Protein labeling made simple. Self-labeling tags like HaloTag® let you visualize proteins in real time with the dye or ligand of your choice. Find out how these systems expand your experimental toolkit. 🔗 Read the blog:

LjUBQ:MtN26_:T35s Depositor: Wolf Scheible Purpose: Overexpression of Peptide coding gene (Binary vector) https://twp.ai/4iqKDp

Visit our IPI antibody collection to find IPI's epitope tag antibodies and many more! www.addgene.org/antibodies/p... 🔬 Explore real application data from actual experiments with every antibody.

Cool science alert! A plasmid encoding a photodegradable hydrogel-forming protein called PhoCoil. Find it here: https://twp.ai/4iqKMK @coledeforest.bsky.social

3 color confocal image of a #Drosophila 🪰embryo stained for chitin (🟦STAR RED), tubulin (🟥STAR ORANGE), and DNA (🟩PicoGreen) #fluorescencefriday

A plasmid is available from Addgene (www.addgene.org/104586/) to create the protein which can then be conjugated with a SNAP-ligand of choice (see link.abberior.rocks/xgtw9).

Tags, reporters, luciferase — oh my! To celebrate the expansion of the Promega Plasmid Collection, we’re spotlighting some of our most popular blogs on the technologies researchers rely on every day — tools you can access through Addgene.

AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) Depositor: AIBS, Jonathan Ting SYFP2 expression in forebrain GABAergic interneurons. In-stock AAV preps available in PHPeB; other serotypes available on demand. https://twp.ai/4iqKDj

Check out the plasmids from this study! 👉 www.addgene.org/browse/artic...

Find the plasmids containing dMCP and dPCP here! www.addgene.org/browse/artic... Thank you @johntngo.bsky.social for sharing 🧬

Discover plasmids from the Allen Institute! www.addgene.org/depositor-co...

The expanded Promega Collection is here! 250+ new plasmids for cancer research, protein interactions, targeted degradation & more. ✔ Industry + academic access ✔ No MTA required ✔ Aliquots or stabs to fit your workflow Check it out: https://twp.ai/4iqKEV

Science is a team effort, and the Allen Institute has put in a lot of effort! They screened thousands of AAV enhancers to find the best and brightest, culminating in the AAV Enhancer Collection at Addgene. Learn about more of the details in our blog post!

New at Addgene! Three new yeast toolkits are now available for the community. Details in thread ⬇️ https://twp.ai/4iqKvQ

Want to understand why PAM-relaxed Cas9 variants like SpRY are inefficient? Check out this paper and the associated plasmids!

Rapid advances in base editing led to a personalized CRISPR therapy for baby KJ. Learn how new tools and collaboration made this breakthrough possible:

L1P46 Depositor: Jennifer Nemhauser Purpose: Level 1 PhiC31 cassette with constitutive promoter: pPP2AA3:PhiC31:t35S https://twp.ai/4iqPHI

How do we measure the impact of data sharing? We don’t fully know — but our ED Chonnettia Jones and a team of #openscience experts are working on it. Their S-Index framework was named a finalist in the NIH Challenge. More:

Baby KJ's metabolic disorder was treated through an intense six-month effort to create a personalized CRISPR base editor — made possible by the preceding nine years of base editing research. Our latest blog post lays out the timeline of the advances that went into this year's major milestone.

AAV-hSyn-Soma-jGCaMP8s Depositor: Marianne Fyhn hSyn-driven expression of the soma-targeted calcium sensor GCaMP8s. In-stock AAV preps available in AAV1, AAV9, and AAVretro; other serotypes available on demand.

What is open science? Watch this explainer video from @alleninstitute.org!

New tools available! Plasmids deposited by the Mingshan Xue Lab expand the optogenetics toolkit with potent, blue-shifted channelrhodopsins. https://twp.ai/4iqCaZ

Thanks for sharing these new biosensor plasmids for phosphatidic acid! Find them all here www.addgene.org/browse/artic...

Sometimes less is more. With our new smaller size option for AAV Packaged on Request, get the convenience of our QC'd and ready-to-use AAVs in the volume that meets your needs. Explore over 4,000 eligible cargo plasmids and 7 available serotypes:

Earlier this year we released the Allen Institute for Brain Science AAV Enhancer Collection. Read about all the juicy details of how this collection was put together in our latest blog post! @alleninstitute.org

pCSm5En2DER4(#1062) Depositor: Sophie Vriz Purpose: Cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis https://twp.ai/4iq6lF

New serotypes are here! With more options in AAV Packaged on Request, you can target a wider range of cells and tissues – and unlock new experimental possibilities. Read more about tissues and tropisms in our blog:

Science is a team effort, and the @alleninstitute.org has put in a lot of effort! They screened thousands of AAV enhancers to find the best and brightest, culminating in the AAV Enhancer Collection at Addgene. Learn more about all of the details in our newest blog post!

GreenPy1 (GFP) & ApplePy1 (RFP): new pyruvate biosensors from @campbell-lab.bsky.social for multicolor imaging of metabolism. https://twp.ai/4iqyg5

Find the CrAAVe-seq plasmid pAP215 here: www.addgene.org/217635/ Exciting work, thanks for sharing!

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is nucleophosmin 1 - illuminating the nucleolus! 🧫 Our cell lines & plasmids are available for just the cost of shipping to reduce the barriers to scientific discovery. 📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social

Anti-Kv1.2 K+ channel [K14/16R] Depositor: James Trimmer Anti-Kv1.2 K+ channel (Human) recombinant mouse monoclonal antibody https://twp.ai/4iqqxL

With 7 serotypes to choose from – including the new AAV6 (in vitro standout) and AAV11 (unique in vivo performer) – Addgene's Packaged on Request service gives you more ways to match the right vector to your research. Browse over 4,000 eligible plasmids today: https://twp.ai/4iqxM0

Adenoviruses are highly infectious, which is handy for getting transgenes into cells, but not great for safety. Luckily, they have been engineered into adenoviral vectors for safe use in the lab. Learn how in our updated adenoviral guide!

Why is labeling your samples important? If you've ever shared a fridge or freezer with lab mates, you know why! If you're still lost (and you've lost your samples), watch our Quick Tip on Labeling in the Lab.

Check it out - and find the PhoCoil plasmid here! www.addgene.org/browse/artic... Thanks for sharing, @coledeforest.bsky.social 😃

Attention immunologists! The TCR Rapid Assembly for Functional Testing (TCRAFT) Libraries from the Michael Birnbaum Lab are now available. A faster, lower-cost way to generate T-cell receptor libraries for TCR–antigen screening.

pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA Depositor: Guoping Feng Expresses nuclear localized tdTomato under CAG promoter https://twp.ai/4iqqvU

Congrats to the Fawzi Lab (@nicolasluxfawzi.bsky.social)! Their 2nd plasmid hit blue flame status! Big thanks to all contributors. These plasmids help study TDP-43 & FUS genes tied to ALS, FTD & Alzheimer’s. Learn more: https://twp.ai/9PWCWR https://twp.ai/4iqxKN

Save time and get to your work faster! Select from over 4,000 eligible AAV cargo plasmids, 7 serotypes, and 4 volume options – receive QC'd and ready-to-use research-grade viral vector preps produced by our experts. Find out more: https://twp.ai/4iqxGs

Last minute plans for summer's end? Read our Hot Plasmids blog post and: 🏊Take a dip in the pool(ed library) 😎Soak up the rays (of a bright new fluorescent protein) 🧳Pack up (your AAVs) and grab your ATLAS for a road trip! 🌱Edit plant genomes with ease (okay, not everything can be a summer pun)

We've got educational resources for scientists and trainees at every level! For a start, check out our Quick Tips videos here: www.youtube.com/watch?v=8lLu...

Quick heads-up: our U.S. office will be closed tomorrow (Monday, Sept 1) for Labor Day. 🇺🇸 We’ll be back online Tuesday to help with your orders and questions. Wishing you a safe and relaxing holiday (and a smooth Monday to our friends outside the U.S.)!

Find this plasmid and more in the Allen Institute for Cell Science plasmid collection! www.addgene.org/depositor-co...

The plant expression constructs for the NADPH:NADP+ NAPstar family biosensors by @jan-oleniemeier.bsky.social are now available via @addgene.bsky.social www.addgene.org/browse/artic...

There's always space in the toolbox for a new tool... especially when that new tool is a bright, sensitive orange calcium indicator! Read about OCaMP in this guest blog post by @abhiaggarwal.bsky.social

Anti-Pan-QKI [N147/6.1R] Depositor: James Trimmer Anti-Pan-QKI (Human) recombinant mouse monoclonal antibody https://twp.ai/4iqozW

If you’ve previously checked out our Packaged on Request service, check again! We’ve expanded our offering to over 4,000 eligible plasmids, 7 serotypes, and 4 volume sizes. More targets. More flexibility. Same trusted QC. See what’s new: https://twp.ai/4iqcnQ

Addgene can package AAV vectors on request — but what are AAV vectors, and why should you use them? Whether you’re new to AAV or refining your knowledge, our newly updated AAV guide will help you answer your questions!

It's summer time and things are heating up! Bright fluorescent proteins, plant genome editors, new viral tools, and more. That's right, it's our latest Hot Plasmids post! Read about these and other hot new tools on our blog.