Quantitative Imaging: From Acquisition to Analysis (QICM), April 6 - April 21, 2026 Thank you to all of the participants and vendors that supported the course! #cshlcourses #cshl Photo Credit: Max Brambach, Eva de la Serna, and Federico Gasparoli (2026 Teaching Assistants) @qiatcshl.bsky.social

So thrilled to be part of Quantitative Imaging @qiatcshl.bsky.social Such an amazing course and now we got a whole new generation of expansion microscopy enthusiasts!

@lycasworks.bsky.social showing expansion microscopy followed by some hands on expansion exercises.

Hari Shroff giving the QI keynote on structural illumination microscopy, adaptive optics and new biosensors. Wow!

Super Res Sunday! And we are doing all the techniques:)

Light sheet lab. We image anything from cells to plants to shrimp. @fedegasparoli.bsky.social @excitedemissions.bsky.social are running the machines!

Light Sheet Saturday is here. @jencwaters.bsky.social and @talley.codes lecture on how to improve your photon budget by illuminating from the side.

Pizza and image analysis. Because there’s no hackin‘ without snackin‘. @damiandn.bsky.social @bethcimini.bsky.social

AI for image analysis. @florianjug.bsky.social @bethcimini.bsky.social @damiandn.bsky.social are showing off the power of the machines:)

Cake and image analysis. True QI bliss:) @damiandn.bsky.social @bethcimini.bsky.social @emiglietta.bsky.social teaching 3D segmentation.

Looking for samples for our lightsheet microscope and expansion microscopy lab, @fedegasparoli.bsky.social and @lycasworks.bsky.social go on an expedition during low tide.

Confocal lab is where we show off the power of optical sectioning. Only one question remains: point scanner or spinning disc?

Hard to decide what’s better the beautiful weather or the lectures by @talley.codes and @jencwaters.bsky.social on confocal microscopy. (it’s obviously the lectures)

Hands on TIRF microscopy and machine learning segmentation has students up all night.

We are getting serious about analysis. Today Hunter Elliott and @florianjug.bsky.social introduce machine learning and AI. It’s not magic, it’s computers.

Today’s lab was all about live cell microscopy.

Today’s lectures on live cell imaging and fluorescent proteins by @jencwaters.bsky.social and @talley.codes Every photon counts!

Today at @qiatcshl.bsky.social , @emiglietta.bsky.social teaching the good word of CellProfiler!

Late night coding and microscopy today!

We tried other brands but nothing works quite like a Guinness for our light sheet microscope. Future collab?

Today @bethcimini.bsky.social and @damiandn.bsky.social lectured on image correction and colocalization. Key take-home: The computer can’t do magic; if you don’t collect photons at the microscope you can’t make them appear later.

What a day! After briefly enjoying the beautiful weather we went back into our dark microscope rooms and learned more about SNR and the different sources of noise in images. Did you know you can report intensity values as photons instead of arbitrary units?

Today Hunter Elliott is lecturing on image processing. Key take-home before lunch: There is no free lunch:)

This is me!! Currently in the QI course at CSHL… 🔬💡 It’s been 3 days (or 2 weeks??) and I’ve learned that NA means more than “not available,” that Köhler was very into conjugate planes, and that my eyes suck at a level I didn’t know was possible. Very excited for what's coming!! 👩‍💻

Today’s labs on digital imaging! sCMOS? CCD? So much more!

@talley.codes teaching about digital cameras. Remember Airy disks?

Learning about the components of a microscope. How are images formed and what is that back focal plane everyone seems so excited about?

The magic of polarisation.

This morning‘s lecture is about Koehler illumination. Pop quiz: What happens when you close the condenser aperture?

Cleaning objectives is a very important skill to learn! @fedegasparoli.bsky.social showing how it’s done:)

The famous ‚fluorescence depends on the environment‘ lecture! Need more light? Consider adding base to your sample:)

We are back! @jencwaters.bsky.social giving the first lecture of 2026 QI at CSHL. Two weeks of intense microscopy training ahead.

🔬 🖥️ 📊!

Don't let the price of QI deter you! QI offers financial aid through NCI, HHMS, and @bioimagingna.bsky.social. Include a brief statement of need with your application. Aid is distributed by CSHL (not the course instructors). Many of our students - sometimes all! - receive significant support.

Applications for QI 2026 are due on Friday! 🔬 🖥️ 📊 😺

💻 Apply to our beginner-friendly Boston Bioimage Analysis Course! Get started with Python and learn to analyze fluorescence microscopy images — no coding experience required! Fee waivers available thanks to funding from @bioimagingna.bsky.social! 🗓️ Apply June 1st! iac.hms.harvard.edu/bobiac/2025/

‼BREAKING‼ We’ve secured new funding for the Boston Bioimage Analysis Course (BoBiAC) this July at Harvard Med School! Course fees down 37%, with fee waivers for academic applicants, for an intensive hands-on intro to bioimage analysis with Python! Apply by June 1st! iac.hms.harvard.edu/bobiac/2025

Slick t-shirt design for @qiatcshl.bsky.social made by @sciencedoodles.bsky.social featuring fan favorite the bench scope

Happy Expansion Microscopy Day at Quantitative Imaging @qiatcshl.bsky.social

Our latest preprint www.biorxiv.org/content/10.1..., describes Willi Stepp’s project to make smart microscopy even gentler by doing event detection in phase contrast. We developed neural networks to detect mito-LD and mito-lysosome contacts, as well as mitochondrial pre-fission constrictions.

Light sheet baby!! QI2025

Congratulations to students Jana & Michal for winning the Stayin' Alive challenge: acquiring a live cell time lapse with minimal photodamage! #stayinalive

Celebrating the last analysis lab at @qiatcshl.bsky.social in style. Friday night disco music (🤮) and bioimage analysis (😃)

Our first prize winners for their confocal data presentation: 'Pinhole Pros' Federico & Naly!

Analysis team cooking up some cellprofiler magic at @qiatcshl.bsky.social

Congratulations to students @nancypaniagua.bsky.social & Michal for placing 1st & 2nd place in our @ilastik-team.bsky.social pixel classifier challenge to segment with the least amount of annotations! #lazyannotationsftw

The students (totally) loved our TIRF lab! Check out this fluorescein solution sample we used to visualize internal reflection on the scope - 📸 by student Augusto Berrocal

Week 2! 🔬🖥️📊

Our image analysis expert instructors, @bethcimini.bsky.social, @damiandn.bsky.social, @florianjug.bsky.social & Hunter Elliott, are in full swing!

It's TIRF time again at QI!!! 🎉 🔬Check out @jencwaters.bsky.social's infamous TIRF demo to understand the principle behind total internal reflection vs refraction! #gonetirfing #criticallyawesome