Home microscope project - high NA Phase contrast with the fun Lowa objective Lomo inverted with the Lomo 1.25 NA water inversion phase contrast Need to add light source and camera and phase annulus

📢 We're recruiting AI Scientists at HHMI Janelia. I'm biased, but these are exceptional opportunities. You'd join as an independent scientist with access to excellent computational infrastructure, some of the best biological data in the world, and a vibrant collaborative environment.

Expansion microscopy rocks

Ultrastructure of dopaminergic varicosities revealed by cryo-correlative light and electron microscopy. New study from Matthew Domenic Lycas (@lycasworks.bsky.social), Simon Erlendsson et al. @ucph.bsky.social: rupress.org/jcb/article/... #Neuroscience #Organelles #StructuralBiology #cryoCLEM

Got my pictures back

This mesmerizing timelapse captures the nonstop motion inside an animal cell. Red/orange streaks are the growing ends of microtubules, tiny “highways” that can rapidly assemble and disassemble to move proteins & other molecules within the cell. 📸: Andy Moore, HHMI’s Janelia Research Campus

My first corresponding author 🔬🎸🦾 rupress.org/jcb/article/... Ever wonder what the ultrastructure of dopaminergic neuron presynaptic sites looks like? Using cryo-CLEM/ET we observed the wild changes that happen at these sites when the neurons fire or are more quiet. In the latest @jcb.org

In @jcb.org, @lycasworks.bsky.social, Erlendsson et al. @ucph.bsky.social develop a #cryoCLEM workflow to characterize the ultrastructure of dopaminergic varicosities. rupress.org/jcb/article/... #Neuroscience #Organelles #StructuralBiology #Microscopy

@lycasworks.bsky.social, Erlendsson et al. @ucph.bsky.social develop a #cryoCLEM workflow to characterize the ultrastructure of dopaminergic varicosities. rupress.org/jcb/article/... #Neuroscience #Organelles #StructuralBiology #Microscopy

Our preprint on Antarctic fish cell cultures is out now! Loads of pretty nice super-resolution images. Read it here: www.biorxiv.org/content/10.6...

www.protocols.io/view/cryo-ex... New protocol alert!! Cryo-expansion microscopy of C. elegans and Tardigrades using the high pressure freezer!

We're hiring! 🔬🏔️ Looking for curious, open-minded, and multidisciplinary #PhD candidates to research at the intersection of #mitochondria, #metabolism, #imaging & #physiology, with the #Alps as a backdrop. Sounds like you or someone you know? Link below! @fbm-unil.bsky.social @unil.bsky.social

Quantitative Imaging: From Acquisition to Analysis (QICM), April 6 - April 21, 2026 Thank you to all of the participants and vendors that supported the course! #cshlcourses #cshl Photo Credit: Max Brambach, Eva de la Serna, and Federico Gasparoli (2026 Teaching Assistants) @qiatcshl.bsky.social

So thrilled to be part of Quantitative Imaging @qiatcshl.bsky.social Such an amazing course and now we got a whole new generation of expansion microscopy enthusiasts!

@lycasworks.bsky.social showing expansion microscopy followed by some hands on expansion exercises.

The Landoni Lab opens this summer at UNIL! @fbm-unil.bsky.social 🔬 Quantitative #imaging + systems biology to decipher how #metabolic architecture & #mtDNA dynamically shape tissue development, #aging and #mitodisease. Beyond excited to fire up the lasers, open positions soon! 🧪 #mitochondria #newPI

Super Res Sunday! And we are doing all the techniques:)

Hari Shroff giving the QI keynote on structural illumination microscopy, adaptive optics and new biosensors. Wow!

Light sheet lab. We image anything from cells to plants to shrimp. @fedegasparoli.bsky.social @excitedemissions.bsky.social are running the machines!

Light Sheet Saturday is here. @jencwaters.bsky.social and @talley.codes lecture on how to improve your photon budget by illuminating from the side.

AI for image analysis. @florianjug.bsky.social @bethcimini.bsky.social @damiandn.bsky.social are showing off the power of the machines:)

Pizza and image analysis. Because there’s no hackin‘ without snackin‘. @damiandn.bsky.social @bethcimini.bsky.social

Cake and image analysis. True QI bliss:) @damiandn.bsky.social @bethcimini.bsky.social @emiglietta.bsky.social teaching 3D segmentation.

Looking for samples for our lightsheet microscope and expansion microscopy lab, @fedegasparoli.bsky.social and @lycasworks.bsky.social go on an expedition during low tide.

Hands on TIRF microscopy and machine learning segmentation has students up all night.

Hard to decide what’s better the beautiful weather or the lectures by @talley.codes and @jencwaters.bsky.social on confocal microscopy. (it’s obviously the lectures)

We are getting serious about analysis. Today Hunter Elliott and @florianjug.bsky.social introduce machine learning and AI. It’s not magic, it’s computers.

Today’s lectures on live cell imaging and fluorescent proteins by @jencwaters.bsky.social and @talley.codes Every photon counts!

We tried other brands but nothing works quite like a Guinness for our light sheet microscope. Future collab?

Today Hunter Elliott is lecturing on image processing. Key take-home before lunch: There is no free lunch:)

This morning‘s lecture is about Koehler illumination. Pop quiz: What happens when you close the condenser aperture?

Check out the fantastic perspective by @jellevda.bsky.social & @prudentlab.bsky.social on our #pearling work, out in the same @science.org issue! www.science.org/doi/10.1126/...

The longstanding mystery for how mitochondria segregate and position their DNA has been solved! Read all about it: www.science.org/doi/10.1126/... @jclandoni.bsky.social Here is Juan and I at the 4th of July parade at MBL physiology

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort. www.biorxiv.org/cgi/content/...

All set for some expanding

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

Found the unbroken link: www.biorxiv.org/content/10.6...

@lycasworks.bsky.social developed quantitative expansion (qExM) to estimate endogenous protein quantities in situ. His ExM protocols offer epitope preservation and accessibility so that standard Western Blot antibodies can be used. Also, check out the cristae, supercomplexes, specialized mitos 🤩

🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem. leb-epfl.github.io/basic_traini...

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026

Seal taking a nap off the coast of Cape Town

A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social. Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Thrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩. www.nature.com/articles/s41... @msarscentre.bsky.social 🧠✨🧬🌊🪼🧽

New preprint showing how the membrane-associated periodic skeleton (MPS) restricts endocytosis in neurons: www.biorxiv.org/content/10.6... It nicely confirms our work from last year (science.org/doi/10.1126/science.ado2032) and extends it to other compartments in more mature neurons

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

FLIM at the Biozentrum 🔬