Super cool cryo-ET work from my colleague Xiaohan resolving a ton of ribosome assembly intermediates in the nuclei of human cells!

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo www.biorxiv.org/content/10.6...

We @embl.org wrote a short preprint about optimizing for maximum completeness in #cryoet analysis. We looked at template matching, VPP imaging, and tilt-series refinement parameters, and why averaging ribosomes helps even if you aren’t interested in their biology 🏭 www.biorxiv.org/content/10.6...

Really excellent work by the bluesky-less Marie and Sara +coauthors in our lab @embl.org! Check it out for cool combinations of light microscopy and #cryoET, and if you're interested in how yeasts reorganize structurally in response to stressors. #teamtomo

Congratulations, Julia Mahamid! Julia Mahamid receives the @dfg.de Leibniz Prize for her work on structural cell biology! #LeibnizPreis

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...! Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more! Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

New paper from my colleague Soheil in the Mahamid and @jonasries.bsky.social groups looking at factors influencing laser devitrification on frozen #cryoem samples. With a far-red fluorophore, all-gold support grids will be especially good for correlative cryo-SMLM and EM! doi.org/10.1016/j.js...

Are you into cryo-ET, in-cell discovery, protein translocation and folding or just cool integration of structural biology, proteomics and bioinformatics? Then see our new work: www.biorxiv.org/content/10.1... Thank you to all coauthors for your amazing work!