Playing around with how to represent volume in #Snouty light sheet data. Here is a dividing hiPSC with endogenously tagged histone in all three orthogonal projections. Bluer is further away. If anything, it distracts from politics.

@tanner-fadero.bsky.social told me there was a discussion about the unofficial name for #snouty V3. We have had Mr. Snouty and King Snouty, and there were people suggesting Emperor Snouty. I think it is time for a Lady or EMPRESS snouty. I have created Art to support this.

What if we could use 'any' immersion objective and get good 3D imaging? Normally we can't as we must refractive index match the immersion to the sample (previous post). -> However, I realized a few years ago that we can get good 3D imaging with any immersion using remote refocus optics!

Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.

Bluesky is now the platform of choice for the scientific community. From @jenlucpiquant.bsky.social on @arstechnica.com arstechnica.com/science/2025...

Optics folks: does anyone know a manufacturer of a dichroic beamsplitter *cube*? Essentially, a dichroic version of this (for fluorescence microscopy):

Science hive mind! Does anyone do hardware tech dev on MRI or CT? Or do you know someone who does? I'd love an introduction, if so. Let me know!

Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy! Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social. #snouty

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

Hey @thorlabs.bsky.social! Thanks for such a well-documented APT Controller Communication Protocol! It was fun coding against it. @andrewgyork.bsky.social and I did find a small bug, though. When talking to a MFF102 flipper, it only ever reports clockwise motion (never counterclockwise).

A @bob-goldstein-art.bsky.social original!

If you've ever wanted to know what the actin filaments on the scales in a developing moth wing look like in 3D, here you go! Kyle DeMarr from @mullinslab.bsky.social acquired these beautiful data on the single objective light sheet at the UCSF Center for Advanced Light Microscopy. #snouty

Finally having some time to play with ‘Snouty’ again. Thanks a million once more @tanner-fadero.bsky.social and @amsikking.bsky.social

Assume it's still done this way? www.youtube.com/watch?v=2s0O...

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

I spent some time finally deriving the relationship between lateral image magnification and axial magnification! It's an interesting equation, with dependencies on sample refractive index and the wave propogation angle w.r.t. the optical axis. Play with it yourself! www.desmos.com/calculator/q...

@kahsage.bsky.social is killing it with these teaching tools for #snoutscope training at the UCSF Center for Advanced Light Microscopy. Fantastic illustrations and a whiteboard in the room. Brilliant!

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

1/27 We have a new paper out! Turns out that snowflake yeast have been hiding a secret from us - they've evolved a (very!) crude circulatory system. Not with blood vessels or a heart, but through spontaneous fluid flows powered by their metabolism. 🧪🔬 www.science.org/doi/full/10....

Another #snoutscope imaging session at the UCSF Center for Advanced Light Microscopy! This time, with neutrophils! Courtesy of Patrick Zager in the Weiner lab. Neutrophil plasma membranes are labeled to show 3D filopodia and lamellipodia dynamics at 1 volume/second.

I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all! "This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"

Hey optical tech dev folks: how do you do alignment? Can you outline your alignment procedures for positioning optics and verifying their correct positioning?

Now out in JACS. pubs.acs.org/doi/full/10.... Grateful to thoughtful reviewers who found some errors in our model and encouraged us to make a better one! A renaissance in magnetobiology is coming...

@jennysachweh.bsky.social inspired me to make a quick and dirty model of "Two Mirror Beam Scanning" this morning. It's a nice visual demonstration of how two galvo mirrors can translate a beam with no angular motion. Try it for yourself! www.geogebra.org/calculator/s...

For using #RUSH to control and visualize membrane trafficking in Drosophila, see also shorturl.at/96Qo3 Witness the journey of cargo from the ER to the plasma membrane and out of the cell in 5 minutes:

If you train people in ImageJ, Fiji, whatever, even if it isn't your day job, please let us know, and please share this post! Thanks a ton, we really appreciate it. (2/2) forms.gle/C8dUpg8qZkbD... #bioimaging #microscopy #bioimageanalysis

It's the last #KingSnout ever! 😭 I'm looking forward to what's next in the #snouty objective lens line from ASI and Special Optics. Onwards to bigger and better things! #snoutclub @jsdaniel02.bsky.social @amsikking.bsky.social

It's the combination of speed, gentleness and high resolution in 3D that makes the Snouty light-sheet microscope so transformative! -> Here we show yeast happily dividing over ~16h. They're only ~5um in diameter but we can easily resolve the tiny vacuolar dynamics! www.nature.com/articles/s42...

By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...

🚨🧪 Are you a researcher affected by the recent cancellation of federal grants and contracts at Columbia University? I'm working on a story for @Nature.com. If you want to chat, your identity will be kept private. Signal: humbertobasilio.24 Email: [email protected] Repost please!

Do you LOVE organelles?? Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social! Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy 🧵1/N

How do tiny magnetic fields affect biochemistry? Overflowing with pride over Katherine Xiang's study on a giant magnetic field effect in a red fluorescent protein, www.biorxiv.org/content/10.1...

best microscope I ever built, who agrees?

From Lefebvre and colleagues, Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D and 3D data. www.nature.com/articles/s41...

Exciting opportunity at @CZBiohub SF! Join us as an Optical Scientist developing cutting-edge light-sheet microscopy to map vertebrate development. PhD in Optics/EE/Physics preferred. #ScienceJobs #Microscopy #BioImaging 🧪🔬💻 job-boards.greenhouse.io/chanzuckerbe...

How to build a simple alignment laser for typical optical alignment tasks: github.com/amsikking/al... -> I get asked how to align optics. I usually respond with "okay, get the alignment laser", and this is where the problems start... Good alignment needs a good alignment laser!

More tragic news about NIH and NIH science. Grant system is frozen and intramural program is being slashed. All will claw back on treatments and cures for cancer and other human diseases. This can’t be what the public wants. 🧪 www.science.org/content/arti...

Just a start! Who's missing? go.bsky.app/LAA7Sdx

I can't emphasize this resource enough! It's a great teaching tool and a great reference (because I inevitably forget and need reminding)

We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...

I recommend these optomechanics for 'O2-O3' in a single-objective light-sheet (SOLS) microscope: -> Mechanically and thermally stable. -> Relatively straightforward to align. -> 10 popular configurations. Make your own: github.com/amsikking/SO... Or buy it from @jsdaniel02.bsky.social at ASI!

NIH maximum indirect percentage just went from 60% to 15%. Research universities across the nation that rely on that overhead are about to be severely underfunded. arstechnica.com/science/2025...

Our automatic parameter-free deconvolution code is now 2–3× faster while still producing exactly the same results as before. If you want to try it out, we have a Google Notebook implementation available at beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/. Manuscript WIP.

Your Action Is Needed! Congress needs to hear from scientists. Call your representatives to oppose funding freezes and restrictions on research. Find contact info at govtrack.us and make your voice heard today! #SciencePolicy www.ascb.org/science-poli...

Good news! You can now buy the @thorlabs.bsky.social QS20X Ø20 mm galvo with λ/4 flatness and E02 coating! -> Great for scanning AND projecting volumes in a single-objective light-sheet (SOLS) microscope! #Snouty! Part: G-N-2257 Description: "1D Galvo System, Dielectric, 400-750 nm, X-Orientation"

Who else has a #Snouty objective? I currently have 25 members in #SnoutClub! But I think there's a LOT MORE out there... Anyone else care to join? -> Just reply here with a pic of your #Snouty and I'll add you! Why? It helps us promote and develop the technology! amsikking.github.io/snoutclub/