Yesterday I finally defended my PhD thesis - time to say thank you to all those who were part of this journey as mentors, collaborators, colleagues and friends!

Great work of @deglushk.bsky.social from my PhD lab @mpibp.bsky.social on how to get more information out of all the membranes you see in cryoET data!

We are looking for motivated #postdocs to join the lab! Biochemistry/microbiology/cryo-EM experience? Excited by #cryo-EM/ET, #chromatin? ❄️🧬🧫 Interdisciplinary projects, top EMBL facilities! Email CV + short cover letter → [email protected] Info about the group: www.embl.org/groups/dodon...

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️ Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

🔬 Applications are now open! Join MBL’s world-renowned Advanced Research Training Courses, where scientists from around the globe come together to explore new ideas, master cutting-edge techniques, and become part of a dynamic network. ➡️Apply today: go.mbl.edu/courses

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues. www.biorxiv.org/content/10.1...

What could be more exciting than watching Euplotes scurry around under the microscope? How about adding some raptorial predation by supergiant cannibal cells? www.biorxiv.org/content/10.1... Video by Vittorio Boscaro. 1/n

If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...

How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...! Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more! Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

At EMBL Grenoble, lead an independent research group using bold, original approaches to integrate complementary structural biology methods with emerging nano X-ray imaging technologies, bridging scales and modalities to unravel molecular mechanisms. embl.wd103.myworkdayjobs.com/en-US/EMBL/d...

The Molecular Systems Biology Unit is looking for an independent research group leader in one of many different areas from integrative structural biology to synthetic biology, and from AI-driven approaches to engineering biological systems. embl.wd103.myworkdayjobs.com/en-US/EMBL/d...

We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!! www.biorxiv.org/content/10.1...

The MPG is advertising directorships - powerful positions for which recruitment is usually started by them reaching out to potential candidates. Let’s hope this open call will get some great (but maybe overlooked) scientists on the candidate list and doesn’t accidentally select for oversized egos…

After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6

You are a scientist or an engineer (with a PhD) and you want to upskill to learn about delivering scientific infrastructure for the lifesciences (experimental or computational)? You want to work in Europe, in an internationally excellent service delivery team? ARISE2 is for you!

Beautiful cutting-edge in situ cryoET work (with very pretty figures) from the Grange lab! Great to see how the field is getting closer to doing structural work in tissues and how development in FIB technology, lift-out procedures, and correlative light microscopy contribute!

Hey optical tech dev folks: how do you do alignment? Can you outline your alignment procedures for positioning optics and verifying their correct positioning?

Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps https://www.biorxiv.org/content/10.1101/2025.05.27.656403v1

1. Can you figure out where I was standing when I took this picture? ChatGPT could. Given the photograph (scrubbed of all header information), the new chain of thought model, ChatGPT o.3, was able to pinpoint the location with a few meters.

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it. 🔗 www.pnas.org/doi/10.1073/...

Using the 'right tool for the job'. It's not fashionable, but a powerful and often underappreciated approach... -> Here's the yeast lifespan machine. Just a simple microscope with incubation, but full control over the hardware & software produced an excellent data platform! doi.org/10.1101/2022...

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

Cool idea form @svenklumpe.bsky.social to play with the FIB beam to achieve more efficient sample prep for in situ cryoET! Looking forward to seeing that in action!

Check out our recent preprint 👇on counting particles to estimate populations in #cryoem: noise can bias the estimates; with Luke Evans & a great team.

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Do you LOVE organelles?? Well I am THRILLED to announce that Nellie has been published in @naturemethods.bsky.social! Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy 🧵1/N

Such a cool story!!!

🚨🚨 MEGA JOB ALERT 🚨🚨 Independent Group Leader Positions in Computational Biology @humantechnopole.bsky.social! Are you ready to start your own lab? Do you know someone who is? Repost this + share with everyone who might want to know about it. Thanks!!! 🙏 More details below... check it out! 🧵 1/3

Exciting update on the CryoET Data Portal! A new annotation set from Mart Last & team is now live. They’ve doubled available annotations with >1,800 tomograms of Chlamydomonas reinhardtii which now have almost fully dense labels, and all can be visualized in the browser using Neuroglancer.

Applications for the MBL Physiology Course 2025 are still open. Apply if you want to spend your summer with an inspiring crowd exploring the many facets of cell biology (and beautiful Cape Cod)! My year was 2023, and it was a life-changing experience - went straight back for teaching in 2024…

Reminder that @arianebriegel.bsky.social compiled a nice list of women in #cryoEM . I often get asked to recommend people and this is a great place to start 😀 briegel-lab.org/women-in-cry...

We are hosting a cryo-EM grid nanofabrication workshop on 28 April 2025, as part of the Canadian Cryo-EM Nexus (ccemn.ca). Learn how to make holey gold or holey carbon grids in your own laboratory! Space is limited, so please apply at web.cvent.com/event/ee88ac...

Mahamid and Woodruff labs use cryo-ET to look at mitotic centrosomes: Stunned to see the pericentriolar matrix taking shape, and what seem to be ‚S size’ gamma-tubulin complex oligomers capping the end of 11-pf microtubules. www.cell.com/developmenta...

Exciting work by Martin Beck’s group @mpibp.bsky.social providing further evidence for nuclear pores responding to mechanical stress. The work includes some nice modeling estimating the fluid flow across nuclear pores as a function of (osmotic) pressure. www.cell.com/molecular-ce...

I’ll have more to say about this later, but please have a look, and I hope the #TeamTomo community finds good uses for those 1829 Chlamy tomograms 🧪🧶🧬🌾🔬

Jesus, I love Bluesky. The replies here kick ass…

SPACEtomo v1.2 is online! github.com/eisfabian/SP... - Improved GUIs for lamella detection, target selection and CLEM registration make it the best target selection workflow for PACEtomo on any kind of lamella sample. - Easier installation and configuration Any feedback is much appreciated! :)

Hi Bluesky community! 👋 Are you looking forward to 2025 as much as we are? 👀 We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look! ➡️ s.embl.org/poster-bl #EMBLEvents #molecularbiology #lifesciencetraining

Looking forward to this lineup of exciting talks at this pre-ASCB workshop on Friday! Thanks @manorlaboratory.bsky.social and @thevillalab.bsky.social for the chance to present my #cryoET work on #NPC asymmetry in the #Beck_Laboratory! #teamtomo