#plantscience question. Anyone know why the Tet-On (tetracycline/dox) inducible expression system never took off in plants?

In case anyone needs it for their syllabi, my statement in gen-Ai from the minicomic I made as a syllabus for class last semester. All online and printable here spinweaveandcut.com/fall-2025-sy...

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026

Stoked to have helped get this out the door! If you want to inject lots of mRNA into your embryos for higher SnR or to multiplex or to have it last longer… this is the way!

Structure, Function and Dynamics of mCoral, a pH-Responsive Engineered Variant of the mCherry Fluorescent Protein with Improved Hydrogen Peroxide Tolerance www.mdpi.com/1422-0067/27...

One of the most pleasant surprises of the past 20 years was the transformation of Wikipedia into arguably the most reliable general information source freely accessible to the public. It’s not perfect — I’ve certainly seen errors — but it’s kind of shockingly good. One might consider a donation.

We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.

Just stumbled on Mole, a MacOS command-line software that's great for some laptop end-of-the-year cleaning! Free an open source: github.com/tw93/Mole

One of the difficulties in developing code for microscopes is to get access to the equipment. So, wouldn't it be nice to have emulators? Turns out that AI is really good at converting documentation into an emulator: nicost.github.io/microscope-t...

🔬📊 PhD (100%) – Statistics & ML for self-driving microscopy Joint PhD with David Ginsbourger (Stats) & Pertz Lab (Cell Biology). Gaussian Processes, Bayesian design, active learning on live-cell experiments. ohws.prospective.ch/public/v1/jo... #PhD #Statistics #MachineLearning #Bayesian

💚 Sharing makes our future as bright as these biosensors 💚

I have an announcement to make. It's huuuge.....

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

"for" or "against" information overload?

Doing a PhD is - at heart - one long discussion with your mentor. The discussion changes over time - with unexpected turns and ups & downs - but through it all is a pair of people discussing a topic endlessly to make sense of it. PhD students: choose someone you like to talk to!

teach students `git` early todd.gureckislab.org/2025/12/19/g...

The 'Version of Record' of our paper came out on #FluorescenceFriday elifesciences.org/articles/105...

The (scientific) news I've been waiting for: www.nature.com/articles/s41...

Blue light decreases ER Ca²⁺ in stomatal guard cells, with refilling in the dark. Our new @newphyt.bsky.social study shows how ER Ca²⁺ shapes cytosolic Ca²⁺ dynamics doi.org/10.1111/nph....

We wrote two reviews on cell migration, published today in Nature Methods. They provides practical guidelines how to select a cell-migration assay and how to analyze cell-migration data Review 1: nature.com/articles/s41... Review 2: nature.com/articles/s41...

The obsession with a print-era portable format (PDF) is the only reason we have figures like this. Makes no sense on the Web. 1/2

who has favorite ways to measure hypoxia in tissues using immunofluorescence/microscopy? i guess maybe the gold standard is pimonidazole/hypoxyprobe- is there any reliable way that doesn't involve injecting anything in vivo? (like what do people do for human tissues?)

My "News & views" was published today @natmethods.nature.com Thanks @ritastrack.bsky.social for the invitation to write it and Allison Doerr for the editing after Rita left... www.nature.com/articles/s41...

Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

Save me

Shot in the dark, but has anyone tried transfecting EOL-1 cells (using lentiviruses, electroporation, or otherwise) and succeeded? We’re having some issues with these cells but it seems to be very vector-specific. Would appreciate any insight/reposts.

Just decline the peer review invitation. What are you people even doing?

Oh where, oh where does my protein localize? Oh where, oh where can it be?🎵 Check out our Subcellular Localization Collection page, with fluorescent protein markers for organelles and cellular structures to help you find your way 🔬

Research questions generated by AI. Questionnaires filled out by AI. Paper written by AI. Paper reviewed by AI. Just make it stop.

New preprint🚨 Imagine (re)designing a protein via inverse folding. AF2 predicts the designed sequence to a structure with pLDDT 94 & you get 1.8 Å RMSD to the input. Perfect design? What if I told u that the structure has 4 solvent-exposed Trp and 3 Pro where a Gly should be? Why to be wary🧵👇

One of the most exciting outcomes was that we figured out how to control two different proteins independently in the same living animal, a “dual channel” AID system that opens the door to cool new experimental designs. 8/10

New paper from @zhixingchen2.bsky.social's lab! It turns out that, in addition to its very low phototoxicity, PKmito Deep Red (PKMDR) directly reports on mitochondrial membrane potential (MMP) in live cells through its lifetime! www.nature.com/articles/s41...

I wrote a lil post on the amazing work that @ginareynolds.bsky.social does championing ggplot2 extension developers and teaching others to build their own! The post features the Scrollytelling Quarto extension and the group's cute #RStats hex 🐱: rworks.dev/posts/ggplot...

New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos. www.nature.com/articles/s42...

"But ChatGPT said..." stopcitingai.com

If you like this thread, it was written with my own brain, that bag of meat in my noggin, transmitted by black magic to my fingers and to your screen. I felt things when I was writing it and I hope you did too. Machines can’t do that

Dear Bsky #imaging friends, what would you recommend to learn how to do cryo-EM? (from sample prep to analysis) What is/are 'THE' lab/ facility/ trainings/ conferences you recommend me to visit/attend? I am open to anything and Budget is not an issue. Bonus if they are #ribosome specialists. 🤓 🔬

Interesting paper by the groups of @zhizhou1619.bsky.social and Xiang Sheng in @natcomms.nature.com. They developed a system, with which thiophenols can be fed to cells, converted into non-canonical amino acids and used for genetic code expansion in protein engineering.

Wait, Mastodon wasn't published yet???????? @jytinevez.bsky.social @paveltomancak.bsky.social

A glittery, festive #FluorescenceFriday today, spent playing with some fun samples 🪩🕺

GPT 5.2 is now included in our R code generation eval! The -Pro version is slightly SoTA at a substantially higher price point than similar performers. Read more: skaltman-model-eval-app.share.connect.posit.cloud

We salute you, Charles Clyde Ebbets

A reminder to use the right P2A sequence to produce separate proteins - the images are from a GPCR-P2A-mCherry construct using a P2A variant without (upper row) and with (lower row) a preceding GSG linker.

I ignored the strip.clip argument in #ggplot2 for way too long 😲 Combined with a small negative margin tweak, you can place facet labels inside each panel. A tiny trick that makes small multiples feel so much cleaner. 🔵 no manual coordinates 🔵 inherits theme styling 🔵 scales nicely when resizing

Working on this right now for our revisions! Most important question though, what's your favourite colour to use for highlighting changes?

Come and work with us, We are looking for a new group leader at Turku Bioscience @turkubioscience.bsky.social. Åbo Akademi University invites applications for a fixed term position as Head of Research in Bioscience (Principle Investigator). abo.rekrytointi.com/paikat/?o=A_...

HELP! Please suggest a cool paper/preprint in your field in the last year or so. For a course I teach, I am collecting papers on: cell/dev bio, microbio, neuro, maths/stats/CS/chem/phys applied to biology. 1/2

"there's a new serif in town"

Was playing around with ThermoMPNN and decided to reproduce the resulting heatmap with R/ggplot2 - this is added as dataViz Protocol 28. All #dataViz protocols available here: joachimgoedhart.github.io/DataViz-prot...

Back in 2016, I spent some time centrifuging chambers containing a kinesin/microtubule -based active gel so that all the material ended up on one side of the chamber. Then recorded how long it took to expand.