Here is a small pre-print from Arndt Group @FSUJena about SAR which led to the development of SiR-XActin. Thanks to everyone involved! chemrxiv.org/engage/chemr...

Happy to see what XActin probes can do (Spy555-FastAct_X) <3

📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳 www.nature.com/articles/s41...

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging: www.biorxiv.org/content/10.1... Congratulations to Julian Kompa and the entire team.

Check out our new fluorescent probe for imaging f-actin dynamics: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 www.biorxiv.org/content/10.1... Thank you @veselin-nasufovic.bsky.social

I was impressed with SiR-XActin: similar labeling of F-actin as Lifeact, didn't detectably alter actin dynamics, and because it can remain in solution while imaging, photobleaching is not a major concern (Below, LA required correction, SiR-Xactin did not). Happy to have played a small part in this!

Can you spot the difference? 🔬 SiR-actin labeling can be cytotoxic and affects actin dynamics - while the new XActin probes do not! Developed by @veselin-nasufovic.bsky.social and perfect for time-resolved and STED imaging without the need for genetic manipulation. www.biorxiv.org/content/10.1...

New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social: www.biorxiv.org/content/10.1...

1/5 🚀 Excited to share our latest preprint from: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 on #bioRxiv! www.biorxiv.org/content/10.1... #JohnssonLab and #ArndtLab with valued collaborators

Thrilled to share with you our work on covalent probes! By introducing a biocompatible sulfonium-based cleavable linker, we developed a series of fluorescent probes that can label covalently endogenous tubulin in various cell lines. www.biorxiv.org/content/10.1... #Chembio #FluorescenceFriday

I would like to draw your attention to this job: Scientist Proteomics/Chemical Biology talent.bayer.com/careers/job/... Chemical Biology, Imaging and Omics group is really great please to work. We will be very happy to get new motivated colleague.

Cool paper from Cravatt lab: Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes: www.cell.com/cell-chemica...

The "Fluorescent Probes, Sensors and Fluorophores" starter pack got substantial additions worth a new look. Please raise you hand 👋 in the replies if you want to be added too. go.bsky.app/MtgJE4w