GORGEOUS data from Laura Smithson for #MicroscopyMonday! 🏆 Our #NESM Fall Symposium Poster Winner (Brown Univ., de Graffenried Lab) used customized #FIB-SEM to reconstruct the T. brucei cytoskeleton at 4 nm res — 9,000+ images & 660 GB. @brown.edu @aicjanelia.bsky.social #Microscopy #VolumeEM #EM

It’s great to see this document supporting colocalization via Phasor analysis of HSI data, now available on confocal microscopes. Thanks to @scopeshifu.bsky.social and @aicjanelia.bsky.social teams for their collaboration starting in 2022 during the Imaging Latinoamérica workshop.

Check out this new approach to colocalization analysis from @owfpuls.bsky.social along with members of the AIC and Advanced Bioimaging Unit teams, now out in @jcellsci.bsky.social! journals.biologists.com/jcs/article/...

Looking a little closer with the help of the @aicjanelia.bsky.social we used iPALM to see where septins were in relation to the actin. While they colocalize in the X-Y plane, septins are actually consistently below the actin stress fibers, closer to the membrane.

Getting started with analysis can be daunting, especially given the many tools available. In our latest @focalplane.bsky.social post, @scientistrachel.bsky.social & Owen Puls discuss framing analysis questions to extract informative biological information. focalplane.biologists.com/2025/12/15/h...

In a new post from @aicjanelia.bsky.social and @anjanja1024.bsky.social, Kevin Hamill shares ‘a day in the life’ of an AIC visitor. Kevin’s team went to the AIC to image components of the basal membrane using iPALM. focalplane.biologists.com/2025/11/24/y...

We are kicking off a blog series with @focalplane.bsky.social to provide insight into life at the AIC, microscopy careers, image analysis, and more! Read our intro post here: focalplane.biologists.com/2025/11/20/a...

📢Did you know that researchers from around the world can apply for access to the latest microscopy technology & expert advice @aicjanelia.bsky.social? In this blog series, the AIC team will give us an insight into how the Center works & their global impact. focalplane.biologists.com/2025/11/20/a...

I learned so much from the @aicjanelia.bsky.social team at the #InsightFromImaging 2025 workshop this past week. Thank you so much! It was so fun and informative. I enjoyed the discussions between #HannaGrayFellows and the AIC team at @hhmijanelia.bsky.social. I’m off to do bioimaging experiments! 🔬

One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬

For our final lecture at #InsightFromImaging 2025, @jesseaic.bsky.social is discussing electron microscopy and correlative approaches, including results from our FIB-SEM system, which can achieve 4 nm resolution in 3D!

We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth. Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE

Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.

This morning at #InsightFromImaging we focused on particle tracking. After a lecture by @jesseaic.bsky.social, participants split into groups to use #TrackMate to track our HL60 migration data from yesterday, followed by presentations on their results 📈

Yes, they are the same field of view 😄 Bonus additional #InsightFromImaging cell from a different FOV here:

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.

Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.

For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?

I had a wonderful time discussing image analysis with HHMI #HannaGrayFellows this morning. I look forward to continuing the chat throughout #InsightFromImaging this week.

This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches

We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬

When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃

For #SpookyMicroscopyFriday I present the mugshot of the head of the zebrafish mafia

Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.

Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬

Today we're wrapping up a flatworm imaging project on our SiMView microscope. We've enjoyed having @kinglhung.bsky.social and team from the lab of @ardemp.bskyverified.social here and look forward to working with them on analyzing their images!

Today we're wrapping up 3 weeks of zebrafish imaging on our MOSAIC system. We've enjoyed having Lisa and Miray of @annamolofskylab.bsky.social here and wish them safe travels home!

Today we're wrapping up an iPALM visit with @anirban-banerjee.bsky.social, @souravsgw.bsky.social, and team. Wishing them safe travels home!

We've enjoyed having Marise and Samara from the lab of @melaniedwhite.bsky.social here to image quail embryos on our MOSAIC system. We wish them safe travels home and happy data analyzing!

We are wrapping up a project imaging cancer cell spheroids on our SiMView system today. We wish Elizabeta Belova of @temple.edu happy data analyzing and safe travels home!

Today was a super fun first day of imaging @aicjanelia.bsky.social with @vanderspuy.bsky.social and @samarar.bsky.social. All hail the quail!🧪🔬#DevBio

Today we're wrapping up a dicty project on our MOSAIC system. We've enjoyed having Yiyan and Saki of the Devreotes lab here and wish them happy data analyzing!

My first SiMView project and it was such a fun one. Especially working with my favorite organism :) there is never anything wrong about staring at developing Drosophila in 4D!

We're wrapping up a fun three weeks of drosophila imaging on our SiMView microscope with the Mavrakis lab from @institutfresnel.bsky.social Wishing their team safe travels home and happy data analyzing!

We've reached the end of another full week at the AIC! Esrah Du from the Catteruccia lab is working on FIB-SEM segmentation, and we've enjoyed having Caterina Di Ciano-Oliveira of Unity Health Toronto here for job shadowing with our team.

Deadline is fast approaching!

Our deadline is coming up soon :)

Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).

Less than one month left to apply for our current call for proposals (deadline June 16)! Reach out to our team for a free technical consultation before proposal submission. Learn more at www.aicjanelia.org/apply 🔬

Today we are wrapping up a MOSAIC project imaging Drosophila with @blankenshiplab.bsky.social Wishing them safe travel home and happy data analyzing!

Last week we wrapped up an iPALM project with Shannon from the @mrobichaux.bsky.social lab at #WVU. Wishing her luck with her data analysis!

Back MOSAICing @hhmijanelia.bsky.social... as always a great imaging experience, and they have an open call for apps: www.aicjanelia.org/apply @aicjanelia.bsky.social

We’ve had an incredible experience being hosted at the AIC at Janelia for iPALM imaging. Tremendous support and expertise. If your project could benefit from advanced imaging, check out their instruments (FIB-SEM, iPALM, LLSM and others) and get in touch.👇

A new call for proposals is open at our Advanced Imaging Center for imaging on our pre-commercial microscopes. Reach out for a technical consultation and learn how to apply to use the newest technology for free!

Applications are open for using @aicjanelia.bsky.social microscopes! Reach out to our team for a free technical consultation to get feedback on your proposal before submission.

A new call for proposals just opened with @aicjanelia.bsky.social . Do not miss this unique opportunity to collaborate with microscopy experts to advance your project!

The latest AIC Call for Proposals is now open! Deadline is June 16, 2025. Come use @hhmijanelia.bsky.social imaging technology and expertise for FREE. Visit www.aicjanelia.org/apply for details. Before applying, contact us to schedule a technical consultation!

Today we're wrapping up an iPALM project with John Henson (Dickinson College) and Brad Shuster (@nmsu.edu). We've enjoyed having them (and their sea urchins) here at Janelia, and wish them safe travels home!

This week we're wrapping up two AIC visits at once! @kylejacobs.bsky.social from UCSF was here for a project on our iPALM system.