Here's a six-month time-lapse of me building the single objective light sheet fluorescence microscope at the UCSF Center for Advanced Light Microscopy! Also pictured is @nicostuurman.bsky.social and @kahsage.bsky.social. #snouty

Some Micro-Manager device adapters are not included in the installer. That can cause problems when the device API changes. When the device adapter vendor does not release a new version you can try the following work-around: nicost.github.io/microscope-t...

Here's one of the incredible dataviz images that Ron Milo just showed at #EMBOclimateResilience, from the excellent Greenspan et al PNAS paper www.pnas.org/doi/epub/10....

Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.

Our openSPIM is officially up and running to image the latest catch of the day! Fueled by pymmcore-plus & pymmcore-gui! 💪⚡🔬 pymmcore-plus.github.io/pymmcore-plus/ github.com/pymmcore-plu...

Just had the pleasure to experiment with a camera targeting the astronomy market, but offering great value for microscopy. Cooled, low noise (<1.5 e read-noise), high QE cameras that work with Micro-Manager can be bought for ~$1k: nicost.github.io/microscope-t...

How do tiny magnetic fields affect biochemistry? Overflowing with pride over Katherine Xiang's study on a giant magnetic field effect in a red fluorescent protein, www.biorxiv.org/content/10.1...

PREPRINT OUT ✨ "hack" your microscope to work as a 3D printer! print micrometer sized features at cm scale. a method for easy and cheap microfabrication, with a focus on biological applications. THREAD ↓ www.biorxiv.org/content/10.1...

Microscopy Nodes is now up on bioRxiv! 🚀 This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev). High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬 www.biorxiv.org/content/10.1...

A highly photostable monomeric red fluorescent protein www.researchsquare.com/article/rs-5...

Progress on the quest for a red mStayGold! Just out: mScarlet3-S2, a new photostable red FP derived from mScarlet3: www.researchsquare.com/article/rs-5...

We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇 www.biorxiv.org/content/10.1...

Advances in microscopy mean we can now do more than just observe biology—we can control it. But how far can we really push this in mammalian cells with all their (beautiful but annoying) heterogeneity? 🧪🔬(🧵)

“We don’t value software, data, and methods in the same way we value papers, even though those resources empower millions of scientists” 💯 www.statnews.com/sponsor/2024...

We designed MS2 and PP7 binding coat proteins that are degraded in cells except when bound by their hairpin RNAs. You can check out our pre-print here: tinyurl.com/27p7hyt9, and listen to a Google AI-generated podcast on it here: notebooklm.google.com/notebook/d71...

If I can add to the his-tag data: at least 6 consecutive his-residues target proteins to nuclear speckles: journals.plos.org/plosgenetics... So this could drive condensation in nuclear speckles.

Hi #biabob users, there comes a new edit mode with the new 0.24.0 release: If you ask %%bob to modify code, it will update the current notebook cell. This should make AI-assisted iterative code modifications much smoother. 🤓 github.com/haesleinhuep... [video shortened; Bob needs ~10 sec to respond]

New Dell laptop decided to install Windows sideways. Rotating it will flip the screen, but impossible to get it back to normal orientation. Hope this will straighten out.

Check out "ONE" Microscopy, which can achieve protein level resolution with a light microscope! I've been trying to combine Magnify + STED to visualize protein complexes. Looks like they were able to do it with X10 + SRFF ;-)