Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...

I love AlphaFold—but please include PAE (Predicted Aligned Error) plots for every “interaction.” Pretty PDBs ≠ proof. If the PAE doesn’t show an interface, it ain’t one. Show the plots. Structural biology's reputation is on the line.

Thrilled our study on heme crystal motion in #malaria @parasitesrule.org is now published @pnas.org, support from NIH, @bwfund.bsky.social, @uofutahcihd.bsky.social, UofU 3i. With Fu and Bidone labs @price.utah.edu, @uofubiochem.bsky.social, @uofuhealth.bsky.social www.pnas.org/doi/10.1073/...

We have a new preprint for you. Naked mole rats are very long-lived rodents and have increased translational fidelity. Interestingly, 28S ribosomal RNA is split. Here we investigated the structure of the ribosome by single particle cryo-EM. Take a look. www.biorxiv.org/content/10.1...

Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️ A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria

It was so fun to work on this with @gkocker.bsky.social and @clienkaemper.bsky.social! It is out @pnas.org If you love olfaction and love math, this one is for you! 🦟👃🧮 When noncanonical olfaction is optimal | PNAS www.pnas.org/doi/10.1073/...

Excited to share that our paper on mitochondrial cardiolipin dynamics is published in @natcomms.nature.com today! We provide molecular explanations for unique aspects of mitochondrial morphology and the mechanisms underlying cardiolipin-related Barth Syndrome. www.nature.com/articles/s41...

Behind every scientist is an army of workers who keep the biomedical research ecosystem running. Hear from these workers and how they contribute at www.nycures.org/ and SIGN UP to have your voice heard!

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles. We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography zenodo.org/records/1717...

🚀 Check out our new preprint on extending the scope of single-particle cryo-EM with 2D template matching! Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching is now available on bioRxiv! www.biorxiv.org/content/10.1...

This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

🚀 NY-CURES Launch — Championing State-Wide Investment in Science! 🚀 Our new coalition advocates for sustained public support of New York’s vibrant biomedical research ecosystem — creating cures, economic gains & strengthening communities. Sign up. Stay informed. Join the movement: www.nycures.org/

Stopping Ribosomes in Action!: a publication highlight from @keandreyam.bsky.social featuring the lab of @cmholab.bsky.social of @columbiauniversity.bsky.social & colleagues. Read more here: buff.ly/jWYKq7r #SBGrid #CryoET

Want to know how lipidation of some nascent chains takes place by NMT2-- Check our latest work on how NAC couples Protein Synthesis with Nascent Polypeptide Myristoylation on the Ribosome out today @embojournal.org‬: www.embopress.org/doi/full/10....

Cryo-EM of fertilisation complex Pfs230-Pfs48/45 from malaria parasites! 💕 FREE ACCESS reprint link found here: findaresearcher.wehi.edu.au/tham 📱click on 🌎 and last link 💻 last link on the left hand side #malaria #transmission #cryoEM #ScienceResearch www.science.org/doi/10.1126/...

New online: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Right now, the continuation of many restored NIH grants relies on the SCOTUS shadow docket. SCOTUS is not immune to pressure. Also, if you are represented by one of the 14 R senators that called for the restoration of NIH grants, please write them a thank you. Good behavior should be rewarded.

Submit your method to our denoising challenge!!! 👍

Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social. www.science.org/doi/10.1126/...

DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...

In our latest preprint on bioRxiv, we present the in situ structure of the human gap junction at 14 Å resolution using cryo-ET. The structure provides a blueprint for investigating how connexin regulation shapes intercellular communication in health and disease.

An Evaluation of Biomolecular Energetics Learned by AlphaFold by Lyu, Herschlag et al doi.org/10.1101/2025... Interesting comparison of AF2/3 structures with the PDB (AF2 appears better) Reminder: The PDB itself is probably biased relative to solution conformational ensembles and energetics

Our work, co-led by @alfredo0712.bsky.social and @nlue8.bsky.social is out in @natsmb.nature.com Shout out to coauthors P.Grob, B. Kaeser, J.Fang, and S. Kassube for their contributions and thanks to the reviewers for the suggestions that led to the final work. www.nature.com/articles/s41...

In-situ SPA 🧨 www.biorxiv.org/content/10.1...

Machine learning enables de novo multi-epitope design of plasmodium falciparum circumsporozoite protein to target trimeric L9 antibody www.biorxiv.org/content/10.1101/2025.06.29.662177v1 #cryoem

Forward-looking preview by Bin Wu to our latest paper in Structure by @andrewnchang.bsky.social highlighting the power of #AI in #cryoEM of patient-derived samples: tinyurl.com/tu2fyvme Paper: tinyurl.com/3cycffzm Many thanks to @thorstenwagner.bsky.social @raunser-lab.bsky.social for #crYOLO

Vertebrate retrotransposons are the future of gene therapy. But how do they insert their genes? 🔥🔥 Thrilled to share our new work now published with Kathy Collins, @nogaleslab.bsky.social @berkeleymcb.bsky.social where we investigate this with #cryoEM & biochemistry in 🧪 and cells! #RNAsky #TEsky

Cargo adaptors use a handhold mechanism to engage with #myosin V for #organelle transport, say Hye Jee Hahn, Natalya Pashkova, Michael Cianfrocco, & Lois Weisman @umlifesciences.bsky.social rupress.org/jcb/article/... #Microbiology #Organelles #Trafficking

Exited to share my first PostDoc contribution! Like Magellan explored the globe, you can explore your cryoEM data with Magellon. Congrats to all involved. Scott Stagg Lab & Michael Cianfrocco Lab @landerlab.bsky.social www.biorxiv.org/content/10.1... 🌎❄️🔬

Ever wonder why SGD, which works so well for ab initio cryo-EM, never seems to get to high resolution on its own? Well, we have the answer: conditioning!

New preprint from Daniel Du @fitzpatricklab.bsky.social shows #femtosecond light pulses can lens electron beams, promising higher contrast in #cryoET and better aberration correction in #cryoEM tinyurl.com/257sw7j9 Experiments made possible by support from @cziscience.bsky.social #ImagingTheFuture

"Unveiling the structural spectrum of SARS-CoV-2 fusion by in situ cryo-ET" www.nature.com/articles/s41...

We have a preprint for you EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

Awesome malaria study out now in Nature Microbiology: An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission By Franziska Hentzschel, Matthias Marti, Freddy Frischknecht & colleagues! @freddyfrischknecht.bsky.social www.nature.com/articles/s41...

A dynamic barrier: remodeling of the nuclear envelope during closed mitosis in malaria parasites pubmed.ncbi.nlm.nih.gov/40488521/ #cryoem

Registration and Abstract submission for MPMXXXVI are now open! Abstract submission deadline is June 27th, get yours in quickly. @rmc9z.bsky.social @mjgubbels.bsky.social @omarharb.org 🔗https://www.parasitesrule.com/mpm-xxxvi

our lab is blessed to have 3 new postdoctoral fellow positions. we would welcome scientists interested in...

Cutler & Glaser in JAMA: The proposed budgetary cuts to NIH will create a social cost 16 times greater than the savings the administration is attempting to achieve. The Trump administration should recognize that funding science is good economic as well as health policy.

Meeting of the four #cryoET centers (funded by the NIH Common Fund), in beautiful Boulder! great exchange of ideas and totally nerding it out over all things cryoET. Great to hear from users from all across the country and their impressive science. #NIH at its best.

Huge congratulations to @hbelda.bsky.social and David Bradley for their publication on the extraordinarily interesting FIKK kinase family (I am biased) of the malaria causing parasite Plasmodium falciparum. 1/n

We are looking to hire (yes, even in this economy!) a jr. specialist to train in protein prep/structural biology related to our AVOID-ome work as part of openadmet.org. A great position for someone who is looking to be a tech for a few years before grad or med school. aprecruit.ucsf.edu/JPF05424

Parasites need mitochondrial respiration to infect and spread. Effective anti-malaria and ani-toxoplasmosis drugs target respiratory complexes (e.g. atovaquone)! But how do the parasite complexes work and how do drugs inhibit them? Read Andrew Maclean and Alex Mühleip phenomenal work on this! 👇

Structure, assembly and inhibition of the Toxoplasma gondii respiratory chain supercomplex pubmed.ncbi.nlm.nih.gov/40389671/ #cryoem

Please consider applying for a post doc in our lab if you have bioinformatics and wet lab skills, and are interested in parasites! elxw.fa.em3.oraclecloud.com/hcmUI/Candid...

Superb and important article, pointing out that politically the best way to save biomedical research is to get communities around different diseases informed of the problem, and set them loose on their congresspeople. Right now they just don't know what's going on. How to make this happen?

A Path Forward to Save American Bio-Medical Research talkingpointsmemo.com/edblog/a-pat...

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals. www.science.org/doi/10.1126/...

Nobel Prize winner @kkariko.bsky.social ✅ Olympic gold medalist Susan Francia ✅ Netflix star Dr. David Langer ✅ #ICYMI, the first episode of The Leap is available now. From @hypothesisfund.bsky.social, via @scifri.bsky.social: pod.link/73329284/epi...