A day in the life of a growing hiPSC spheroid expressing endogenously mStayGold-tagged histone 3.3 for #FluorescenceFriday and the #Snouty aficionados. 28 hours (and >50,000 exposures) later and they are still going! Home-brewed volume projection.

We invented “triplet tumbling microscopy” (TTM) to track protein-protein interactions in living cells! Tag your protein with GFP and see it interact with *untagged* binding partners www.biorxiv.org/content/10.6...

MagLOV 2 plasmid is (finally!) available on Addgene 🎉🧲🧫 Any GFP capable imaging setup + benchtop power supply + off the shelf electromagnet is all you need to get started - let us know if you need help of course. www.addgene.org/252303/ @andrewgyork.bsky.social @mariaingaramo.bsky.social 🧪

Meet MagLuc: a magnetically-controlled luminescent protein. This is a foundational tool for remote control of biological processes, also known as "magnetogenetics". Can you tell when the magnetic field turns on and off? From @mariaingaramo.bsky.social and the team at @nonfictionlabs.bsky.social

I concur with Andrew's skepticism.

I've been getting questions about this paper all week: www.cell.com/cell/abstrac... They make EXTRAORDINARY claims - fortunately, these are trivial to verify/falsify. Unfortunately, there's other concerns.

Quantum biological control, now in a multicellular organism! Super exciting to see this work from @scburd.bsky.social et al 🧪

Researchers have engineered magnetically controlled fluorescent proteins that can be remotely dimmed and brightened in cells and living animals go.nature.com/3NUSY12

‘Remote controlled’ proteins illuminate living cells www.nature.com/articles/d41... 🧪

Our research on magneto-sensitive fluorescent proteins and some of their applications has now been published! Huge thank you to the many many people involved in making this happen. 🧪 www.nature.com/articles/s41...

Our snouty v3 (AMS-AGY v3) objective arrived today! Looking forward to getting it installed on our single objective light sheet system very soon...

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody. Its binding turns on and off with a magnet. This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

We’re thrilled to feature Dr. Tanner Fadero from the Chan Zuckerberg Imaging Institute in an upcoming Nature-hosted webinar about... PHOTONS! 😎 Join the webinar to learn and take part in the live Q&A. 👉 Register here: https://ow.ly/vsyn50XkZ88 #FluorescenceMicroscopy #LightSheetMicroscopy

MagLOV quantum sensing update! Much improved imaging (>10% single cell ODMR contrast), detailed characterisation and simulation, and new experimental demonstrations taking us a step closer to applications. www.biorxiv.org/content/10.1... SI: www.biorxiv.org/content/10.1...

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

Very lucky to be at UCSF with such great microscopy facilities. These instruments are definitely pushing the limits of what we thought feasible in live imaging! Excited to keep exploring how these single cells organize and secrete such precise morphologies.

Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.

I got a text from Yovan Bodal, who just used our #snoutscope @UCSF to image fly embryos. His quote says it all! "This was the best results we've gotten on any instrument, possibly for the least amount of work on my end. Here's a GIF of individual mRNAs coming off the gene as they get transcribed:"

HIGH-RESOLUTION IN VIVO BRAIN IMAGING OF DROSOPHILA 🪰 New Preprint by Tassara et al.! www.biorxiv.org/content/10.1... #FluorescenceMonday #SnoutClub 1/6

I recommend these optomechanics for 'O2-O3' in a single-objective light-sheet (SOLS) microscope: -> Mechanically and thermally stable. -> Relatively straightforward to align. -> 10 popular configurations. Make your own: github.com/amsikking/SO... Or buy it from @jsdaniel02.bsky.social at ASI!

Who else has a #Snouty objective? I currently have 25 members in #SnoutClub! But I think there's a LOT MORE out there... Anyone else care to join? -> Just reply here with a pic of your #Snouty and I'll add you! Why? It helps us promote and develop the technology! amsikking.github.io/snoutclub/

I'll be telling stories about science tonight in San Francisco. Come through! partiful.com/e/ldwWPLR2fO... The real draw is the crew hosting me, though. The local biotech hacker/maker/entrepreneur scene is PACKED with brilliant, inspiring folks. No slides, just stories.

This is such a fun project! I love the kind of work that F.R.O.s attack.

MagLOV! MagLOV is a fluorescent protein you can "turn off" with a handheld magnet, engineered by @mariaingaramo.bsky.social. We think/hope we can use it to turn arbitrary proteins (including drugs!) on and off in opaque creatures (including humans!). bsky.app/profile/addg...

"Magnetic control of the brightness of fluorescent proteins" 🧲💡 Andrew G. York is one of very few scientists, who are not just scientists - but also real inventors! Now, fluorescent proteins that can be tuned by a magnet. 😲 andrewgyork.github.io/gfp_magnetof... #Microscopy #FluorescentProteins

If you like engineering fluorescent proteins, you'll want to check out our latest blog post! The York lab's MagLOV is a fluorescent protein that responds to magnetic fields, a promising starting point for a potential new class of tools. blog.addgene.org/mag...