PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem. leb-epfl.github.io/basic_traini...

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026

Seal taking a nap off the coast of Cape Town

A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social. Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Thrilled to see our review article "The evolutionary origins of synaptic proteins" highlighted on the cover of Nature Reviews Neuroscience 🤩. www.nature.com/articles/s41... @msarscentre.bsky.social 🧠✨🧬🌊🪼🧽

New preprint showing how the membrane-associated periodic skeleton (MPS) restricts endocytosis in neurons: www.biorxiv.org/content/10.6... It nicely confirms our work from last year (science.org/doi/10.1126/science.ado2032) and extends it to other compartments in more mature neurons

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

FLIM at the Biozentrum 🔬

Tardigrades looking BIG

We are highlighting Jennifer Waters, a @focalplane.bsky.social Scientific Advisory Board member, Director of CITE, Harvard Medical School, and creator of MicroList (now featured in FocalPlane) and Microtutor, as an extraordinary biologist. #100biologists @jencwaters.bsky.social

Expansion microscopy of a tardigrade I caught outside with the objective I got on eBay

Bought more objectives on eBay

Putting the eBay objective to good use

Super resolution is super T cell Mitochondria labelled for MT-CO1

Shout out eBay, part 1 of the home microscope

Tardigrades in the mail

Silver metal at Swiss BJJ Championship

So happy to be at #GEF25 thank you to the organisers and I can’t wait for all our future developments in expansion microscopy

#GEF2025 Such a fantastic conference 🥳🥳🥳

More Iterative expansion for a specific inner mitochondrial membrane protein

Iterative expansion microscopy for protein on the mitochondria inner membrane

Great Cormorant, Preverenges Switzerland. Go birds

Happy Physio!

Go Physio!!!

Happy Physio!

New tattoo: Cajal‘s microscope. His illustrations of cortical neurons, hippocampal neurons, synapses, and the brain. Shout out to Phil from the tattoo Lab for his brilliant work

Cristae

Mitochondria!

Phase! Contrast!

Just testing out DIC on my cheek cells for a writing break

Happy Expansion Microscopy Day at Quantitative Imaging @qiatcshl.bsky.social

Our latest preprint www.biorxiv.org/content/10.1..., describes Willi Stepp’s project to make smart microscopy even gentler by doing event detection in phase contrast. We developed neural networks to detect mito-LD and mito-lysosome contacts, as well as mitochondrial pre-fission constrictions.

@fedegasparoli.bsky.social science! @qiatcshl.bsky.social

Great imaging today at Quantitative Imaging at CSHL @qiatcshl.bsky.social #expansion

Expansion microscopy of a tardigrade

Demonstrating how local environment impacts fluorescence 🔬🧪🥼@qiatcshl.bsky.social

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨

If you are looking to do GPU deconvolution in Fiji checkout the new updates to CLIJ (OpenCL for ImageJ) deconvolution. @nkiaru.bsky.social has done updates to the tiled deconvolution and integrated with BigDataViewer, and I've added a new script for batch processing. forum.image.sc/t/clij-openc...

@science.org A subcellular map of translational machinery composition and regulation at the single-molecule level | Science www.science.org/doi/10.1126/...

Fluorescence Freitag!

Tardigrade

Tardigrades and Rotifers

Great place for microscopy

I'm so happy to share our work discussing nucleoid trafficking inside the mitochondrion and mitochondrial network, led by the amazing @jmacuada.bsky.social Available in @cp-trendscellbio.bsky.social www.cell.com/trends/cell-... #mito #mitochondria

The magic of the live #mito straight from the microscope 🔬 for #FluorescenceFriday - brought to you by #SYBRgold 🟢 and #PKMO 🟠 @spirochrome.com @carolinklose.bsky.social @lycasworks.bsky.social

Awesome modelling by @askeejdrup.bsky.social !! Great Investigation to links between dopamine dynamics and DAT nanodomains!!! In @elife.bsky.social: Computational modelling identifies key determinants of subregion-specific dopamine dynamics in the striatum doi.org/10.7554/eLif...