We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells. www.nature.com/articles/s41... rdcu.be/eQVxt

We're excited to announce the poster prize winners of #EESImaging 🔬 A special poster prize committee elected three winners: Alara Kiris, @lumasullo.bsky.social and @wanluzhang.bsky.social. Congratulations! Read on to learn about their research: s.embl.org/ees25-09post...

Now online: October 2025 Issue. - Focus Issue on #biosensing, - DNA moiré superlattices, - Sugars at Ångström-resolution, - Solid-state #nanopores, - Non-aqueous Li #batteries, - - Neuromorphic vision, - Peptide #hydrogels, - Deep learning for #LNPs and more... www.nature.com/nnano/volume...

Our article "Ångström-resolution imaging of cell-surface glycans" made it to the cover of Nature Nanotechnology! 🤩 @natnano.nature.com www.nature.com/nnano/volume...

Thrilled to announce some big news! Excited to share that I’ve been awarded a Wellcome Trust Early Career Award to establish my research as a Group Leader in the Glycosciences Program at the Institute of Biology and Experimental Medicine (IBYME) in Buenos Aires, Argentina, beginning in early 2026.

Within this neuronal atlas we can reveal the three synapse classes, excitatory, inhibitory and the recently discovered mixed synapse. Organelle imaging of Peroxisomes (Pmp70) and the Golgi Apparatus (Golga5) reveals rare contact sides and even fused particles. (5/6)

To show the power of the technique, we acquired a 13-plex 200 x 200 µm2 neuronal atlas in 3D. With this atlas we map the interaction architecture of three neurons, resolving organelles, cytoskeleton, vesicles and synapses at single-protein resolution. (4/6)

We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)

The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)

Highly efficient 12-color multiplexing with speed-optimized DNA-PAINT. We are excited to share our latest paper in @natcomms.nature.com, using left-handed DNA to extend speed-optimized DNA-PAINT to 12 targets in a simple and straightforward way! 🧬👈🚀https://www.nature.com/articles/s41467-025-64228-x

📣 📅 Invitation to two talks at our institute next Tuesday, October 7: 🤩 Luciano Masullo @lumasullo.bsky.social from @mpibiochem.bsky.social at 10 am, Adam Cohen @harvard.edu at 3pm, also online: us06web.zoom.us/webinar/regi... #maxplanck #mpimr #colloquium #medicalresearch #sciencetalks #science

Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field! www.nature.com/articles/s41...

Ångström-resolution imaging of cell-surface glycans Now published in Nature Nanotechnology! 🤩 @natnano.nature.com www.nature.com/articles/s41...

New paper online: Ångström-resolution imaging of cell-surface glycans. The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes. #Glycotime www.nature.com/articles/s41...

Congratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6) Read the full story here: www.nature.com/articles/s41...

Can we break past the usual limits of #SuperResolution #Microscopy—achieving deep-imaging, high-res, large FOV imaging without the trade-offs? 🧬🔬 YES: with #DNA_PAINT + Spinning Disk Confocal + Optical Photon Reassignment (#SDC-OPR)! 📄 www.nature.com/articles/s41... @natcomms.nature.com! 🧵👇

A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊 📄 bit.ly/4jTHfes

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩 We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution We are excited to present our latest work published in @natcomms.nature.com www.nature.com/articles/s41...

To look into: a Python alternative to ZEMAX... github.com/HarrisonKram...

I had a blast visiting the lab of Prof. Peng Xi at Peking University!! Prof. Xi and his team are doing amazing work on various type of SIM and other imaging modalities! Thanks so much for the warm welcome, the stimulating discussions, and the campus tour!!

Amazing visit to the lab of Prof. Wei Ji at the Institute of Biophysics (CAS) in Beijing today! They’re doing top-notch research, including awesome interferometric single-molecule localization methods (ROSE) thanks so much for the invitation, warm welcome and hospitality!! 😍🤩

#FOM2025 in Taiwan starting now!! Looking forward to great talks and also to tell about RESI and our latest work!

IMAGING LIGAND-RECEPTOR INTERACTIONS AT SINGLE-PROTEIN RESOLUTION WITH DNA-PAINT🔬 Ever wonder how cells "talk"? It starts when ligands bind to receptors on cell surfaces. We have cracked the challenge of imaging small ligands on cell surfaces. #DNAPAINT #celltalk 💬 doi.org/10.1002/smtd...

Check-out this powerful application of DNA-PAINT and RESI: imaging ligand-receptor interactions at molecular resolution! Project led by my extremely talented colleagues Monique Honsa, Larissa Heinze and Isabelle Pachmayr Very happy to have contributed to this beautiful work! 🤩

One month ago today, I published my first paper with the @jungmannlab.bsky.social 🥳 What better way to celebrate #MicroscopyMonday than with this STAR Protocol on SUM-PAINT spatial proteomic imaging: a guide for highly multiplexed DNA-PAINT imaging in neurons. star-protocols.cell.com/protocols/4066

An interesting preprint from the Horrocks' lab detailing the behavior of secondary nanobodies (such a @nanotag.bsky.social ones) at the single molecule level and optimizing protocols for multiplexed labeling: doi.org/10.1101/2025...

Mike spotted this before the rest of us. 🫡 Publications from 2025 are shared more on Bluesky than on X/Twitter.

To anyone who were excited for the release of SUM-PAINT, we now published a detailed protocol on to how replicate it in your hands! Check it out here: doi.org/10.1016/j.xp...

Excited to share the work of Jonathan Perr where he uncovered a surprisingly common feature of cell surfaces - the presentation and clustering of RNA binding proteins with #glycoRNA. Critically support by #NIH @cp-cell.bsky.social www.cell.com/cell/abstrac...

HIGH-RESOLUTION IN VIVO BRAIN IMAGING OF DROSOPHILA 🪰 New Preprint by Tassara et al.! www.biorxiv.org/content/10.1... #FluorescenceMonday #SnoutClub 1/6

Excellent resource for microscope builders! 🤓🪛🔬

Just a start! Who's missing? go.bsky.app/LAA7Sdx

I'm thrilled to share that our review article, "Molecular Level Super-Resolution Fluorescence Imaging," has now been published online (www.annualreviews.org/content/jour...)! We provide a comprehensive overview of fluorescence super-res methods that push the limits towards molecular-scale imaging.

We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🍬🍭🔬 For the first time, we’re able to study the molecular architecture of the glycocalyx at single-sugar resolution—a longstanding challenge in glycobiology! #glycotime Preprint and #bluetorial below!!

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

I am very proud to advertise our latest publication, "Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers," in Adv. Materials: advanced.onlinelibrary.wiley.com/doi/full/10..... We propose to use T4 bacteriophages for calibrating 3D super-res fluorescence microscopy. 🧵

🔬peeps! I am delighted to finally publish my code for controlling a spatial light modulator (SLM) to perform adaptive optics & to sculpt the vortex beams for STED & MINFLUX microscopy - complete w graphical user interface for easy use! github.com/wiebkejahr/s... Short user manual🧵

A journal club on @lumasullo.bsky.social et al. RASTMIN paper (with explanations for non-microscopists audience) www.youtube.com/watch?v=ozKA...

It's advanced talks day at #FOMISUR! Starting with @lumasullo.bsky.social demonstrating 30-color SUM-PAINT in neurons (doi.org/10.1016/j.ce...) as well as angstrom-resolution RESI (doi.org/10.1038/s415...)

Amazing talk by @christlet.bsky.social with stunning super-resolution images of neurons! 🤩 #FOMISUR

The #FOMISUR workshop has started! Joined the gang including @lumasullo.bsky.social for a local beer

#FOMISUR super-resolution microscopy workshop starts now! So happy to be here in my hometown Buenos Aires for this great event! Thanks to the organizers (Stefani Lab) for the invitation!

ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY. Can we do structural biology with an optical microscope? Yes! Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social [re-post from Twitter]

We are live at the @LMU_Muenchen Physics Science Fair! Come to our poster to learn about what we do at the @JungmannLab! We have several research projects for students at Bachelor and Master level!