💚 Sharing makes our future as bright as these biosensors 💚

Anti-Olig1 [N149/25.1R] Depositor: James Trimmer Anti-Olig1 (Mouse) recombinant mouse monoclonal antibody https://twp.ai/4itsJ8

Find the antibodies here! 🔬🧑‍🔬 www.addgene.org/antibodies/p...

pGEL851 Depositor: Yong Zhang Expression of TadCBEd in rice cells https://twp.ai/4itsIh

pAAV_BiLAMP5e3_jGCaMP8m Depositor: Gordon Fishell Expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer In-stock AAV preps available in AAV1; other serotypes available on demand. https://twp.ai/4ittG2

Oh where, oh where does my protein localize? Oh where, oh where can it be?🎵 Check out our Subcellular Localization Collection page, with fluorescent protein markers for organelles and cellular structures to help you find your way 🔬

🤩 We love seeing agar stabs arrive at the lab! Thanks for the explainer and great tips @bumblingbiochemist.bsky.social

pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61) Depositor: Benjamin Blencowe Purpose: Protein expression in insect cells https://twp.ai/4isUN9

pAAV_BiPVe4_eGFP Depositor: Gordon Fishell Expressing eGFP driven by chandelier cell-targeting enhancer In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand. https://twp.ai/4isUN5

We always love new plasmids coming online! 💖 Thanks for sharing @schmittlab-ucla.bsky.social

🧠🧬 CRISPR editing in postmitotic cells like neurons is hard. But the Fellman, Conklin, @kampmann.bsky.social, @doudna-lab.bsky.social labs and colleagues @innovativegenomics.bsky.social have found strategies to make it work! Find plasmids here: https://twp.ai/4isUMv

Congratulations to @addgene.bsky.social depositors @jourdainlab.bsky.social, @mpercharde.bsky.social, and @alejofraticelli.bsky.social (and maybe some future Addgene depositors?) on this honor! www.addgene.org/Alexis_Jourd... www.addgene.org/Michelle_Per... www.addgene.org/Alejo_Rodrig...

Congrats! Thanks for sharing your tools with so many scientists around the world. Sharing speeds science!

AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA Depositor: Allen Institute for Brain Science, Jonathan Ting Nuclear SYFP2 labeling in neurons, for nuclear isolation and scRNA-seq applications In-stock AAV preps available in PHPeB; other serotypes available on demand. https://twp.ai/4isPbK

New at Addgene! Photoswitchable HaloTag, enabling robust and selective labeling of HaloTag on the surface of living cells using visible-light activation. From the @clairedeo.bsky.social lab. Walterspiel et al. Angew. Chem. Int. Ed. 2025 https://twp.ai/4isOad

Happy #FluorescenceFriday! Our #CellLineOfTheWeek is PCNA - illuminating a protein involved in DNA replication and repair! Our cell lines & plasmids are available at cost to support discovery: https://www.allencell.org/cell-catalog.html 📦 @coriellinstitute.bsky.social and @addgene.bsky.social

Thank you to the 6,700 researchers who deposit their plasmids with us — your contributions help build a more connected scientific community & unlock the full potential of research! Addgene will be closed Thurs., Nov. 27 & Fri., Nov. 28 for the Thanksgiving holiday.

pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH Depositor: Ivo Spiegel Kir2.1 expression for silencing neurons and mScarlet-KASH expression In-stock AAV preps available in AAV8; other serotypes available on demand. https://twp.ai/4itAvh

New at Addgene! MitoTRACER, a cre-lox based reporter of cell-to-cell mitochondrial transfer. Used to study transfer from nerve to cancer cells and its impact on metabolism and metastasis. By @greletlab.bsky.social 🔗 https://twp.ai/4itAyW

Happy #FlourescenceFriday! Our #CellLineOfTheWeek is SON, a cell line and plasmid that illuminates ✨ nuclear speckles ✨ a real science word for nuclear proteins involved in gene expression. 📦 @coriellinstitute.bsky.social and @addgene.bsky.social#FlourescenceFriday

Anti-Kvbeta2/KCNAB2 K+ channel [K17/70R] Depositor: James Trimmer Purpose: Anti-Kvbeta2/KCNAB2 K+ channel (Mouse) recombinant mouse monoclonal antibody https://twp.ai/4it5U8

Brie and Brunello, Calabrese and Dolcetto, Julianna and Jacquere! 🧀🍷 Are we hosting a wine tasting? Not exactly… These are all pooled plasmid libraries for genome-wide screening!

5'-3xFLAG Msl1-exon minimal CMV pCDNA5 Depositor: Benjamin Blencowe Purpose: LR vector for integration of Msl1-exon into N2a FRT rtTA3 expression cells https://twp.ai/4it5R3

Who doesn't love a library? A pooled library, that is! Grab a hot drink and your reading glasses and enjoy browsing the libraries Addgene has to offer: https://twp.ai/4it3AO

Addgene's site is functional once again! 👩‍💻 We will provide an update if we identify any other issues. addgenestatus.org

Addgene's site is currently unavailable due to the issues with Cloudfare's cybersecurity service. You can check the status of our site as Cloudfare works to resolve these issues: addgenestatus.org

pAAV_BiVIPe4_ChR2_mCherry Depositor: Gordon Fishell Expresses mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. In-stock AAV preps available in AAV1 and PHPeB; other serotypes available on demand. https://twp.ai/4it7qP

Happening soon at #SfN25! 🧠 Come chat about antibodies with @ipiproteins.bsky.social during IPI office hours — booth #3625, 11–3. Let’s talk axon guidance, the synaptic cleft, and the next antibodies you want to see from Addgene × IPI.

We’re live at #SfN25! 🧠 Be sure to stop by booth #3625 to chat with our onsite Addgenies, grab some swag, and celebrate open science with us.

Heading to #SfN25? Look for some of the labs and hot #neuroskyence tools featured in our latest blog post!

MAGIC Toolbox: MultiSite Assembly of Gateway Induced Clones ✨🧬 Thanks for sharing @wythelab.bsky.social! Find the plasmids here: www.addgene.org/browse/artic...

New recombinant monoclonal antibodies from @ipiproteins.bsky.social at Addgene targeting Neurexin and Neuroligin — key mediators of synaptic adhesion and function. Explore the collection and advance your neuroscience research: https://twp.ai/4itA72 #neuroscience #neuroskyence

We're always excited to welcome new depositors! Thanks for sharing your plasmids @diegodibi.bsky.social

🚨IPI just released new antibodies into the @addgene.bsky.social catalog targeting Neurexin 1/2/3 & Neuroligin 1. Order now! buff.ly/CpP2b8x #ProteinScience #Neuroscience #Antibodies

We’re heading to San Diego for #SfN25 and we can’t wait to meet with you! Stop by booth # 3625 to connect with our team and find out what’s new. https://twp.ai/4it0f4#SfN25

Addgene was featured in MassBio’s latest member spotlight! Based in Watertown, Addgene is proud to be a MassBio member & partner with so many MA-based organizations & researchers who are reinforcing the state as an integral part of the life sciences innovation ecosystem.

Need a better way to purify your favorite plant proteins? 🎣 Check out the Catch & Release toolbox! Find plasmids here: www.addgene.org/browse/artic... Thanks @kunzlab.bsky.social for sharing!

We’re looking forward to #SfN25! 🧠 Tell us about your research using materials available through Addgene, so we can celebrate your science! Learn more: https://twp.ai/4it0di#SfN25!

Cool work from our neighbors at @cultivarium.bsky.social using components from the POSSUM Toolkit! What will they (or you!) find next? Check out the toolkit here: www.addgene.org/kits/cultiva...

pCMV_N7-BE4-puro Depositor: Gang Wang Purpose: Express N7-BE4 base editor in mammalian cells https://twp.ai/4it0fC

Check it out!💡🦋 And find the plasmid for Minos transgenesis with mBaoJin here: www.addgene.org/browse/artic... Thanks for sharing @evolvwing.bsky.social!

Thanks for sharing @piatkevich.bsky.social! 💡 These red calcium indicators and more are featured in our Fluorescent Protein Biosensors Collection: www.addgene.org/fluorescent-...

Fresh batch of Hot Plasmids here! Find new tools for: 🧠 Neural cell-surface proteomics 🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social 🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social ✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social & more!

pGP-AAV-syn-iGABASnFR2(no bind)-WPRE Depositor: GENIE Project AAV-mediated expression of improved GABA sensor control (no change in fluorescence) In-stock AAV preps available in AAV1 and AAV5; other serotypes available on demand. https://twp.ai/4it0eQ

We’re looking forward to the Society for Neuroscience (@sfn.org) conference in San Diego next week! Our team will be catching talks on everything from neuronal cell types to pathways of neurodevelopment and degeneration — and we’d love to connect while we’re there. Here’s where to find us:

Come discuss antibodies with @ipiproteins.bsky.social at #SfN25! Join us Monday, Nov 17 (11–3) in booth # 3625 for IPI office hours. Let’s chat about new tools for axon guidance & the synaptic cleft — and what antibodies you want to see next.

Thanks for sharing your insight with the community!

Glypicans, complete! We now have recombinant antibodies targeting GPC1–6, giving researchers full coverage of this key family. Thanks to our partners at IPI! Extensive characterization available: https://twp.ai/4ispyb

New from the Hirokazu Hirai lab (Cell Rep Methods, 2025): an AAV vector engineered for specific and efficient gene expression in microglia. https://twp.ai/4ispvR

Are you presenting research at #SfN25 that uses materials available through Addgene? Join our Addgene Ambassadors program! Learn more: https://twp.ai/4ispwV