A molecular-resolution look into the near-native architecture of the spinach chloroplast🌱. This one was a long time in the oven, but we're happy to finally share our "version of record". What long-standing debates did we settle? Check back for a short thread🧵 on Monday. #TeamTomo #PlantScience 🧪🧶🧬🔬🌾

How the twin-arginine translocase (Tat) system manages to transport folded proteins across membranes without any leaks? To answer this fundamental question we solved the first structure of TatB3C3 complex with bound cargo. Please check out new preprint! t.co/962Kj9pt6F

🚨🧠🔬 A major breakthrough in molecular neuroscience: I am excited to share a new story from our lab, published in accelerated format today by @nature.com: "Delta-type glutamate receptors are ligand-gated ion channels" Read more here (free article link): rdcu.be/eGIKz

We were one of the lucky ones to receive our NCI award. We have an open postdoctoral fellow or research tech position in the group. If you (or anyone you know) has strong expertise in structural biology or cell biology (genomic instability mechanisms) please reach out. www.antonylab.org

Structural studies by Ahmad Jomaa and colleagues @jomaalab.bsky.social show how NAC on translating #ribosomes couples protein synthesis to N-myristoylation of nascent polypeptides by NMT2 www.embopress.org/doi/full/10....

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social www.biorxiv.org/cgi/content/... @mrclmb.bsky.social @wellcometrust.bsky.social

Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️

First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...

Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...

Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social www.cell.com/cell/fulltex...

Our new review paper in stalled neuronal ribosomes with Wayne Sossin @mcgill.ca is out today: portlandpress-com.proxy3.library.mcgill.ca/biochemsoctr...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Latest from the lab! Analysis of everyone’s favorite regulatory mechanism in bacteria — the RF2 programmed frameshift! Likely present in the ancestor of bacteria, use of this mechanism is influenced by stop codon usage! Big congrats to @cassidyprints.bsky.social journals.asm.org/doi/10.1128/...

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

How can you find consistent biological 3D structures in heavily contaminated electron microscopy data sets without using any prior knowledge? #CryoEM #SingleParticle #Reconstruction doi.org/10.1107/S205...

Repost appreciated: Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics. Deadline 7 September. More info: www.carlsonlab.se/join/

Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions rdcu.be/ezlhg

wow, this is amazing!

Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...

A complex between IF2 and NusA suggests early coupling of transcription-translation… pubmed.ncbi.nlm.nih.gov/40715203/

Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out: www.sciencedirect.com/science/arti...

Finally out! I’m thrilled to share our new paper (Wolin et al., Cell 2025). This paper describes SPIDR, a high-throughput method for mapping RBP binding sites. By combining #SPIDR with #cryoEM, we identified the exact binding site of LARP1 within the #mRNA channel of the 40S ribosomal subunit.

New preprint alert! 🚨👇 I'm delighted to share the latest research from @jemmabetts.bsky.social in our group: ‘A new protein-dependent riboswitch activates ribosomal frameshifting’. Huge congratulations to Jemma for the first manuscript of her PhD! ✨ www.biorxiv.org/content/10.1...

Out now: Structure of an archaeal ribosome reveals a divergent active site and hibernation factor @jhdcate.bsky.social @banfieldlab.bsky.social @diptinayak.bsky.social & co #microsky www.nature.com/articles/s41...

Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...

Highly collaborative efforts that involved 6 lab members and the labs of @yiliangding.bsky.social and @rdaslab.bsky.social. We uncovered a telomerase dimer and its potential role in telomere maintenance.

🚨 New review alert! Our article "SEL1L–HRD1-mediated ERAD in mammals" is now online at Nature Cell Biology. We explore how this central ERAD pathway maintains protein homeostasis in mammals and why it matters for health and disease. 👉 rdcu.be/etk3b #ERAD #NatureCellBiology #SEL1L #HRD1 #ER

Excited to share this preprint from my lab. We report a potent and site-specific inhibition of translation initiation by 2’O methylation. It’s been a though year, but these discoveries keep us excited and motivated! #RNAsky #RNAbiology

Check out our latest preprint with the Qi lab on the structure and disease physiology of the human ERAD complex. Outstanding work by Leo, a cell physiologist whom we successfully tempted into structural biology! Preprint: www.biorxiv.org/content/10.1...

Happy that Jon Chen @jonchenjk.bsky.social's cryo-ET and confocal study of RPE-1 chromatin in situ is online www.embopress.org/doi/full/10.1038/s44318-025-00407-2 Congrats also to coauthors Tingsheng Liu, @shujuncai.bsky.social, Cai Tong Ng, Jian Shi and collaborators Weimei Ruan Uttam Surana.

We are excited to share our collab with @kedrov-lab.bsky.social! Using Cryo-EM❄️ on an amazing sample, we found that YidC is recruited to the back of SecYEG during late-stage substrate insertion —supporting that the "back-of-Sec" route observed in eukaryotes also exists in bacteria 🦠. Check it out👇👇👇

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social! www.biorxiv.org/content/10.1... #Chromatin #Asgard #Archaea #cryoEM

We are happy to share that our snR30 story is finally out in @natureportfolio.nature.com 🥳 We report the first structure of a H/ACA snoRNP acting in ribosome synthesis thereby providing a detailed structural and biochemical view of the snR30 snoRNP guiding local 18S rRNA subdomain folding. 👇👇👇

Delighted to share our newest publication - beautiful collaboration with Ya-Ming Hou’s lab. If you wonder whether bacterial mRNAs contain m6A (yes they do) and whether it matters (does not seem to) look here 👇🏻 academic.oup.com/nar/article/...

Early for #MicroscopyMonday: #Hemifusomes- Newly discovered ESCRT-independent intermediates of intraluminal vesicle and multivesicular body formation! In collaboration with the Kachar Lab at the NIH and out today in @natcomms.nature.com : rdcu.be/emtQu #cryoET #trafficking #organelle #hemifusion

Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay

mTORC1 is activated when cells are fed with amino acids. New preprint with @robzonculab.bsky.social led by Rachel Jansen shows how the massive "CastiGATOR" complex is formed when arginine-free CASTOR1 binds to the GATOR2 complex to antagonize mTORC1 starved cells. tinyurl.com/yr6en6f4

RIOK3 mediates the degradation of 40S ribosomes: Molecular Cell www.cell.com/molecular-ce...

>20 years ago, Zuker et al. discovered the mammalian sweet taste receptors Now, an exciting collaboration between our labs reveals the #cryoEM structure of the human sweet receptor-key to sweet taste perception-online @cp-cell.bsky.social! www.sciencedirect.com/science/article/pii/S0092867425004568

In our newly published study we show that the proline-rich antimicrobial peptide (PrAMP) Bac5 has a distinct mode of interaction with the ribosome compared to other PrAMPs…https://academic.oup.com/nar/article/53/8/gkaf324/8126264

Ribosome collisions and stalling senses m6A presence in mRNA which then starts degradation of m6A-containing mRNA. www.cell.com/cell/abstrac...

New paper from our lab! 🥳🎉 "UFMylation orchestrates spatiotemporal coordination of RQC at the ER" @Science Advances www.science.org/doi/10.1126/...

Modulation of protein activity by small RNA base pairing internal to coding sequences: Molecular Cell www.cell.com/molecular-ce...

The timeline of co-transcriptional #mRNA processing and #modification may have to be revised: cleavage, termination, and #m6A deposition may occur earlier than most #splicing! Potential textbook changing study in bioRxiv by edueyras.bsky.social & Rippei Hayashi labs! www.biorxiv.org/content/10.1...

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Cup? Platter? Stained glass? Our imaginations were inspired (even during uncertain times!) by these beautiful molecular complexes: the Erlin and prohibitin complexes implicated in quality control at the ER and mitochondria, respectively. Check them out here: www.biorxiv.org/content/10.1...

The cover of the April issue of @cp-trendspharma.bsky.social. Gregory Howard and William Tansey review the current status, challenges, and future prospects of cancer treatments that target the ribosome. Aberrant ribosome production and function are hallmarks of cancer.

Krista Freeman et al 𝘊𝘌𝘓𝘓 stunning atomic-level imaging (Cryo-EM, cryo-ET) reveals how 𝐛𝐚𝐜𝐭𝐞𝐫𝐢𝐨𝐩𝐡𝐚𝐠𝐞 Bxb1 reshapes its tail tip to breach the mycobacterial cell wall and deliver DNA to the cytoplasm can inform precise targeted phage therapies for TB and NTM infections www.cell.com/cell/fulltex...

(1/3) Together with the labs of Valerie Doye @vdoye.bsky.social and Hans-Georg-Kräusslich, we analyzed NPC structure in Nup133 null mESCs. We found heterogenous NPCs featuring non-canonical symmetries and missing subunits. Now online @naturecellbiology.bsky.social. www.nature.com/articles/s41...

Cool work from the Feaga lab 👇