Sakers, @caglaeroglu.bsky.social et al. report that astrocytic neuroligins are functionally diverse proteins with unique intracellular protein–protein interactions. In vivo, ubiquitination of NL2 by Nedd4l is critical for #astrocyte #morphogenesis. rupress.org/jcb/article/... #Neuroscience

Heterogeneity of late #endosome/#lysosomes shown by multiplexed DNA-PAINT imaging. Quantitative pipeline developed by Charles Bond, Melike Lakadamyali @melikel.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

Our June issue is here! rupress.org/jcb/issue/22... The cover shows a maximum intensity projection from a confocal image stack showing an angiogenic sprout emerging from an engineered microvessel. From @lakynm.bsky.social et al. of the Matthew Kutys lab: rupress.org/jcb/article/... #Cytoskeleton

Wu and Hegde annotate and analyze the topology of 4,863 human endoplasmic reticulum–inserted membrane proteins and their 20,546 transmembrane domains, showing that transmembrane domains inserted by different pathways can tolerate different biophysical properties. rupress.org/jcb/article/...

PINK1 controls RTN3L-mediated ER #autophagy by regulating peripheral tubule junctions. From Ravi Chidambaram, Kamal Kumar, Susan Ferro-Novick and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

Strength in numbers: Proteins travel as condensates to reinforce cell-cell junctions under stress. Jiarui Jiang @jiarui219.bsky.social and @krothphd.bsky.social @rothenberglab.bsky.social discuss new work by Zheng et al. (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/...

Publishing in JCB enables authors funded by National Institutes of Health (NIH), Howard Hughes Medical Institute (HHMI), and other funders an easy pathway to compliance. Learn more: 👉 rupress.org/pages/nih-hh...

Any1 is a #phospholipid scramblase involved in #endosome biogenesis. From Jieqiong Gao, Christian Ungermann and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

Arl8b inactivates the Rab11a recycling pathway to promote LAMP1 sorting and #lysosome biogenesis. New study from Priya Chouhan (@priyachouhan.bsky.social ), Yogita Phogat, Amit Tuli, Mahak Sharma and colleagues (Indian Institute of Science Education and Research Mohali): rupress.org/jcb/article/...

T-cell #mechanobiology: how molecular forces shape immune function. New review from Judith Zubia-Aranburu, Lingxiao Zhang, and Mingdong Dong @au.dk: rupress.org/jcb/article/... #Biophysics #Immunology #Tcells

Ever wondered why anaphase onset looks so rapid (but maybe not perfectly synchronous). As it turns out, separase is playing a small numbers game - at least in fission yeast. Thanks to Wendi Williams, @silkehauflab.bsky.social , and everybody involved.

Hayase, Xu, Bi et al. show that E-cadherin promotes bile canaliculus elongation via RhoA activation and oriented cell division, while N-cadherin maintains hepatic #polarity by attenuating RhoA, defining distinct roles of dual #cadherin expression in #hepatocytes. rupress.org/jcb/article/...

Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2. From Jing Ze Wu, Sergio Grinstein, Spencer Freeman @spencerfreeman.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

Ana1/CEP295 regulates centriolar doublet-to-triplet conversion during #spermatogenesis. New from Zhou Chen, Dongqing Li, Nan Pang, Xiaomin Fang, Hélène Rangone @geneticscam.bsky.social, Jingyan Fu (China Agricultural University) et al: rupress.org/jcb/article/... #Cytoskeleton #Cilia #Flagella

Shared #circadian synchronicity in a syncytium. Hannah L. Coveney, Yuxin Hong, and Priya Crosby @crosbylab.bsky.social @edinburgh-uni.bsky.social discuss new work from Wang et al. of the Dunlap lab (rupress.org/jcb/article/...), in Spotlight: rupress.org/jcb/article/... #Biophysics #Neurospora

“Thank you, JCB, for upholding such high standards!” — Julien Dumont. Share your breakthroughs with a global audience. Learn more. hubs.la/Q048tT1P0

Appalabhotla et al. (rupress.org/jcb/article/...) engineer a light-controlled version of PLC-γ1 & show that activating it in an illuminated region of the cell membrane drives directional migration. Jichao Sun & Mo Chen discuss the findings: rupress.org/jcb/article/... @coronin.bsky.social

Saishree S. Iyer and Anna Akhmanova @utrechtuniversity.bsky.social review the mechanisms controlling plus-end elongation of centriolar and ciliary #microtubules, revealing shared principles. rupress.org/jcb/article/... #Centrioles #Cilia #Cytoskeleton

Lysosomal TPC2 channels disrupt Ca²⁺ entry and dopaminergic function in models of LRRK2-#Parkinsons. From Martina Gregori, Anthony Schapira, Sean Sweeney, Sandip Patel and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

Excited to share our latest study " #Rab11a recycling pathway to promote #LAMP1 sorting and #lysosome biogenesis" published in @jcb.org doi.org/10.1083/jcb.... Grateful to the mentors Dr. Amit Tuli and Dr. Mahak Sharma for their guidance. I would like to thank Yogita and all the co-authors.

Core clock protein subcellular dynamics coordinate local and global #circadian control in syncytia. New study from Ziyan Wang, Jay C. Dunlap and colleagues (Geisel School of Medicine at Dartmouth): rupress.org/jcb/article/... #Microbiology #CellSignaling #Genetics #Trafficking #Neurospora

Light-based modulation of astrocytic calcium for regulation of organelle dynamics and #morphogenesis. New tool developed by Lan Yang, Mikael Björklund, Cong Yi, Shue Chen, and Shue Chen (Zhejiang University-University of Edinburgh Institute): rupress.org/jcb/article/... #neuroscience #astrocytes

Akayama, Shima, Nakamura et al. show that TFEB is regulated by at least two different regulatory mechanisms during lysosomal damage: ATG conjugation system–dependent/independent regulation. rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

@jiarui219.bsky.social and I really enjoyed reading this paper and having the opportunity to summarize the key findings! We couldn't possibly cover everything cool about the paper, so I encourage you to give it a deep dive yourself!

Spotlight: New work by Zheng et al. (rupress.org/jcb/article/...) elucidates how #adhesion proteins are rapidly moved along the cell edge to vertices in response to mechanical forces. Jiarui Jiang and @krothphd.bsky.social discuss the findings: rupress.org/jcb/article/... #Mechanobiology

TMEM175 does not function as a proton-selective ion channel to prevent lysosomal over-acidification/ From Erika Riederer, Dejian Ren and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

Chouhan, Phogat et al. of the Mahak Sharma lab report that the small GTP-binding protein Arl8b recruits the Rab11a GAP, TBC1D9B, to inactivate Rab11a-mediated recycling of newly synthesized LAMP1 and mediate its efficient sorting to #lysosomes. rupress.org/jcb/article/...

Judith Zubia-Aranburu, Lingxiao Zhang, and Mingdong Dong @au.dk review multiscale forces governing #Tcell function, their disruption in disease, and how #mechanobiology can improve #immunotherapy design. rupress.org/jcb/article/... #Biophysics #Immunology

Homocysteine disrupts lysosomal function by V-ATPase inhibition. From Yang Yang, Qianjin Kong, Chaolian Liu, Mei Yang, Chonglin Yang and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

PI(3)P regulates mitochondrial dynamics through EXC-5–dependent #actin remodeling. Sneha Hegde and Marc Germain @uqtr.bsky.social discuss work from Zhao et al. (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/... #Biochemistry #Organelles #Mitochondria #Lipids

Chromosome segregation synchrony in S. pombe is noise-limited and arises without positive feedback. New study from Wendi Williams, Julia Kamenz @kamenzlab.bsky.social, Silke Hauf @silkehauflab.bsky.social @virginiatech.bsky.social and colleagues: rupress.org/jcb/article/... #CellDivision #Mitosis

New 📽️ short video summary by Lan Yang and Zhi Hong (Zhejiang University-University of Edinburgh Institute) on their study (rupress.org/jcb/article/...), "Light-based modulation of astrocytic calcium for regulation of organelle dynamics and morphogenesis" youtu.be/4KtKZlyvBoI #astrocytes

Spotlight: Coveney, Hong, and Crosby @crosbylab.bsky.social highlight work from the Dunlap lab (rupress.org/jcb/article/...), which reveals how #circadian rhythms are coordinated in a syncytial fungus. rupress.org/jcb/article/... #Biophysics #Neurospora

Zhou Chen, Dongqing Li, Nan Pang, Xiaomin Fang et al. identify Ana1 as a regulator of centriolar doublet-to-triplet conversion and show that triplet microtubule integrity is crucial for sperm structure and male fertility. rupress.org/jcb/article/... #Centrioles #Cilia #Flagella #Spermatogenesis

Kim, @spencerfreeman.bsky.social et al describe a mechanism for lysosomes to sense and respond to acute increases in their #membrane tension by relieving hydrostatic pressure via the outward transport of major osmoticants rupress.org/jcb/article/... 📕 In #Lysosomes 2026: rupress.org/jcb/collecti...

This collection features recent advances related to #lysosomes, focusing on their maintenance, regulation, and relevance to human health and biology. Studies include how lysosomes are maintained by ion channels & how homocysteine inhibits lysosomal V-ATPase function. 👉 rupress.org/jcb/collecti...

Are you headed to #GRC #Myelin meeting in Italy next week, and interested in speaking with a JCB editor about publishing? Get in touch with our Executive Editor Tim Spencer, who will be attending! Email Tim to book a time: ✉️ rupress.org/jcb/pages/ed...

#Neutrophil serine #proteases degrade endothelial cortactin and promote extravasation. New study from Idaira M. Guerrero-Fonseca, Michael Schnoor (Cinvestav-IPN) and colleagues: rupress.org/jcb/article/... #Immunology #Migration #motility #Trafficking

Wang, Dunlap et al. perform high-res, long-term live-cell imaging in #Neurospora crassa to visualize #circadian clock proteins at endogenous levels, revealing how subcellular organization & internuclear communication coordinate timing in a multinucleated syncytium rupress.org/jcb/article/...

Lan Yang et al. develop a light-based tool to precisely modulate astrocytic Ca²⁺ signals via endogenous IP3Rs, revealing how local vs global Ca²⁺ patterns differentially control organelle transport and #astrocyte #morphogenesis. rupress.org/jcb/article/... #neuroscience #gliobiology #optogenetics

A deep dive into functional #ribosome specialization. New Perspective from Seohyun Park, Fiona Fitzgerald, Yoon-Mo Yang (Hanyang University), and Katrin Karbstein @kkarbstein.bsky.social (@vanderbilt.edu): rupress.org/jcb/article/... #Biochemistry #RNAbiology

#Eosinophils promote a second wave of postnatal smooth muscle differentiation in intestinal villi. New study from Tatiana V. Petrova @petrovalab.bsky.social, Jeremiah Bernier-Latmani and colleagues @unil.bsky.social: rupress.org/jcb/article/... #Development

🔬 From our May issue: Immunofluorescence of human induced pluripotent #StemCells (hiPSCs)-derived cardiac myocytes acquired with a confocal microscope (Leica SP8). Cyan, lamin A/C; gray, DAPI (DNA); FIRE, troponin. From Kaitlyn M. Shen et al. @pennepigenetics.bsky.social: doi.org/10.1083/jcb....

Spotlight: new work from Zhao et al. (rupress.org/jcb/article/...) establishes a direct link between PI(3)P and #actin in mitochondrial dynamics. Sneha Hegde and Marc Germain @uqtr.bsky.social discuss the findings: rupress.org/jcb/article/... #Mitochondria #Lipids

Williams, @kamenzlab.bsky.social, @silkehauflab.bsky.social et al. show that synchronous chromosome segregation does not require separase-mediated positive feedback and that slight asynchrony may be unavoidable due to stochastic effects rupress.org/jcb/article/... #CellDivision #Mitosis

Mitochondrially-tethered Mmm1 can function as sole #lipid transporter at ER-#mitochondria contacts. New study from Christian Covill-Cooke, Takashi Hirashima, Shin Kawano, Toshiya Endo (Kyoto Sangyo University), Benoît Kornmann @ox.ac.uk and colleagues: rupress.org/jcb/article/... #ER_literature

Disruption of #macrophage cell volume drives inflammatory responses and type I #interferon signalling. New study from James R Cook, Jack P Green and colleagues @manchester.ac.uk: rupress.org/jcb/article/... #CellSignaling #Immunology #Inflammation

NASP functions in the cytoplasm to prevent #histone H3 aggregation during early #embryogenesis. New study from Mohit Das, Eli Coronado-Chavez, Jared T. Nordman and colleagues @vanderbilt.edu: rupress.org/jcb/article/... #Development #Chromatin #epigenetics #Biochemistry #ProteinHomeostasis

Bitesize bundles F-actin and influences #actin remodeling in syncytial #Drosophila embryo development. New study from Anna R. Yeh, Adam C. Martin @mit.edu and colleagues: rupress.org/jcb/article/... #Development #Cytoskeleton

“This recognition validates years of meticulous imaging and cell biology work uncovering a novel organelle critical for meiotic fidelity,” says Julien Dumont of being included in The Year in Cell Biology: 2025 collection. Learn more about publishing in JCB. hubs.la/Q048tT1P0