Got to play around with Holotomographic imaging of muscle cells yesterday via the @uclastemcell.bsky.social imaging core and the results are pretty spectacular 🤩 You can see everything from mitochrondria to lamellipodia to macropinocytosis…with no labels!

It really is concerning how few people know that this is the case.

TL;DR: We've identified more than 100 cases of apparent manipulation in Thermo Fisher Scientific's antibody verification data. @sholtodavid.bsky.social @johanduchene.bsky.social reeserichardson.blog/2026/05/28/h...

We invented “triplet tumbling microscopy” (TTM) to track protein-protein interactions in living cells! Tag your protein with GFP and see it interact with *untagged* binding partners www.biorxiv.org/content/10.6...

📣 The difference between replicable and not replicable is not itself scientifically replicable. 📣 New work with Erkan Buzbas, showing that verdicts such as "X% of results replicated" are based on an inferential machinery that doesn't work. arxiv.org/abs/2604.26268

Trainees jump on this! Juan is an awesome human and he’s gonna do some amazing science ✨ Can’t wait to see what he gets up to!

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

For me, the most maddening thing is when AI boosters, or detractors, all seem to agree that AlphaFold is an unmitigated good and useful tool showing the power of AI. I disagree. It's hit and miss. And the misses are REALLY expensive. Especially when the conversation suggests it is ALL HIT.

I don't think people fully appreciate how apocalyptic things are for US science. I haven't had any new funding since 2024, but I'm still ok since typical grants are for three years. This means next year I will be completely out of funding and will have to fire everyone in the lab. It's not great.

A high-affinity split-HaloTag for live-cell protein labeling. And much more. www.nature.com/articles/s41...

So excited to be drowning in new "findings". Coming soon to a journal near you! 🫠 github.com/aiming-lab/A...

Federal funding for US biomedical research is moribund. Since October 1 2025, NIH is -80% in new grants and -70% in values (total dollars). Labs are closing down and researchers are leaving science. To what end?

Do folks have a preferred protocol for bleaching fluorescent proteins prior to doing Immunofluorescence? I tried a 3% H2O2 + 12mM HCl solution but I had severe cell detachment problems (despite everything being fixed).

@science.org Evolution of error correction through a need for speed | Science #evolution 🧬🔬 www.science.org/doi/10.1126/...

Absolutely wild timeline we live in TLDR: An agentic AI bot writes a takedown blog post of a matplotlib maintainer after he rejects its pull request. @arstechnica.com screws up and use AI to write a piece about the incident which hallucinates quotes. 🤦 theshamblog.com/an-ai-agent-...

Interesting study from *Anthropic* that found that AI use didn’t significantly speed up development on a novel task (w/ new Python async lib) but it did hurt understanding. Unsurprisingly, AI seems best at regurgitating common patterns and isn’t that helpful for novel ones. arxiv.org/pdf/2601.20245

Why is it that small animals like insects have a landspace body plan when viewed head-on, but larger animals like elephants are taller than wide? With @m-v.bsky.social, we find out in our new preprint titled 'Size and shape of terrestrial animals' - arxiv.org/html/2602.00...

Really excited to share our new paper in @nature.com! We uncovered how a physical instability of the cytoplasm coupled with the cell cycle drives cytoplasmic partitioning in early embryos #zebrafish #drosophila. Read more in this🧵 www.nature.com/articles/s41... 🤩 @poldresden.bsky.social @mpi-cbg.de

For all the talk about accelerating science, tools like this will likely slow things down because we have wade through mountains of AI garbage. The consequences need to grow for faking results.

Peer review science relies, more than we care to admit, on what @zey.bsky.social calls "load bearing frictions" that make it difficult to fake good science, and on trust that most ppl also value science itself & won't cheat just for personal gain. This creates the wrong environment for that to hold.

Blog post: Chance favors the (theoretically) prepared mind Data is big, machines are learning, so what good is theory anyway? Isn't most discovery driven by serendipity anyway, with theory mostly a "post-mortem"? I argue that this view discounts the value of theory. open.substack.com/pub/arjunraj...

Good review on what’s going on at NIH. “Polling consistently shows strong majorities favor federal investment in health research. The dismantling we’re witnessing is instead the result of a political moment in which a minority viewpoint holds disproportionate power.”

New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇 www.biorxiv.org/content/10.1...

While 𝘝. 𝘷𝘦𝘳𝘮𝘪𝘧𝘰𝘳𝘮𝘪𝘴 max temp is around 45°C, we found 𝘐. 𝘤𝘢𝘴𝘤𝘢𝘥𝘦𝘯𝘴𝘪𝘴 grows up to 63°C with optimal growth at 55-57°C🥵. We showed cellular replication via Ultrastructure Expansion Microscopy at 63°C. The previous limit for euks is 60°C!

1/5: New preprint: Cytokines control the physical state of immune tissue. www.biorxiv.org/content/10.1... Unlike structured organs (heart, liver), immune tissue can shapeshift to form functional structures like germinal centers (GCs), tertiary lymphoid organs, granulomas, etc.

Really cool work! Congrats @zjmaggiexu.bsky.social and @cellraiser.bsky.social!

I had a great time presenting at the LLHF Annual Meeting. Lots of good feedback and lots to do!

mStayRed is right around the corner, amazing to see that an unnatural amino acid can bring the benefits of mStayGold to the red channel. Someone needs to engineer a way around the UAA and it’s game time.

Thrilled to announce the launch of my lab @cri-utsw.bsky.social at UTSW this January! We will explore how cells sense and respond to mechanical forces, focusing on membrane mechanics to reveal how tension and signaling work together to shape cell behavior.

Move over mScarlets, Crimson 🌶️ is the winner! It's the first red FP that I've found that properly trafficks to the membrane in primary myoblasts.

With some new sorbothane feet from @thorlabs.bsky.social, the Rando lab’s @cephlainc.bsky.social Squid+ is rapidly approaching its final form. We wrapped a cheap 3D printer enclosure off of Amazon with some matte black vinyl and it’s working great as a darkbox.

Hey #microscopy 🧪 folks. Have you all noticed similar dark current patterns with cameras where you have a nonlinear response to exposure time? 100ms and 1s exposures are extremely quiet but once I do a 60s exposure, certain pixels just get super bright.

Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy! Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social. #snouty

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...! Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more! Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

Super excited to share our new #preprint on #BioRxiv ✨ We reveal the structural basis of a partnership between the ER membrane complex (EMC) and the P5A-ATPase Spf1 — an insertase–dislocase duo that coordinates membrane protein biogenesis and quality control. www.biorxiv.org/content/10.1...

Hmm. I think there are separate--but perhaps a little overlapping--things going on here that are being somewhat conflated...

Terence Tao, youngest ever participant of the Math Olympiad, Field Medal Winner 2006, author of over 300 papers, and considered the greatest living mathematician, lost his NSF funding: mathstodon.xyz/@tao/1149568... 😞

Dug up an old movie of neutrophils solving a maze…it’s still so satisfying!

A recent paper reveals a surprising insight. The length of the cell cycle in a particular tissue determines the oncogenic transformation capacity of particular oncogenes, with tissues with shorter cell cycles most susceptible 3/n

The latest on the 🌊 complex from my PhD lab. Super cool experiments to unravel mechanistically how the WAVE complex templates lamellipodia in migrating cells. Congrats Muziyue!

If this all sounds like a calculated plan to reduce the size of the federally funding biomedical research enterprise, I believe that is exactly what is it. The notion that this "facilitates efficient management" of grants and the appropriation is, to use an official NIH term, horseshit. /fin

Brb cancelling all my nondisruptive science….we’re so back 🥳

Beautiful work on the development of protein kinase reporters. Congrats all! www.nature.com/articles/s41...

I was feeling a bit down this Fourth of July but was reminded: “Remember, it’s the birthday of our country and its ideals, not the government or any party.” Happy Independence Day! Cheers to working together for better times ahead 🥂

The hype is real. The Quest series are crazy sensitive🔬✨

Hey optical tech dev folks: how do you do alignment? Can you outline your alignment procedures for positioning optics and verifying their correct positioning?

Super cool combination of mRNA vaccine tech with biological circuit design to kill cancer cells. The future is here folks. Can we move past small molecules?

From over 300k to less than 100k. A picture of smaller, reduced science.