as a detached piece.

Firing water rockets with the kids never gets old, especially for me! 🚀

🥝 The kiwi harvest is the last one of the year in the garden. Happily surprised by the quantity given the very moderate bloom in spring.

🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩‍🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️ D-CHAB @ethz.ch www.nature.com/articles/s41...

More information about Flipper-TR on our website spirochrome.com/product/flip...

👉🦠Interested in bacterial membrane tension? Our membrane tension probe Flipper-TR is a top tool for the job! A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension. 🔗 doi.org/10.1073/pnas... 🧪

👻 Spooky cookies 🎃

Find out how SPY650-FastAct_X can help your research here: spirochrome.com/product/spy6...

Live cell ACTIN imaging has never been easier with SPY650-FastAct_X! 1. Add the probe to the cells 2. Image 1h later SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell. image credits: @stephanlab.bsky.social

I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)

There are only a few things in life cooler than crawling inside a lava tunnel. Incredible geological formations, narrow sections followed by rooms with over 3m high ceilings and pilars.

It was a tremendous pleasure to have @zhixingchen2.bsky.social visiting us today in Frankfurt and listen to his great science on developing photostable and gentle fluorophores for microscopy. Thank you, Zhixing, for this inspiring seminar! 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social

An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable. Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!

🚨 New preprint out! “Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies. www.biorxiv.org/content/10.1...

Cold blurry colors for a cold morning hike at dawn. We hoped to hear the deer rut calls (Brame du cerf in French) but the mountain remained silent today.

Quince/apple gelly workshop. Currently extracting the polymers responsible for the gelation before filtration, sugar addition, evaporation of 40% the solvent and aliquoting. Kitchen chemistry!

Sichuan pepper from the garden, that's the 2nd year I get some berries. Smells incredibly good.

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯 doi.org/10.1101/2025... #imaging

Got myself a new pair of mountain buddies after the passing of my 10+ years old pals.

🤯 Amazing findings in this manuscript. And look at the live imaging of embryos for over 24h 😍

Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...

My initial assessment of this paper ⬇️ was brief, so let’s have a better look at it: 🧵

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF

Bringing some more ExM to #FluorescenceFriday with what appears to be a frustrated cell division in fibroblasts. More details in Alt-text.

Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition! His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin. www.nikonsmallworld.com/galleries/20... #FluorescenceFriday

Congrats to our PhD student Louis Romette, among the winners in this year's @healthcare.nikon.com Nikon Small World competition! His hard work optimizing long-term live-cell imaging of developing neurons makes for mesmerizing movies (and lots of insights) www.nikonsmallworld.com/galleries/20...

Logic-gating the HaloTag system with Conditional-Halo-ligator 'CHalo' reagents by Oliver Thorn-Seshold and team: www.biorxiv.org/content/10.1...

I love harvesting mushrooms! Those will be pickled for later, when Raclette temperatures arrive!

Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation - nice work showing that an F-actin cage-like structure surrounding oocytes polar MTOCs relies on MYO10 and MYO7B. www.nature.com/articles/s41...

Amazing achievement! Congratulations Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍

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SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...

The final version of our multispectral microscopy manuscript is now online at Nature Photonics: www.nature.com/articles/s41... The main additions are some further comparisons to yet more unmixing algorithms, in which we find that our algorithm is still the most accurate.

We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:

Its raining #Ichthyos ... Hallelujah! #Lattice #LightSheet #Ichthyosporeans #Creolimax #Cellularization #ProtistsOnSky

Full UV-VIS spectrum apero incoming. Choose your wavelength!

📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure). 🔗 View the preprint article: bit.ly/47xC8NJ #Microscope #Imaging #Fluorescence

HAK-actin™ was developed in the labs of @centriolelab.bsky.social and @dudinlab.bsky.social. Find out more about HAK-actin™ in the BioRxiv manuscript: doi.org/10.1101/2025... We are grateful to @dudinlab.bsky.social for providing the creolimax frangantissima data used in the movie

🧬 New tool for U-ExM! HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy. Check it out at Spirochrome! #UExM #Actin #Cytoskeleton #SuperResolution

For all of you #Actin folks ... It's out ! Happy to help at anypoint

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy • Stronger signal via post-expansion labeling • Broad cell lines & species compatibility • Endogenous actin labeling • Can be multiplexed with other targets spirochrome.com/product/hak-...

So honored to be awarded the ERC Starting Grant! I am very grateful for this support and looking forward to more exciting #lipidtime 🥳 Also, we will have positions available next year. So stay tuned!

Nice cell division observed on Zeiss LLS7 yesterday! Sample: @romainguiet.bsky.social

Same cell division as posted below, rendered with bene51.github.io/3Dscript/

A stunning application of SPY650-FastAct_X (actin)/SPY555-DNA (nuclei) staining iPSC cells and imaged by light sheet microscopy at UCSF. Thank you ‪@microtubule.bsky.social‬ for sharing it!