Online Now: Toward community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii Online now:

Japan is joining Horizon Europe, the EU’s flagship research and innovation programme. Openness and international cooperation can shape a bright future for science and technology. Through science, we can build bridges, strengthen competitiveness, and accelerate the green and digital transitions.

Giving the gift of negative data 🥰 Since I joined @lsajournal.org I have handled several stellar manuscripts that report null results. These papers do very well and reviewers almost always appreciate them! Thanks @rockefeller.edu news team for the article! www.rockefeller.edu/news/38829-t...

Happy to see this out 🥳 authors.elsevier.com/sd/article/S... #TeamTomo #CryoET #CryoEM

Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET

Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor pubmed.ncbi.nlm.nih.gov/41330901/ #cryoem

Preprint alert! It is my great pleasure to announce the first manuscript from the lab, a story that started @gmivienna.bsky.social and was mainly accomplished by the intrepid @gesahoffmann.bsky.social at @mpi-mp-potsdam.bsky.social. A brief thread with our findings www.biorxiv.org/content/10.1...

Yeah super cool! Look at these beautiful cells, tons of eukaryotes 🦠 images using expansion microscopy 🔬 What's next? Expand the world 🌎 ?! Congrats !

We’re back with the next DinoSphere Online Seminar! Join us on Nov 4th, we're hosting: Edmée Royen (ULiège) - Symbiodinium
 Nicolas dos Santos Pacheco (Cambridge) - Perkinsids 📅 Tue, Nov 4 - 4 PM CET/3 PM GMT 
💻 Zoom link: sites.google.com/view/dinosphere
 Please spread the word! #protistonsky

Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social! Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬. Huge thanks to the DCI for making the event possible! See you next year! 👋

✨Excited that the main project of my PhD is now available as a pre-print on #bioRxiv Here, we used #CryoET to visualise mitochondrial proteostatic stress and together with SPA #CryoEM shed light into the functional cycle of the Hsp60:10 chaperone system. #TeamTomo 🔗 www.biorxiv.org/content/10.1...

Check this out! The mysterious widespread CAGE protein complex found by my colleague @computingcaitie.bsky.social ! Cool stuff there 🥨 Shows you how much we still can and have to discover !

I had a wonderful time in Japan 🗼! Huge thanks to @ken-wakaba.bsky.social for inviting me to Sangyo University in Kyoto. Thanks to Takashi Yamano (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting! Great science and discussions!

Mitochondria finally made the headlines, yay! Oh wait, ... oh no ....

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

Very cool! How do cytosolic ribosomes discriminate between ER and mitochondria targeted proteins? NAC recognizes mitochondrial targeting sequences! Very important since over 95% of the mitochondrial proteome is translated in the cytosol and then imported to mitochondria. Congrats!

A step towards easier control over mitochondrial gene expression: Silencing mitochondrial gene expression in living cells | Science www.science.org/doi/10.1126/...

Cell-to-cell connections in plants 🌿! Using cryo-ET 🔬 to have a look at plasmodesmata. Congrats Marcel, Matthias and co! You managed to push it far beyond what I thought was possible !

New preprint: In situ Architecture of #Plasmodesmata. Using #cryoET, #AlphaFold & proteomics, we uncover the native organization of plant intercellular nanopores. 🔗 doi.org/10.1101/2025... 🧪🧵1/n #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM

Cool paper from cool people 😎 Expand all the things!

Curious about dinoflagellates? Tiny water bugs with super cool cellular architecture? Check out this cool initiative from my colleague @manondemulder.bsky.social and her friends! Their 1st online seminar is next month, don't miss it!

Re-redeading this one 📖. Great book for evolution and bioenergetics aficionados. The view is a bit distracting though ⛵

Happy to present the lab latest work, today from the green-side. The ultimate PSII assembly intermediate that directly precedes incorporation of the Mn4O5Ca cluster and formation of the OEC. Psb32 binds to PsbV, challenging the assumption the extrinsic subunits all associate spontaneously in-vivo.

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Also want to highlight that the script is based on the awesome aretomo3torelion5 from @phaips.vd.st 🤩 github.com/Phaips/areto...

To all #ChlamyDataset enthusiasts, here is a script that lets you directly import the pre-processed results from EMPIAR-11830 into #RELION v5 for subtomogram averaging: github.com/Chromatin-St... #Chlamydomonas #TeamTomo #CryoET #OpenSoftwareAcceleratesScience

1/5: Over the last few months, I have been working on an update for the #FollowRelionGracefully dashboard, which offers improved real-time job previews for #cryoEM #Relion jobs.

Check out the latest version of MemBrain, spearheaded by computation superstar @lorenzlamm.bsky.social ! It can segment, pick particles and give you metrics on everything!

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

From my colleague @cellarchlab.com and his lab at the @biozentrum.unibas.ch I continue to be amazed by what cyro-electron tomography can do...

Congratulations Florent and coauthors!!! I'm very excited to continue diving into diversity and beauty of mitochondrial respiratory complexes in algae 😍

Out just now @naturecomms.bsky.social: using cryo-EM and cryo-electron tomography, we provide evidence that structurally heterogeneous ribosomes can cooperate in protein synthesis in bacterial cells: www.nature.com/articles/s41...

Attention mitochondriacs! Mitochondrial supercomplexes by cryo-electron tomography. How can you not be blown away by how beautiful this is? Fascinating that ATP synthase is largely organized as dimers since this is one of the putative configurations of mPT pore. www.science.org/doi/10.1126/...

Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6

I'm super happy that our story is now published! 📖 www.science.org/doi/10.1126/... But what changed compared to the original preprint? Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.

🔬⚡🦠 #Biologists have observed the proteins that generate #cellular energy with unprecedented resolution directly within the cell. The SNSF-funded study was conducted by @florentwaltz.bsky.social and his colleagues from the @unibas.ch and featured on the front page of #Science. ➡️ buff.ly/aUR6uf6

🔬⚡🦠 Des #biologistes ont observé avec une résolution inédite et à même la #cellule les protéines produisant l’énergie cellulaire. L’étude, menée avec le soutien du FNS par @florentwaltz.bsky.social et ses collègues de @unibas.ch , a fait la Une de #Science. ➡️ buff.ly/n2CpEbv

We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @science.org 🎉 You can find the full story here www.science.org/doi/10.1126/... @verenaresch.bsky.social did a wonderful job on the cover and animation, she really brought it to life 👩‍🎨

Weeeeeeeee (lift out)

This is the kind of story I enjoy reading! Congrats to the authors 🦠!

Running some experiments in Lausanne. The view never disappoints

To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Congratulations Marcel, Matthias & co! Remarkable technical achievement. At the EMBL tomo meeting, we've seen vitreous mouse brain biopsy, but plants 🌱 are more challenging because they are full of water 💧

Our methods paper made it to bioRxiv: doi.org/10.1101/2025... Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5 #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM

💡 At a joint press conference, the #SNSF and its ERI partners called on the Federal Council to abandon the planned cuts to #education, #research and #innovation in the "relief package 27", as they would have far-reaching consequences for the #economy and society. ➡️ https://buff.ly/3CTR2AM