Cell Biology J-Club
@cellclub.bsky.social
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đ 1117
www.biorxiv.org/content/10.6...
Cholesterol-dependent lysosomal docking of mTORC1 mediated by TM6SF1
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https://www.biorxiv.org/content/10.64898/2026.04.01.715928v1
about 2 hours ago
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www.biorxiv.org/content/10.6...
The role of N-glycans and their processing in ER-to-lysosome-associated degradation of disease-causing mutant Neuroserpin
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https://www.biorxiv.org/content/10.64898/2026.04.02.716018v1
about 2 hours ago
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www.nature.com/articles/s41...
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Mechanisms of human mitochondrial leaderless mRNA translation initiation - Nature Communications
How human mitochondrial ribosomes initiate translation remains elusive. Here, the authors combined biochemical reconstitution, single-molecule fluorescence spectroscopy, and cryo-EM approaches to defi...
https://www.nature.com/articles/s41467-026-71535-4
about 2 hours ago
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www.biorxiv.org/content/10.6...
Genetic dissection of rapid proteolysis identifies TXNDC15 as a key factor of ERAD and lipid homeostasis
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https://www.biorxiv.org/content/10.64898/2026.04.01.715723v1
1 day ago
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Pearling drives mitochondrial DNA nucleoid distribution | Science
www.science.org/doi/10.1126/...
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Pearling drives mitochondrial DNA nucleoid distribution
The distribution of mitochondrial DNAâcontaining nucleoids is essential for mitochondrial function and genome inheritance; however, no known mechanisms can explain nucleoid segregation or their regula...
https://www.science.org/doi/10.1126/science.adu5646
2 days ago
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16
7
onlinelibrary.wiley.com/doi/10.1111/...
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KIF5B and DyneinâDependent Golgi Organization: Role in AdhesionâDependent Microtubule Acetylation
KIF5B and dynein regulate Golgi organization and localization- Role in regulating MT acetylation: In control (CNT) cells, active Arf1 facilitates the recruitment of KIF5B and dynein to the Golgi, mai...
https://onlinelibrary.wiley.com/doi/10.1111/tra.70033
3 days ago
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www.biorxiv.org/content/10.6...
ER discontinuities are common in C. elegans neurons, revealing a genetically tractable model for ER network maintenance
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https://www.biorxiv.org/content/10.64898/2026.03.31.715740v1
3 days ago
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www.biorxiv.org/content/10.6...
Selective MOSPD2-STARD3 interaction at ER contact sites governs late endosome/lysosome dynamics and cholesterol homeostasis
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https://www.biorxiv.org/content/10.64898/2026.03.30.714413v1
4 days ago
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www.nature.com/articles/s41...
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Programmable macromolecule delivery via engineered trogocytosis - Nature Cell Biology
Chen et al. engineer TRANSFER (trogocytosis-inspired receptor transfer and functional effector release), a method to deliver functional cargos from donor to recipient cells in a specific manner via di...
https://www.nature.com/articles/s41556-026-01920-0
4 days ago
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www.nature.com/articles/s41...
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TFEB degradation is regulated by an IKK/ÎČ-TrCP2 phosphorylation-ubiquitination cascade - Nature Communications
Inflammatory signaling profoundly influences cellular metabolism and stress responses. Here, the authors show that the IKK complex directly controls TFEB stability through ÎČ-TrCP2âdependent proteasoma...
https://www.nature.com/articles/s41467-026-71001-1
4 days ago
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STING signaling modulation by COPII cargo recognition
www.sciencedirect.com/science/arti...
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STING signaling modulation by COPII cargo recognition
Stimulator of interferon genes (STING) activation requires coat protein complex II (COPII)-mediated endoplasmic reticulum (ER) exit, but the mechanismâŠ
https://www.sciencedirect.com/science/article/pii/S0092867426002679
4 days ago
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www.biorxiv.org/content/10.6...
In situ visualization of autophagy suggests vesicle fusion can contribute to phagophore expansion
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https://www.biorxiv.org/content/10.64898/2026.03.29.715079v1
5 days ago
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www.nature.com/articles/s41...
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A pathogenic Tau mutation drives autophagy-lysosome dysfunction that limits Tau degradation in a model of frontotemporal dementia - Nature Communications
Researchers show that a disease-causing Tau mutation disrupts the autophagy-lysosome pathway in human neurons, leading to Tau accumulation, and that enhancing autophagy can partially restore protein c...
https://www.nature.com/articles/s41467-026-70473-5
5 days ago
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www.nature.com/articles/s41...
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Pathogenic KIF1A R350 mutations disrupt a conserved and conformation-dependent kinesin-tubulin salt bridge - Nature Communications
Distinct KIF1A mutations cause neurological disease, but the mechanism for many variants remains unclear. Here, the authors use cryo-EM and motility assays to show that R350 mutations disrupt a conser...
https://www.nature.com/articles/s41467-026-71026-6
5 days ago
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SPEx: Compartment-Resolved Proteomics via Expansion MicroscopyâGuided Microdissection
www.biorxiv.org/content/10.6...
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https://www.biorxiv.org/content/10.64898/2026.03.28.714993v1
5 days ago
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Compartmentalized cytoplasmic tradewinds direct soluble proteins
www.nature.com/articles/s41...
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Compartmentalized cytoplasmic tradewinds direct soluble proteins - Nature Communications
Cells use fluid flow to deliver proteins to their leading edge. An actin barrier creates a compartment where contraction drives flow, steering proteins toward growing regions. This mechanism coordinat...
https://www.nature.com/articles/s41467-026-70688-6
5 days ago
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Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formation
www.nature.com/articles/s41...
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Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formation - Nature Communications
Du, Miao et al identify that the spatiotemporal recruitment and activation of Rab5 during early endosome formation are collectively determined by Rabex5/hRME6 recruitment and PI(4,5)P2 depletion durin...
https://www.nature.com/articles/s41467-026-70543-8
5 days ago
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Revised: Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an endocytic initiator
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https://www.biorxiv.org/content/10.1101/2025.09.09.674816v2
6 days ago
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Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an endocytic initiator
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https://www.biorxiv.org/content/10.1101/2024.10.08.617284v2
6 days ago
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www.nature.com/articles/s41...
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Expanding the targeted protein degradation approach with small molecule chimeras directed to the 26S proteasome - Nature Communications
Targeted Protein Degradation (TPD) relies on the binding of specific targets and E3 ubiquitin-ligases to promote ubiquitination and degradation of targets by the proteasome. Here, by designing chimera...
https://www.nature.com/articles/s41467-026-71132-5
7 days ago
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Small-molecule CBLB inhibitor abolishes EGFR ubiquitination, reduces receptor endocytosis, and diminishes cell motility signaling | PNAS
www.pnas.org/doi/10.1073/...
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Small-molecule CBLB inhibitor abolishes EGFR ubiquitination, reduces receptor endocytosis, and diminishes cell motility signaling | PNAS
Endocytosis of the epidermal growth factor receptor (EGFR) is considered a key regulator of the receptor signaling activity. However, the molecular...
https://www.pnas.org/doi/10.1073/pnas.2524664123
8 days ago
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A general role for GGA adaptors in the modulation of AP-1-dependent trafficking
www.biorxiv.org/content/10.6...
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https://www.biorxiv.org/content/10.64898/2026.03.25.714221v1
8 days ago
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Regulation of microtubule abundance and minus end dynamics by Katanin, CAMSAPs, WDR47 and kinesin-13
www.biorxiv.org/content/10.6...
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https://www.biorxiv.org/content/10.64898/2026.03.26.714132v1
8 days ago
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VAMP8 function reveals tight linkage between endocytic recycling and endocytosis | PNAS
www.pnas.org/doi/10.1073/...
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PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
https://www.pnas.org/doi/10.1073/pnas.2602991123
8 days ago
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A molecular switch in NAC prevents mitochondrial protein mistargeting by SRP
www.nature.com/articles/s41...
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A molecular switch in NAC prevents mitochondrial protein mistargeting by SRP - Nature Communications
How ribosome-bound NAC distinguishes mitochondrial precursors from ER clients has remained unclear. Here, authors reveal a molecular switch in NAC that limits SRP access to nascent mitochondrial precu...
https://www.nature.com/articles/s41467-026-71061-3
8 days ago
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Proximity labelling of the BAK macropore uncovers a new role for SLC35A4-MP in mitochondrial dynamics
www.biorxiv.org/content/10.6...
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https://www.biorxiv.org/content/10.64898/2026.03.22.713508v1
11 days ago
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link.springer.com/article/10.1...
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The coordinated action of UFMylation and the RQC pathways clears arrested polypeptides at the ER - The EMBO Journal
Clearance of arrested nascent polypeptides resulting from ribosomal stalling is essential for proteostasis. Stalled endoplasmic reticulum (ER)-bound ribosomes are marked by ubiquitin-fold modifier 1 (...
https://link.springer.com/article/10.1038/s44318-026-00753-9
11 days ago
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Human dyneinâdynactin is a fast processive motor in living cells
doi.org/10.7554/eLif...
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Human dyneinâdynactin is a fast processive motor in living cells
TIRF imaging of CRISPR-edited human cells shows dyneinâdynactin complexes move processively with motility parameters consistent with the presence of multi-dynein assemblies (>one dynein heavy chain dimer per dynactin) in cells.
https://doi.org/10.7554/eLife.94963.3
11 days ago
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www.nature.com/articles/s41...
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Atlas of predicted protein complex structures across kingdoms - Nature Communications
Protein complexes are the machinery of life, yet mapping their structures across different species is challenging. This study presents an atlas of 1.1million cross-kingdom structures, revealing 181,67...
https://www.nature.com/articles/s41467-026-70884-4
11 days ago
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Pan-neurodegeneration proteomics reveals disease subtypes and molecular signatures: Cell
www.cell.com/cell/fulltex...
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Pan-neurodegeneration proteomics reveals disease subtypes and molecular signatures
A pan-neurodegeneration atlas built from multilayer, deep proteomics of 2,279 brain samples across 6 major diseases integrates whole proteome, detergent-insoluble proteome, and posttranslational modif...
https://www.cell.com/cell/fulltext/S0092-8674%2826%2900233-3
11 days ago
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In
@eLife.bsky.social
: Engineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular Lifespan
doi.org/10.7554/eLif...
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https://doi.org/10.7554/eLife.109761.1
11 days ago
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Structural transitions in the stepwise assembly of proteasome core particles
www.nature.com/articles/s41...
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Structural transitions in the stepwise assembly of proteasome core particles - Nature Communications
Eukaryotic 20S proteasomes assemble from 14 distinct subunits. Here, the authors use cryo-EM to analyze multiple intermediates showing that biogenesis involves alternative routes, rather than one fixe...
https://www.nature.com/articles/s41467-026-70525-w
11 days ago
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Myofibers drive postnatal myonuclear accretion through Arp2/3-dependent membrane protrusions
www.biorxiv.org/content/10.6...
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https://www.biorxiv.org/content/10.64898/2026.03.22.713469v1
12 days ago
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link.springer.com/article/10.1...
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CaÂČâș leakage is a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repair - The EMBO Journal
Endomembrane damage of intracellular vesicles triggers signals that activate membrane repair in mammalian cells to restore homeostasis. However, the signals that drive diverse membrane repair recruitm...
https://link.springer.com/article/10.1038/s44318-026-00741-z
15 days ago
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www.nature.com/articles/s41...
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Visualizing suborganellar lipid distribution using correlative light and electron microscopy - Nature Cell Biology
Lennartz et al. introduce a correlative light and electron microscopy workflow, Lipid-CLEM, combining near-native lipid probes and on-section labelling via click chemistry. Lipid-CLEM quantitatively a...
https://www.nature.com/articles/s41556-026-01915-x
15 days ago
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Spatial and morphological organization of mitochondria in neurons across a connectome | Science
www.science.org/doi/10.1126/...
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Spatial and morphological organization of mitochondria in neurons across a connectome
Neuronal function depends on mitochondria, but little is known about their organization across neurons. Using an electron microscopy Drosophila connectome, we uncovered quantitative rules governing th...
https://www.science.org/doi/10.1126/science.ads6674
16 days ago
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SEL1LâHRD1 ERADâautophagy interplay maintains mitochondrial homeostasis in brown adipocytes | PNAS
www.pnas.org/doi/10.1073/...
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SEL1LâHRD1 ERADâautophagy interplay maintains mitochondrial homeostasis in brown adipocytes | PNAS
Mitochondrial integrity is central to energy homeostasis, particularly in brown adipose tissue where dynamic remodeling fuels thermogenesis. Two ma...
https://www.pnas.org/doi/10.1073/pnas.2529914123
16 days ago
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A PTPN23-dependent ESCRT pathway is essential for constitutive secretion in mammalian cells url:
rupress.org/jcb/article-...
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A PTPN23-dependent ESCRT pathway is essential for constitutive secretion in mammalian cells
Stalder and Pereira et al. combine affinity isolation of post-Golgi carriers, mass spectrometry, and a pooled CRISPR-KO screen to uncover new regulators of
https://rupress.org/jcb/article-abstract/225/5/e202506111/281617/A-PTPN23-dependent-ESCRT-pathway-is-essential-for
16 days ago
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Mitochondrial control of glycerolipid synthesis by a PEP shuttle: Cell
www.cell.com/cell/fulltex...
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Mitochondrial control of glycerolipid synthesis by a PEP shuttle
SLC25A35 is revealed as the mitochondrial PEP exporter that licenses glycerolipid synthesis in lipogenic cells, linking mitochondrial energetics to lipid production. Mitochondrial PEP flux drives glyc...
https://www.cell.com/cell/fulltext/S0092-8674%2826%2900224-2
18 days ago
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www.biorxiv.org/content/10.6...
Lipid droplets accumulate and delay regulated cell death execution
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https://www.biorxiv.org/content/10.64898/2026.03.16.712084v1
18 days ago
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www.biorxiv.org/content/10.6...
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Dynein-dependent positioning of multiple organelles regulates adaptive gene expression during oxidative stress
Cells respond to environmental insults through well-characterized changes to their transcriptome and proteome. The contribution that reorganization of intracellular architecture makes to the adaptive stress response is much less understood. Here, we reveal a conserved spatial response to oxidative stress in which the microtubule motor dynein enhances perinuclear clustering of multiple membrane-bound organelles. These events are triggered by reactive oxygen species and protein kinase C and occur without increased association of dynein with its activator dynactin, pointing to a non-canonical mechanism for stimulating retrograde transport. Using a chemically-inducible system for dispersing individual organelles, we show that perinuclear localization of multiple compartments potentiates oxidative stress-induced gene expression, with distinct genes depending on inputs from either combinations of organelles or individual compartments. Together, these findings implicate positioning of multiple membrane-bound compartments in gene expression regulation and identify coordinated organelle trafficking by dynein as an additional regulatory layer in the cellular stress response. ### Competing Interest Statement The authors have declared no competing interest. Medical Research Council, https://ror.org/03x94j517, MC_U105178790, MC\_UP\_1201/24 Engineering and Physical Sciences Research Council, https://ror.org/0439y7842, EP/X023044/1 LMB-AstraZeneca Blue Sky Fund, BSF 2-23 EMBO Young Investigator
https://www.biorxiv.org/content/10.64898/2026.03.17.712024v1
18 days ago
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www.nature.com/articles/s41...
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Paraspeckle condensation is controlled via TDP-43 polymerization and linked to neuroprotection - Nature Cell Biology
Hodgson, Huang, Lang et al. show that TDP-43 limits ribonucleoprotein particle condensation into paraspeckles in a concentration- and polymerization-dependent manner. They also link paraspeckle conden...
https://www.nature.com/articles/s41556-026-01895-y
18 days ago
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www.biorxiv.org/content/10.6...
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Actin-membrane interface stress regulates Arp2/3-branched actin density during lamellipodial protrusion
Motile cells can sense and exert forces on the extracellular environment through dynamic actin networks. Increased stress against the polymerizing barbed ends of branched actin networks has been shown to lead to an increase in the density of these networks through a force feedback mechanism, though this phenomenon has not been explored through the examination of real-time responses of endogenous actin networks in cells. Here, we utilize mouse embryonic fibroblast CRISPR knock-in lines with labeled ARP2/3 complex to identify cellular and extracellular conditions that regulate branched actin density and enrichment at the leading edge of lamellipodial protrusions. A common theme shared among all branched actin density-increasing conditions is higher levels of interface stress between the plasma membrane and the barbed ends of the lamellipodial actin network. Among these conditions, we find that ARP2/3 is specifically required for robust spreading and protrusion in response to increased extracellular viscosity. Interestingly, time-lapse traction force microscopy of ARP2/3-dependent viscosity responses show significantly reduced changes in strain energy applied to the substrate when compared to spreading and motility through cell-matrix adhesion. In addition, we find that increased extracellular viscosity can bypass the need for extracellular matrix proteins to support lamellipodial protrusion driven by optogenetic Rac activation. Our studies provide strong support for in vitro models of branched actin force feedback responses and further characterize an essential role for branched actin in mediating dramatic cell shape changes in response to increased extracellular viscosity. ### Competing Interest Statement The authors have declared no competing interest. NIGMS, R35GM130312, R35GM158040, F32GM131578
https://www.biorxiv.org/content/10.64898/2026.03.06.710140v2
19 days ago
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Condensate-mediated shape transformations of cellular membranes by capillary forces | PNAS
www.pnas.org/doi/10.1073/...
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PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
https://www.pnas.org/doi/10.1073/pnas.2424126123
19 days ago
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The α-synuclein proteostasis network and its translational applications in Parkinsonâs disease | PNAS
www.pnas.org/doi/10.1073/...
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PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
https://www.pnas.org/doi/10.1073/pnas.2513317123
19 days ago
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www.nature.com/articles/s41...
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Calcium-mediated calreticulin-IRE1α interaction drives dynamic fluctuation of IRE1α activity under chronic endoplasmic reticulum stress - Nature Communications
Ca2+ concentration-mediated, seesawed interactions between IRE1α and two chaperones, CALR and BIP, were discovered to drive IRE1α activity fluctuation that was observed under chronic endoplasmic retic...
https://www.nature.com/articles/s41467-026-70679-7
19 days ago
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www.nature.com/articles/s41...
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Structures of respiratory supercomplexes and ATP synthase oligomers in mammalian mitochondrial inner membrane - Nature Communications
Structures of mitochondrial ATP synthase oligomers and respiratory supercomplexes from sub-organellar particles (isolated from bovine heart mitochondria) offer insights into mitochondrial architecture...
https://www.nature.com/articles/s41467-026-70578-x
19 days ago
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Desmosomes compartmentalize mRNA and translation in the skin: Developmental Cell
www.cell.com/developmenta...
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Desmosomes compartmentalize mRNA and translation in the skin
DâAlessandro et al. reveal that skin cells compartmentalize translation at their cortex in a desmosome-dependent manner. Wounds induce translational changes linking cell adhesion to adaptive translati...
https://www.cell.com/developmental-cell/fulltext/S1534-5807%2826%2900078-X
19 days ago
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www.nature.com/articles/s41...
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Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesion - Nature Cell Biology
Hoffmann et al. show that the glycolytic intermediate fructose-1,6-bisphosphate, which accumulates in the absence of aldolase, regulates focal adhesions by relieving RCC2-mediated Rac1 inhibition.
https://www.nature.com/articles/s41556-026-01911-1
19 days ago
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www.nature.com/articles/s41...
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Magnaporthe oryzae MoPh1 perceives ER stress and promotes adaptive responses via a plasma membrane-to-vacuole pathway - Nature Communications
The unfolded protein response is activated at the ER due to protein misfolding. Here the authors show that in Magnaporthe oryzae, the plasma membrane localized MoPh1 protein perceives stress through E...
https://www.nature.com/articles/s41467-026-70610-0
19 days ago
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