👉🦠Interested in bacterial membrane tension? Our membrane tension probe Flipper-TR is a top tool for the job! A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension. 🔗 doi.org/10.1073/pnas... 🧪

Find out how SPY650-FastAct_X can help your research here: spirochrome.com/product/spy6...

Live cell ACTIN imaging has never been easier with SPY650-FastAct_X! 1. Add the probe to the cells 2. Image 1h later SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell. image credits: @stephanlab.bsky.social

Live cell ACTIN imaging has never been easier with SPY650-FastAct_X! 1. Add the probe to the cells 2. Image 1h later SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell. image credits: @stephanlab.bsky.social

An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable. Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯 doi.org/10.1101/2025... #imaging

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯 doi.org/10.1101/2025... #imaging

SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition! His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin. www.nikonsmallworld.com/galleries/20... #FluorescenceFriday

HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol. Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-... Image credits: Olivier Mercey, UNIGE

HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol. Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-... Image credits: Olivier Mercey, UNIGE

Amazing achievement! Congratulations Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍

🚨Publication alert🚨 My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...

📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure). 🔗 View the preprint article: bit.ly/47xC8NJ #Microscope #Imaging #Fluorescence

For all of you #Actin folks ... It's out ! Happy to help at anypoint

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy • Stronger signal via post-expansion labeling • Broad cell lines & species compatibility • Endogenous actin labeling • Can be multiplexed with other targets spirochrome.com/product/hak-...

🧬 New tool for U-ExM! HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy. Check it out at Spirochrome! #UExM #Actin #Cytoskeleton #SuperResolution

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy • Stronger signal via post-expansion labeling • Broad cell lines & species compatibility • Endogenous actin labeling • Can be multiplexed with other targets spirochrome.com/product/hak-...

A stunning application of SPY650-FastAct_X (actin)/SPY555-DNA (nuclei) staining iPSC cells and imaged by light sheet microscopy at UCSF. Thank you ‪@microtubule.bsky.social‬ for sharing it!

A new #UExM probe for #Actin! Fun times thinking/developing/testing with @centriolelab.bsky.social & @lreymond.bsky.social ! Congrats to all ! If interested in #UExM, or #HAK-Actin, feel free to reach out ! Soon available at @spirochrome.com #Expansion #Microscopy #ProtistsOnSky

Getting a bright & reliable actin staining in expansion microscopy was a tough nut until today! The team gathered around @centriolelab.bsky.social found a hak: HAK-actin, a new probe for U-ExM based protocols revealed in a new BioRxiv manuscript. HAK-actin will soon be available at Spirochrome!

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1... Soon at @spirochrome.com Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

SPY650-FastAct_X (red) & SPY555-tubulin (blue) teaming up in this astrocyte to highlight mitochondria (white) and cytoskeleton interactions. Thank you @aaandmoore.bsky.social‬ for sharing this amazing movie.

Very happy to see our preprint on how the mechanosensitive ion channel Piezo1 controls intercellular junction maturation by balancing cortex and membrane tension now published in @jcellsci.bsky.social Congratulations to Ahsan & team! journals.biologists.com/jcs/article/...

Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.

Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.

Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday

Happy to see what XActin probes can do (Spy555-FastAct_X) <3

🤩 SPY555-FastAct_X in action in a Moth developing wing, inside the chrysalis & live! 🤯 Just wow!

If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go! Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy. #snouty

🌑Goodbye bleaching, hello brightness! ☀️ We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes! ✅ Highly photoresistant, no photoblueing ✅ Outstanding brightness ✅ Cell permeable ✅ Outperform best in class fluorophores

🌑Goodbye bleaching, hello brightness! ☀️ We're thrilled to introduce two new Halotag ligands featuring @zhixingchen2.bsky.social fantastic photoresistant BD-dyes! ✅ Highly photoresistant, no photoblueing ✅ Outstanding brightness ✅ Cell permeable ✅ Outperform best in class fluorophores

PKmem probes for gentle membrane imaging are now published in PNAS! Congratulations for these exceptional probes. Available at Spirochrome!

The first UK Peroxisome Network Meeting in Exeter was a great success! We're grateful to the organizers for giving us the opportunity to support this great event. Triana Amen presented her amazing work on the development of Peroxi_SPY probes for peroxisome imaging. Rendez-vous at the next edition!

Out in Nat Chem Bio today: "Molecular recording of cellular protein kinase activity with chemical labeling" from @kjohnsson.bsky.social lab. doi.org/10.1038/s415... CPY-CA Halotag ligand used to label Kinprola throughout the study is available at Spirochrome: spirochrome.com/product/cpy-...

I love the shine of Silicon Rhodamine in the morning sun ✨

Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.

Our mechanosensitive Flipper-TR™ probes at work in a stunning article by Z. Chen Et al. from the University of western Australia. The study unveils a PM-ER mechanotransduction mechanism in response to external strain that dose-dependently regulates cellular metabolism. doi.org/10.1126/scia...

Our mechanosensitive Flipper-TR™ probes at work in a stunning article by Z. Chen Et al. from the University of western Australia. The study unveils a PM-ER mechanotransduction mechanism in response to external strain that dose-dependently regulates cellular metabolism. doi.org/10.1126/scia...

Out in print! Using ancestral sequence reconstruction 🦖, in vivo characterization, and 🔬 analysis, we identified key residues in the mitochondrial protein #Mic60 that affect #respiration in #yeast. Since the preprint, we added some cool findings. 🧵⬇️ onlinelibrary.wiley.com/doi/10.1002/...

Dear Yeast friends: Did you know PKmito ORANGE labels nicely yeast cells mitochondria! The image is from in a recent article by @fmcbenning.bsky.social : doi.org/10.1002/pro.... PKmito ORANGE is a gentle probe for mitochondria imaging with reduced phototoxicity spirochrome.com/product/pkmi...

Let's start the week with this movie kindly shared by @loxstoplox.bsky.social The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.

Let's start the week with this movie kindly shared by @loxstoplox.bsky.social The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.

New preprint by @kjohnsson.bsky.social lab! A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing. The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.

Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1... Congratulations to Yin-Hsi Lin!

We are thrilled to continue our great collaboration with @zhixingchen2.bsky.social and Genvivo Tech. New products will be launched soon!

🚨 Last week to submit abstracts for talks & posters! Show your research, win prizes & enjoy surprises 🎉 Register here: ‘’ shorturl.at/eOmJt ‘’

Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right). Stain: @spirochrome.com SiR-Tubulin Happy #FlourescenceFriday!

Thank you @axonsalex.bsky.social, this movie is mind blowing! We are immensely proud that, thanks to you, our SPY555-FastAct actin probe is represented at the Nikon Small World in Motion competition! We'll keep our fingers crossed for the competition 🤞