FINALLY a histogram in napari! ♥️

Our kind of fireworks: fruit fly neurons stacked in a rapid succession of rainbow colors. This video shows a small sampling of the more than 135,000 neurons in a typical fruit fly's brain, imaged by researchers at our Janelia Research Campus using multicolor flipout technique 🎆

These stunning single-celled organisms can shape-shift & regenerate. Their trumpet-like shape inspired their name: stentor, after the herald in Greek mythology known for his booming voice. 📸: Confocal microscopy by Igor Siwanowicz, HHMI’s Janelia Research Campus

The once-obscure Danionella fish takes center stage as a model research organism in a major new project funded by the Howard Hughes Medical Institute. Learn more: https://scim.ag/3QFj4XM

One of the world's leading brain research centers is shifting away from fruit flies and toward a tiny, transparent fish. The goal: to understand how brains control the behavior of an animal or human. n.pr/4vV8dYM

Janelia is built for this moment. Starting today, we're taking on 2 big bets: cracking how a vertebrate brain generates behavior, & building AI-in-the-Loop Discovery — a new way of doing biology. Tiny transparent fish + one of neuroscience’s biggest questions? Let's go: hhmi.news/2BigBets

And so it begins! Day 1, Lecture 0 of the first DL for Microscopy Image Analysis course at @hhmijanelia.bsky.social. Slides from @florianjug.bsky.social, presented by our TA Federico Carrara. #dljanelia

Home microscope project - high NA Phase contrast with the fun Lowa objective Lomo inverted with the Lomo 1.25 NA water inversion phase contrast Need to add light source and camera and phase annulus

📢 We're recruiting AI Scientists at HHMI Janelia. I'm biased, but these are exceptional opportunities. You'd join as an independent scientist with access to excellent computational infrastructure, some of the best biological data in the world, and a vibrant collaborative environment.

Expansion microscopy rocks

Ultrastructure of dopaminergic varicosities revealed by cryo-correlative light and electron microscopy. New study from Matthew Domenic Lycas (@lycasworks.bsky.social), Simon Erlendsson et al. @ucph.bsky.social: rupress.org/jcb/article/... #Neuroscience #Organelles #StructuralBiology #cryoCLEM

Got my pictures back

This mesmerizing timelapse captures the nonstop motion inside an animal cell. Red/orange streaks are the growing ends of microtubules, tiny “highways” that can rapidly assemble and disassemble to move proteins & other molecules within the cell. 📸: Andy Moore, HHMI’s Janelia Research Campus

My first corresponding author 🔬🎸🦾 rupress.org/jcb/article/... Ever wonder what the ultrastructure of dopaminergic neuron presynaptic sites looks like? Using cryo-CLEM/ET we observed the wild changes that happen at these sites when the neurons fire or are more quiet. In the latest @jcb.org

In @jcb.org, @lycasworks.bsky.social, Erlendsson et al. @ucph.bsky.social develop a #cryoCLEM workflow to characterize the ultrastructure of dopaminergic varicosities. rupress.org/jcb/article/... #Neuroscience #Organelles #StructuralBiology #Microscopy

@lycasworks.bsky.social, Erlendsson et al. @ucph.bsky.social develop a #cryoCLEM workflow to characterize the ultrastructure of dopaminergic varicosities. rupress.org/jcb/article/... #Neuroscience #Organelles #StructuralBiology #Microscopy

www.protocols.io/view/cryo-ex... New protocol alert!! Cryo-expansion microscopy of C. elegans and Tardigrades using the high pressure freezer!

We're hiring! 🔬🏔️ Looking for curious, open-minded, and multidisciplinary #PhD candidates to research at the intersection of #mitochondria, #metabolism, #imaging & #physiology, with the #Alps as a backdrop. Sounds like you or someone you know? Link below! @fbm-unil.bsky.social @unil.bsky.social

Quantitative Imaging: From Acquisition to Analysis (QICM), April 6 - April 21, 2026 Thank you to all of the participants and vendors that supported the course! #cshlcourses #cshl Photo Credit: Max Brambach, Eva de la Serna, and Federico Gasparoli (2026 Teaching Assistants) @qiatcshl.bsky.social

So thrilled to be part of Quantitative Imaging @qiatcshl.bsky.social Such an amazing course and now we got a whole new generation of expansion microscopy enthusiasts!

@lycasworks.bsky.social showing expansion microscopy followed by some hands on expansion exercises.

The Landoni Lab opens this summer at UNIL! @fbm-unil.bsky.social 🔬 Quantitative #imaging + systems biology to decipher how #metabolic architecture & #mtDNA dynamically shape tissue development, #aging and #mitodisease. Beyond excited to fire up the lasers, open positions soon! 🧪 #mitochondria #newPI

Super Res Sunday! And we are doing all the techniques:)

Hari Shroff giving the QI keynote on structural illumination microscopy, adaptive optics and new biosensors. Wow!

Light sheet lab. We image anything from cells to plants to shrimp. @fedegasparoli.bsky.social @excitedemissions.bsky.social are running the machines!

Light Sheet Saturday is here. @jencwaters.bsky.social and @talley.codes lecture on how to improve your photon budget by illuminating from the side.

AI for image analysis. @florianjug.bsky.social @bethcimini.bsky.social @damiandn.bsky.social are showing off the power of the machines:)

Pizza and image analysis. Because there’s no hackin‘ without snackin‘. @damiandn.bsky.social @bethcimini.bsky.social

Cake and image analysis. True QI bliss:) @damiandn.bsky.social @bethcimini.bsky.social @emiglietta.bsky.social teaching 3D segmentation.

Looking for samples for our lightsheet microscope and expansion microscopy lab, @fedegasparoli.bsky.social and @lycasworks.bsky.social go on an expedition during low tide.

Hands on TIRF microscopy and machine learning segmentation has students up all night.

Hard to decide what’s better the beautiful weather or the lectures by @talley.codes and @jencwaters.bsky.social on confocal microscopy. (it’s obviously the lectures)

We are getting serious about analysis. Today Hunter Elliott and @florianjug.bsky.social introduce machine learning and AI. It’s not magic, it’s computers.

Today’s lectures on live cell imaging and fluorescent proteins by @jencwaters.bsky.social and @talley.codes Every photon counts!

We tried other brands but nothing works quite like a Guinness for our light sheet microscope. Future collab?

Today Hunter Elliott is lecturing on image processing. Key take-home before lunch: There is no free lunch:)

This morning‘s lecture is about Koehler illumination. Pop quiz: What happens when you close the condenser aperture?

Check out the fantastic perspective by @jellevda.bsky.social & @prudentlab.bsky.social on our #pearling work, out in the same @science.org issue! www.science.org/doi/10.1126/...

The longstanding mystery for how mitochondria segregate and position their DNA has been solved! Read all about it: www.science.org/doi/10.1126/... @jclandoni.bsky.social Here is Juan and I at the 4th of July parade at MBL physiology

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort. www.biorxiv.org/cgi/content/...

All set for some expanding

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

Found the unbroken link: www.biorxiv.org/content/10.6...

@lycasworks.bsky.social developed quantitative expansion (qExM) to estimate endogenous protein quantities in situ. His ExM protocols offer epitope preservation and accessibility so that standard Western Blot antibodies can be used. Also, check out the cristae, supercomplexes, specialized mitos 🤩

🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

I finally got around to writing the discussion section of our lab's basic training course on live cell #microscopy. I explain the tradeoffs involved in designing an imaging experiment and the art of thinking of the experiment as an optimization problem. leb-epfl.github.io/basic_traini...

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026