You may like this piece by @matti.vuorre.com then: universonline.nl/nieuws/2025/...

Question from Slack: Looking for antibodies against mscarlet-i that people have good experience with? #AskZebrafish

I'm worried about outsourcing to a machine something that's part of our common endeavour to figure out the world. It's not enough that stuff is figured out, it's about **us** figuring it out! I don't remember phone numbers anymore – I outsourced that to my phone. I don't want that for my science.

Spoiler alert: Ensure that it works ‘out of the box’

Let's go! Super excited to partner with @richardsever.bsky.social and the fantastic @openrxiv.bsky.social team - Preprints have been the best thing that happened to science publishing during my lifetime, and we're happy @qedscience.bsky.social can make preprints do more 👊

The Lab of Stephan Grill at MPI-CBG has finally made it to Bluesky and we also have a brand new homepage: grill-lab.org Check it out.

AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...

Am looking for a cloud storage solution that is European / non-US - anyone experience with jottacloud? Other recommendations? jottacloud.com/en/

I'm thrilled to finally share my PhD work on understanding how Ca2+ signals regulate the protruding front of the cell. It’s been an incredible journey working alongside all the collaborators. www.biorxiv.org/content/10.1...

Please repost - Multiple PhDs available www.findaphd.com/phds/project... www.findaphd.com/phds/project... www.findaphd.com/phds/project...

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site #microscopy #ImageJ #FluorescenceFriday #microscopyMonday imagej.net/plugins/imag...

HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social

Required reading for cell biologists to get a sense of basic statistical principles! www.nature.com/articles/s41...

Tomorrow morning! Come by!

The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it! www.fpbase.org/spectra/

I'd only dabbled with napari, but needed to use it for a paper we're writing. This is a quick post about getting started: from installation to a folder full of thousands of processed images, with gotchas along the way. quantixed.org/2025/11/04/a...

Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects www.biophysics-reports.org/article/doi/...

Who did this?!

Am looking for a cloud storage solution that is European / non-US - anyone experience with jottacloud? Other recommendations? jottacloud.com/en/

Not having to use Teams, Sharepoint, MS365 could be a big competitive advantage for collaborative research teams.

HaloTrace: A spatiotemporally precise fluorescent readout of blood-brain barrier permeability in mice by Chenghua Gu and team: www.biorxiv.org/content/10.1...

In which we all learn the phrase “ballistic microscopy”:

🧵Remember the preprint claiming SARS-CoV-2 has a "synthetic fingerprint"? It said a restriction map was "extremely unlikely to have arisen by random evolution.” I took its claims seriously. The "synthetic fingerprint" hypothesis collapses. My new preprint explains why. arxiv.org/abs/2510.23833

A most surprising world map came by this night - it was correctly categorized by Pub quiz team member @andreacaldarola.bsky.social

Python: The Documentary. A fascinating watch, I think even for non-coders (or the code-curious). Watched this last night and both enjoyed it and learned some new context to my programming! Apologies that I lost the info of whoever on here I saw post this first. www.youtube.com/watch?v=GfH4...

A class like no other!! From #AI structure hallucination 🤖 to #CryoEM structure reality 🔬 by @biozentrum.unibas.ch @unibas.ch undergraduate students👩‍🔬👩‍🎓 in just a few weeks! One of their creations is this beautiful flower-shaped tetrameric pore 🌼, a brand new member of the #ProteinCosmos 🧶🧬 🧪

I am genuinely impressed by large language models - they can absorb disparate components of text into some consolidated view, they can produce extremely good language and - with the right model - translate pretty well between languages and they are an excellent text based UI for humans to use. But..

How can we see the cells that make up a living organism? Membrane-localising tags can drive fluorescent proteins to the cell's outer membrane, making their outlines visible. But the tags don't work well in all organisms. How do you find one for your species of interest? 🧵 Check our latest preprint

mStayRose is published in JBC 🥳🎉 Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable: www.jbc.org/article/S002...

🧠 Even in the age of AI, classical image filters still matter! They reduce noise, preserve edges, and boost segmentation + ML performance. Filter smart — shape what your algorithm sees. 🔗 Read more: “To filter, or not to filter?” #ImageAnalysis #AI #Microscopy #Segmentation bit.ly/46mYBL7

I wrote an updated guide on which AIs to use right now, & some tips on how to use them (and how to avoid falling into some common traps) A lot has changed since I last wrote a guide like this in the spring, and AI has gotten much more useful as a result. open.substack.com/pub/oneusefu...

29 oktober: stem vóór de democratie.

Reminder of the importance of negative results for research quality and integrity, published in @plosbiology.org @lsajournal.org will gladly consider your manuscript with negative or confirmatory results! Read the full piece here: doi.org/10.1371/jour...

💡 Networking can often feel like a daunting task, especially for those who are new to professional events. Here are some engaging strategies to help you flip the script... 👉 ulrikeboehm.org/networking-r...

Have a nice #FluorescenceFriday! This is pollen from pine tree, imaged from auto-fluorescence by confocal microscopy the LUT is "KTZ bw DarkGold" 🔥

For #FluorescenceFriday - human iPSC-derived cerebral organoid - labeled w/ MAP2🟢, CTIP2🔴, PAX6⚪, Hoechst🔵 from our Molecular Neurogenetics team. Image credit to @tsawada1.bsky.social and JHU Human Genetics grad student Bailey Spiegelberg 🔬🧠🧪

Really amazing initiative from @odedrechavi.bsky.social & Co at @qedscience.bsky.social: an AI tool to critically evaluate your work & get constructive feedback to help you assess/improve it. Read the whole 🧵fo details & website link 👏👍👇

"The robust analysis of the 1748 confocal stacks issued from the RNAi screen of 103 RNAi lines targeting 64 GAPs and GEFs would have not been possible without the development of a written Python program." journals.biologists.com/jcs/article/...

No more than two pages, but one should usually do the trick. At Nature Portfolio the cover letters get read. Use bolded text sparingly. Use plain language to convey what's been done, why the study is cool, why it is timely, and what the broader implications might be.

I regularly get derailed when someone tries to benchmark clustering methods, trying the find the "best" one or the one that agrees with "ground truth". Which clustering of the below animals is the best or the true one? By row, or by column?

BIG ANNOUNCEMENT📣: I haven’t been this excited to be part of something new in 15 years… Thrilled to reveal the passion project I’ve been working on for the past year and a half!🙀🥳 (thread 👇)

Any suggestion on a company that does synthesis for ~200-bp fragments that good pricing? Twist is 300-bp min.

Dear funding agencies, I know we all want to discover the wonder drug that will cure the horrible diseases, but to do that, we need to invest in basic, unsexy, foundational research on how the systems work. Funding can’t all be drug development. Sincerely, Looking for basic research grants.

Periodic reminder that the fluorescent compound in mammalian cell culture media, such as DMEM, is riboflavin (not phenol red)

Pleased to share our review on “Synthetic Mechanotransduction” — now live at Nature Reviews Bioengineering. May the force be with you! www.nature.com/articles/s44...

When you look at animal & plant cells remember what they give rise to... mind blowing stuff! Find out more about our paper models here: buff.ly/fqKSYnW #EduSky #plantcell #animalcell #cells #iteachbio #scienceteacher #biologyteacher