Ever wondered how to position organelles in bacteria? Wonder no more! Happy to have contributed to this fantastic story from @cellforganized.bsky.social' lab. The avenues this opens in the synthetic biology space are enormous! Movie: Carboxysomes positioned in E. coli! Preprint: shorturl.at/D6xVg

I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

Endoplasmic reticulum-anchored nonstructural proteins drive human astrovirus replication organelle formation journals.plos.org/plospathogen...

The structure of the Human Adenovirus 7 virus-like particles reveals that pentons and core-genome promote hexon-pIIIa interactions during capsid maturation www.biorxiv.org/content/10.1101/2025.09.22.677823v1 #cryoem

Hepatitis C virus NS3/4A protease cleaves SPG20, a key regulator of lipid droplet turnover, to promote lipid droplet formation journals.asm.org/doi/10.1128/...

Rapid elicitation of a new class of neutralizing N332-glycan independent V3-glycan antibodies against HIV-1 in nonhuman primates pubmed.ncbi.nlm.nih.gov/40964314/ #cryoem

Part of something that we have been working on for a while-A 3.1 Å map of a flexibly tethered 38 kDa domain. Hopefully preprinted soon along with a few other structures. #CryoEM

Great to have this one published @cp-cellreports.bsky.social !! Some sarbecoviruses can use more ACE2 orthologs than others. The reason? A few sites in the RBD modulating the receptor usage of these viruses throughout their evolution! Worth a read 👇👇👇 www.cell.com/cell-reports...

This is an amazing paper led by the Grinter lab on bacterial protein antibiotics called pyocins and the mechanism by which they inhibit the BAM outer membrane complex. @rhyswg.bsky.social, @knottrna.bsky.social, @debnathghosal.bsky.social, @doylemt1.bsky.social, @fabianmunder.bsky.social

Interactions between the 2C protein of FMDV and components of the viral replication machinery are mediated by ER-derived membranes https://www.biorxiv.org/content/10.1101/2025.09.17.676763v1

Polyamine depletion inhibits norovirus infection by blocking virus-induced apoptosis https://www.biorxiv.org/content/10.1101/2025.09.14.676151v1

Coming up next week: the third Dutch Structural Biology meeting! Now in Delft, on Sept 26. See program here: nvbmb.kncv.nl/biology-2025 with #teamtomo #cryoEM #nmrchat simulations and more (looks like) Organized by the #NVBMB (associated with KNCV)

❄️ NEW PRE-PRINT ❄️ Happy to see our latest work online. Here we present a super simple solution to the preferred orientation problem in single particle cryo-EM: the use of ultrasonic excitation during vitrification! Details in the 🧵 below... #cryoEM #structuralbiology

Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem

Aminopeptidase N is a receptor for hedgehog merbecoviruses pubmed.ncbi.nlm.nih.gov/40950050/ #cryoem

In situ cryo-ET redefines HPV disassembly and transport paradigms pubmed.ncbi.nlm.nih.gov/40949969/ #cryoem

Structural Hijacking of FcRn by Human Astrovirus Spikes Reveals Conserved Epitopes for Broad-Spectrum Antivirals https://www.biorxiv.org/content/10.1101/2025.09.13.676064v1

Structural and Functional Hallmarks of Sindbis Virus Proteins: From Virion Architecture to Pathogenesis pubmed.ncbi.nlm.nih.gov/40943245/ #cryoem

Join us for the Austrian Cryo-EM Symposium Nov 11–12 at @istaresearch.bsky.social! Top speakers, cutting-edge cryo-EM, and a chance to explore Vienna & the ISTA campus. Register now 👉 cryoem-symposium.pages.ist.ac.at #cryoEM #teamtomo

Happy to share our lab's recent #cryoEM studies that show how a SPIN90 dimer activates Arp2/3 complex to form either uni or bidirectional actin filaments. Congrats🎉👏 @justusfrancis.bsky.social @achyutha-kp.bsky.social @tejashyamk.bsky.social @sridharskulkarni.bsky.social @kiranvyshnav.bsky.social

Partial Inhibition of Viral Replication Machinery Enhances Recombination in Herpes Simplex Viruses https://www.biorxiv.org/content/10.1101/2025.09.15.676234v1

Non-neutralizing antibodies to influenza A matrix-protein-2-ectodomain are broadly effective therapeutics and resistant to viral escape mutations www.science.org/doi/10.1126/...

In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria www.cell.com/cell-reports... #cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social

Featuring #AlphaBridge features from @alvarezsalmoral.bsky.social, with @jonasteuwen.bsky.social and team, our new work from @nkinl.bsky.social nl.bsky.social @oncodeinstitute.bsky.social . Have a look at ahmpc.eu for more!

Back from the 9th International Calicivirus Conference in Banff! Robin Veenstra and I really enjoyed both the stunning mountain views and the exciting science being shared in the field. Thanks to the organisers for putting together this great meeting. Lots of exciting collaborations ahead!

Genetically encoded FerriTag as a specific label for cryo-electron tomography pubmed.ncbi.nlm.nih.gov/40925364/ #cryoem

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

Well this is terrible… honestly the stuff I have to do for science - in Banff for the 9th international Calicivirus workshop.

Really excited to have been a small part of this work investigating sarbecovirus host range, receptor usage and antigenicity! www.biorxiv.org/content/10.1101/2025.09.03.673949v1

Structures of Nucleotide-Bound Redondovirus Rep Protein Link Conformation and Function pubmed.ncbi.nlm.nih.gov/40909492/ #cryoem

In situ cryo-ET redefines HPV disassembly and transport paradigms www.biorxiv.org/content/10.1...

Capturing the native structure of membrane proteins using vesicles pubmed.ncbi.nlm.nih.gov/40901875/ #cryoem

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

Phosphorylation toggles the SARS-CoV-2 nucleocapsid protein between two membrane-associated condensate states www.nature.com/articles/s41...

Hepatitis C virus NS3 helicase contributes to (−) strand RNA synthesis www.nature.com/articles/s41...

Exciting to see our protein binder design pipeline BindCraft published in its final form in @Nature ! This has been an amazing collaborative effort with Lennart, Christian, @sokrypton.org, Bruno and many other amazing lab members and collaborators. www.nature.com/articles/s41...

New paper: collaboration with Cambridge on EMCV 2B* now published journals.asm.org/doi/10.1128/...

PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms www.biorxiv.org/content/10.1101/2025.08.20.671250v1 #cryoem

Cryo-EM structure of the Seneca Valley virus A-particle and related structural states pubmed.ncbi.nlm.nih.gov/40833065/ #cryoem

Link to the publication from @utrechtvirology.bsky.social: nature.com/articles/s41...

A novel nanoparticle vaccine, based on S1-CTD, elicits robust protective immune responses against porcine deltacoronavirus | Journal of Virology journals.asm.org/doi/10.1128/...

Here's how to predict binding of tens or hundreds of small molecules to protein assemblies using Boltz 2 in ChimeraX. Accuracy depends on how similar the ligands and binding pockets are to existing experimental structures. www.rbvi.ucsf.edu/chimerax/dat...

📄 New paper: 3DEM Structure Map Validation Recommendations Two new metrics assess model-to-map fitness in cryo-EM: 🔹 Q_relative_all → compares avg Q-score across PDB/EMDB 🔹 Q_relative_resolution → compares within similar resolution #3DEM #CryoEM #wwPDB

Our preprint, in collaboration with Rino Rappuoli & Emanuele Andreano, is out! Co-led by colleagues at TLS, my grad student Ling Zhou @lingzhou.bsky.social, and postdoc Emily Rundlet @cryoemily.bsky.social, we identified OPG153 as a poxvirus bnAb target via B cell isolation & AlphaFold3 prediction.

Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryo-EM pubmed.ncbi.nlm.nih.gov/40815640/ #cryoem

New paper from @naturehealthglobal.bsky.social scientists identifies extensive spillover risk of an under-researched group of coronaviruses in China and Southeast Asia. 🧵 naturehealthglobal.org/new-paper-id...

Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone! www.nature.com/articles/s41...

Mechanistic insights into the small-molecule inhibition of influenza A virus entry pubmed.ncbi.nlm.nih.gov/40802690/ #cryoem

Signatures of omicron-like adaptation in early SARS-CoV-2 variants and chronic infection www.cell.com/cell-reports...

Cryo-EM protein structure without purification www.biorxiv.org/content/10.1101/2025.08.13.669967v1 #cryoem