My first post here, we have a new preprint out from @aicjanelia.bsky.social! Upright LSFM is fantastic for live imaging, but not amenable to all samples - especially those requiring an air-liquid interface. We worked with Tokai Hit to try to fix that. www.biorxiv.org/content/10.6...

Check out this new approach to colocalization analysis from @owfpuls.bsky.social along with members of the AIC and Advanced Bioimaging Unit teams, now out in @jcellsci.bsky.social! journals.biologists.com/jcs/article/...

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

Looking a little closer with the help of the @aicjanelia.bsky.social we used iPALM to see where septins were in relation to the actin. While they colocalize in the X-Y plane, septins are actually consistently below the actin stress fibers, closer to the membrane.

Starting with your biological question can help focus your #bioimageAnalysis goals. Learn more in the latest @aicjanelia.bsky.social post on @focalplane.bsky.social 👇

In a new post from @aicjanelia.bsky.social and @anjanja1024.bsky.social, Kevin Hamill shares ‘a day in the life’ of an AIC visitor. Kevin’s team went to the AIC to image components of the basal membrane using iPALM. focalplane.biologists.com/2025/11/24/y...

And, if you've ever wondered what it is like to be a visitor to the AIC, check out this post by @anjanja1024.bsky.social and Kevin Hamill about visiting to use our iPALM system: focalplane.biologists.com/2025/11/24/y...

We are kicking off a blog series with @focalplane.bsky.social to provide insight into life at the AIC, microscopy careers, image analysis, and more! Read our intro post here: focalplane.biologists.com/2025/11/20/a...

We moved the AI@MBL course "Deep Learning for Microscopy Image Analysis" to HHMI Janelia (@hhmijanelia.bsky.social). Join us for two weeks of intense lectures, exercise, and hands-on project work! Course dates: June 4-18 2026 Application by: January 15 2026 www.janelia.org/you-janelia/...

I learned so much from the @aicjanelia.bsky.social team at the #InsightFromImaging 2025 workshop this past week. Thank you so much! It was so fun and informative. I enjoyed the discussions between #HannaGrayFellows and the AIC team at @hhmijanelia.bsky.social. I’m off to do bioimaging experiments! 🔬

One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬

We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth. Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE

Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.

Working with #TrackMate, participants at #InsightFromImaging were able to explore tracking parameters and the tradeoffs inherent in analysis. It was another fun discussion! 🤓

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.

Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.

For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?

I had a wonderful time discussing image analysis with HHMI #HannaGrayFellows this morning. I look forward to continuing the chat throughout #InsightFromImaging this week.

We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬

When #halloween meets #FluorescenceFriday 🎃

Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.

Hippocampus masquerading as a 🦇 for Halloween 🎃 #FluorescenceFriday Image credit to the @lieberinstitute.bsky.social Microscopy and Spatial Biology Core 🧠🔬🧪

When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃

A halloween cartoon for @newscientist.com

I'm excited to be co-organizing #InsightFromImaging 2025! We have many interactive activities planned, including hands-on image analysis labs 🤓

Today marks 4 years of enjoying the beautiful @hhmijanelia.bsky.social campus 🌳

Today we're wrapping up a flatworm imaging project on our SiMView microscope. We've enjoyed having @kinglhung.bsky.social and team from the lab of @ardemp.bskyverified.social here and look forward to working with them on analyzing their images!

Who does microscopy and has never heard of Bio-Formats? You probably used it anyway

We've enjoyed having Marise and Samara from the lab of @melaniedwhite.bsky.social here to image quail embryos on our MOSAIC system. We wish them safe travels home and happy data analyzing!

Google search's AI summary is like the loud confident idiot in the room who doesn't always know the answer to your question but HAS TO be the first one to reply to it.

The Confocal Room

Today was a super fun first day of imaging @aicjanelia.bsky.social with @vanderspuy.bsky.social and @samarar.bsky.social. All hail the quail!🧪🔬#DevBio

We are wrapping up a project imaging cancer cell spheroids on our SiMView system today. We wish Elizabeta Belova of @temple.edu happy data analyzing and safe travels home!

Today we're wrapping up a dicty project on our MOSAIC system. We've enjoyed having Yiyan and Saki of the Devreotes lab here and wish them happy data analyzing!

We're wrapping up a fun three weeks of drosophila imaging on our SiMView microscope with the Mavrakis lab from @institutfresnel.bsky.social Wishing their team safe travels home and happy data analyzing!

Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).

Back MOSAICing @hhmijanelia.bsky.social... as always a great imaging experience, and they have an open call for apps: www.aicjanelia.org/apply @aicjanelia.bsky.social

Last week we wrapped up an iPALM project with Shannon from the @mrobichaux.bsky.social lab at #WVU. Wishing her luck with her data analysis!

April is #CitizenScienceMonth! There is still time to join @scistarter.bsky.social and be part of #OneMillionActsofScience 2025. I had fun observing some trees for the Globe Observer program 🌳

We’ve had an incredible experience being hosted at the AIC at Janelia for iPALM imaging. Tremendous support and expertise. If your project could benefit from advanced imaging, check out their instruments (FIB-SEM, iPALM, LLSM and others) and get in touch.👇

Good advice! 👇

A new call for proposals is open at our Advanced Imaging Center for imaging on our pre-commercial microscopes. Reach out for a technical consultation and learn how to apply to use the newest technology for free!

Applications are open for using @aicjanelia.bsky.social microscopes! Reach out to our team for a free technical consultation to get feedback on your proposal before submission.

Today we're wrapping up an iPALM project with John Henson (Dickinson College) and Brad Shuster (@nmsu.edu). We've enjoyed having them (and their sea urchins) here at Janelia, and wish them safe travels home!

And the Nejsum Lab (nejsumlab.au.dk) from @au.dk is wrapping imaging our on our MOSAIC system. We wish both groups safe travels home!

This week we're wrapping up two AIC visits at once! @kylejacobs.bsky.social from UCSF was here for a project on our iPALM system.

🧪Job opening at Janelia @hhmijanelia.bsky.social Integrative Imaging platform for a bioimage analyst. Please repost.

Opportunity to join Integrative Imaging and work with the AIC team on #bioimageanalysis! 👇

This is an #opportunity to join Integrative Imaging and work with the @aicjanelia.bsky.social team! I enjoy getting to help researchers design and implement #bioimageanalysis approaches for a wide variety of #microscopy projects in this role 🤓