pGP-AAV-syn-iGABASnFR2(no bind)-WPRE Depositor: GENIE Project AAV-mediated expression of improved GABA sensor control (no change in fluorescence) In-stock AAV preps available in AAV1 and AAV5; other serotypes available on demand. https://twp.ai/4it0eQ

We’re looking forward to the Society for Neuroscience (@sfn.org) conference in San Diego next week! Our team will be catching talks on everything from neuronal cell types to pathways of neurodevelopment and degeneration — and we’d love to connect while we’re there. Here’s where to find us:

Come discuss antibodies with @ipiproteins.bsky.social at #SfN25! Join us Monday, Nov 17 (11–3) in booth # 3625 for IPI office hours. Let’s chat about new tools for axon guidance & the synaptic cleft — and what antibodies you want to see next.

Thanks for sharing your insight with the community!

Glypicans, complete! We now have recombinant antibodies targeting GPC1–6, giving researchers full coverage of this key family. Thanks to our partners at IPI! Extensive characterization available: https://twp.ai/4ispyb

New from the Hirokazu Hirai lab (Cell Rep Methods, 2025): an AAV vector engineered for specific and efficient gene expression in microglia. https://twp.ai/4ispvR

Are you presenting research at #SfN25 that uses materials available through Addgene? Join our Addgene Ambassadors program! Learn more: https://twp.ai/4ispwV

Going to #SfN25? Stop by booth #3625 to meet the Addgene team and hear what’s new. We’re so excited to see everyone in person again! https://twp.ai/4irXYw

Need a big toolkit for your next cloning project, a whole palette of biosensors or fluorescent proteins, or expression constructs spanning your favorite pathway? Addgene kits may be just what you need!

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is our combo fibrillarin and nucleophosmin! 🧫 Our cell lines & plasmids are available for just the cost of shipping to accelerate scientific discovery. 📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social#FluorescenceFriday

Experimental reporter tool: Nucleic Acid Therapeutic Unlabeled Reporter Assay (NATURA) Available on @addgene.bsky.social www.addgene.org/226557/

Our President & Executive Director Chonnettia Jones sat down with Pulse 2.0 to discuss all things Addgene–including the organization’s evolution, our commitment to scientific sharing & even some standout moments in our history that have fostered scientific breakthroughs.

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is FUS (RNA binding protein)! 🧫🧬 Our stem cell lines and plasmids are fluorescently-tagged to illuminate cellular structures and available for just the cost of shipping. 📦 Distributed by @coriellinstitute.bsky.social and @addgene.bsky.social#FluorescenceFriday

Next Friday - Oct 31 - our President & Executive Director Chonnettia Jones will speak at the Powering Progress Through Partnership Business Conference. Don’t miss the session, “Breaking Barriers: LGBTQ Leadership in Corporate America.” 🔗 https://twp.ai/4irJfd

Developers, it’s live 👇 The Addgene Developers Portal gives you API access to plasmid + sequence data. Query, integrate, and build with Addgene’s plasmid data. Docs + access:

Not clickbait 🖱️ build and explore proteins and other biological components faster than ever! Professor Mark Howarth defines Click Biology — standards for building biological tools that’s analogous to Click Chemistry — in our latest blog post! @HowarthSci

pME-PLIN2-tdTOMATO Depositor: Richard White Purpose: PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning https://twp.ai/4ir7jF

Do you wish you could build a protein as easily as you can build a LEGO set? With tools like SpyTags, SNAP-tags, and inteins, you can! Learn about how these tools fall under “Click Biology,” a paradigm for exploring biological components, in our new blog post! @HowarthSci

pAAV-Ef1a-fDIO-ChrimsonR-tdTomato Depositor: Patricia Jensen Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination In-stock AAV preps available in AAV1 and AAV9; other serotypes available on demand. https://twp.ai/4ir8j7

Looking for tools to control gene expression or build new models? Explore Addgene’s collection of Cre-lox and other site-specific recombinases (Flp, Dre, and more) to find the right system for your research. https://twp.ai/4ir8li

Thanks for sharing your tools! 🧬🧑‍🔬 Find the plasmids here: www.addgene.org/browse/artic...

Need to pump up your protein purification yields, visualize where your protein is going, or identify which partners your protein likes to hang out with? Check out our curated guide to Bacterial Expression Systems:

The growing Promega Collection supports applications from protein-protein interactions to targeted protein degradation and bioluminescent assays. Available to both academic & industry researchers, as stabs or purified DNA. No MTA required. https://twp.ai/4is86M @promegacorporation.bsky.social

Barcode and image with PRISM! 🧠🌈🔬 Use AAVs to label neurons with thousands of unique combinations of protein epitope-tag barcodes for neuronal mapping and connectomics. Plasmids developed by @e11bio.bsky.social now online!

SUMO3_pcDNA6.2/EmGFP-Bsd Depositor: Roger Reeves Purpose: Mammalian expression vector encoding SUMO3 and EmGFP-Bsd https://twp.ai/4is88u

Access Addgene’s plasmid data like never before. The new Developers Portal lets approved users download or query our catalog programmatically - with bulk JSON datasets and API access.

We love sharing tools developed by @hhmijanelia.bsky.social GENIE Project! 🧠🔬🧬 Find their plasmids with biosensors for calcium, GABA, glutamate, and more: www.addgene.org/GENIE_Project/

What makes luciferase such a versatile reporter? 🔬 This primer covers how luciferase works, different variants available, and ways to use it to track gene expression and cellular pathways. 🔗 Read the blog:

pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt Depositor: Kaspar Podgorski Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI In-stock AAV preps available in AAV1; other serotypes available on demand. https://twp.ai/4is85w

On-again, off-again with your favorite gene? Maybe some new plasmids are just what your experiment needs! Check out our recently updated Tetracycline Inducible Expression collection page! 🧬: https://twp.ai/4is3BR

From reporter assays to live-cell imaging, NanoLuc® luciferase has transformed how scientists measure and monitor biology. Explore the technologies it powers — and why it’s a staple in molecular biology research. 🔗 Read the blog: https://twp.ai/4is3BI @promegacorporation.bsky.social

New AAV plasmids from @nadavahituv.bsky.social lab - used for CRISPRa in adipocytes https://twp.ai/4is3DE

pBiT3.1-N_DDB1:1-1140 Depositor: Cheryl Arrowsmith Purpose: Mammalian expression of full-length human DDB1. N-terminal HiBiT tag. https://twp.ai/4is1ck

Find these amino acid reporters here! 🧬🧪https://www.addgene.org/browse/article/28244242/

Chonnettia Jones, Addgene’s President & Executive Director, is one of Endpoints News’ 2025 Top Women in Biopharma R&D! She is driving Addgene’s growth to enable more discovery and innovation, powered by the sharing of scientific resources. 🔗

Doing Western Blots in lab classes today. Send me your "best" (aka worst) WBs for me to show my students how not easy they are 😆

Choosing the right dye or ligand can make or break an experiment. 💡 This guide from @promegacorporation.bsky.social breaks down the strengths and tradeoffs — helping you make the best match for your assay. 🔗 Read the blog:

pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE Depositor: GENIE Project Syn-driven, Cre-dependent expression of the improved GABA sensor control iGABASnFR2(no bind) (no change in fluorescence). In-stock AAV preps available in AAV1 and AAV5; other serotypes available on demand. https://twp.ai/4irnYg

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is SMC (structural maintenance of chromosomes) protein 1A! 🧬🛠️ Our cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery. 📦 Dist. by @coriellinstitute.bsky.social & @addgene.bsky.social

New CRISPR libraries from the @johndoench.bsky.social and Root labs — Jacquere (human) and Julianna (mouse). A perfect pairing for your next screen. 🍷🧀 https://twp.ai/9PX2kT https://twp.ai/4irnYP

Thanks for sharing the plasmids at Addgene! www.addgene.org/browse/artic...

HiBiT takes split-luciferase assays to a whole new, highly-sensitive level! 💡 Read about the many applications enabled by HiBiT in this guest blog from our friends at @promegacorporation.bsky.social

New AAV tools from @e11bio.bsky.social! PRISM uses protein-tag barcodes for high-throughput reconstruction of neurons and other cell-labeling applications. https://twp.ai/4iruEB

We've just added 250+ more plasmids to the Promega Collection • Human ORFs with tags like NanoLuc®, HaloTag®, SmBiT, and LgBiT • NanoBiT® and NanoBRET® plasmids for live-cell assays • Reporter vectors for gene expression and pathway analysis https://twp.ai/4iriLO

AiP13987: pAAV-AiE0452h_3xC2-minBG-jRGECO1a-WPRE3-BGHpA Depositor: AIBS, Jonathan Ting Expression of jRGECO1a in striatal indirect pathway medium spiny neurons. In-stock AAV preps available in PHPeB; other serotypes available on demand. https://twp.ai/4iriLD

Newly released plasmids from @vikpc.bsky.social, Phil Sharp, and Robert Langer: next-generation prime editors (vPE) with strikingly low error rates. https://twp.ai/4iriKs

Anti-FTH1 [GP5] Depositor: Melina Fan Anti-Ferritin heavy chain 1 (Human) recombinant mouse monoclonal antibody https://twp.ai/4iqKGE

Baby KJ's metabolic disorder was treated through an intense six-month effort to create a personalized CRISPR base editor — made possible by the preceding nine years of base editing research. Learn about the advances that went into this year's major milestone.

Protein labeling made simple. Self-labeling tags like HaloTag® let you visualize proteins in real time with the dye or ligand of your choice. Find out how these systems expand your experimental toolkit. 🔗 Read the blog: