ER-associated single-capped vaults are surprising and really cool!🤩 Some even contain ribosomes. Vault function is still mysterious but a role in protein quality control seems plausible. Also great to see Dicty in the #TeamTomo spotlight! #ProtistsOnSky @kgeissler.bsky.social @becklab.bsky.social 🧪

Another #notTHECover unfortunately. But this gorgeous, Tron-like vibe, drawn by the amazing @munafomarzia.bsky.social for our recent #ExM work with @gautamdey.bsky.social & @centriolelab.bsky.social will still be printed out in the lab. Read here: www.cell.com/cell/fulltex...

EMPIAR-11830 #ChlamyDataset #TeamTomo 🧪 www.cell.com/molecular-ce...

Really excellent work by the bluesky-less Marie and Sara +coauthors in our lab @embl.org! Check it out for cool combinations of light microscopy and #cryoET, and if you're interested in how yeasts reorganize structurally in response to stressors. #teamtomo

A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social. Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...

Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social Huge collaborative effort! So glad to see the community already using it to develop new resources & tools. Check it out here: shorturl.at/z4i4c #CryoEM #CryoET

Happy to see IsoNet2 performing so well on the #ChlamyDataset EMPIAR-11830. Congrats to the authors really great work. However, I have to say this tomogram looks amazing because the lamella is way too thin (~50 nm) and not vitreous! 🫠 www.biorxiv.org/content/10.6...

Holy cow the Zhou lab at UCLA is cooking with Isonet 2 - www.biorxiv.org/content/10.6... Averaging-free direct visualization of ribosome translational state! #teamtomo #cryoet

Postdoc opportunity with Michel Goedert and myself to work on #cryoEM/#cryoET structures of #amyloids. Feel free to email me with any questions you might have. mrc.tal.net/vx/mobile-0/...

🥳 Our new paper on C2CD3 is out in @plosbiology.org With the labs of @cellarchlab.com , @chgenoud.bsky.social , and @stearnslab.bsky.social , we revealed the mammalian centriole Distal Ring architecture by combining #UExM and in situ #CryoET. Big congrats to @ebertiaux.bsky.social and all involved.

Thrilled to share that my postdoc paper has just been a published in @plosbiology.org ! Thank you to all co-authors, and a special thanks to Paul and Virginie @centriolelab.bsky.social for their support throughout this work. Proud of what we achieved together! journals.plos.org/plosbiology/...

Happy to share our fun team-up with @centriolelab.bsky.social & @stearnslab.bsky.social to reveal the architecture of the mammalian centriole’s Distal Ring 🛟. Very cool multiscale integration of #UExM 📏🔬with #CryoET ❄️🔬. Congrats to @ebertiaux.bsky.social & @computingcaitie.bsky.social #TeamTomo 🧪

What an honor to see @attychang.bsky.social’s work featured in @jcb.org The Year in Cell Biology 2025 collection! Even more special that it coincides with my first #cellbio2025 conference 🙂 s/o to co-authors @baradlab.com @hamid13r.bsky.social @zidlab.bsky.social #teamtomo #cellbio25

CryoET of microbes inside an animal organ? Yes it’s possible! Check out our new preprint showing how we did it! What an amazing collaboration with amazing scientists who made this possible!

Great talk (and work 🧪 ) by @computingcaitie.bsky.social from @biozentrum.unibas.ch about exploring multiciliated cells with a complementary trio of structural techniques! Looking forward to sharing the preprint. Thanks for the fab collab @centriolelab.bsky.social @stearnslab.bsky.social #TeamTomo

Each such discovery is a game changer for our understanding of eukaryote evolution, and this paper is no exception. Meet Solarion, which displays yet again novel types of subcellular structures. Congrats to all authors on a fascinating story. #protistsonsky www.nature.com/articles/s41...

It was a joy to spend some time with you Philippe! Thanks for visiting and sharing your cool IFT Train research with us! 🚂🚂🚂🧪

New Title Alert: Icecream- a self-supervised framework for cryo-ET reconstruction that integrates equivariance principles from modern imaging theory into a deep-learning architecture. Learn more here: buff.ly/bdepEUm #SBGrid #SBGridSoftware #StructuralBiology

Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁‍♀️💎✨VIP section💅🪩🥂 Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics. s/o to #teamtomo #cryoET co-authors @hamid13r.bsky.social @attychang.bsky.social @baradlab.com

Very pretty #SciArt 🧪 🎨 render of Asgard chromatin 🧬 (determined by the @dodonova-sveta.bsky.social lab). Nice one Leonora! 👩‍🔬👩‍🎨

Beautiful systematic analysis of membrane thickness inside native cells. This is a great use of both the Chlamy Dataset (bsky.app/profile/cell...) and #MemBrain segmentations (bsky.app/profile/lore...). The open-source workflow is a very helpful resource for #TeamTomo. Thanks and congrats!! 🧪 🔬

Always happy to see the #ChlamyDataset put to good use 😉

Hey #TeamTomo 🧪🔬, ICECREAM is getting tastier! Please give it a try on your tomograms and send us feedback so we can get the recipe just right 🍦😋

Very thorough work that applies cryo-ET to measure bilayer thickness. Surprisingly, the missing wedge is not a big problem!

Check out #DinoSphere 🦖🪩 -- Early Career Researchers sharing their latest insights into dinoflagellate biology. Online and free. Tune in! 👩‍🔬📺 #ProtistsOnSky 🧪🌊 🌍 🔬

Intriguing structures of Asgard hypernucleosomes in open and closed conformations provide a glimpse into the evolution of chromatin from our closest prokaryotic relatives. Bold and eye-opening work by the @dodonova-sveta.bsky.social lab! Also great visualization 🧪👩‍🔬🎨 #CryoEM #ArchaeaSky

REMINDER! One week left to apply for what (in my humble and somewhat biased opinion) is one of the best academic jobs on the planet! Come join our vibrant and collaborative community here at the @biozentrum.unibas.ch @unibas.ch 🧪

Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social! Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬. Huge thanks to the DCI for making the event possible! See you next year! 👋

A class like no other!! From #AI structure hallucination 🤖 to #CryoEM structure reality 🔬 by @biozentrum.unibas.ch @unibas.ch undergraduate students👩‍🔬👩‍🎓 in just a few weeks! One of their creations is this beautiful flower-shaped tetrameric pore 🌼, a brand new member of the #ProteinCosmos 🧶🧬 🧪

Hey #TeamTomo …would you like to try some 🍦ICECREAM!? Our @unibas.ch colleagues over at the @ivandokmanic.bsky.social lab have developed a stunningly effective tool to de-noise and de-wedge your tomograms. The contrast and details will give you brain freeze! 🍨❄️🤯 Please try, we need feedback 🧪🧶🧬🔬

Icecream 🍨is open source and super easy to instaIll and run. It is already our favorite tool for denoising in the @cellarchlab.com, but it's under active development and we would love to hear your feedback! Please give it a try and let us know: github.com/swing-resear... #TeamTomo

Sounds like fun to deposit 140 subtomogram averages 😳

So, we all know that VIPP1 (the Very Important Protein in Plants) is the "photosynthetic ESCRT-III", required for the biogenesis and maintenance of light-harvesting thylakoids. But what about ESCRT-II, you say? ...Meet VIA1! Congrats @mayankdu.bsky.social on your lab's first paper! #PlantScience 🧪 🌾

Let’s stare into the far red 🏮. A stunning tour-de-force of high-resolution #CryoEM by Gio, Bill, & company reveals how the machinery of photosynthesis redshifts itself so cyanobacteria can live on far red light, deep in the cracks of rocks or under microbial mats. #PlantScience 🧪 🧶🧬 🌾 🔬 🌍

The KEY🔑 to regulating Rubisco phase separation in the Chlamydomonas algal pyrenoid. Beautiful work by Shan He, @mjonikas.bsky.social, and friends. #PhaseSeparation #CO2 #PlantScience 🧪 🧶🧬 🌾 🌍

What happens when the cell's powerhouse gets stressed out? 💪🥵 Great visualization in this #Mitochondria #TeamTomo study! Congrats @denkenny.bsky.social @ruferbus.bsky.social & crew 🧪 🧶🧬 🔬

Hellooooo beautiful!😍 #CryoEM #ArchaeaSky 🧪

#TeamTomo ❄️🔬 meets #MassSpec Imaging 🧬🔬. Super cool, and great potential for EM grids with mixes of different cell types. For example -- environmental samples! @embltrec.bsky.social 🌍 🧪

So much fun to host @edwardmarcotte.bsky.social at the @biozentrum.unibas.ch and introduce him to the @cellarchlab.com, thanks for a wonderful Discovery Seminar!!

A place with fantastic colleagues, excellent facilities, a stimulating scientific environment, and a very international and vibrant city - it's got it all!

Biocenter is THE BEST!

Come join us in Basel! @biozentrum.unibas.ch is an amazing place to start your group.

Yay @centriolelab.bsky.social !! 🙌

EXCITING NEWS!! We're searching for a new professor colleague at the Biozentrum🇨🇭. Ideal candidates are exploring novel biological questions with structure, biophysics, or high-resolution imaging. 🧪 🧶🧬 🔬 The call closes Nov 3. Please get in touch if you would like more information. Come join us! 👩‍🔬🤓

The former supervisor of @computingcaitie.bsky.social is coming to visit and tell us all about structural proteomics. Looking forward to this #DiscoverySeminar from @edwardmarcotte.bsky.social !! 😃 #ProteinCosmos 🧶🧬

The case for studying proteins inside the cell 😉 #CryoET #TeamTomo

Love it! 🧪🤓 Ya know, thylakoids can also play this paracrystalline lattice game. In plants, they emerge from the prolamellar body during de-etiolation 🟡➡️🟢 Little piece by @wojwie.bsky.social & me: www.nature.com/articles/s41... Lovely review by Andrew Staehelin💚: link.springer.com/article/10.1...

An interesting parallel: Rod outer-segment disk membranes are highly packed with a membrane protein (rhodopsin) 👁️, similar to the stacked domains of thylakoids (PSII-LHCII) 🌱. And isolation of disk membranes may cause rhodopsin to crystallize into 2D arrays 💠 www.nature.com/articles/421... 🧪 🧶🧬🌾🔬

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on! #TeamTomo #PlantScience 🧪 🧶🧬 🌾 elifesciences.org/articles/105... 1/🧵

This is the team-up I was waiting for 💪💪! Very cool #TeamTomo ❄️🔬 collab from @cellforganized.bsky.social & @shyamalm.bsky.social, showing how to spatially partition organelles in E. coli: from #CO2 -fixing carboxysomes to biomolecular condensates. Big potential for #SyntheticBiology 🧪🧶🧬🌾