It is out! 🦚 I recorded hundreds of #ExM 🔬images of cytokinetic bridges and averaged them into 6 stages. How? With help from our fantastic collaborators @zacsimile.bsky.social and @jonasries.bsky.social in Vienna 😇. Check out the full atlas here: doi.org/10.5281/zenodo.17232370

Now out on bioRxiv. 🥳My research on #cytokinesis, averaging thousands of #ExM images🔬, creating a dynamic atlas of cytokinesis 🦠⏳. Here's an animated sneak peek of what we found. Better resolution on bioRxiv😄 #PSFoftheGIF

Hello! Very excited to share our latest preprint, which is great news for us but terrible news for any diehard fans of PSNR and SSIM as image quality metrics in microscopy... (1/5) www.biorxiv.org/content/10.1...

Automated optogenetic control of hundreds of cells in parallel. Each cell is individually steered, collectively acting as a "tissue printer". Preprint & code out! www.biorxiv.org/content/10.1...

We’re excited to share LiteLoc — a lightweight and scalable deep learning framework for high-throughput single-molecule localization microscopy, enabling analysis speed of >500 MB/s on 8× RTX 4090 GPUs without compromising accuracy. rdcu.be/eztp6

🚨 2 × PhD positions @EPFL! 🚨 Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → [email protected] #PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT

Human scientists anticipate human reviewers will use machines, so they put tiny white text to prompt machines to give positive reviews. I’m not even angry, I’m impressed www.nature.com/articles/d41... Scientists hide messages in papers to game AI peer review

Good news I've fixed the worst thing about Europe

New preprint by @kjohnsson.bsky.social lab! A new split Halotag system with higher affinity of the complements + lower background. The system works with our SiR-CA & CPY-CA halotag ligands and enables STED imaging or FLIM multiplexing. The short 14 aa tag Hpep enables easy cloning free CRISPR-KI.

🚨🔬💗Whether investigating cell organelles or mapping proteins, together with Victor Puelles's lab we lay a roadmap for selecting optimal #ExM and #SuperResolution #microscopy combinations. Daria Aristova and Dominik Kylies review with amazing co-authors pubs.aip.org/aip/apr/arti...

Tiny yellow bird waiting at the metro stop this morning

New work from Qiuqiang Zhan. Nonlinear multiphoton excitation eliminates side lobes in 4Pi, achieves 26 nm axial resolution: www.nature.com/articles/s41...

Your yearly reminder to acknowledge the core facilities you use and their staff scientists in your papers. These scientists are a crucial part of the scientific ecosystem and to continue to exist they need tangible credit for their work. Plus their associated expertise adds credibility to your work.

We have a new review out! In this Microscopy & Microanalysis piece, Louisa Mezache and I survey some of the latest commercially available microscope technologies: Nikon NSPARC, Re-scan Gaia, CSR Biotech MI-SIM, refinements to STED, SMLM from Bruker and Abbelight… Check it out with free access:

Check out our new MINFLUX Simulator: www.biorxiv.org/content/10.1... with @zacsimile.bsky.social. Realistic simulation of optics, mechanics, fluorophore dynamics and estimators. @abberior.rocks users can directly simulate sequence files.

MINFLUX works well in theory, but system and sample imperfections can lead to suboptimal resolution. To address this, @jonasries.bsky.social and I developed SimuFLUX: www.biorxiv.org/content/10.1..., a realistic simulator to optimize MINFLUX experiments before samples go on the microscope.

Our manuscript on accelerating the acquisition rate in oblique plane microscopy (OPM) is online now: opg.optica.org/boe/fulltext... Our work addresses the issue that an OPM PSF is tilted, and as such it leaves a lot of "empty space" in Fourier space (FFT of experimental data on the right)... 1/n

Checking out the cherry blossoms at Votivkirche

Incredible 4Pi and pan-expansion imaging of the Golgi 👇

Just received the email that my recent appointment to a BSC, which was supposed to be until June 2029, was terminated effective immediately.

This is probably one the most infuriating things I’ve read: removing women, gender minorities and POC as outside reviewers? How can they keep claiming that THEY are the ones protecting against discrimination?!? www.statnews.com/2025/03/24/n...

I was invited, for July, to a scienctific meeting in Telluride (Colorado). I was told it is a wonderful place for a conference, and I was very much looking forward to an exciting meeting in a fantastic environment. I have just canceled my participation, despite having booked already everything.

I’m excited to share our latest research from the Musser Lab, published today in Nature. Using two-color 3D MINFLUX, we directly visualized nuclear import and export events through transport-active nuclear pores. (1/5) www.nature.com/articles/s41...

#jobalert 🚨 We have 34 (!) open PhD positions @scadsai.bsky.social in Dresden and Leipzig (Germany). The cross-disciplinary topics range from NLP, KG, Protein design, Math foundations, Visualization, Ethics, ML, RL, DL, all things #AI. Join us! 💻🤖🚀 www.verw.tu-dresden.de/StellAus/dow...

By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...

Bluetorial: Women, courage, and leadership What follows will include some generalizations based on population averages of what I have experienced over the course of my career. There are, of course, exceptions in every group who are substantially more to one extreme or the other.

If you're interested in advanced 3D super-resolution microscopy then there is an exciting new job opportunity in TheLeeLab here in Cambridge. Happy to answer any questions! 🔬🧪🦠 www.jobs.cam.ac.uk/job/50547/

Looks like an interesting new style of conference.

I'm from the US and currently having a great time in a postdoc in Vienna. Happy to chat and share advice for anyone interested in coming to Austria. By the way, the Vienna BioCenter PhD program application cycle just started: training.vbc.ac.at/phd-program/.

Microscope objectives can be confusing, and detailed specs (or even basic properties) are often hard to find. Here's an introductory resource to help new builders and instrument designers with the basics. It's a work in progress so feedback is appreciated: amsikking.github.io/microscope_o...

We've just published a new scientific preprint that dives into the spatial glycome using super-resolution microscopy. Discover the critical role of sugars and take a look at our visualizations revealing their cell-wide presence. -> www.biorxiv.org/content/10.1... #dSTORM #SMLM #glycobiology

PREPRINT OUT ✨ "hack" your microscope to work as a 3D printer! print micrometer sized features at cm scale. a method for easy and cheap microfabrication, with a focus on biological applications. THREAD ↓ www.biorxiv.org/content/10.1...

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

Pretty sad. Just got the email that CZI is canceling the second round of Diversity Leadership Awards. Private industry will definitely not fill the hole that NIH and NSF are leaving. 😢💔

The self-driving multiscale microscope from @daetwylerstephan.bsky.social is out. It can image an entire zebrafish embryo longitudinally and follow a volume of interest over time with high resolution. We used it to study cancer-immune cell interactions. www.nature.com/articles/s41...

We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

"Indirect costs" just means paying the full cost of the research. That's it. Some costs are for one project, like lab equipment. Some are shared by many, like buildings and utilities. Grants that don't pay shared costs are a net negative. The fact that Harvard used to turn them down drives that home

This profile has been scrubbed from the site. Rose Ferreira grew up without reliable electricity, immigrated to NY, then survived homelessness and cancer on her way to NASA. She was so moved by images from the Webb telescope she wept. This is what’s at stake web.archive.org/web/20231206...

1. Today the NIH director issued a new directive slashing overhead rates to 15%. I want to provide some context on what that means and why it matters. grants.nih.gov/grants/guide...

I’m going to run a series of posts about the NIH/NSF/etc. troubles concurrently with my usual science posts. I can’t and won’t ignore the former, but I need the latter for mental equilibrium.

This is extremely disappointing. Pre-emptive compliance with with the new administration, and with one stroke of a pen **existing** funds are cut :( . I would have expected more of an organization that "funds people not projects".

The more I work with AI-generated code, the more I find there are slight, sinister bugs in the results. Everything runs, but side effects are baked into the core of these functions. My current take is that LLMs are a great way to write buggy code faster.

the fucking enforcement arm of the federal government is launching a war on integration an effort to push tens of millions of americans to the bottom of an arbitrary hierarchy of value and yeah, it pisses me off to see nominal allies say “oh yeah maybe they have a point”

I’ve unfortunately needed to add more material to my post from yesterday. The re-election of Donald Trump is looking like the biggest act of American self-destruction since the Civil War. Same link, new title and some updates.

The entire archive of CDC datasets can be found here. HUGE shoutout to data archivists- this work is important 👏🙌🏻 archive.org/details/2025...

I am very proud to advertise our latest publication, "Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers," in Adv. Materials: advanced.onlinelibrary.wiley.com/doi/full/10..... We propose to use T4 bacteriophages for calibrating 3D super-res fluorescence microscopy. 🧵

🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.

In 2019, the #mesoSPIM and #ctASLM #lightsheet #Microscopy papers appeared back-to-back in @naturemethods.bsky.social - now Jinlong Lin, @zacsimile.bsky.social & @kevin-dean.bsky.social & Co introduce the MCT-ASLM - a chimera of both for smart multiscale #imaging! www.biorxiv.org/content/10.1...

The Image & Optics Facility @ Institute of Science and Technology Austria is expanding it’s image analysis services and has a vacant position for a service oriented *Image Analysis Specialist* with a strong interest for data infrastructure organization🔬🧑‍💻📈 - sharing is much appreciated!