#Medsky🧪 #Publichealth @nature.com Some disease-predictors use dodgy data . Dozens of AI models designed to predict a person’s risk of #stroke or #diabetes are trained on dubious datasets. Researchers found that many such models used one of two open-access health datasets

“All models are wrong, but some are useful” - George E.P. Box Are we misled by myocardial infarction (MI) models? Open-chest MI triggers systemic inflammation that can mask MI-specific immune responses Minimally invasive MI tells a different story Read our new preprint🧪 doi.org/10.64898/202...

🧪 #BadJournalism Headline: Genetics don’t explain variability in weight-loss jab effects “The authors’ model suggests… genetics adding only a modest incremental contribution.” Boring non-genetic factors such as sex, drug type, dose and duration most important www.theguardian.com/science/2026...

If you’re using 10x Genomics (Flex v1) for scRNA-seq, this study is a must-read. 🧪 It uncovers a key artefact: • Probe barcodes themselves introduce bias • Some barcodes systematically alter gene expression signals • Apparent DEGs may be technical, not biological www.biorxiv.org/content/10.6...

major issues with antibodies used in #neuroscience research www.thetransmitter.org/open-neurosc... #reproducibility

🔬 Are you using the right antibody for your experiments? Only Good Antibodies helps researchers identify specific antibodies 🧪 1496 antibodies against 147 proteins tested so far. Check now oga-website.onrender.com OGA is powered by YCharOS

A step forward for cardio-immunology! Our recent preprint shows: Standard MI models distort neutrophil responses through surgical inflammation. A minimally invasive model better mirrors patients and reveals immature neutrophils driving post-MI damage. Here is link www.biorxiv.org/content/10.6...

How specific are therapeutic monoclonal antibodies? In a study of 174 FDA-approved antibodies, 28% showed off-target binding. Surprisingly, even the famous pembrolizumab (anti–PD-1) binds another protein (TDGF1). www.sciencedirect.com/science/arti...

@biorxivpreprint.bsky.social A Genetic Tool for Specific Tracking of Mature Neutrophils www.biorxiv.org/content/10.6...

I'd like to see this problem analysed at the single cell multi-omics level 'Advances in research technolo- gies are crucial for the scientific enterprise but have an unintended consequence: when pow- erful techniques become widely available, they tend to be applied without a good question in mind.'

Engineers increasingly found themselves coaching colleagues who were “vibe-coding” and finishing partially complete pull requests. This oversight often surfaced informally—in Slack threads or quick desk-side consultations—adding to engineers’ workloads.' hbr.org/2026/02/ai-d...

Do we study the right biology after myocardial infarction (MI)? • Surgery in standard mouse model disrupts bone marrow & masks MI-specific immune responses • Minimally invasive model better reflects patient immune responses & reveals immature neutrophils worsen heart function tinyurl.com/MImodels

Scientific paper mill: the model flagged 10% of the research papers in cancer research as problematic 😱 www.bmj.com/content/392/...

A study shows the consequences of poor Antibody Validation - Out of 614 antibodies tested, 97 failed - These 97 non-specific antibodies (43 proteins) were used in 640 publications - 9443 mice and 5015 human samples wasted Waste of animals, human samples, time, and trust tinyurl.com/OGA-Ab

Highly recommend using CiteAb and check for genetic (KO/KD) validation too.

In all seriousness,use CiteAb and Antibodypedia, look for KO/KD validated antibodies, best - recombinant, second best - monoclonal, and if possible do your own KO/KD controls, or induction by ligand/small molecule,if not - compare hyperexpressing with low expressing cells (from depmap/protein atlas)

VA-LI-DA-TE your abs! My experience (and some spooky examples in the thread): bsky.app/profile/gree...

A new magnetic particle mouse model of stroke Importantly, no craniotomy is required, avoiding a major confounder that causes massive surgery-induced inflammation. Read the article "A minimally invasive thrombotic model to study stroke in awake mice" www.nature.com/articles/s41...

www.statnews.com/2026/02/20/c... i hope they can get the data from the authors. I was told by the editor that data weren’t available because of patient confidentiality- so many authors use that excuse , it makes data sharing policies worthless

This is weird. On @pubpeer.com, a reader noticed some seemingly irrelevant references in a Scientific Reports paper pubpeer.com/publications... The paper has now been modified, with many references removed (from 45 down to 25!) but it contains no acknowledgment of the change. It does have EoC.

Based on our publication on antibody validation, R&D Biotechne confirmed that MAB4139 and AF6695 exhibit nonspecific binding and have been discontinued. Thanks for that 👍. For assessing ACKR1 expression, the specific clones 2C3 and 6B7 should be used. #Metaresearch www.cell.com/cell-stem-ce...

The Cre–LoxP system is widely used to study gene function in mice. However, Cre itself can be toxic to cells. Proper controls should always be included to distinguish gene effects from Cre toxicity. www.nature.com/articles/s44...

With hundreds of HFpEF animal studies, it’s time to ask: how good are the models? Read the comment from David A. Kass www.nature.com/articles/s44... Conclusion: current HFpEF models are suboptimal & we must refine them to better capture disease essentials and drive truly translational therapies

Scientists must learn to join larger pro-democracy and people's movements, coalitions. Our science training may not have prepared us for such work, but we can no longer sit in the safety of our labs and expect the world to get better My book review @thelancet.com www.thelancet.com/journals/lan...

Resources such as Human Protein Atlas rely on antibodies to map protein expression or intracellular localisation, but bad or unvalidated antibodies can misinform and derail research projects by giving false or unreproducible results. How can we make antibodies better and more reliable? 👇

Bad antibodies are a major roadblock in biomedical research, harming reproducibility and wasting resources. The International Antibody Validation Meeting was created to fix it. Watch the talks from the 5th Antibody Validation Meeting (2025) tinyurl.com/AntibodyVali... #Integrity #reproducibility

🗨️ Just published in Nature Biotechnology: Our CellWhisperer AI enables chat-based analysis of single-cell sequencing data. You can talk to your cells & figure out the biology without writing any computer code. Paper here: www.nature.com/articles/s41.... Annotated walkthrough in a thread below (1/11)

CD44 is involved in cell adhesion, migration & signaling. It is key in inflammation and immune diseases & cancer. Good news: most commercial CD44 antibodies are highly specific and work well for: • Western blot • Immunofluorescence • Flow cytometry Full report: zenodo.org/records/1562...

S1PR1 controls lymphocyte egress & vascular integrity and is a target for multiple sclerosis therapies. Studying S1PR1 expression using antibodies is key. Among 9 commercial antibodies tested, only ONE is highly specific for detecting S1PR1. Full report here: f1000research.com/articles/13-...

What a week! So pleased to now also see the peer reviewed version in Science Signaling of @megtriesscience.bsky.social PhD work joint with the Saunders lab, see her great thread for explanation below www.science.org/doi/10.1126/...

APOE plays a key role in cardiovascular and Alzheimer’s disease. Reliable tools are needed to study its expression and function. Among 14 antibodies tested, 4 showed excellent performance. Pick up the right one. Report: f1000research.com/articles/12-... Data summary: onlygoodantibodies.co.uk/28/

The antibodies don’t work! The race to rid labs of molecules that ruin experiments Poorly performing antibodies have plagued biomedical sciences for decades. Several fresh initiatives hope to change this. www.nature.com/articles/d41...

Many articles reported using an antibody ... that didn’t even bind the key protein in Laflamme’s testing. Those articles had been cited over 3,000 times. A key question is: ◼️ 𝐂𝐚𝐧 𝐩𝐫𝐨𝐭𝐞𝐢𝐧 𝐛𝐢𝐧𝐝𝐞𝐫𝐬 𝐚𝐜𝐡𝐢𝐞𝐯𝐞 𝐡𝐢𝐠𝐡 𝐬𝐩𝐞𝐜𝐢𝐟𝐢𝐜𝐢𝐭𝐲 𝐟𝐨𝐫 𝐞𝐯𝐞𝐫𝐲 𝐞𝐩𝐢𝐭𝐨𝐩𝐞 & 𝐩𝐫𝐨𝐭𝐞𝐨𝐟𝐨𝐫𝐦? Some investors assume an answer.

ALS is a neurodegenerative disease C9ORF72 mutation is common Understanding its biology is key Laflamme 2019 (10.7554/eLife.48363) showed: 👉Most antibodies were non-specific 👉Specific ones never used Since then: ❌ Sc-138763: discontinued ✅ Specific ones (GTX634482,GTX632041, ab221137): adopted

'All three awards (...) honored achievements rooted in fundamental research from decades ago. Some experts interpret the selections by the Royal Swedish Academy of Sciences as representing the importance of slow, basic science, work pursued out of a desire to better understand the world.'

PARKIN labels damaged mitochondria for recycling No PARKIN → no cleanup → neurodegeneration Mutations in PARK2 are common in Parkinson’s disease Accurate detection of PARKIN is crucial Problem: The most used antibodies are not selective In contrast, some very specific antibodies are not used

⚠️ Half of commercial antibodies miss their target! So how do you pick the right one for your experiment? Here’s the solution 👉 Antibody characterization data (all tested in KO cells) are now available at: 🔗 onlygoodantibodies.co.uk Tested by @ycharos.bsky.social & @oga-community.bsky.social

The myth of scientific objectivity hides the truth that every experiment is influenced by cultural values, assumptions and beliefs – and that actually hurts the search for truth Analysis: buff.ly/KcLU0bE 🧪 🩺

We've also switched from mostly transgenic mouse strains to AAV/retrovirus "transgenes". It cut mouse costs by >50%, and makes our work faster and more responsive to reviewer requests. AAVs can even be used to produce depleting antibodies. www.sciencedirect.com/science/arti...

You can also save money by cutting-costs and preventing waste. We do a low of flow cytometry, and retitrated antibodies for overnight staining, reducing costs ~100-fold while improving signal-to-noise and reproducibility currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Abstract submission and travel grant application for the 9th World Conference on Research Integrity are open. Please consider to submit an abstract and to reshare this message. lnkd.in/eJ9hmaRs

Today, our article "The entities enabling scientific fraud at scale are large, resilient, and growing rapidly" is finally published in PNAS. I hope that it proves to be a wake-up-call for the whole scientific community. reeserichardson.blog/2025/08/04/a...

Stimulating immune cells in vitro is a common experimental lab model. We profiled 150K blood immune cells treated with 11 different stimuli to compare the effects. The data are freely available for researchers. See the preprint for our findings and to access the data 🧪 doi.org/10.1101/2025...

A comparison of mouse vs human tumor microenvironments shows a major difference. Mouse TMEs have more macrophages, while human TMEs have more T cells. Chemokine expression patterns are also different. Learn more: www.biorxiv.org/content/10.1...

#FluorescenceFriday & Erythroblast Erythroblast stimulated with CXCL12, stained for CXCR4 (green) and nuclei (blue). Captured via Correlative Light & Electron Microscopy by @jemimaburden.bsky.social Learn more: scim.ag/4kTxFZz @antalpedro.bsky.social @jffoley.bsky.social @science.org

In our science.org/doi/10.1126/... we describe the hitherto overlooked erythroblast expression of CXCR4, which do not migrate in response to CXCR4 ligation by CXCL12. Instead, in these cells CXCR4 localises to the nucleus and mediates alternative cell responses in maturation and triggers enucleation

Not all chemokine signaling is about cell migration! In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation. www.science.org/doi/10.1126/...

Not all chemokine signaling is about cell migration! In mouse erythroblasts, CXCL12-bound CXCR4 enters the nucleus to control gene transcription, trigger calcium bursts, and drive red blood cell formation—especially enucleation. 🔗 science.org/doi/10.1126/sc…#chemokinen#CXCR4R4

Yesterday.. our #CLEM with Julia Gutjahr and Antal Rot was featured in the main Science webpage....☺️ Nice to see #correlativemicroscopy and #volumeEM up front and centre. www.science.org/doi/10.1126/...