🚨 Preprint alert! We solved the structure of human NSUN2 bound to Asp tRNA, revealing how it installs m⁵C and recognizes RNAs #CryoEM #RNABiology A collab between Casañal & Gullerova labs, with @miclass.bsky.social and me working side by side as co-first authors 🔗 doi.org/10.64898/202... (0/7🧵)

You want to work on carbon concentration mechanisms in algae 🫑? Go work with my (soon-to-be-former) colleague @manondemulder.bsky.social in Vienna @gmivienna.bsky.social ! Cryo-ET 🔬 and funky bugs 🦠 #CryoET #CryoEM

Excited to announce I’m starting a new lab at @gmivienna.bsky.social (Vienna BioCentre) in Sept 2026 We study how carbon fixation is inherited and maintained in algae using cryo-ET, imaging, environmental sampling&more Looking for people who want to build the lab together, get in touch! #teamtomo

Our #preprint list is now up on FocalPlane. Start your week feeling inspired by the latest research using microscopy to answer questions in biology. Let us know if you have any recommendations for us to add. focalplane.biologists.com/2026/03/09/m...

Happy to share our new preprint on the mechanism of human tRNA 3' CCA maturation! This project was spearheaded by Bernhard Kuhle in my group, with contributions from many others and a great collaboration with the group of Peter Rehling (UMG). See highlights below! www.biorxiv.org/content/10.6...

There is a week left to apply for two available positions in my lab @ipbhalle.bsky.social - PhD: ipb-halle.mhm.jobs/12-phd-posit... - Postdoc: ipb-halle.mhm.jobs/13-postdocto... Earliest start date: 01/05/2026. We are interested in plant immune receptor biochemistry and evolution. Please share!

Had a great time in Zurich for the annual LS² meeting. Nice gathering of the swiss scientific community, always cool to see what the neighbors are doing 😜

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome https://www.biorxiv.org/content/10.64898/2026.02.07.704542v1

Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome www.biorxiv.org/content/10.64898/2026.02.07.704542v1 #cryoem

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

For #ECR’s: DBG’s Hot Tpoic Workshop on Cryo-Electron Tomography + FIB-milling 13 – 15 April 2026 In @cellarchlab.com 's lab @biozentrum.unibas.ch Organised by @tamb-o.bsky.social, Karen Zinzius, @fannyleblanc.bsky.social Details: www.deutsche-botanische-gesellschaft.de/en/about-us/...

Am I the only one increasingly annoyed by cryo-EM papers not showing *any* experimental density in figures? Sometimes not even in the supplementary material??

Check this new study on carbon concentration mechanisms! See how the pyrenoid changes by modifying Rubisco linkers in our favorite alga, Chlamy 💚! My desk mate @phaips.vd.st spent a lot of time collecting & carefully analyzing these beautiful tomograms 🔬. Congrats to all the authors!

You like #LLPS? #TeamTomo? #Rubisco? Check out our latest preprint! What a great collaboration this has been!

This one was quite the journey! The paper describing the #ChlamyDataset is finally out and on the cover of Mol Cell! This beautiful rendering made by co-author @jessheebner.bsky.social and Holly Peterson shows an instance of mitochondrial fission found in the dataset 😍 [Maybe long thread ahead]

The latest issue is now online www.cell.com/molecular-ce...

Online Now: Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii Online now:

Japan is joining Horizon Europe, the EU’s flagship research and innovation programme. Openness and international cooperation can shape a bright future for science and technology. Through science, we can build bridges, strengthen competitiveness, and accelerate the green and digital transitions.

Giving the gift of negative data 🥰 Since I joined @lsajournal.org I have handled several stellar manuscripts that report null results. These papers do very well and reviewers almost always appreciate them! Thanks @rockefeller.edu news team for the article! www.rockefeller.edu/news/38829-t...

Happy to see this out 🥳 authors.elsevier.com/sd/article/S... #TeamTomo #CryoET #CryoEM

Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET

Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor pubmed.ncbi.nlm.nih.gov/41330901/ #cryoem

Preprint alert! It is my great pleasure to announce the first manuscript from the lab, a story that started @gmivienna.bsky.social and was mainly accomplished by the intrepid @gesahoffmann.bsky.social at @mpi-mp-potsdam.bsky.social. A brief thread with our findings www.biorxiv.org/content/10.1...

Yeah super cool! Look at these beautiful cells, tons of eukaryotes 🦠 images using expansion microscopy 🔬 What's next? Expand the world 🌎 ?! Congrats !

We’re back with the next DinoSphere Online Seminar! Join us on Nov 4th, we're hosting: Edmée Royen (ULiège) - Symbiodinium
 Nicolas dos Santos Pacheco (Cambridge) - Perkinsids 📅 Tue, Nov 4 - 4 PM CET/3 PM GMT 
💻 Zoom link: sites.google.com/view/dinosphere
 Please spread the word! #protistonsky

Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social! Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬. Huge thanks to the DCI for making the event possible! See you next year! 👋

✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo 🔗 www.biorxiv.org/content/10.1...

Check this out! The mysterious widespread CAGE protein complex found by my colleague @computingcaitie.bsky.social ! Cool stuff there 🥨 Shows you how much we still can and have to discover !

I had a wonderful time in Japan 🗼! Huge thanks to @ken-wakaba.bsky.social for inviting me to Sangyo University in Kyoto. Thanks to Takashi Yamano (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting! Great science and discussions!

Mitochondria finally made the headlines, yay! Oh wait, ... oh no ....

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Very cool! How do cytosolic ribosomes discriminate between ER and mitochondria targeted proteins? NAC recognizes mitochondrial targeting sequences! Very important since over 95% of the mitochondrial proteome is translated in the cytosol and then imported to mitochondria. Congrats!

A step towards easier control over mitochondrial gene expression: Silencing mitochondrial gene expression in living cells | Science www.science.org/doi/10.1126/...

Cell-to-cell connections in plants 🌿! Using cryo-ET 🔬 to have a look at plasmodesmata. Congrats Marcel, Matthias and co! You managed to push it far beyond what I thought was possible !

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Cool paper from cool people 😎 Expand all the things!

Curious about dinoflagellates? Tiny water bugs with super cool cellular architecture? Check out this cool initiative from my colleague @manondemulder.bsky.social and her friends! Their 1st online seminar is next month, don't miss it!

Re-redeading this one 📖. Great book for evolution and bioenergetics aficionados. The view is a bit distracting though ⛵

Happy to present the lab latest work, today from the green-side. The ultimate PSII assembly intermediate that directly precedes incorporation of the Mn4O5Ca cluster and formation of the OEC. Psb32 binds to PsbV, challenging the assumption the extrinsic subunits all associate spontaneously in-vivo.

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Also want to highlight that the script is based on the awesome aretomo3torelion5 from @phaips.vd.st 🤩 github.com/Phaips/areto...

To all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging: github.com/Chromatin-St... #Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Check out the latest version of MemBrain, spearheaded by computation superstar @lorenzlamm.bsky.social ! It can segment, pick particles and give you metrics on everything!

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

From my colleague @cellarchlab.com and his lab at the @biozentrum.unibas.ch I continue to be amazed by what cyro-electron tomography can do...

Congratulations Florent and coauthors!!! I'm very excited to continue diving into diversity and beauty of mitochondrial respiratory complexes in algae 😍