Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

🎸Why this is exciting!!🦖 Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets. This opens HUGE opportunities to count in tissues, organisms, or clinical samples!

We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ! We measured the abundances for Complex I, III, and IV. How they differ in absolute abundances in metabolically distinct subpopulations And even how T-cell activation changes supercomplex density

How does qExM work? We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples. The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!

We validated qExM first on nuclear pore complexes, attempting to quantify the 8 fold symmetry without using this prior knowledge. We achieved a mean error of just 9.4% 😍🔬🎸 With these tools in place we moved to tackle protein complex abundances that were unknown....

🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology

#Mitochondria or Alien script? 🧪🔬👽

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

Accidentally festive #Celegans gonad while trying out some awesome LUTs by @kwolbachia.bsky.social for #FluorescenceFriday 🧪🔬

The PIs of Tomorrow session is part of the LS² Annual Meeting 2026. We have now chosen our 4 finalists: Theodora Constantin Juan Cruz Landoni Olimpia Bompadre Alaz Özcan More info: annual-meeting.ls2.ch/2026/special... Join our AM to see their talks: annual-meeting.ls2.ch/2026/registr...

🔬 Curious about #mitochondria #imaging but unsure where to begin? Our review might help! 🚀📜🧪 www.annualreviews.org/content/jour... #Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA

#StarterPack for the #Mitochondria research community! Very much a work in progress, comment below/reach out to be added! #Mito #Mitochondriac go.bsky.app/J6Bu5fL

Co-led with @jclandoni.bsky.social and fantastic support from Keaton Holt, Elena Koslover, @tatjanakleele.bsky.social , @sulianamanley.bsky.social , Sheda Ben Nejma, and Berna Durmus, you can find the preprint here: www.biorxiv.org/content/10.6... It's wonderful to see this project out in the wild

Using photoconversion, volumetric imaging, mitochondrial segmentation, and network simulations, we identified a distinct, proliferative mitochondrial subpopulation positioned around the nucleus. From there, single mitochondria scatter new material across the cell, maintaining network in balance

🔬🥟 What do empanadas and #mitochondria have in common? More than you'd think. Our "empanada-shaped" cells just helped uncover hidden subpopulations of mitochondria in your everyday, unpolarized cell lines. Curious how mitochondrial trafficking shapes network health?

Preprint alert 🚨 Ever wondered what role fast #mitochondria trafficking plays in the simple cells we mostly use? In @julius-w.bsky.social et al. we use mitodynamics + simulations to identify the importance of outwards transport to distribute new material and keep network balance! Link below!

Tune in! 🔬

At this week's #VirtualPub, we welcome Juan Cruz Landoni, EPFL, on "Pearling drives mitochondrial DNA nucleoid homeostasis." 🗓️ Friday, Dec 12 @ 13:00 CET Register here🔽 www.eurobioimaging.eu/events/pearl...

#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software ✅ Free 🔓 Open Source 🧬 More cloning methods than SnapGene 🤖 Can be automated with python 👨‍🔬 Built by a researcher — for researchers! 👉 Check it out at opencloning.org

It's not #FluorescenceFriday but unexpectedly beautiful data should always be celebrated 💚 #PKMO -stained #mitochondria cristae in #STED

Another year and another group of brilliant young students preparing for the International Biology Olympiad #IBO 💪☘️ Thank you for making teaching so fun and inspiring, bon courage à tous, and most importantly: enjoy it!

Smartest booth luring strategy I've seen: a pro barista on site 🤯 Drop the free pens. Science runs on espresso ☕️

That's a wrap on a much-needed family vacay, thank you Costa Rica for your incredible nature 🌋🦥🏞️🐠🐡🐒🦈, now back to our regularly scheduled programming 🔬

#Writing with a view 🤩

New paper - MAPL strikes again! Interested in mitochondrial signalling, inflammation, lysosome biology, pyroptosis, and Parkinson's disease? Have a look, there's something for everyone! Feeling grateful! @mitocollier.bsky.social Funded by #CIHR, @asapresearch.parkinsonsroadmap.org. rdcu.be/eKKz1 🇨🇦

What complex are you if you have a very versatile company in close proximity? The MICOS Complex. We present the MINDNet: Proximity Proteomics revealing the nanodomain interactome in the mitochondrial inner membrane, done by the talented Yulia and team.

Still buzzing from the incomparable @embl.org #SeeingisBelieving conference! 🔬 Great science and truly amazing people, thanks everyone! #EESImaging

Who's coming next week? 🤩🔬 #EESImaging #SeeingisBelieving

How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇

From MBoC: Wallace F. Marshall (UCSF) and team profile the giant ciliate Stentor pyriformis—symbiotic algae in microtubule baskets, symbiont-dependent phototaxis, and a standard genetic code. Paper: www.molbiolcell.org/doi/10.1091/... #CellBiology

"Who needs a 30x zoom anyway?" I just found out the answer 🌙🤩

Proof that I also care about other organelles 😁🔬 🟢 #ER 👉👈 #Mitochondria 🟣

Trying my hand at HTML/CSS using @github.com hosting for a new personal website 💻 Highly recommend! jclandoni.github.io Comments/tips welcome (should look nicer on desktop but mobile should work too).

Still not a bean 🫘 #Mitochondria (and friends) in #retina cells 👀 EM tomography 🔬🧪

So excited to follow the opening symposium of #SWAN 🦢[Sustainable Wellbeing Across Lifespan] at @helsinki.fi today! Congrats to @awartiovaara.bsky.social and all members for the exciting ongoing and future reseach! #mitochondria #metabolism #aging #health

Order, chaos & complexity #Mitochondria in #muscle cells 💪🔬, color-coded by 3D depth 🧪

🐟 Zebrafish (Danio rerio): Clear as glass, zebrafish embryos let you watch organs form in real time. A powerhouse for studying organogenesis and regeneration, they’re also champions of transgenic expression of fluorescent proteins 🌈 image from Gopi Shah 🧪 #ModelMonday #DevBio

In case you haven't heard, #Argentinian scientists are live-streaming their deep-sea mission with real-time interactive commentary. It's genius, mesmerising, and triggering huge public interest and support for #science funding 🐙🧪 And the memes are great! #CONICET #MarDelPlataCanyon

Here it is❗️Our updated inventory of genes required for #OXPHOS & findings on #mito defects in #Menieres is now out at Cell Reports by Cell Press! 🎉   Thank you to all of our collaborators and funding sources, this was a great and exciting project from start to finish. 💫 www.cell.com/cell-reports...

Our updated inventory of genes required for #OXPHOS & findings on #mito defects in #Menieres is now out at Cell Reports! 🎉 🧫 New galactose #CRISPR screen 🎯 481 genes required for #OXPHOS ⚙️ #FAM136A in IMS proteostasis 👂 Mito defects in #Menieres www.cell.com/cell-reports...

Congrats to PhD student @marcellharhai.bsky.social , who joined the lab 2y ago (!) and led this work, to second author M. Foged, and to all our collaborators! We are thankful to our funding sources including the @FSRMM, Novartis and @snsf.ch. @dib-unil.bsky.social @fbm-unil.bsky.social

Join us in Engelberg for an exciting #EMBO workshop on #mitochondria in the nervous system #EMBOmitoNervousSys. Great speaker line up and opportunities for short talks from early career scientists! Submit your abstract until May 30st! meetings.embo.org/event/25-mit...

Is there still a reason for dumping figures at the end of manuscripts instead of in-text, even for #BioRxiv preprints? My scroll-finger is exhausted... 🙏🧪📜

Want to capture elusive events without stressing out your cells? Check out @wl-stepp.bsky.social hybrid-EDA! A smart microscopy approach to detect events in phase, and only image when it matters! 🔬🧫🧪 www.biorxiv.org/content/10.1... @sulianamanley.bsky.social @maweigert.bsky.social

A key insight was building temporal information and memory into the ANN architecture. A huge thanks to all, especially our ‘smart’ (in so many ways) partner @maweigert.bsky. We could now detect local depolarization following constriction, using commonly used (bleachy TMRE) fluorescent biosensors.

Our latest preprint www.biorxiv.org/content/10.1..., describes Willi Stepp’s project to make smart microscopy even gentler by doing event detection in phase contrast. We developed neural networks to detect mito-LD and mito-lysosome contacts, as well as mitochondrial pre-fission constrictions.

Combining bacterial end joining with programmable nucleases, we induced mtDNA deletions in human cells at varying heteroplasmy and examined their metabolic and cellular effects. Simple tool to apply across any cell type and engineer pathogenic mtDNA deletions. www.sciencedirect.com/science/arti...

The magic of the live #mito straight from the microscope 🔬 for #FluorescenceFriday - brought to you by #SYBRgold 🟢 and #PKMO 🟠 @spirochrome.com @carolinklose.bsky.social @lycasworks.bsky.social

Fresh new paper alert 🚨 by @marcellharhai.bsky.social @jourdainlab.bsky.social et al. expanding the inventory of potential #mitodisease genes and a herculean deep-dive into FAM136A across models and human disease! 🧪🧫🐭👂 Really happy to see it out and to have played a small part 🔬 #mito #sciencesky

First PhD manuscript preprint OUT NOW! ‼️ 🧫 New galactose #CRISPR screening strategy 📝 Updated OXPHOS-regulating gene inventory 🧬 #FAM136A in the IMS 👂 Mito defects in #Ménière’s disease All come together in our latest work available at www.biorxiv.org/content/10.1...