Come join us at the @biozentrum.unibas.ch as Professor in Climate Biophysics. Blue-sky research, inspiring colleagues, and generous support.

Happy to help out a bit on this cool study about a membrane-remodeling protein that enables CO2 delivery to Rubisco at the center of the Chlamy pyrenoid, powering efficient carbon fixation 🌍. Great #CryoET work by Hao and Martin — Welcome to #TeamTomo!! ❄️🔬 🧪

The algal pyrenoid fixes ~1/3 of global carbon, relying on a unique membrane-within-condensate architecture. How is this architecture built? We discovered that the protein MITH1 draws membranes into the pyrenoid condensate by promoting wetting! 🌿💧 www.biorxiv.org/content/10.6...

🚨 New in @natmicrobiol.nature.com! We reveal how the antibiotic target PBP1b fortifies the E.coli division site against osmotic rupture. Proud this was completed in our independent labs @dmf-unil.bsky.social, with @avettiger.bsky.social. Congratulations to all authors! 🦠❄️🔬 #teamtomo bit.ly/3SCbOg1 👇

Super excited to launch this search for a new endowed professor at the @biozentrum.unibas.ch! Join us to build a research cluster in Climate Biophysics! 🌍🌊🌾🔬🧪 This is a unique opportunity to create a community in Basel🇨🇭working together on this timely topic! ⏳🌡️ Questions? Please reach out! 👩‍🔬👨‍🔬

Really hyped to share our pre-print on mtDNA replication-fission ! @nanigrotjahn.bsky.social (doi: doi.org/10.64898/202...) 🧵with details below ⤵️

The Chlamy chloroplast RNA polymerase paper is here!🤩 It's a beautiful and interesting structure, which raises some interesting questions about evolution. Congrats to @sramundo.bsky.social, @schullerjm.bsky.social, and all your rad peeps for getting this done 💪 #plantscience #cryoEM 🌾🔬🧪

Beautiful chromatin confetti 🧬🎊 from gently lysed archaea cells. Definitive proof for the in vivo relevance of hypernucleosomes, with more compacted chromatin during stationary phase. This is big for chromatin evolution! Go Sveta & gang! You are trailblazers!! #TeamTomo #ArchaeaSky 👩‍🔬🧪

We’re delighted to welcome @computingcaitie.bsky.social to the FMI next year! Her work will bring an interdisciplinary perspective spanning structural biology, quantitative imaging and computational analysis to our institute, opening exciting new ways to study ciliary structure and dynamics.

I will be hiring at all levels so if you are interested and want to learn more, please get in touch! 📻 ❄️ 🔬 🦠 🧬 #cilia #cryoet #proteomics #exm #protistsonsky

YAY CAITIE!! So many feels right now🥲🤗🥳. I feel so lucky you joined us, so grateful for your generous collaboration and mentorship, so proud of your accomplishments, and so excited to gain an awesome new #TeamTomo colleague here in Basel. Honestly just so in awe— you rock! Here’s to the future! 🧪👩‍🔬 🚀

Cryo-ET gives unparalleled views of cellular structures, but targeted sample preparation by cryo-FIB milling has been a constraint. No longer! A new workflow yields close to 100% targeting success for centrioles & other small structures. Read the preprint ⤵️ www.biorxiv.org/content/10.6...

Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization? I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures. tinyurl.com/localmembrane

Beautiful expansion microscopy of the Chlamy pyrenoid by our friends in the Jonikas lab. Check out that ultrastructural detail on the tubule membranes! 🔬🧪

Jon Chen @jonchenjk.bsky.social and Claris Chong's review of recent in situ cryo-ET chromatin studies and our opinion on an important future genomic direction is now published in Current Opinions in Structural Biology. www.sciencedirect.com/science/arti...

Gold Medal🏅 for the dynamic duo of cellular evolution!! Congrats @gautamdey.bsky.social & @dudinlab.bsky.social —you are explorers! It's been such a pleasure interacting with you these years on high-res imaging, environmental sampling @embltrec.bsky.social, and climate. Here's to more adventures! 🧪🌍

Vitrification ❄️🧊 is still a real bottleneck for #TeamTomo. Nice to see this initiative, and I hope some clever new developments are enabled by it 🧪

“the best way to work with cryoET data is to engage with it directly, […] build familiarity with what is actually there. As tempting as it sometimes is to lean in to automation entirely, we cannot do visual proteomics by relying blindly on automation.” mgflast.github.io/easymode/use...

Check out these two interesting ECR talks about the crazy and diverse world marine algae #ProtistsOnSky 🪸🌊🔬🧪

And now it's out! Happy that PNAS selected for the cover this SEM image snapped by @samjlord.bsky.social, one of the most evocative visualizations of Euplotes that I have ever encountered. www.pnas.org/doi/10.1073/... Stay tuned for what is shaping up to be some fascinating follow-up in the lab...

Such a fun meeting 🧪. Big thanks to @jcellsci.bsky.social and the organizers for bringing together this exciting group of “cell visualizers”. So much amazing #microscopy 🔬across cellular and molecular scales. My eyeballs were glued to every presentation 👀 🤩!!

It's out! 🥳 In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET. 📝 Paper: www.nature.com/articles/s41... 🧵 Thread: bsky.app/profile/madi... #teamtomo #proteomics #AlphaFold

Congrats Daan and Maria!!! It was an absolute pleasure riding along on your PhD journey the past years. Time for the next step 🧪🚀

MissAlignment is here to align all our Bananas🍌 and Birds🦅 into beautiful Pineapples🍍 ...Did that make sense? Either way, read Dimitry's thread🧵 for a vivid visual demonstration. This new software cooks! Look how crispy it made the #ChlamyDataset nucleosome👇. #TeamTomo is hitting escape velocity 🚀 🧪

MissAlignment is out!!! Our new software that improves reference-free tilt series alignment by mimicking human intuition. Check out Dimitry's beautiful thread for a quick summary! And the preprint for more details: www.biorxiv.org/content/10.6... The program: github.com/warpem/miss-...

I want it all; tomos BIG and SMALL And I'm bawlin everytime I stop and talk to y'all #TeamTomo 🧪 🔬

Several state-of-the-art deep-learning methods which aim to improve the interpretability of cryo-ET reconstructions are evaluated #DeepLearning #CryoElectronTomography #ContrastEnhancement doi.org/10.1107/S205...

Happy to see the preprint of our VULCROM setup online! Thanks to our amazing collaborators we've managed to get some pretty cool cryo-CLEM images by combining cryo super-resolution and cryo-ET: doi.org/10.64898/202...

New preprint 🚀 ❄️ We developed Autogrid-compatible HPF planchettes that enable direct freezing of clipped EM grids and streamline integration with cryo-FIB workflows ✔️ improved grid stability ✔️ reduced handling ✔️ robust vitrification (🦠 🧫 🧠) A step toward routine HPF for complex samples #teamtomo

Out now in Nature Communications! We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky www.nature.com/articles/s41...

Looks great! Time to CLEM all the things! 🔬 🧪 How feasible would it be to integrate this cryo-superres setup into a FIB to track tagged features of interest while milling? #TeamTomo

Excited to share our paper about copick, a dataset API and toolkit for collaborative annotation and analysis of #cryoET data! Whether you're picking particles or curating segmentations, copick reduces friction and brings #OME-Zarr to cryoET without breaking pipelines. 🧵👇 doi.org/10.1002/pro.70578

Crazy biochemistry is the norm for @schullerjm.bsky.social, but here I wanna say I have witnessed up close the amazing effort from @tomaspascoa.bsky.social and @tamb-o.bsky.social to chase the BCRII complex in situ 🤩 Big congrats to Lena Appel and @rnfr2d2.bsky.social for the cryoEM structure too 👏🏽

Amazing paper. A megadalton enzyme complex that: - combines both electron bifurcation AND confurcation steps - has a new type of iron-sulfur cluster - a new kind of weird electron transfer path - super charges electrons to reduce aromatic rings - also powers respiration 😳

Thanks for the fun collaboration into this fascinating “oil eating” biomachine. I learned a lot! 🧪 #TeamTomo

Happy to present our latest story: the BCRII complex with the Boll and @cellarchlab.com . Months of processing by @rnfr2d2.bsky.social. Also @tomaspascoa.bsky.social and @tamb-o.bsky.social managed an in-situ tour de force. And Lena Appel did amazing biochemistry. This story has it all. Enjoy it.

Across scales from cells🦠 to atoms⚛️ – We reveal how anaerobic #bacteria break down very stable aromatic compounds found e.g. in oil spills 🛢️ #Bioremediation #TeamTomo 🧪 🧶🧬 🔬 Awesome collab with Lena, Matthias, @schullerjm.bsky.social @rnfr2d2.bsky.social @tomaspascoa.bsky.social @tamb-o.bsky.social

The cartwheel was first visualized ~70 years ago in the symbiont Trichonympha in the wood termite gut. We’ve discovered the cartwheel’s secret: a unique zigzag stacking of SAS-6 tetramers that facilitates the iconic 9-fold symmetry of cilia. #cellbiology #teamtomo #SAS6 #cartwheel

Check out this cool integrative structural biology story from my colleague @computingcaitie.bsky.social on mammalian cilia 🍟 Beautiful tomos of the ciliary base !

Beautiful work from @computingcaitie.bsky.social and colleagues, showing the power of combining multiple approaches to studying complex in situ cell biology!

Crazy things have been happening in cilia world, catch up on them in Caitie's new paper! 🪱

@cellarchlab.com The soundtrack was missing 😂

This is why I’m not allowed in the wet lab anymore 🥼 🕺. My technique is too effective! 🤪 #PILife #TeamTomo 🧪

Now THIS one was quite the journey. I'm beyond happy to see this work out in the world 🥹 Huge congrats to @computingcaitie.bsky.social and also to @hugovdhoek.bsky.social and everyone else involved ♥️ with @centriolelab.bsky.social @stearnslab.bsky.social @cellarchlab.com #Cilia #CryoET #TeamTomo

It’s done!! Molecular architecture of the ciliary base in mammalian multiciliated cells! A study 13 years in the making 👴, thanks to collab with @stearnslab.bsky.social + @centriolelab.bsky.social & visionary work by @computingcaitie.bsky.social, who integrated native #cryoET with #XLMS & #UExM 🧪🧶🧬🔬

This is super impressive and interesting #TeamTomo work by @carter-lab.bsky.social. Congrats everyone!! 🧪 🧶🧬

#TeamTomo if you want to use CTF-corrected 3D subtomos from AreTomo3 in RELION v.5.1 you can use this updated script: github.com/Phaips/aretomo3torelion5

Before knowing anything else about this, I am excited because prediction of stoichometry is also an implicit prediction of allostery.

New protein complex stoichiometry prediction tool from talent PhD student @daniil-litvinov.bsky.social, check out the thread below!

Check out this awesome work from @daniil-litvinov.bsky.social: Protein complex stoichiometry prediction (both homomers and heteromers) from sequence, with some nice ablations showing what makes the difference!