Happy to see this out 🥳 authors.elsevier.com/sd/article/S... #TeamTomo #CryoET #CryoEM

If you want to learn how to process cryo-ET data using the Chlamy dataset don't forget to check : tomoguide.github.io @phaips.vd.st

Come join us! Soon I will be advertising a postdoc vacancy in my group as part of my @erc.europa.eu AdG project 'DARK ROOTS'. Focus of the project will be on phylo- and metagenomic mining of novel prokaryotic lineages. I will soon post a link here - stay tuned, and please repost! #asgardarchaea

Starting at 7pm Central I will be streaming from the bottom of the ocean off the coast of Uruguay! Feel free to join and ask questions. www.youtube.com/@SchmidtOcean

An Asgard archaeon with internal membrane compartments Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors! www.biorxiv.org/content/10.1...

Very happy that NWO supports this major upgrade of our national EM infrastructure. It is great that this initiative got broad support all over NL, and it will EMPower fantastic science - looking forward to that! www.uu.nl/en/news/33-m...

Thrilled, and admittedly relieved, to see this major investment in Dutch #cryoEM become reality! 🇳🇱🔬 Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠 www.uu.nl/en/news/33-m...

We’re back with the next DinoSphere Online Seminar! Join us on Nov 4th, we're hosting: Edmée Royen (ULiège) - Symbiodinium
 Nicolas dos Santos Pacheco (Cambridge) - Perkinsids 📅 Tue, Nov 4 - 4 PM CET/3 PM GMT 
💻 Zoom link: sites.google.com/view/dinosphere
 Please spread the word! #protistonsky

💡🔬 TECHNICAL INNOVATION 💡🔬 Neubauer et al. developed a Nostoc reporter strain enabling high-throughput quantitative monitoring of hormogonia differentiation in cyanobacteria as a response to biotic and abiotic factors. 🔗 doi.org/10.1093/jxb/... #PlantScience 🧪 @peterszovenyi.bsky.social

🥨🔬!

🌱 Using ‘compelling’ methods, including #CryoET, researchers mapped spinach thylakoid membranes at single-molecule precision, revealing how photosynthetic complexes are organised and settling long-standing debates on chloroplast architecture. buff.ly/j3TSIkn

New short paper from our lab @currentbiology.bsky.social, in which we discover of a new mode of cell motility for choanoflagellates: flagellar gliding. www.cell.com/current-biol... - A 🧵

A new #UExM probe for #Actin! Fun times thinking/developing/testing with @centriolelab.bsky.social & @lreymond.bsky.social ! Congrats to all ! If interested in #UExM, or #HAK-Actin, feel free to reach out ! Soon available at @spirochrome.com #Expansion #Microscopy #ProtistsOnSky

Our new preprint with a new #Asgard from stromatolites (and its Desulfobacterota companion). Complete circularised genomes of both, cool #cryoem images, protein modelling, etc Big group effort with a lot of people involved #archaeasky #archaea www.biorxiv.org/content/10.1...

Please Re-Post. Our global GRUMP microbial ocean database is out in Scientific Data! Unfractionated, single universal PCR, with Archaea, Bacteria, and Eukaryotes all on the same scale with the same denominator. Pole to pole, depths to 6000m. Lots of metadata. www.nature.com/articles/s41...

After 5 amazing years @pilhoferlab.bsky.social I'm starting my lab @embl.org Grenoble this November embl.org/wollweber We'll image Asgard archaea and many other strange microbes ( #archaeasky, #protistsonsky..) to understand eukaryogenesis First job ad: #teamtomo scientist!🔬❄️ tinyurl.com/2rdu2ze6

Excited to share the lab's 1rst preprint! Rapid high-res immunofluorescence is now possible in cultured & environmental diatoms thanks to 4-fold expansion microscopy. A step-change for comparative cell biology in one of the most important phytoplankton groups on the planet 🥳 tinyurl.com/m9s5su7s 1/2

Final PhD paper now reviewed and published in JSB:X. I was happy with some great reviews that improved the paper! Thanks to Sander Roet for his help with the code base, and Remco Veltkamp and @fridof.bsky.social

Are you a recent dinoflagellate convert, or already long dedicated to the craft? Don't you wish you could talk about them more? Join Manon's DinoSphere seminar series! All things phylogeny, imaging, ultrastructure and more :) #protistsonsky

Unicore identifies single-copy protein structures across genomes using Foldseek, bypassing slow structure predictions by utilizing 3Di predictions from ProstT5, enabling rapid phylogenetic inference at the tree-of-life scale. 1/n 📄 www.biorxiv.org/content/10.1... 💾 github.com/steineggerla...

Anti-gatekeeping workflows is what keeps #teamtomo growing! Love to see it.

Check out 'the website' by Phil&Flo - a tomo processing pipeline/tutorial from frame to structure. For easy implementation of the Aretomo3 to RELION5 pipeline, or your students first dataset, get processing!

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

Join us in Hamburg! May 6: Advancing Correlative Light & cryo-EM: Bridging Molecular Precision & Tissue Architecture Register: indico.desy.de/event/48246/ May 9: Beyond the Barrier: Advanced Microscopy of Pathogens in High-Biosafety-Containment Labs Register: indico.desy.de/event/48139/...

#weekendreading🔬📑 Tissue-like multicellular development triggered by mechanical compression in archaea @science.org #microsky www.science.org/doi/10.1126/...

Very Happy to finally see this story out. I remember the first time @archaeon-alex.bsky.social showed me the videos maybe 3-4y ago on a Zoom call ! Mind blown then and still mind blown today. A must read for all.

#Mitochondria power our cells, but how? Using cryo-electron tomography, @cellarchlab.com and @florentwaltz.bsky.social report in @science.org that energy-producing proteins form supercomplexes, optimizing ATP production.

I'm super happy that our story is now published! 📖 www.science.org/doi/10.1126/... But what changed compared to the original preprint? Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.

Cryo-ET Analysis Unveils the Minimum Structural Unit and Hierarchical Ultrastructure of Silk Fibers in Silkworm and Spider www.biorxiv.org/content/10.1101/2025.03.12.642793v1 #cryoem

From cytoplasm to lumen-mapping the free pools of protein subunits of three photosynthetic complexes using quantitative mass spectrometry pubmed.ncbi.nlm.nih.gov/40077900/

1/ Why are so many scientists silent on the climate crisis? We interviewed neuroscientists @anne-urai.bsky.social & @clarekelly.bsky.social on their participatory workshops designed to stimulate scientists from awareness to activism. buff.ly/SSf99Q2

To all #TeamTomo #CryoET and #Chlamydomonas aficionados: we have updated EMPIAR-11830 with a bug fix concerning some cryo-CARE denoised tomos as well as additional files and metadata! 🎉👩🏽‍💻 www.ebi.ac.uk/empiar/EMPIA... A little thread about what's new... 🧵 1/n

We (@cellarchlab.com peeps, @manondemulder.bsky.social @phaips.vd.st @fannyleblanc.bsky.social @computingcaitie.bsky.social and Ricardo) had a blast at the EMBO in situ meeting in Heidelberg! Meeting old friends and making new ones! Lots of cool science and new ideas to try 👨🏻‍🔬🔬

Have you used #network_analyses in the past to analyse your #ecology data? Have you wondered how many of those correlations actually represent interactions between #microorganisms? Here I present a paper how we leveraged #protistsonsky to evaluate the validity of the method. A thread:

Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging | Science Advances www.science.org/doi/10.1126/...

Ok everything's going to shit, but I just screenprinted plankton in blacklight ink a la fluorescence microscopy and I'm super into it! Tried a phase contrast image too- it sorta worked. If you have any fluorescent images you'd like me to do feel free to reach out. #sciart #copepod #diatom

Screenprinting fluorescence microscopy images in blacklight ink, part II. Still need to hone the technique, but I'm looking for more images so feel free to reach out if you have any to share. Could make a nice blacklight LED frame for ya too. Cultured cell image by Derek Sung. #sciart #biology

Wait till they view it with expansion microscopy.

Excited to share our ✨preprint✨ from my work in Ted Wensel's lab! We present novel insights into the photoreceptor-specific roles of CEP290. 🧵👇 Thanks for the helpful participation from everyone involved, especially @mrobichaux.bsky.social and @agostolab.bsky.social! www.biorxiv.org/content/10.1...

What started as a visit to learn ExM by @fburki.bsky.social turned into a full blown ExM workshop, many friends & colleagues, > 25 different species, obviously challenges and some good chat around drinks. Lets see the #UExM efficieny by End of this Week. #ProtistsOnSky

Microscopy Nodes is now up on bioRxiv! 🚀 This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev). High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬 www.biorxiv.org/content/10.1...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

Niet vanwege t lekkere weer, maar Nederland is een paradijs 🌴 www.ftm.nl/artikelen/to...

Fundamental Constraints To The Logic Of Living Systems astrobiology.com/2024/12/fund... #astrobiology #biology #evolution

Check this out, maybe your last read of the year, or the first for the next one 📖? We used Chlamydomonas 🦠 to push cellular tomography towards the next step and made all the data public! BTW it's the same dataset I used for the mitochondrial respirasomes www.biorxiv.org/content/10.1... Enjoy 🥳!

I’ll have more to say about this later, but please have a look, and I hope the #TeamTomo community finds good uses for these 1829 Chlamy tomograms and annotations 🧪🧶🧬🌾🔬

Hey #teamtomo checkout our #teamscience approach to #cellbio #cryoET I can’t thank @cellarchlab.com enough for this one 😍😍 Awesome team science from @mpibiochem.bsky.social @biozentrum.bsky.social Thermo Fisher Scientific and more #firstpost on Bluesky www.biorxiv.org/content/10.1...

More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy! Check out the preprint: www.biorxiv.org/content/10.1... Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com #TeamTomo

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii biorxiv.org/cgi/content/short/2024.12.28.630444v1