Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com

See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026

We are advertising a PhD project investigating a cellular mechanism that ensures protein quality control during mRNA translation in plants. Please get in touch on here or via email if you are interested and I can provide further details! Lab website: sites.google.com/site/danielg...

Uptake of anti-cancer drugs depends on N-terminally acetylated anion channels | @ispt-proteinterm.bsky.social www.nature.com/articles/s42...

📢 Our October issue is now online. Access the full issue 👉 www.cell.com/trends/bioch.... 🎨 The cover highlights a Review from @chiosislabmskcc.bsky.social‬ and co on how PTMs reprogram chaperones into epichaperomes.

And now for something completely different in co-translational targeting: Molecular Cell www.cell.com/molecular-ce...

A Cell-Potent Bisubstrate Inhibitor to Probe NatD Acetyltransferase Activity | The inhibitor effectively reduced cellular Nα-acetylation on histone H4, leading to reduced migration in lung cancer cells | @acs.org ACS Chemical Biology @ispt-proteinterm.bsky.social pubs.acs.org/doi/10.1021/...

Protein N-terminal acetylation regulates the growth of rice plants | #NAA30 #NatC #rice #acetylation www.sciencedirect.com/science/arti...

Two new volumes of Methods in Enzymology with 29 chapters/papers on Protein termini methods and reviews are now out! Thx to all authors!🙌 @ispt-proteinterm.bsky.social #N-degron #prenylation #myristoylation #formylation #arginylation #oxidation #acetylation www.sciencedirect.com/bookseries/m...

Are you excited?? Because I am! 25 years ago I was 10 and had just started secondary school yet our journal was already covering groundbreaking science, long before I'd even heard of molecular cell biology 🧐 Safe to say I'm fairly familiar with it now 🤭 Enjoy our issue!!!!!!!!!!!

Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

Anatomy and habitat shape the oxygen sensing machinery of flowering plants | new preprint from Licausi lab @syno2xis.bsky.social #hypoxia #N-degron @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

New review on the generation and consequences of N-terminal proteoform diversity from Evan Morrison and Olivia Rissland | #N-degron #RNA @cp-cellreports.bsky.social www.sciencedirect.com/science/arti...

🎉 As we prep for #TIBSCelebrates50, I'd love for participation from YOU! Share your creativity and have your artwork featured on the cover of @cp-trendsbiochem.bsky.social! 📆 Deadline is Sept 30!!

Targeted degradation of pathogenic TDP-43 proteins in amyotrophic lateral sclerosis using the AUTOTAC platform | Yong Tae Kwon lab | #N-degron www.biorxiv.org/content/10.1...

A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | Preprint from @mikellavi.bsky.social @beagiuntoli.bsky.social @syno2xis.bsky.social #N-degron #hypoxia www.biorxiv.org/content/10.1...

Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...

Very cool stuff on the co-translational folding of dimers by Sander Tans and his team @amolf-nl.bsky.social: www.nature.com/articles/s41...

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin nature.com/articles/s41... #plantscience

Emmanuel Levy @elevylab.bsky.social has joined BlueSky 🌟 with a fantastic Cell paper with Shu-ou Shan, showing the interactome of the TOM complex and how cotranslational mito import prioritizes large globular domains. Beautiful science! Give him a warm welcome 🎉 link: www.cell.com/cell/fulltex...

Nitric oxide promotes cysteine N-degron proteolysis through control of oxygen availability | Important finding by Kim, Tian, Ratcliffe and Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron www.pnas.org/doi/10.1073/...

Disease resistance in rice steered by a long non-coding RNA via protein N-terminal acetylation | @ispt-proteinterm.bsky.social advanced.onlinelibrary.wiley.com/doi/10.1002/...

Melon wrinkles linked to protein N-terminal acetylation. link.springer.com/article/10.1...

Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...

Repost appreciated: Open #PhDposition in structural virology in my group! Join us in Umeå, Sweden to uncover how arboviruses remodel infected cells. In situ #cryoET and #cryoEM combined with #virology, #cellbiology, and #biophysics. Deadline 7 September. More info: www.carlsonlab.se/join/

💡Interested in our recently advertised @embl.org-style group leader positions but still not sure about it? Maybe this clip will convince you to join us👇!

Our 'Spotlight' discussing an excellent recent paper by @lauradc.bsky.social @n-end-rules.bsky.social & @syno2xis.bsky.social et al on the evolution of oxygen signalling in land plants is now online at Molecular Plant. Spotlight: tinyurl.com/7ydn6p4a Original paper: tinyurl.com/yw5yd4f8

📢 Our August issue is online! 🎨 The cover highlights a Feature Review from Stephanie Rosswag de Souza, @gzaffagnini.bsky.social, and @bokelab.bsky.social on how proteostasis functions in cellular dormancy across a number of examples, from yeast to adult quiescent stem cells and vertebrate oocytes.

Zebrafish model shows that NAA15 and N-terminal acetylation defects cause heart disease | Independently confirms the causal link between pathogenic NAA15 variants and congenital heart disease | Burns lab @bostonchildrens.bsky.social www.biorxiv.org/content/10.1...

Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...

How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Using a specific antibody to detect proteins carrying N-terminal formylation | Methods in Enzymology | Hwang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...

www.biorxiv.org/content/10.1... I am excited to share a big part of my thesis in preprint form, where we analysed the determinants of NAC-binding to translating ribosomes using selective ribosome profiling in C. elegans.

Making sense of oxygen sensing - Thomas Keeley & Peter Ratcliffe describe challenges encountered in measuring and manipulating cell monolayer oxygenation in vitro, and innovative approaches to overcome them www.embopress.org/doi/full/10....

Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Method to determine the identity and modification state of plastid precursor protein N-termini in the cytosol by combining protoplast protein import assays with targeted mass spectrometry | Methods in Enzymology | Baginsky lab. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

Engineered mutations in the AtPCO4 active site reduce its activity and improve plant submergence resilience! Our new preprint: tinyurl.com/2ydj7qx5, 1 of 2 preprints out this week from the @emilyflashman.bsky.social and @syno2xis.bsky.social labs to link PCO function in plants with flood tolerance!

Gid11 is a substrate receptor of the GID multisubunit E3 ligase during a metabolic switch. Specific degradation of proteins linked to glycolysis via their N-terminal threonine lacking N-terminal acetylation. #N-degron Khmelinskii lab @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...

Engineering reduced activity in the oxygen-sensing Arabidopsis thaliana plant cysteine oxidase 4 enzyme results in improved flood resilience. By Dirr et al. Licausi and Flashman labs @syno2xis.bsky.social @emilyflashman.bsky.social www.biorxiv.org/content/10.1...

Manipulating plant oxygen sensing through NCO substitution reveals trade-offs between growth and flooding tolerance. Perri et al. - another preprint from the Licausi and Flashman labs. @monicaperri1.bsky.social @emilyflashman.bsky.social @syno2xis.bsky.social www.biorxiv.org/content/10.1...

Excited to announce our new methods paper in Methods in Enzymology! We share our pipeline for building high-diversity DNA libraries & exploring protein stability in E. coli. Free access until Sept 4: authors.elsevier.com/a/1lRkeHRzCX... Updated, companion preprint: www.biorxiv.org/content/10.1...

New paper out in Mol Cell, where we show how N-myristolytransferases lipidate proteins on the translating ribosome! Continuing our successful collaboration with DeuerlingLab @uni-konstanz.de & Shu-ou Chan @caltech.edu @snsf.ch @ethz.ch www.cell.com/molecular-ce...

DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to use bacteria for arginylation assessment. Co-expression of ATE1 and substrate outlined by Yi Zhang and colleagues in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to detect protein N-terminal arginylation - a key step of protein degradation via the Arg/N-degron pathway. By Jasper Eising and @ndissmeyer.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

How to assess protein stability and N-degrons via the Ubiquitin reference technique. From the Protein Metabolism Lab (Yong Tae Kwon) at SNU Seoul ups.snu.ac.kr in Methods in Enzymology @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

📣 Our July issue is live! Inside, you'll find a variety of articles focused on #mitochondrialbiochemistry, plus an interview with many of the authors! The cover (by @brischigliaro.bsky.social) emphasizes one of these reviews from Antoni Barrientos et al. Find it here 👉 www.cell.com/biochemical-...