I had a wonderful time in Japan 🗼! Huge thanks to @ken-wakaba.bsky.social for inviting me to Sangyo University in Kyoto. Thanks to Takashi Yamano (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting! Great science and discussions!

Mitochondria finally made the headlines, yay! Oh wait, ... oh no ....

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Very cool! How do cytosolic ribosomes discriminate between ER and mitochondria targeted proteins? NAC recognizes mitochondrial targeting sequences! Very important since over 95% of the mitochondrial proteome is translated in the cytosol and then imported to mitochondria. Congrats!

A step towards easier control over mitochondrial gene expression: Silencing mitochondrial gene expression in living cells | Science www.science.org/doi/10.1126/...

Cell-to-cell connections in plants 🌿! Using cryo-ET 🔬 to have a look at plasmodesmata. Congrats Marcel, Matthias and co! You managed to push it far beyond what I thought was possible !

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Cool paper from cool people 😎 Expand all the things!

Curious about dinoflagellates? Tiny water bugs with super cool cellular architecture? Check out this cool initiative from my colleague @manondemulder.bsky.social and her friends! Their 1st online seminar is next month, don't miss it!

Re-redeading this one 📖. Great book for evolution and bioenergetics aficionados. The view is a bit distracting though ⛵

Happy to present the lab latest work, today from the green-side. The ultimate PSII assembly intermediate that directly precedes incorporation of the Mn4O5Ca cluster and formation of the OEC. Psb32 binds to PsbV, challenging the assumption the extrinsic subunits all associate spontaneously in-vivo.

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Also want to highlight that the script is based on the awesome aretomo3torelion5 from @phaips.vd.st 🤩 github.com/Phaips/areto...

To all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging: github.com/Chromatin-St... #Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Check out the latest version of MemBrain, spearheaded by computation superstar @lorenzlamm.bsky.social ! It can segment, pick particles and give you metrics on everything!

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

From my colleague @cellarchlab.com and his lab at the @biozentrum.unibas.ch I continue to be amazed by what cyro-electron tomography can do...

Congratulations Florent and coauthors!!! I'm very excited to continue diving into diversity and beauty of mitochondrial respiratory complexes in algae 😍

Out just now @naturecomms.bsky.social: using cryo-EM and cryo-electron tomography, we provide evidence that structurally heterogeneous ribosomes can cooperate in protein synthesis in bacterial cells: www.nature.com/articles/s41...

Attention mitochondriacs! Mitochondrial supercomplexes by cryo-electron tomography. How can you not be blown away by how beautiful this is? Fascinating that ATP synthase is largely organized as dimers since this is one of the putative configurations of mPT pore. www.science.org/doi/10.1126/...

Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6

I'm super happy that our story is now published! 📖 www.science.org/doi/10.1126/... But what changed compared to the original preprint? Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.

🔬⚡🦠 #Biologists have observed the proteins that generate #cellular energy with unprecedented resolution directly within the cell. The SNSF-funded study was conducted by @florentwaltz.bsky.social and his colleagues from the @unibas.ch and featured on the front page of #Science. ➡️ buff.ly/aUR6uf6

🔬⚡🦠 Des #biologistes ont observé avec une résolution inédite et à même la #cellule les protéines produisant l’énergie cellulaire. L’étude, menée avec le soutien du FNS par @florentwaltz.bsky.social et ses collègues de @unibas.ch , a fait la Une de #Science. ➡️ buff.ly/n2CpEbv

We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨

Weeeeeeeee (lift out)

This is the kind of story I enjoy reading! Congrats to the authors 🦠!

Running some experiments in Lausanne. The view never disappoints

To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Congratulations Marcel, Matthias & co! Remarkable technical achievement. At the EMBL tomo meeting, we've seen vitreous mouse brain biopsy, but plants 🌱 are more challenging because they are full of water 💧

Our methods paper made it to bioRxiv: doi.org/10.1101/2025... Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5 #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM

💡 At a joint press conference, the #SNSF and its ERI partners called on the Federal Council to abandon the planned cuts to #education, #research and #innovation in the "relief package 27", as they would have far-reaching consequences for the #economy and society. ➡️ https://buff.ly/3CTR2AM

We (@cellarchlab.com peeps, @manondemulder.bsky.social @phaips.vd.st @fannyleblanc.bsky.social @computingcaitie.bsky.social and Ricardo) had a blast at the EMBO in situ meeting in Heidelberg! Meeting old friends and making new ones! Lots of cool science and new ideas to try 👨🏻‍🔬🔬

Dipping into the archives today for this hydroid, Orthopyxis integra, covered in diatioms from the genus Licmophora. I have to admit, it’s one of my favourite images. 🦑

Congrats Priya! Very cool cryo elemental NanoSIMS, and on plant tissue 🥦 !

Finally out! Working with this team was a fantastic learning experience. Also I was excited to determine the in situ clathrin structure for this project! That’s my first EM-structure upload to EMDB. Can I tell my mom I am a structural biologist now? 😁 #cryoET

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

Check this out, maybe your last read of the year, or the first for the next one 📖? We used Chlamydomonas 🦠 to push cellular tomography towards the next step and made all the data public! BTW it's the same dataset I used for the mitochondrial respirasomes www.biorxiv.org/content/10.1... Enjoy 🥳!

More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy! Check out the preprint: www.biorxiv.org/content/10.1... Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com #TeamTomo

Thanks @bioversechronicles.bsky.social for highlighting our work on diatom #CO2 fixation in this cool video! 🧪🧶🧬🌍🌾🌊 #TeamTomo @biozentrum.bsky.social

Flabbergasted 🤯 by this amino acid resolution level crosslinking mass spec analysis from my colleague @computingcaitie.bsky.social Go check it out! (cilia still for the sillies though 🤪)

Mirror life dialogue must be global and equitable. Honored to write op-ed w/ co-author, Wilmot James, former MP and Shadow Health Minister in South Africa, relating new findings to my work on AMR and COVID-19 and Dr James’ work towards global health equity. www.dailymaverick.co.za/article/2024...

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @jonmarkert.bsky.social! tinyurl.com/setd2