Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

We introduce mirror-enhanced 4Pi-SMLM (me4Pi-SMLM), a streamlined single-objective configuration that leverages mirror-based retroreflection of the illumination beam to achieve near-isotropic localization precision of 2–3 nm, enabling unambiguous resolution of diverse ultrastructural features.

The cryo cyclical multiplexing expansion microscopy (Cy-ExM) preprint from @seweryn-galecki.bsky.social , @kevin-dean.bsky.social et al is online. 20 targets across an entire cell. Mr. Snouty also joined the fun. www.biorxiv.org/content/10.6...

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves near-isotropic resolution. @yongdengzhang.bsky.social www.nature.com/articles/s41...

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells. www.nature.com/articles/s41... rdcu.be/eQVxt

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. www.biorxiv.org/content/10.1...

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1... Read the thread below for a summary of our findings 🧵1/11

🚨 New Preprint! Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons. ▶️ www.biorxiv.org/content/10.1...

🚨 2 × PhD positions @EPFL! 🚨 Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → [email protected] #PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT

Excited to share our paper on ISM-FLUX, our new #MINFLUX-based technology. Achieve sub-10 nm localization uncertainty on single molecules using a straightforward #confocal-based setup with an array detector. 1/6 www.nature.com/articles/s41...

We present: Direct-view oblique plane microscopy www.biorxiv.org/content/10.1...

This is fantastic！

Incredible new work from Yongdeng Zhang demonstrating live 4Pi SIM--and in two colors! www.nature.com/articles/s41...