MagLOV quantum sensing update! Much improved imaging (>10% single cell ODMR contrast), detailed characterisation and simulation, and new experimental demonstrations taking us a step closer to applications. www.biorxiv.org/content/10.1... SI: www.biorxiv.org/content/10.1...

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

Very lucky to be at UCSF with such great microscopy facilities. These instruments are definitely pushing the limits of what we thought feasible in live imaging! Excited to keep exploring how these single cells organize and secrete such precise morphologies.

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all! "This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"

HIGH-RESOLUTION IN VIVO BRAIN IMAGING OF DROSOPHILA 🪰 New Preprint by Tassara et al.! www.biorxiv.org/content/10.1... #FluorescenceMonday #SnoutClub 1/6

I recommend these optomechanics for 'O2-O3' in a single-objective light-sheet (SOLS) microscope: -> Mechanically and thermally stable. -> Relatively straightforward to align. -> 10 popular configurations. Make your own: github.com/amsikking/SO... Or buy it from @jsdaniel02.bsky.social at ASI!

Who else has a #Snouty objective? I currently have 25 members in #SnoutClub! But I think there's a LOT MORE out there... Anyone else care to join? -> Just reply here with a pic of your #Snouty and I'll add you! Why? It helps us promote and develop the technology! amsikking.github.io/snoutclub/

I'll be telling stories about science tonight in San Francisco. Come through! partiful.com/e/ldwWPLR2fO... The real draw is the crew hosting me, though. The local biotech hacker/maker/entrepreneur scene is PACKED with brilliant, inspiring folks. No slides, just stories.

This is such a fun project! I love the kind of work that F.R.O.s attack.

MagLOV! MagLOV is a fluorescent protein you can "turn off" with a handheld magnet, engineered by @mariaingaramo.bsky.social. We think/hope we can use it to turn arbitrary proteins (including drugs!) on and off in opaque creatures (including humans!). bsky.app/profile/addg...

"Magnetic control of the brightness of fluorescent proteins" 🧲💡 Andrew G. York is one of very few scientists, who are not just scientists - but also real inventors! Now, fluorescent proteins that can be tuned by a magnet. 😲 andrewgyork.github.io/gfp_magnetof... #Microscopy #FluorescentProteins

If you like engineering fluorescent proteins, you'll want to check out our latest blog post! The York lab's MagLOV is a fluorescent protein that responds to magnetic fields, a promising starting point for a potential new class of tools. blog.addgene.org/mag...