The Cre–LoxP system is widely used to study gene function in mice. However, Cre itself can be toxic to cells. Proper controls should always be included to distinguish gene effects from Cre toxicity. www.nature.com/articles/s44...

With hundreds of HFpEF animal studies, it’s time to ask: how good are the models? Read the comment from David A. Kass www.nature.com/articles/s44... Conclusion: current HFpEF models are suboptimal & we must refine them to better capture disease essentials and drive truly translational therapies

Scientists must learn to join larger pro-democracy and people's movements, coalitions. Our science training may not have prepared us for such work, but we can no longer sit in the safety of our labs and expect the world to get better My book review @thelancet.com www.thelancet.com/journals/lan...

Resources such as Human Protein Atlas rely on antibodies to map protein expression or intracellular localisation, but bad or unvalidated antibodies can misinform and derail research projects by giving false or unreproducible results. How can we make antibodies better and more reliable? 👇

Bad antibodies are a major roadblock in biomedical research, harming reproducibility and wasting resources. The International Antibody Validation Meeting was created to fix it. Watch the talks from the 5th Antibody Validation Meeting (2025) tinyurl.com/AntibodyVali... #Integrity #reproducibility

🗨️ Just published in Nature Biotechnology: Our CellWhisperer AI enables chat-based analysis of single-cell sequencing data. You can talk to your cells & figure out the biology without writing any computer code. Paper here: www.nature.com/articles/s41.... Annotated walkthrough in a thread below (1/11)

CD44 is involved in cell adhesion, migration & signaling. It is key in inflammation and immune diseases & cancer. Good news: most commercial CD44 antibodies are highly specific and work well for: • Western blot • Immunofluorescence • Flow cytometry Full report: zenodo.org/records/1562...

S1PR1 controls lymphocyte egress & vascular integrity and is a target for multiple sclerosis therapies. Studying S1PR1 expression using antibodies is key. Among 9 commercial antibodies tested, only ONE is highly specific for detecting S1PR1. Full report here: f1000research.com/articles/13-...

What a week! So pleased to now also see the peer reviewed version in Science Signaling of @megtriesscience.bsky.social PhD work joint with the Saunders lab, see her great thread for explanation below www.science.org/doi/10.1126/...

APOE plays a key role in cardiovascular and Alzheimer’s disease. Reliable tools are needed to study its expression and function. Among 14 antibodies tested, 4 showed excellent performance. Pick up the right one. Report: f1000research.com/articles/12-... Data summary: onlygoodantibodies.co.uk/28/

The antibodies don’t work! The race to rid labs of molecules that ruin experiments Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this. www.nature.com/articles/d41...

Many articles reported using an antibody ... that didn’t even bind the key protein in Laflamme’s testing. Those articles had been cited over 3,000 times. A key question is: ◼️ 𝐂𝐚𝐧 𝐩𝐫𝐨𝐭𝐞𝐢𝐧 𝐛𝐢𝐧𝐝𝐞𝐫𝐬 𝐚𝐜𝐡𝐢𝐞𝐯𝐞 𝐡𝐢𝐠𝐡 𝐬𝐩𝐞𝐜𝐢𝐟𝐢𝐜𝐢𝐭𝐲 𝐟𝐨𝐫 𝐞𝐯𝐞𝐫𝐲 𝐞𝐩𝐢𝐭𝐨𝐩𝐞 & 𝐩𝐫𝐨𝐭𝐞𝐨𝐟𝐨𝐫𝐦? Some investors assume an answer.

ALS is a neurodegenerative disease C9ORF72 mutation is common Understanding its biology is key Laflamme 2019 (10.7554/eLife.48363) showed: 👉Most antibodies were non-specific 👉Specific ones never used Since then: ❌ Sc-138763: discontinued ✅ Specific ones (GTX634482,GTX632041, ab221137): adopted

'All three awards (...) honored achievements rooted in fundamental research from decades ago. Some experts interpret the selections by the Royal Swedish Academy of Sciences as representing the importance of slow, basic science, work pursued out of a desire to better understand the world.'

PARKIN labels damaged mitochondria for recycling No PARKIN → no cleanup → neurodegeneration Mutations in PARK2 are common in Parkinson’s disease Accurate detection of PARKIN is crucial Problem: The most used antibodies are not selective In contrast, some very specific antibodies are not used

⚠️ Half of commercial antibodies miss their target! So how do you pick the right one for your experiment? Here’s the solution 👉 Antibody characterization data (all tested in KO cells) are now available at: 🔗 onlygoodantibodies.co.uk Tested by @ycharos.bsky.social & @oga-community.bsky.social

The myth of scientific objectivity hides the truth that every experiment is influenced by cultural values, assumptions and beliefs – and that actually hurts the search for truth Analysis: buff.ly/KcLU0bE 🧪 🩺

We've also switched from mostly transgenic mouse strains to AAV/retrovirus "transgenes". It cut mouse costs by >50%, and makes our work faster and more responsive to reviewer requests. AAVs can even be used to produce depleting antibodies. www.sciencedirect.com/science/arti...

You can also save money by cutting-costs and preventing waste. We do a low of flow cytometry, and retitrated antibodies for overnight staining, reducing costs ~100-fold while improving signal-to-noise and reproducibility currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Abstract submission and travel grant application for the 9th World Conference on Research Integrity are open. Please consider to submit an abstract and to reshare this message. lnkd.in/eJ9hmaRs

Today, our article "The entities enabling scientific fraud at scale are large, resilient, and growing rapidly" is finally published in PNAS. I hope that it proves to be a wake-up-call for the whole scientific community. reeserichardson.blog/2025/08/04/a...

Stimulating immune cells in vitro is a common experimental lab model. We profiled 150K blood immune cells treated with 11 different stimuli to compare the effects. The data are freely available for researchers. See the preprint for our findings and to access the data 🧪 doi.org/10.1101/2025...

A comparison of mouse vs human tumor microenvironments shows a major difference. Mouse TMEs have more macrophages, while human TMEs have more T cells. Chemokine expression patterns are also different. Learn more: www.biorxiv.org/content/10.1...

#FluorescenceFriday & Erythroblast Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue). Captured via Correlative Light & Electron Microscopy by @jemimaburden.bsky.social Learn more: scim.ag/4kTxFZz @antalpedro.bsky.social @jffoley.bsky.social @science.org

In our science.org/doi/10.1126/... we describe the hitherto overlooked erythroblast expression of CXCR4, which do not migrate in response to CXCR4 ligation by CXCL12. Instead, in these cells CXCR4 localises to the nucleus and mediates alternative cell responses in maturation and triggers enucleation

Not all chemokine signaling is about cell migration! In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation. www.science.org/doi/10.1126/...

Not all chemokine signaling is about cell migration! In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation. 🔗 science.org/doi/10.1126/sc…#chemokinen#CXCR4R4

Yesterday.. our #CLEM with Julia Gutjahr and Antal Rot was featured in the main Science webpage....☺️ Nice to see #correlativemicroscopy and #volumeEM up front and centre. www.science.org/doi/10.1126/...

CXCL12-stimulated intracellular and nuclear CXCR4 signaling mediates erythroblast enucleation to generate red blood cells www.science.org/doi/10.1126/...

An alternative to tSNE & UMAP for more accurate data visualization: Tree representations for distortion-free visualization and exploratory analysis of single-cell omics data. The trees are constructed to accurately represent true distances between the objects in the high-dimensional space.

#AI & national health databases offer opportunities, but can be exploited by unethical agents like #PaperMills. This study reveals an explosion of formulaic research articles, with inappropriate study designs & false discoveries, using data from US #NHANES resource @plosbiology.org 🧪 plos.io/3RWgNog

Non-specific #antibodies still plague #reproducibility in biomedical research. @YCharOS1 tackles this by testing commercial antibodies in knockout cell lines—and sharing the data openly. Learn more in their online seminar: May 29, 2025 | 4pm–5pm CEST tinyurl.com/YCharOs-Semi...

Non-specific #antibodies still plague #reproducibility in biomedical research. @YCharOS1 tackles this by testing commercial antibodies in knockout cell lines—and sharing the data openly. Learn more in their online seminar: May 29, 2025 | 4pm–5pm CEST tinyurl.com/YCharOs-Semi...

Uff

Very satisfying to see that the majority of you spotted the fake TEM image! Many noted it would've been trickier without a real image next to it to compare - unless you're a TEM specialist. With AI advancing fast, spotting fake images may soon become harder...

I voted for the left image, but this is a tough choice - genAI is getting just too good.

In partnership with RestoRes and Embrace, LORIER’s monthly webinars address key issues for reproducible health research, including data sharing, reproducibility, and meta-research. This series, starting in June 2025, features international experts. restores.univ-rennes.fr/highlights/l...

AI-generated image crisis in science. Among these 2 TEM images, only one is true! Can you tell which one was generated by AI? Left or right? @elisabethbik.bsky.social doi.org/10.1002/ajh....

5th Antibody Validation Meeting. September, 9-11, 2025. University of Bath, UK. A meeting with scientists, industry, and suppliers to discuss best practices and improve research antibody validation. antibodyvalidation.co.uk

Antibodies don't work. NLRP3 is by far the most studied inflammasome — yet <30% of commercial antibodies were specific. Only 4 of 14 tested antibodies detected NLRP3 specifically. Antibodies must be validated before use. Full report by @ycharos.bsky.social : zenodo.org/records/1162...

So excited to share our first neuro focused paper from the lab, we show that CXCL4 (PF4) is transported into the brain by proteoglycans within the endothelial glycocalyx and that GAG binding also mediates the pro neurogenesis effects of CXCL4. www.biorxiv.org/content/10.1...

Paolo Macchiarini: la mort au bout du scalpel. www.radiofrance.fr/franceinter/...

Tissue-resident memory T cells have high levels of mRNA of proinflammatory cytokines but produce the proteins only upon stimulation. 🧪⑂ The integrated stress response inhibits mRNA translation in these cells, having these cells poised for rapid responses in 🐭 and 🧓. 1/2

Introverts find solutions. 👍 #Scicomm #Neuroscience 🧪 www.mpg.de/24460941/mic...

Gerry’s lab developed iCCR mice, these mice lack the chemokine receptors CCR 1,2,3 and 5. Now they found that after infection with influenza virus, the number of CD4 T cells in the lung was strongly reduced in iCCR mice. #chemokine

Some nice new reserach from @mariekedoesscience.bsky.social and @crgglasgow.bsky.social studying the inflammatory CCR locus and immune responses to influenza. www.biorxiv.org/content/10.1...

I have a study idea that I am not competent to organise or do but I think would be very valuable to the community (unless it is already done - in that case please link!). I want a consortia of labs to separately analyse a large cancer omics dataset and present their results (1/3)

Do you think you would have found the hidden gorilla in the data? Having a hypothesis can be a disadvantage. Seeking (with a hypothesis) means having a goal. While exploring (with no hypothesis) means being free and potentially make a finding. genomebiology.biomedcentral.com/articles/10....

Could adjusting a simple blood test reference range save patients unnecessary stress? Recent research highlights Duffy null-associated neutrophil count (DANC) and the risk it imposes on certain ancestral backgrounds. Enroll in the ASH course: bit.ly/duffynull #HemeSky #Hematology #MedSky