One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬

For our final lecture at #InsightFromImaging 2025, @jesseaic.bsky.social is discussing electron microscopy and correlative approaches, including results from our FIB-SEM system, which can achieve 4 nm resolution in 3D!

We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth. Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE

Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.

This morning at #InsightFromImaging we focused on particle tracking. After a lecture by @jesseaic.bsky.social, participants split into groups to use #TrackMate to track our HL60 migration data from yesterday, followed by presentations on their results 📈

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.

Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.

For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?

This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches

We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬

When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃

Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.

Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬

That was challenging and big fun! Safe travels home!

Today we're wrapping up 3 weeks of zebrafish imaging on our MOSAIC system. We've enjoyed having Lisa and Miray of @annamolofskylab.bsky.social here and wish them safe travels home!

The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

Another three imaging-packed weeks :) it was a pleasure working with you.

My first SiMView project and it was such a fun one. Especially working with my favorite organism :) there is never anything wrong about staring at developing Drosophila in 4D!

Our deadline is coming up soon :)

Less than one month left to apply for our current call for proposals (deadline June 16)! Reach out to our team for a free technical consultation before proposal submission. Learn more at www.aicjanelia.org/apply 🔬

Today we are wrapping up a MOSAIC project imaging Drosophila with @blankenshiplab.bsky.social Wishing them safe travel home and happy data analyzing!

We’ve had an incredible experience being hosted at the AIC at Janelia for iPALM imaging. Tremendous support and expertise. If your project could benefit from advanced imaging, check out their instruments (FIB-SEM, iPALM, LLSM and others) and get in touch.👇

A new call for proposals is open at our Advanced Imaging Center for imaging on our pre-commercial microscopes. Reach out for a technical consultation and learn how to apply to use the newest technology for free!

We're looking for 1-2 enthusiastic early career chemists to join our Open Chemistry team at @hhmijanelia.bsky.social. Come help us create amazing dyes and share them with the world. Please share with the best recent/soon-to-be B.S. chemists you know! bit.ly/42w8C6N

That was some fun imaging!

Come and work with us. We're really fun :)

We’ll be continuing to award JCS-FocalPlane Training Grants in 2025. They are open to ECRs that otherwise wouldn’t have sufficient funding to attend a microscopy or bioimage analysis course to support their research in cell biology. www.biologists.com/grants/jcs-f... @jcellsci.bsky.social

Many thanks to the @hhmijanelia.bsky.social Lab Coordinators for another wonderful Cookie Party! The @aicjanelia.bsky.social / @i2janelia.bsky.social team had a ton of fun! Photo featuring: @desantis42.bsky.social, @mairahmoon.bsky.social, @harikrushnan.bsky.social, @anjanja1024.bsky.social

Non-native English speakers need 50% more time to write a paper When they do, they face a 2.5 times higher chance of being rejected because of language journals.plos.org/plosbiology/...

Members of the Integrative Imaging team at @hhmijanelia.bsky.social work on all aspects of a microscopy project -- from sample preparation to image acquisition to data visualization and quantification. Meet the team with this starter pack: go.bsky.app/BEZ46y4

My first visitors! It was a joy :)

Hello Bluesky! The AIC makes HHMI Janelia imaging technologies and expertise available to the world at no cost. Learn more about our instruments and programs at our website: www.aicjanelia.org

Sharing this very nice, multi-part, blog by a current visitor to the Advanced Imaging Center @aicjanelia.bsky.social at HHMI Janelia Research Campus👇. Our next proposal call to this unique open-access microscopy program will be announced in early 2025. lantsandlaminins.com/2024/11/04/y...