1/16 New pre-print from the Sternberg Lab! We uncover how temperate phages can use RNA-guided transcription factors to remodel the flagellar composition of their bacterial host and enhance their fitness. Find the preprint and full story here: tinyurl.com/mshwjd77

Gene editing pioneer David Liu discusses baby K.J. Muldoon, the first person treated with a customized gene editing therapy, what made K.J.'s treatment possible, and how on-demand treatments for rare genetic diseases could one day become routine. #GeneEditing #Science

🧬🌽 Happy Transposon Day! 🌽🧬 Today we celebrate the birthday of Barbara McClintock - scientist extraordinaire and discoverer of jumping genes. Still the only woman to have an unshared Nobel Prize in the biomedical sciences #TransposonDay2025

Nature invented CRISPRa way before scientists did 🤩 Awesome work by the @sternberglab.bsky.social Looking forward to seeing cool applications coming out of this !

Did you think you needed a promoter for CRISPRa? Think again!

1/10 New pre-print(s) from the Sternberg Lab in collaboration with Leifu Chang's Lab! We uncover the unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs. Full story here: www.biorxiv.org/content/10.1...

Did you see the recent buzz about evoCAST, a new CRISPR-transposon tool that can insert entire genes into human cells? We've got a quick explainer on how CASTs work and how evoCAST improves on them! @isaacwitte.bsky.social @sternberglab.bsky.social blog.addgene.org/cas...

We're thrilled to share the published version of our DRT9 story, online today @nature.com! Congratulations to all authors! www.nature.com/articles/s41...

Always grateful to investigators like @sternberglab.bsky.social and David Liu who share their plasmids through @addgene.bsky.social. www.addgene.org/browse/artic...

"..one of the biggest challenges right now for evoCAST, and other large DNA editing tools under development, is delivery. 'How do we actually get these tools and their payloads into the cells or tissues of interest?'” www.cuimc.columbia.edu/news/sternbe...

www.nature.com/articles/d41... Coverage of our collaboration with the Liu group.. ' “This is crazy directed evolution!” - Makoto Saito'

Excited to introduce evoCAST: a platform for efficient, programmable gene integration in human cells. This work, a close collab between my PhD lab (Liu group) and @sternberglab.bsky.social, has been such a fun journey to help lead. Huge thanks to all contributors! www.science.org/doi/10.1126/...

1/10 Today in @science.org in collaboration with the Liu group we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells. drive.google.com/file/d/1I-Ub...

💥🥳 At long last, our latest paper is out! Gag proteins of endogenous retroviruses are required for zebrafish development www.pnas.org/doi/10.1073/... Led heroically by Sylvia Chang & @jonowells.bsky.social A study which has changed the way I think of #transposons! No less! 🧵 1/n

Join GEN on June 11 for: "The State of CRISPR & Genome Editing" virtual summit. Guests include: Feng Zhang, @urnov.bsky.social , @juliemakani.bsky.social @nevillesanjana.bsky.social; Alexis Komor & Nicole Gaudelli, and Beth Shapiro Registration is free! webinars.liebertpub.com/e/the-state-...

One of my students pointed me to this great podcast that gives an overview of phage history! #PhageSky open.spotify.com/episode/4XTa...

We've been fortunate to celebrate a few wins in the lab recently. Dr. George Lampe received the Columbia Dean’s Award and Henry Le was awarded a Soros Fellowship. Cheers and congratulations to you both!!

Hello BlueSky! Inaugural post here from the Sternberg Lab. We're excited to share our latest work, in which we teamed up with the @WiedenheftLab to study how DRT9 reverse transcriptases provide antiviral immunity. Here’s what we found: www.biorxiv.org/content/10.1...