But there’s a curious loose end to this story. Scientists have been looking at PSII organization in thylakoids for over 50 years (including some really stunning freeze fracture 🔬😍). Sometimes PSII looks randomly oriented, and other times it forms extended 2D crystalline arrays in the membrane 💠 9/🧵

👀 this is really interesting!

PSA: 9 billion USD in research funds stolen by publishers over a 5 year period for open access fees - this takes away from your ability to pay your people what they are worth. Preprints forever.

This is the research we need 😋 I have been struggling for years to make reliable cacio e pepe, can't wait to try it out ista.ac.at/en/news/2025...

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching https://www.biorxiv.org/content/10.1101/2025.09.11.675606v1

Together with colleagues at the Dept. of Microsystems Engineering, we are looking for a PhD student to develop methods for femtoliter-scale sample dispensing in cryoEM sample preparation. edu.nl/h436k If you like interdisciplinary research & fascinating physics at ultrasmall scales, please apply!

First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...

Mitochondria finally made the headlines, yay! Oh wait, ... oh no ....

As teams grow, new Ph.D. graduates are less likely to land tenure-track jobs and more likely to leave science—especially women and international researchers, according to research from last year. https://scim.ag/3JHinJs #ScienceMagArchives

PVV-programma wil trans mensen erkenning ontnemen, docenten screenen op "politieke neutraliteit", XR en Antifa als "terroristisch" aanmerken en verbieden, "Alle Syriërs terug", uit EVRM en VN-Vluchtelingenverdrag, verbod op "links-liberale seksuele indoctrinatie". NOS: "er zijn scherpe randjes af".

It's hard to express how much gratitude I have to everyone involved in the journey, both mine and @napari.org's, that has led to this point. #EurekaPrizes A small attempt below: 👇

ASS is short for acephalic spermatozoa syndrome in which head and tail of sperm are detached⛓️‍💥, commonly caused by mutations in the gene encoding for SUN5. Using in situ cryo-ET❄️🔬 we find that SUN5 forms a protein lattice in the nuclear envelope (NE) at the head-tail junction of mammalian sperm.1/4

In our latest preprint on bioRxiv, we present the in situ structure of the human gap junction at 14 Å resolution using cryo-ET. The structure provides a blueprint for investigating how connexin regulation shapes intercellular communication in health and disease.

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

Exited to share my first PostDoc contribution! Like Magellan explored the globe, you can explore your cryoEM data with Magellon. Congrats to all involved. Scott Stagg Lab & Michael Cianfrocco Lab @landerlab.bsky.social www.biorxiv.org/content/10.1... 🌎❄️🔬

spent some time this evening updating the teamtomo.org site - if you're interested in cryo-EM + Python you might want to take a look around!

And it’s done! Thanks to everyone who came out for the first edition of FrostByte, our event for the local computational cryo-EMers in the Bay Area ❄️ Special thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing 🙏

Final PhD paper now reviewed and published in JSB:X. I was happy with some great reviews that improved the paper! Thanks to Sander Roet for his help with the code base, and Remco Veltkamp and @fridof.bsky.social

In the Bay Area? Love computational cryo-EM/ET? Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community! Details and registration at frostbyte-cryoem.github.io Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Okay hear me out, what if building your protein model was a first person horror survival experience?? #MolecularNodes #b3d #GeometryNodes 🧪

Looking forward to this dataformat standard ☺️ #CCPEM

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social, @marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social 🔗 Preprint here tinyurl.com/3a74uanv

(4/5) By combining in-situ #cryoet and #proteomics, we could assemble an integrative model, which illustrates the remarkable architecture of the amylosome - the complex that allows R. bromii to act as keystone degrader in the gut microbiome.

Honored to receive the Kluyver Award at the KNVM/NVMM Spring Scientific Meeting today!

Wat een serpent... Faber geeft geen goedkeuring voor lintjes COA-medewerkers - nos.nl/l/2561674

Utter disgrace. For those who missed it, these anti-protest laws were actually written by an oil industry funded lobby group. www.opendemocracy.net/en/dark-mone...

Insane

In 1968, my dad's high school physics teacher helped their class build a crude electron microscope. In this photo you can see the increased resolution between the optical and electron image. I found this while sifting through his things and wanted to share.

clean loaf this morning

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput www.biorxiv.org/content/10.1101/2025.03.11.642690v1 #cryoem

Center for Molecular Medicine at UMC Utrecht is looking for a Principal Investigator in the field of fundamental Cancer Biology! The vacancy states tenure track level, but also candidates with a more advanced career are very welcome to apply. www.werkenbijumcutrecht.nl/vacatures/we...

To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n

Interested in learning to process cryo-ET data in the latest version of Warp? I'll be running a workshop on April 22nd in central London, UK details+registration: forms.gle/FLDr3ZWtVGXs... Hope to see you there!

#AdventOfCode 2024 is almost done! But don't worry - the puzzles aren't going anywhere. In fact, there are hundreds of puzzles from past AoC events that are still available! adventofcode.com/events

Insanely nice animation! The sound threw me off a bit though😅 not used to hearing that with these types of videos

Good night everyone. Fourier Transform aficionados in particular. 🧪⚛️

The best technology🧪 for rapidly reducing #CO2 emissions!

Bluesky is now the #2 social app in the US, Canada, and #3 in the UK 🦋 On bsky, ~30% of y'all are posters compared to the 1% main characters on other platforms. Turns out there's more chattiness & connection when you have algorithmic choice 🤭

Finally got some time to properly test #RELION5 complete pipeline, starting from raw frames to STA. 22 tilt-series (from EMPIAR-11830), picking done with pytom, 14600 final particles, FSC 4.1-ish on the LSU (Nyquist bin1 3.8). Ofc it's a ribosome 😅 but now I can try with smthng more chlengin #CryoET

Excited that our paper on varying microtubule lattice spacing in cells is out! hubs.la/Q02SXVbD0

1/3 Our template matching package has changed name to pytom-match-pick (github.com/SBC-Utrecht/...) as it started diverging from the original project! It is now also on pypi, so you can easily install it with pip (thanks to twitterless sroet!)