2026 will bring great tilt series alignments for the ribosome-deficient among us 🎆

Here is why friends don't let friends calculate FSC on denoised half-maps. I took EMD-17961, denoised the maps using Warp's Noise2Map, and calculated the FSC between various things. With noise gone from the half-maps, their consistency is no longer related to SSNR. Denoised map vs. model still works

Don't get me wrong: the original IsoNet was the coolest thing to happen in cryoET in at least a decade. But after the initial “wow” moment it's time to make this topic more sciency

I'm not superstitious but I never set my TV's volume to 13

I'm used to seeing driverless Waymos at this point, but seeing a Waymo with no driver *and* no passengers roll down a hill still gave me a moment of “OMG THE PARKING BRAKE FAILED” panic

Cool, definitely a visual improvement! But do I understand correctly the FSC was calculated between 2 *denoised* half-maps? You can't quantify resolution like that, only consistency.

😂

OMG they will go so well with my Bliss ugly Christmas sweater

It took me several weeks to notice how perfect this arrangement was

The things Apple will do to procrastinate improving Siri

Ugh, I'm starting to get spam texts with em dashes

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026

The North Bay is so packed with Porsches that the non-Porsches (Norsches?) are the real exotics there

Important to remember the theoretical size limit is based on assumptions and a model that may or may not be complete. It isn't some indisputable universal truth.

A fun introduction to problems arising from having to do Cartesian things on a sphere: www.bowerbyte.com/posts/blocky...

You're absolutely right!

K-Pop Demon Hunters OST playing on the radio? Cool!

I just found out the latest version of MacOS finally implements the motion cue dots iOS has had for a few years now, and it's doing wonders for me when I need to use my laptop on bus rides

I don't know what cursed token sequence puts Claude into stupid mode, but it's impossible to recover from it and you have to start a new conversation

First flight lesson survived!

Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.

I had a dream last night where some online store had the 5090 FE in stock at MSRP. It was a happy dream 🥹

He's currently beta-testing “faucet with a sensor that doesn't register your hands until you bring them in the least fortunate position, then sends water full-blast horizontally at them to be deflected to your crotch area to make it look like you peed yourself” in select locations

Y'all marketing people need to stop handing out reusable water bottles at every event

Came for the memes, stayed for the science

I've got bad news for #teamtomo: earlier today, our resident headcrab attacked @lorenzlamm.bsky.social, turning his Mem🧠 into 🧟‍♂️🧠

When you wake up at 4am because your spouse used the allyourblanketarebelongtous cheat code, and your brain puts on a slideshow to make sure you won't fall asleep again, but you need to make slides for a talk tomorrow so it's a literal slideshow

Been thinking of trying WarpTools but worried about having to start processing from scratch? @rasmusjensen.bsky.social wrote this handy guide for moving projects from desktop Warp to the latest version which runs on linux warpem.github.io/warp/referen...

Damn, this is brilliant. But I feel like the authors didn't answer the most important question: can you make tiny soap bubbles with this??

If Anthropic turns a profit this quarter, it will be mostly due to me burning tokens in Claude Code

Don't miss this workshop at a top-secret location in central London!

I must consider the possibility that I like Melbourne even more than Heidelberg

It's amazing how your brain instantly adjusts to everything being upside-down in Australia. But you can't fool a camera!

Hey Australians (and everyone familiar with the place)! I'll have a few days in Melbourne after #Tomo10 next week. Can you recommend anything fun to do/explore while I'm there?

I'm sorry, ChatGPT, but Claude and I are just happier together

De novo tilt series alignment accuracy is still the #1 problem, even with thinner samples. Can't recognize particles if they're mangled by misalignment.

Looks like way less to me 😕

People who set their car to make a loud beep upon locking: WHY???

Post a perfect album from the 90s that isn’t Nirvana, Pearl Jam, Soundgarden, or Alice in Chains.

Reading popular threads becomes easier once you add 📌 and 📍 to your list of muted words

I want to play the new Half-Life 2 anniversary edition, but I don't think I'm brave enough for another tour of Ravenholm now that I'm 20 years older

Whoa, now I have to scroll to get to the end of updates after leaving the app for half a day!

Is this place finally taking off?

Mr. Cat Executive Officer, am I in trouble?

@sjorsscheres.bsky.social, how about some T-shirts to get everyone to agree quickly on a tomography standard?

Any sane countries with decent science infrastructure left out there? Asking for a friend.

Avocado ice cream is surprisingly good!

Playing a Deutsche Bahn mission in Train Sim World 5, I let the ICE depart on time, thus immediately failing.

We really hit the trifecta of “kid in the unit upstairs”: hyperactive, heavy, homeschooled

Cat hack: put any toy in front of a tunnel exit to make the toy 10x more interesting