#Mitochondria or Alien script? 🧪🔬👽

Accidentally festive #Celegans gonad while trying out some awesome LUTs by @kwolbachia.bsky.social for #FluorescenceFriday 🧪🔬

Glowing proof that discovery can be beautiful — #FluorescenceFriday! 🔬

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

*New preprint alert* uncovering a mechanical pacemaker that synchronizes nephron formation with branching of the kidney's epithelial tubule tree. Read below to learn about this twisty journey lead by Sam Grindel and Sachin Davis in the lab. [Movie by Nils Lindstrom] www.biorxiv.org/content/10.1...

This movie shows lysosomes (orange) and keratin (gray) in a cultured cell over 10 minutes.

A favorite recent image of some busy microglia, lovingly named the Rat King (yellow CD68, pink Iba1, blue DAPI) for my first #FluorescenceFriday

A glittery, festive #FluorescenceFriday today, spent playing with some fun samples 🪩🕺

Back in 2016, I spent some time centrifuging chambers containing a kinesin/microtubule -based active gel so that all the material ended up on one side of the chamber. Then recorded how long it took to expand.

Frog eye, frog eye. Can I have a confocal microscope to play on for 12 hrs a day again please? 👉👈🥺 🐸

Unsolicited remix of some cool #FluorescenceFriday images from Matthias Böddener 😁can't go wrong with the Noice LUTS!

The arbuscules of arbuscular mycorrhiza

#LivingArchitectures We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay. www.musee-orsay.fr/fr/agenda/ev... Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night. -> Two shows on the 24th and 25th of January.

Immunostaining of a mouse brain blood vessel with Collagen type 4 (cyan) and GFAP (orange) Nuclei have been visualized by DAPI staining (blue). #FluorescenceFriday #bloodbrainbarrier

This distal gill tip is giving full branchial art vibes for #FluorescenceFriday 🦓🐟 — who knew oxygen exchange could be this pretty. 🔴 kdrl:mCherry highlights the vasculature ⚪ fgf10b:nEOS marks the pillar cells #Zebrafish #DevBio #SciArt

New preprint! Do you like ocean waves? We found similar waves on bacterial colonies! We found that this collective behavior, known as rippling, is nothing but surface waves on an active nematic. @princeton.edu @mpipks.bsky.social @ub.edu @icreacommunity.bsky.social www.biorxiv.org/content/10.1...

Zebrafish tail depicting muscle and cell nuclei. Done at @cabd-upo-csic.bsky.social, in @gloriabreacalvo.bsky.social lab. #FluorescenceFriday, #zebrafish, #DevelopmentalBiology, #microscopy

Our M.Sc. PBioC (Physical Biology of Cells and Cell Interactions) students at @goetheuni.bsky.social had their first hands-on encounter with STED microscopy this week — and they captured some truly stunning super-resolution images. #FluorescenceFriday #Superresolution

For this week #FluorescenceFriday, we'll be treated with one of our favourite animal models 🐸 . Here is a Xenopus laevis embryo imaged with a Lightsheet microscope showing macrophage migration. Purple🟣 = ectoderm nuclei, Red🔴 = macrophages. 📹: Hoang Anh Le, postdoc in the Mayor lab, UCL.

I have more live imaging of actin in B cells from this week for this #FluorescenceFriday

So excited to share this as a new junior PI: My brand-new lab website! 🎉🪰🌀 www.bischofflab.com Please pass it on to young, motivated researchers looking for PhD positions 😊 And for the #FluorescenceFriday community: don’t miss the SciArt Gallery! #CellBio #DevBio #PhDjob #PhDposition #Science

Final result (better to do the rolling-ball background subtraction before merging, and merging using Max rather than blending)

So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth! #protistsonsky 🧵

I finally got my hands on a MRI! Didn't help my wrist but now I have some really cool image stacks to play with 🤩

I like this movie, but a friend of mine likes to complain about the obvious stitching artifacts. I'll try harder next time, Michael. Vimentin (orange) and ER (blue) in an overnight acquisition.

I gave a talk at Blender Conference yesterday, showing how biological volumes work, and how to visualize them in Blender 😋 youtu.be/WPajSWX730o?... #bcon25

Still posting cytoskeleton videos, it seems. Actin this time. Sample: Lifeact-eGFP in HeLa cells. Modality: Airyscan confocal Timestamp is mm:ss and the scale bar is 5 µm.

iPS cell-derived cardiac myocytes (heart muscle cells) typically beat about once per second, so I usually speed up the movies I post; otherwise, scrollers might miss the action. But every now and then, a cell looks like this in real time. #CellBiology

Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.

Happy #FluorescenceFriday. This beautiful video shows slice by slice section of a zebrafish embryo's head. The embryo is almost completely transparent, proving that zebrafish is an amazing model system for microscopy. Brightfield, red = actin, blue = DAPI 📹: Postdoc Matyas BL (@Mongera lab, UCL)

Finally, the first public release of #BigVolumeBrowser, so after teasers, you can try it yourself. For details, please check the announcement post (1/2) forum.image.sc/t/bigvolumeb...

I updated the Image Viewer and the wiki page with many small bug fixes and features! If you haven't tried it yet, now it's even better ;) #microscopy #ImageJ #FluorescenceFriday #microscopyMonday

This week's #FluorescenceFriday, we'll be treated with one of the classical models of #devbio, the neural crest cells. Here is a beautiful video of neural crest cells with GFP-tagged focal adhesion kinase (🔵) and LifeAct-RFP (🟣) migrating on a Fibronectin matrix. 📹: Adam Shellard

I guess it never gets old...bc we love #CellMigration ATP has many roles: energy currency, hydrotrope, and *danger signal* Immune cells are faster after ATP exposure, F-actin (cyan) goes to the rear for nitro boost! www.science.org/doi/full/10.... @focalplane.bsky.social #fluorescencefriday 🧪🔬

🔬🍀 A timelapse movie (unpublished data) from the lab. In the movie, the nucleus is moving towards the base of the polarized root hair cell.

During my postdoc, I looked at hundreds of images like this 🤩 These are two apical caulonemal cells from moss Physcomitrium patens stained with MDY64 (shown in shades of orange). The natural autofluorescence of chlorophyll is in cyan. #microscopymonday #moss #plantcells #plantmicroscopy

This image by MDI Bio Lab's Travis Carney is a #drosophila larval brain. Neural stem cells and neurons are marked, including axons that project into the brain. The flare in the center of each lobe is part of a learning and memory center in flies. ZEISS Microscopy#drosophila#microscopymonday 🧪 🤝

Happy #FluorescenceFriday from these primary astrocytes grown on glass slides and robotically stained on the Biocare Oncore Pro X! 🧠🔬🧪 Cyan = GFAP Royal Purple = ALDH1L1 Salmon = Nuclear Counterstain

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

Two neurons went into a bar...

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

‪ Congratulations @centriolelab.bsky.social @dudinlab.bsky.social and @gautamdey.bsky.social for your publication in @cp-cell.bsky.social: Charting the landscape of #cytoskeletal diversity in #microbial eukaryotes #Expansion #Microscopy @sciencesunige.bsky.social www.unige.ch/medias/en/20...

In the spirit 👻 of Freaky Friday 🎃, here is a beautiful yet eery video of zebrafish retina development showing horizontal cells (🔵) finding their way out of the crowded amacrine (🟠) layer to settle beneath the photoreceptors. Happy #Halloween! 🎥: PhD student Rae Wong from the @nordenlab.bsky.social

An iPSC heart muscle cell forming sarcomeres videoed through a microscope by Emma Koory for 20 h before it dies most dramatically. This was our first try on a new microscope, and Emma now has the imaging parameters dialed in so her cells do not go POP! Alpha-actinin-2 is shown. #CellBiology

I don’t always manage to get the cochlea out… But I love it when I do! #FluorescenceFriday

Happy (and spooky) #FluorescenceFriday Switching from frogs (which are underrated btw) to zebrafish has really made me appreciate transparent tissues! 🐟 28 hpf, ⚪ nuclei, 🔴 F-actin

For this #FluorescenceFriday I wanted to share this spooky eye from a squid embryo! 👁️🦑

When #halloween meets #FluorescenceFriday 🎃