Yusuke Yamada et al.: A new macromolecular crystallography endstation at NanoTerasu for accelerating structural biology and drug discovery ##IntegratedStructuralAnalysisPlatform@TohokuUniPR...##IUCrhttps://journals.iucr.org/paper?S2059798325011234

Alaa Shaikhqasem et al.: Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals ##ElectronDiffraction##RadiationDamage##DataCompleteness@UniHalle...##IUCrhttps://journals.iucr.org/paper?S2059798325011258

Register for hands-on workshops held just before #IUCr2026 Choose from 20 workshops, including Enabling PDB Deposition of Integrative/Hybrid Methods Structures Determined using 3DEM plus Chemical Crosslinking and Other Complementary Approaches Details: event.fourwaves.com/...

New interview alert! We had a chat with Mike Cianfrocco, Scott Stagg and Jan-Hannes Schäfer about their CryoSift tool – details in the thread. www.youtube.com/watch?v=ROlc...

Alice Brink et al.: Towards patient-relevant structures: reviewing body-temperature biological macromolecules and their ligands for pharmaceutical applications ##37C##PhysiologicalConditions@UFSweb@OfficialUoM...##IUCrhttps://journals.iucr.org/paper?S2059798325010617

Congratulations to Matthew and Josh! Check out our extensible Python based 2DTM package, check out our example adaptations for optimizing template parameters and constrained searches and customize to suit your own experiments. Stay tuned for exciting future developments in the pipeline :)

M​atthew D. Giammar et al.: Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology ##InSituStructuralBiology##CryoEM##CryoET@usc...##IUCrhttps://journals.iucr.org/paper?S2059798325009982

Now out in full form. Again, a big thanks to #teamtomo and @alisterburt.bsky.social for developing the infrastructure to build this on. journals.iucr.org/d/issues/202...

M​ario Milani et al.: Structure, substrate recognition and therapeutic targeting of the human ADAMTS-5 spacer domain ##SpacerDomain##Aggrecanase##Osteoarthritis@LaStatale...##IUCrhttps://journals.iucr.org/paper?S2059798325010290

Hui Bao et al.: The crystal structure of EpHTT, a hydroxycinnamoyl transferase from Echinacea purpurea ##EpHTT##HydroxycinnamoylTransferases##EchinaceaPurpurea... ##IUCrhttps://journals.iucr.org/paper?S205979832501023X

CCP-EM Spring Symposia have become key venues for methods-focused cryo-EM advances and an annual fixture for structural biologists worldwide. Their early adoption of online talks set the bar for inclusive meetings, and even as they have grown over the years, they have kept a family feel. Kudos!

Giles and Burnley: Ten years of the CCP-EM Spring Symposium ##Cryoem##ElectronCryoMicroscopy##CCPEM... ##IUCrhttps://journals.iucr.org/paper?S2059798325010496

I really like the new summary representation Airlie has come up with for the self rotation function!

M​cCoy and Read: Xtricorder: a likelihood-enhanced self-rotation function and application to a machine learning-enhanced Matthews prediction of asymmetric unit copy number ##MachineLearning@Cambridge_Uni...##IUCrhttps://journals.iucr.org/paper?S2059798325009647

Wai Shing Tang et al.: CryoLike: a Python package for cryo-electron microscopy image-to-structure likelihood calculations ##CryoEM##MolecularModeling##MolecularDynamics... ##IUCrhttps://journals.iucr.org/paper?S2059798325009350

The structure of NAD+-dependent methanol dehydrogenase 1 from B. methanolicus MGA3 provides essential insights for the rational engineering of methanol-utilizing biocatalysts #MethanolOxidation #MethanolDehydrogenase #TypeIIIAlcoholDehydrogenase doi.org/10.1107/S205...

Uwe Eberspaecher et al.: Polo-like kinase 1–inhibitor co-complex structures via the surface-entropy reduction approach and a DARPin-assisted approach ##SurfaceEntropyReduction##SER##Kinase... ##IUCrhttps://journals.iucr.org/paper?S2059798325009325

BCA BSG Winter Meeting 2025! Theme: "New Advances and Future Directions in Structural Biology" 🗓️ 12th Dec 2025, University of Oxford Poster session + prizes! (Talk deadline: 28th Nov) ​Register: www.oxforduniversitystores.co.uk/product-cata... ​#StructuralBiology #bsgwinter2025 #Crystallography

Hongyi Xu et al.: Limiting the effects of radiation damage in MicroED through dose selection during data processing ##MacromolecularStructure##DataProcessing@stockholms_univ@umeauniversitet@ANUmedia...##IUCrhttps://journals.iucr.org/paper?S205979832500912X

Two crystallographic fragment-screening campaigns against SARS-CoV-2 NSP1 identified 21 hits from 192 screened fragments, the highest hit rate reported for Nsp1 to date #XRayCrystallography #SARSCoV2 #CrystallographicFragmentScreening doi.org/10.1107/S205...

Calculating the FSC curve on the Å−3 scale provides a balanced distribution of Fourier coefficients per shell, yielding more statistically equivalent values than working with the conventional scale #FSCCurve #ResolutionShells #InverseCubicResolution doi.org/10.1107/S205...

Citizen scientists playing the biochemistry video game Foldit are correcting errors in the PDB to improve our public knowledgebase of protein structure #Foldit #PDBREDO #CitizenScience doi.org/10.1107/S205...

Chao-Sheng Chen et al.: Structure of the bacteriophage PhiKZ non-virion RNA polymerase bound to a p119L open promoter analogue ##CryoEM@imperialcollege@telavivuni1...##IUCrhttps://journals.iucr.org/paper?S2052252525009273

Juyeon Lee et al.: Structural insights into the antiviral efficacy of AG7404 against human rhinovirus 3C proteases ##3CProteases##Rhinovirus##AntiviralAgents@snunow...##IUCrhttps://journals.iucr.org/paper?S2052252525008929

Our November issue is available at journals.iucr.org/d/issues/202... On the cover: recent studies demonstrate that the range of samples suitable for cryo-EM single-particle analysis is expanding towards increasingly more native samples. Read the review at shorturl.at/mitjg

November issue out now: journals.iucr.org/f/issues/202... On the cover: the structure of the kinetically slow and catalytically impaired Gln143Asn mutant of human mitochondrial MnSOD leads to the observation of transient hydrogen peroxide-bound states. More at shorturl.at/41Mb9

Ryan Griffiths et al.: A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system ##CrystalStructure@uniofeastanglia...##IUCrhttps://journals.iucr.org/paper?S2059798325008782

Our November issue is available at journals.iucr.org/d/issues/202... On the cover: recent studies demonstrate that the range of samples suitable for cryo-EM single-particle analysis is expanding towards increasingly more native samples. Read the review at shorturl.at/mitjg

Jonas Moecking et al.: Cryo-EM single-particle analysis expanding towards increasingly native samples ##SingleParticleAnalysis##InSitu##CryoEM@uniutrecht@uniutrecht...##IUCrhttps://journals.iucr.org/paper?S2059798325008332

M​a and Kong: Structural studies of NAD+-dependent methanol dehydrogenase 1 from Bacillus methanolicus MGA3 ##MethanolOxidation##MethanolDehydrogenase##TypeIIIAlcoholDehydrogenase... ##IUCrhttps://journals.iucr.org/paper?S205979832500909X

The reduced unit-cell dimensions of cryo crystals of statin-bound CYP105A1 resulted in increased symmetry interactions, which induced cis–trans conversion of the C-terminal peptide bond of Pro142 #CYP105A1 #CytochromeP450 #Statins doi.org/10.1107/S205...

Major modes of behavior within low-pLDDT regions were identified through a survey of human proteome predictions provided by the AlphaFold Protein Structure Database #AlphaFold2 #StructurePrediction #ConditionalFolding doi.org/10.1107/S205...

Frank Lennartz et al.: Crystallographic fragment screening against SARS-CoV-2 nonstructural protein 1 using the F2X-Entry Screen and a newly developed fragment library ##SARSCoV2@humboldtUni@KITKarlsruhe...##IUCrhttps://journals.iucr.org/paper?S2059798325008563

Unintended proteins can easily sneak into experiments, but cryo-EM shows surprising power to uncover these `off-target' proteins in great detail #CryoEM #ArnA #AcrB doi.org/10.1107/S205...

Alexandre G. Urzhumtsev: Rescaling FSC curves ##FSCCurve##ResolutionShells##InverseCubicResolution@unistra...##IUCrhttps://journals.iucr.org/paper?S2059798325008526

Andreas C. Petrides et al.: Reconstructing biological molecules with help from video gamers ##Foldit##PDBREDO##CitizenScience@umassd...##IUCrhttps://journals.iucr.org/paper?S2059798325008149

Gayathri Yuvaraj et al.: Critical evaluation of three cryo-EM structures of particulate methane monooxygenase by quantum refinement ##QuantumRefinement##CryogenicElectronMicroscopy@lundsuni...##IUCrhttps://journals.iucr.org/paper?S2059798325008356

A principled framework is provided for identifying the number of structurally meaningful classes in cryo-EM data sets #CryoEM #3DClassification #StructuralHeterogeneity doi.org/10.1107/S205...

A comprehensive validation process for helical symmetry parameters in the EMDB examines their consistency by combining high-throughput computational evaluation with manual verification #CryoEM #Validation #HelicalSymmetry doi.org/10.1107/S205...

October issue is now available! journals.iucr.org/d/issues/202... The cover highlights the article on categorizing prediction modes within low-pLDDT regions of AlphaFold2 structures from the Richardson lab @duke-university.bsky.social : doi.org/10.1107/S205... Image credit: Chistopher Williams

We’re thrilled to announce that abstract submissions for IUCr 2026 are officially OPEN 🎉 🗓 Deadline: February 15, 2026 📍 Location: Calgary, Canada 🔗 Submit your abstract today: www.iucr2026.org/ #IUCr2026 #CallForAbstracts #Crystallography #StructuralScience #science #IUCr

October issue is now available! journals.iucr.org/d/issues/202... The cover highlights the article on categorizing prediction modes within low-pLDDT regions of AlphaFold2 structures from the Richardson lab @duke-university.bsky.social : doi.org/10.1107/S205... Image credit: Chistopher Williams

🚨 Registration is open for the CCP4 Study Weekend 2026: “Ligands in Focus: Decoding Ligand Binding in Modern Structural Biology” 🎉 📅 7–9 January 2026 I’m honoured to be a scientific organiser with Tobias Krojer and Stephanie Wankowicz. 👉 Programme & registration: studyweekend.ccp4.ac.uk

I'm incredibly proud to present this @actacrystf.iucr.org interview with Alex Mendez and Cydni Bolling on their first paper ever! The interview was co-led by Dr Graham Chakafana from Hampton University in the USA, who supervised the authors' undergraduate research. www.youtube.com/watch?v=5rjI...

I've also purified ArnA by mistake (who hasn't?). Did mass spec before plunging it though, so never got this nice structure :)

S. Narasinga Rao and Manuel Soriano Garcia remember Rengachary Parthasarathy (1939-2025) doi.org/10.1107/S205...

40- and 71-substitution variants of hydroxynitrile lyase demonstrate the essential role of distant, indirectly acting residues to catalysis in α/β-hydrolase enzymes @UMNews #EngineeredProteins #HydroxynitrileLyases #HydrolaseFold doi.org/10.1107/S205...

The role of the hinge-loop composition in domain swapping in the c-Src SH3 domain has been studied by interchanging residues in the RT and n-Src loops between two SH3 domains #SH3Domains #DDomainSwapping #HingeLoop doi.org/10.1107/S205...

By integrating cutting-edge technologies and workflows, XFBLD-Platform addresses key challenges in fragment-based lead discovery, such as throughput, accuracy and data management #FragmentBasedLeadDiscovery #ProteinCrystallography #FragmentScreening doi.org/10.1107/S205...