Thorsten B. Blum et al.: Radiation-dose effects in correlative X-ray/cryo-electron microscopy of frozen-hydrated biological samples ##CryoXRayTomography##CryoElectronMicroscopy##CorrelativeMicroscopy@eth...##IUCrhttps://journals.iucr.org/paper?S2059798326001427

An optimized data-acquisition and processing strategy for the collection of high-quality electron diffraction data from extended crystals using conventional cryo-EM instrumentation #ElectronDiffraction #RadiationDamage #DataCompleteness doi.org/10.1107/S205...

This topical review considers a Protein Data Bank and Cambridge Structural Database data survey of crystal structures that have been determined at elevated temperatures #BodyTemperature #PhysiologicalConditions #37C doi.org/10.1107/S205...

A theoretical framework for the development of dynamic nuclear polarization neutron macromolecular crystallography to advance the resolution of H atoms within biomolecular crystals is presented #DynamicNuclearPolarization #PhaseRetrieval #Hydrogen doi.org/10.1107/S205...

Bharath Vinjamuri et al.: X-ray beam effects on metallo-peptides reflect redox events: insights from X-ray absorption spectroscopy measurements on metal complexes of truncated amyloid-β peptides @UGrenobleAlpes...##IUCrhttps://journals.iucr.org/paper?S2059798326001348

Having made the transition from primarily X-ray crystallography to cryoEM, this resource would have been invaluable.

This is a fantastic example of how to begin as a newcomer to #cryoEM. Being proactive in getting advice from experienced scientists and going to any of the great workshops would likely lower the incidences of erroneous structures in the EMDB and PDB (There’s not a lot but there are a few).

This is an interesting article for those beginning down the cryoEM route for structure determination from the perspective of a crystallography lab. Not giving up on crystallography yet 😜 but I honestly can't think of a single crystallography lab now that isn't also using CryoEM in some capacity.

Henry N. Jones et al.: Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements ##ContrastEnhancement... ##IUCrhttps://journals.iucr.org/paper?S2059798326001166

Hassan Zafar et al.: Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies ##CryoElectronMicroscopyCryoEM##StructuralBiology##SingleParticleAnalysis@uvmvermont...##IUCrhttps://journals.iucr.org/paper?S205979832600080X

Henry N. Jones et al.: Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements ##ContrastEnhancement... ##IUCrhttps://journals.iucr.org/paper?S2059798326001166

Hassan Zafar et al.: Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies ##CryoElectronMicroscopyCryoEM##StructuralBiology##SingleParticleAnalysis@uvmvermont...##IUCrhttps://journals.iucr.org/paper?S205979832600080X

A protocol has been developed to quantify and localize questionable backbone conformations in X-ray structure models, which can affect the interpretation of structural data #QuestionableConformations #CrystallographicStructures #StructuralAlphabet doi.org/10.1107/S205...

Nigel W. Moriarty et al.: Validated ligand geometries for macromolecular refinement restraints and molecular-mechanics force fields ##MacromolecularCrystallography##LigandRestraints##Refinement@UCBerkeley...##IUCrhttps://journals.iucr.org/paper?S2059798326000975

Modification of PLK1 following the surface-entropy reduction approach yielded a protein which crystallizes readily and forms an excellent new crystallization platform for PLK1–inhibitor co-crystal structures #SurfaceEntropyReduction #SER #Kinase doi.org/10.1107/S205...

Andrea Eisenreichova et al.: Phosphatidylinositol transfer protein α binds microcolins in its open conformation ##LipidTransport##CrystalStructure##Inhibitor... ##IUCrhttps://journals.iucr.org/paper?S2059798326000872

Apo and complex crystal structures of L. reuteri glycosyltransferase C reveal key sugar-nucleotide binding residues underpinning strain-specific glycosylation of serine-rich repeat proteins #AccessorySecretionSystems #Glycosyltransferases doi.org/10.1107/S205...

Using only the first few frames of data from a large number of randomly oriented crystals can improve the recorded ED intensities, the data statistics and the resolution of the final structure #MicroED #DataProcessing #MacromolecularStructure doi.org/10.1107/S205...

I'm proud to present this first article from my PostDoc: We probed the different responses of metalloproteins (myoglobin, Mb and a copper-containing Nitrite Reductase, CuNIR) to reduction of their metal centres by X-rays at two temperatures (100K, 392K-RT) with UV-vis absorption and diffraction.

I find Airlie’s composite self rotation representation much easier to interpret. Check it out!

Very excited to share my love of crystallography in this article celebrating over 100 years of X-ray methods and the Nobel Prize for BBC and the Nobel Foundation: www.nobelprize.org/stories/x-ra...

M​artin V. Appleby et al.: Modelling of radiation damage and beam-induced heating of room-temperature samples at extremely high flux MX beamlines ##RadiationDamage##MacromolecularCrystallography... ##IUCrhttps://journals.iucr.org/paper?S2052252525011224

The Xtricorder tool offers a likelihood-enhanced self-rotation function and presents a novel graphical representation, the `composite-section diagram' #MolecularReplacement #MatthewsCoefficient #MachineLearning doi.org/10.1107/S205...

CrysFormer is a machine-learning (ML)-based approach for improving predictions provided by other ML models, i.e. those in the AlphaFold Protein Structure Database, given Patterson maps #Phasing #Patterson #MachineLearning doi.org/10.1107/S205...

CryoLike is computationally efficient software for evaluating image-to-structure (or image-to-volume) likelihoods across large image data sets packaged in a user-friendly Python workflow #CryoEM #MolecularModeling #MolecularDynamics doi.org/10.1107/S205...

M​aria Cristina Burla et al.: Updating direct methods V. Phasing when triplet invariants are estimated via the Patterson map ##DirectMethods##PattersonMap##ProbabilisticApproach... ##IUCrhttps://journals.iucr.org/paper?S2059798326000732

The first ten years of the CCP-EM Spring Symposium, an annual conference to bring together the cryoEM community to present and discuss the latest methodological advances and applications, are described #Cryoem #ElectronCryoMicroscopy #CCPEM doi.org/10.1107/S205...

It’s been fun to put together and write up cryoJAX. Hooray for open-source cryo-EM software! Now it’s time to use it…

Nicolas Caramello et al.: Coupled on-line in crystallo UV–Vis absorption spectroscopy and X-ray crystallography to compare specific radiation damage in metal-containing proteins at room versus cryogenic temperature... ##IUCrhttps://journals.iucr.org/paper?S2059798326000690

February issue is now available: journals.iucr.org/d/issues/202... The cover image highlights a method to characterize cryo-EM angular view distributions quantitatively and represent them as simple one-dimensional curves rather than two-dimensional colored diagrams. More at doi.org/10.1107/S205...

Go use cryojax ! It's awesome! I can't wait to see what people do with it.

M​ichael J. O'Brien et al.: CryoJAX: a cryo-electron microscopy image-simulation library in JAX ##CryoEM##ImageSimulation##AutomaticDifferentiation@harvard@unewhaven@Cornell...##IUCrhttps://journals.iucr.org/paper?S2059798326000550

Clement E. Blanchet et al.: SASBDB reaches 5000 data sets: empowering open science and next-generation SAS analysis ##SmallAngleScattering##BiologicalDataBanks##OpenData... ##IUCrhttps://journals.iucr.org/paper?S2059798326000641

Kelly L. Parker et al.: Crystal structure of Schistosoma mansoni cathepsin D1 in complex with a nanobody reveals the conformation of the propeptide-bound state ##SchistosomaMansoni##CathepsinD##Nanobodies@UniofOxford...##IUCrhttps://journals.iucr.org/paper?S2059798326000422

Jiatai Zhang et al.: Structural study of Nicotiana benthamiana NADPH-dependent thioredoxin reductase C and its molecular mechanism of interaction with 2-Cys peroxiredoxin ##XRayDiffraction##CysPeroxiredoxin... ##IUCrhttps://journals.iucr.org/paper?S2059798326000495

Jane Richardson was born #OTD in 1941 + Developed the Richardson (ribbon) diagram to represent proteins' 3D structure (becoming a standard representation for protein structures) + MacArthur Fellow, 1985 + Elected, Nat'l Academy of Sciences, 1991 + President, Biophysical Society, 2012 #WomenInSTEM

Charles Barchet et al.: Explicit correction of severely non-uniform distributions of cryo-EM views ##CryoEM##3DReconstruction##ViewFrequency... ##IUCrhttps://journals.iucr.org/paper?S2059798326000306

Alexandre G. Urzhumtsev: Quantifying distributions of cryo-EM projections ##CryoEM##AngularDistributionOfViews##NonUniformDistribution... ##IUCrhttps://journals.iucr.org/paper?S2059798325011611

Alexander Wlodawer et al.: How to mitigate the caveat emptor burden of human and machine users of the Protein Data Bank ##MacromolecularStructureQuality##ArtificialIntelligence##DataMining@uva...##IUCrhttps://journals.iucr.org/paper?S2059798325011477

Now available: the Proceedings of the 2024 @ccpem.bsky.social em Spring Symposium, including a review of the first ten years of the Symposium: journals.iucr.org/special_issues/2025/CCPEM2024/ View all of the Proceedings at journals.iucr.org/d/services/ccpem

Now available: the Proceedings of the 2024 @ccpem.bsky.social em Spring Symposium, including a review of the first ten years of the Symposium: journals.iucr.org/special_issues/2025/CCPEM2024/ View all of the Proceedings at journals.iucr.org/d/services/ccpem

#IUCr2026 Pre-Congress Workshop: Structure Solution with Phenix Offers lectures and tutorials on using Phenix for automated macromolecular structure determination and validation from xtal and cryoEM data, integrating predicted models like AlphaFold event.fourwaves.com/...

Our January issue is out: journals.iucr.org/d/issues/202... The cover highlights a data survey of the PDB and CSD for macromolecular and ligand structures that have been determined at elevated temperatures. Read more at doi.org/10.1107/S205...

Yusuke Yamada et al.: A new macromolecular crystallography endstation at NanoTerasu for accelerating structural biology and drug discovery ##IntegratedStructuralAnalysisPlatform@TohokuUniPR...##IUCrhttps://journals.iucr.org/paper?S2059798325011234

Alaa Shaikhqasem et al.: Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals ##ElectronDiffraction##RadiationDamage##DataCompleteness@UniHalle...##IUCrhttps://journals.iucr.org/paper?S2059798325011258

Register for hands-on workshops held just before #IUCr2026 Choose from 20 workshops, including Enabling PDB Deposition of Integrative/Hybrid Methods Structures Determined using 3DEM plus Chemical Crosslinking and Other Complementary Approaches Details: event.fourwaves.com/...

New interview alert! We had a chat with Mike Cianfrocco, Scott Stagg and Jan-Hannes Schäfer about their CryoSift tool – details in the thread. www.youtube.com/watch?v=ROlc...

Alice Brink et al.: Towards patient-relevant structures: reviewing body-temperature biological macromolecules and their ligands for pharmaceutical applications ##37C##PhysiologicalConditions@UFSweb@OfficialUoM...##IUCrhttps://journals.iucr.org/paper?S2059798325010617

Congratulations to Matthew and Josh! Check out our extensible Python based 2DTM package, check out our example adaptations for optimizing template parameters and constrained searches and customize to suit your own experiments. Stay tuned for exciting future developments in the pipeline :)

M​atthew D. Giammar et al.: Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology ##InSituStructuralBiology##CryoEM##CryoET@usc...##IUCrhttps://journals.iucr.org/paper?S2059798325009982