Kai Johnsson
@kjohnsson.bsky.social
๐ค 571
๐ฅ 117
๐ 11
Chemical biologist @ Max Planck Institut for Medical Research in Heidelberg
Localizable fluorescent metal ion indicators galore:
advanced.onlinelibrary.wiley.com/doi/10.1002/...
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Localizable Fluorescent Metal Ion Indicators With Tunable Colors
A modular platform enables the generation of fluorescent metal ion indicators with tunable emission colors and precise subcellular localization. Conjugation to self-labeling proteins yields bright, c...
https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.75402
25 days ago
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A high-affinity split-HaloTag for live-cell protein labeling. And much more.
www.nature.com/articles/s41...
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A high-affinity split-HaloTag for live-cell protein labeling
Nature Communications - Lin and colleagues present high-affinity split-HaloTag pairs for protein tagging and multiplexed labelling. This versatile system allows protein visualisation with diverse...
https://www.nature.com/articles/s41467-026-71032-8.epdf?sharing_token=SwhuERz3x4x2PtMpZqm37NRgN0jAjWel9jnR3ZoTv0NXCWvaKYk-Xuv2MiEQauHVVSmwrm7JkH9-xVP0jtLIGprbOnnbhq4EY96uHN8z7403Rcd4G5Nl05giDgwf3hq5Fjl0jf7e8f_4EjinQx-8kLaQuydUy4n0PtwFaPbr_PQ%3D
about 2 months ago
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A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling.
www.biorxiv.org/content/10.6...
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https://www.biorxiv.org/content/10.64898/2026.03.05.709795v1
2 months ago
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A good end to 2025: rhodamine-binders for bioimaging in JACS:
pubs.acs.org/doi/10.1021/...
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Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins
Rhodamine dyes conjugated to targeting ligands can yield exceptionally bright fluorescent probes for live-cell imaging. However, the limited permeability of such rhodamine derivatives restricts their ...
https://pubs.acs.org/doi/10.1021/jacs.5c18083
5 months ago
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Molecular recording of cellular protein kinase activity with chemical labeling:
www.nature.com/articles/s41...
Congratulations to De-en Sun and the entire team.
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Molecular recording of cellular protein kinase activity with chemical labeling - Nature Chemical Biology
Molecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous cell populations and in vivo.
https://www.nature.com/articles/s41589-025-01949-6
10 months ago
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Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:
www.biorxiv.org/content/10.1...
Congratulations to Julian Kompa and the entire team.
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Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging
We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...
https://www.biorxiv.org/content/10.1101/2025.07.06.663254v1
10 months ago
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Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
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SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kรผhn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
https://www.nature.com/articles/s41589-025-01942-z
11 months ago
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Please check out our new high-affinity split-HaloTag for live-cell protein labeling
www.biorxiv.org/content/10.1...
Congratulations to Yin-Hsi Lin!
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https://www.biorxiv.org/content/10.1101/2025.06.15.659771v1
11 months ago
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Improving split-HaloTag through computational protein engineering:
onlinelibrary.wiley.com/doi/10.1002/...
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Improving splitโHaloTag through computational protein engineering
Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual ....
https://onlinelibrary.wiley.com/doi/10.1002/pro.70123
about 1 year ago
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Check out our new fluorescent probe for imaging f-actin dynamics: ๐ฆ๐ถ๐ฅ-๐ซ๐๐ฐ๐๐ถ๐ป: ๐ ๐ณ๐น๐๐ผ๐ฟ๐ฒ๐๐ฐ๐ฒ๐ป๐ ๐ฝ๐ฟ๐ผ๐ฏ๐ฒ ๐ณ๐ผ๐ฟ ๐ถ๐บ๐ฎ๐ด๐ถ๐ป๐ด ๐ฎ๐ฐ๐๐ถ๐ป ๐ฑ๐๐ป๐ฎ๐บ๐ถ๐ฐ๐ ๐ถ๐ป ๐น๐ถ๐๐ฒ ๐ฐ๐ฒ๐น๐น๐
www.biorxiv.org/content/10.1...
Thank you
@veselin-nasufovic.bsky.social
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SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells
Imaging actin-dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural-product based fluorescent probes for actin filaments affec...
https://www.biorxiv.org/content/10.1101/2025.02.04.636537v1
over 1 year ago
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