Another outcome from the Appose processing hackathon: Cellpose simple tile processing in Fiji, with #imglib2 and #appose only. With @gaellel.bsky.social and @strigaud.bsky.social

Got to play around with Holotomographic imaging of muscle cells yesterday via the @uclastemcell.bsky.social imaging core and the results are pretty spectacular 🤩 You can see everything from mitochrondria to lamellipodia to macropinocytosis…with no labels!

Tiny structures, bright insights — happy #FluorescenceFriday! 🔬

For this #FluorescenceFriday, some endosomes imaged with MI-SIM. Green: Rab21, Magenta: F-actin, Cyan: EPLINalpha.

Happy #FluorescenceFriday. I found this embryonic macrophage contacting a non-myeloid cell (bottom right corner) and then moving away. What's cool is that this contact is strong enough to slightly bend the cell and you can just about to see it. Forces at the cellular scale is so curious! 😁

Mechanical forces & cell shape guide how plant stomata form🥬👄 @leoserra.bsky.social & Euan Smithers from @robinsonsci.bsky.social's team uncover how the interplay between cell shape & mechanical stress influences the orientation of stomata doi.org/10.1016/j.ce... www.slcu.cam.ac.uk/news/mechani...

introducing barfbow, a little tool that aims to encourage the use of variable brightness to improve effective com, accessibility and scientific accuracy in figures. github.com/evolvwing/ba... If you need palettes with colors that are easy to distinguish on a plot or map, pls check it out!

Happy #FluorescenceFriday! I captured this shot of a group of embryonic macrophages expressing LifeAct and H2B migrating inside tissue. These cells can use lamellipodia, filopodia, or blebs to migrate, and are frequently switching between these different modes or combining them. So fascinating.

Have a nice #FluorescenceFriday This is an insect larvae with endosymbiotic bacteria enverywhere! (in orange) #Microscopy #Sciart

Indirect immunostaining of HeLa cells with Fibronectin 🟣 and alpha-Tubulin 🟢. DAPI 🔵 #FluorescenceFriday

Happy #FluorescenceFriday. I feel so lucky to get to work with these weird and curious primary macrophages from 🐸. They exhibit myriad different morphologies and behaviours, including spinning endlessly and stationary, kinda like progenitor cells under compression from 🐟. Also actomyosin is strong!

Happy #MicroscopyMonday! Confocal image of a Schistosoma mansoni miracidium larva with alpha-tubulin stained cilia in green and nuclei in magenta. Imaged by Sarah Kerr and Karel Mocaer in the Nikon Imaging Center at Heidelberg University.

A special #FluorescenceFriday. Our first in situ from Bath!! Cora did an amazing job visualising expression of the BEST4 cell marker cftr in magenta and the progenitor cell marker her15.1 in cyan in this villus from an adult fish gut.

#microtubules in orange hot, #F-actin greyscale, Fibroblast, spinning disc. @marcoenriquez18.bsky.social @aibnatuq.bsky.social #fluorescenceFriday

Tadpole gut contents. I just think it looks neat.

The high-speed logistics of a lung cancer cell. Watch GFP-Rab25 (Gold) move cargo through the internal recycling pathway of A549 cells. Static maps are great, but time-lapse shows the real traffic. Want your science/tech to be highlighted? DM me at Viroscope!

Our preprint on Antarctic fish cell cultures is out now! Loads of pretty nice super-resolution images. Read it here: www.biorxiv.org/content/10.6...

This is a section of juvenile moss (Physcomitrium patens) tissue, called protonemata. The apical cells expand by tip-growth, while subapical cells sometimes divide to make lateral filaments. Cytosolic GFP is in green, and chlorophyll autofluorescence is in magenta. #microscopymonday #moss

🧬This #WorldDNADay, we spotlight this breathtaking capture by @christlet.bsky.social#WorldDNADay 🔬With the NSPARC detector, Christophe reveals the intricate architecture of Rat2-cells 📝This is part of a broader story on advances in super‑resolution microscopy. Read the article here: https://bit.ly/3OINgjB

When science meets art, it’s #FluorescenceFriday. 🔬

🧠 Layers on layers of neural architecture Stacked confocal view of columnar interneurons in the developing fly brain 🟢 GFP-lit neurons revealing precise organization 📸 #FluorescenceFriday by Rajesh Raghuvanshi Submit your images here ➡️ @raghu5.bsky.social docs.google.com/forms/d/e/1F...

Happy #FluorescenceFriday! Just some random data I collected from a while back, actin and myosin in a U2OS cell imaged on our lattice light sheet microscope.

New Pre-Print Alert! Evolving initial conditions: an alternative developmental route to morphological diversity with Shannon Taylor and @jamesehammond.bsky.social www.biorxiv.org/content/10.6...

The Landoni Lab opens this summer at UNIL! @fbm-unil.bsky.social 🔬 Quantitative #imaging + systems biology to decipher how #metabolic architecture & #mtDNA dynamically shape tissue development, #aging and #mitodisease. Beyond excited to fire up the lasers, open positions soon! 🧪 #mitochondria #newPI

#TubulinTuesday!

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

There's an update of #EZreverse, it is much quicker and supports drag-and-drop joachimgoedhart.github.io/EZreverse.html

Sharing my latest preprint as a re-introduction to bsky! biorxiv.org/content/10.1... ---- Everyday, cells like this crawl through your body to protect you. While we know that their front 🔴 and back 🔵 polarity programs work together, how exactly do these two processes communicate?

It's funky axons time 🔬 🧪

🔬 #ImageOfTheWeek powered by the #PelletierLab Stunning imaging from recent work of Philip et al. (2025) in #EMBOJournal: tinyurl.com/3p44bh9y Thanks to the team at Lunenfeld-Tanenbaum Research Institute @sinaihealth.bsky.social, #Nikon CoE & NBCC Imaging Facility #FluorescenceFriday #V3UpgradeToSIM

It's #FluorescenceFriday and these retinal astrocytes understood the assignment: branching, glowing, and casually laying down the blueprint for the retinal vasculature #Retina #ConfocalMicroscopy

🪱 Serving head-to-tail fluorescence 💅 Whole-animal FISH in the planaria Schmidtea mediterranea: 🔵 Head 🟣 Gut 🟡 Tail ✨ Masters of regeneration and color coordination 📸 Image from Viraj Doddihal #FluorescenceFriday To be featured submit your images here ➡️ forms.gle/PWyaViekkNNn...

Mammalian membranes are thought to universally avoid macroscopic solidification, as reduced fluidity impairs plasticity and protein function. Cells counter this via lipid unsaturation and cholesterol buffering. However, the ER is deliberately cholesterol-poor to maintain high-curvature membranes!/5

#FluorescenceFriday - experiments take so long (especially for PIs that moonlight as postdocs), between breeding, tamoxifen, waiting for the 🐭 to grow up, slicing, clearing, staining for CD206 then finding a chunk of time on the 🔬...but then I get to image all my fav cells & it's SO worth it. ❤️

✨ This Xenopus 🐸 tadpole came to #FluorescenceFriday serving eye-conic energy 👀 Stage 39 eye stained with a whole rainbow: 💙 DAPI | ❤️ Acetylated tubulin | 💚 RPE | 🩵 Rhodopsin | 💜 Pax6. 📸 Image from Jian Sun.

Going through some old microscopy data, and I have to say - I maintain that #mitochondria are the most beautiful organelles, but the #ER might be a very close second. #FluorescenceFriday Live HeLa cells, ER imaged by STED, mitochondria with confocal.

Cellular Liquid Gold 🔬 This time-lapse shows an A549 cell over-expressing Sec61B, outlining the Endoplasmic Reticulum. Imaged at 1 frame per 500ms, it reveals just how violently active our cellular infrastructure really is! What is your favorite organelle to image? 👇 #SciArt #FluorescenceFriday

Copy paste into the address bar of your browser for a random generative artwork. No AI involved.

Striking microtubule-actin compartmentalization. A cell so big must organize things I guess!

I'm so pleased to see this work on neural crest development out in Nature today! This was a truly fantastic collaboration with @gleesonlab.bsky.social, Keng loi (Harry) Vong and @xiaoxuyang.bsky.social. Huge thanks to everyone involved 👏 🧪🐣 #devbio www.nature.com/articles/s41...

Oligodendrocyte precursor cell in the spinal cord of a transgenic zebrafish larva. Credit to @jiaxingli.bsky.social. #ZebrafishZunday 🧪

Chromosomes stuck behind the spindle are ticking time bombs for aneuploidy. We uncovered a mechanical rescue mechanism where microtubule pivoting repositions these high-risk chromosomes! Out last week in @natcomms.nature.com, here's a thread 🧵 shorturl.at/OD89x

dissecting & combating #CNS #scarring

Reactive microglia (🟠) and astrocytes (⚪️) as well as Aβ plaques (🔵) in the hippocampal dentate gyrus of a 5xFAD mouse model of Alzheimer's disease. #FluorescenceFriday

🥚 Egg hunt but make it neuroscience & microscopy for #FluorescenceFriday !💐 With Easter around the corner, I'm sharing some of my latest imaging & sciart work: mouse olfactory bulbs made to look like beautiful Fabergé eggs! 👑 The images were taken from 3D datasets of whole cleared mouse brains.

Fluorescence Friday - Massage edition ! 🤏 By @pierre-bercier.bsky.social #CellBiology #Microscopy #Nucleus #FluorescenceFriday @crg.eu @prbb.org

✨ Somite symmetry never fails to mesmerize 🐟 Trunk somites of a 1-day-old zebrafish embryo with nuclear (💙) and membrane (🧡) labelling 📸 Image credit: Matyas Bubna-Litic (Mongera lab, UCL) 🔬 #FluorescenceFriday

#Cilia are beautiful! That's it, that's the tweet. Oh, and Happy #FluorescenceFriday 🔬