Amazing piece from @science.org and @richardastone.bsky.social on our field and the growing connection between human and bacterial immunity! #microsky #virosky #immunosky www.science.org/content/arti...

1/8 🚨 New preprint from the @sternberglab.bsky.social & @martinjinek.bsky.social labs! CRISPR-associated transposases (CASTs) insert large DNA cargoes at precise genomic locations — no double-strand breaks needed.

Like a prophage being awakened from its slumber, the phage field has seen a lot of renewed activity during the last decade. Check out our new @natrevmicro.nature.com review on the temperate phage life cycle here to stay up-to-date 🧫🦠 www.nature.com/articles/s41...

Some phages are named after gods or rivers, this one was named by journalist Thilo Mischke @thilomischke.bsky.social after his grandma ("Oma Karin") while filming in our lab. It's a cool phage that should be temperate but became virulent due to a transposon at its lysogeny control! ⛓️‍💥💪🏼

Excited to see this online! The structure of the CAST type I holo-complex. Congrats to all involved! By the labs of @martinjinek.bsky.social & @sternberglab.bsky.social.

Exciting paper! - www.researchsquare.com/article/rs-8...

And another preprint about the Druantia defense system 👏🏻👏🏻 in case you were missing some reading for the weekend, here we go bsky.app/profile/lloe...

www.biorxiv.org/content/10.64898/2026.05.13.724313v1 www.biorxiv.org/content/10.64898/2026.05.12.722862v1 www.biorxiv.org/content/10.64898/2026.05.13.724758v1

Very happy to share our Druantia III–Zorya II synergy story, now on bioRxiv: www.biorxiv.org/cgi/content/short/2026.05.12.724681v1 It appears alongside several Druantia III papers from excellent colleagues, each telling a different part of the story. (links below)

Happy to share our latest pre-print on the Druantia defense system. We find that Type III Druantia uses a cooperative helicase-nuclease mechanism to sense and degrade replication-associated forked DNA structures. www.biorxiv.org/content/10.6... A🧵

When is an antiphage mechanism an evolved defence adaptation? This question has been on my mind for so long, because despite the field’s rapid progress there is currently no widely agreed framework in place to answer it. So @brownlab.bsky.social and I wrote this, and I'm happy to share the preprint!

For 40 years the 8-nt ‘Kozak Sequence’ was thought to mark sites of translation initiation. In a new study, we revise this model by identifying an ~80-nt sequence—the extended Translation Initiation Sequence (eTIS)— that guides ribosomes to correct start sites. 🧵 www.biorxiv.org/content/10.6...

1/9 New preprint from the Sternberg Lab in collaboration with the Nishimasu Lab! We uncover how the DRT3 antiphage immune system pairs two reverse transcriptases, one RNA-templated and one protein-templated, to build a double-stranded DNA effector. doi.org/10.64898/202...

Transposon end recognition and excision mechanisms of type I-F CRISPR-associated transposases www.biorxiv.org/content/10.64898/2026.05.05.722991v1 #cryoem

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Our lab is proud to present our latest work harnessing Bridge Recombinase for genome-scale editing in diverse bacteria, microbiome editing, and programmable horizontal gene transfer.

Excited to share our discovery of a new programmable RNA-guided DNA-targeting system hiding inside bacteriophages that predates CRISPR. We call it VIPR (Viral Interference Programmable Repeat), and it uses an entirely new logic to find its targets. Thread + link below.

Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering www.nature.com/articles/s41... #jcampubs

We found that TIR domains in animal immune proteins, including the human TLR4, are enzymes that cleave NAD+ to produce the signaling molecule cADPR Read our new preprint by talented Bohdana Hurieva: “Conserved catalytic activity of immune TIR domains in animals” www.biorxiv.org/content/10.6...

🎉Excited to share our new preprint! TIR domains from diverse animals, including human TLR4, are catalytically active and produce cyclic ADP-ribose (cADPR). This enzymatic activity is widespread in animals and conserved across the tree of life. www.biorxiv.org/content/10.6...

Structures of the QatBC complex from Angela Gao and Doug Wassarman in our group reveal an evolutionary link between QatABCD and Type IV CBASS and a role for protein deazguanylation in diverse anti-phage defense systems www.nature.com/articles/s41... www.science.org/doi/10.1126/...

Happy to share our new work on how phages escape bacterial immunity. We show that a phage homing endonuclease drives segmental amplification of anti-defense genes, pointing to a versatile and rapid mode of adaptation. www.nature.com/articles/s41... #PhageSky #MicroSky

Very proud of this work and all the efforts from my team and collaborators on this! You can now use DGRs for in vivo targeted hypermutagenesis in E. coli. We also included some early proof of concept in Yeast thanks to @seth-shipman.bsky.social !

I am so excited to share our new findings with you! We provide the structural evidence for a direct protein-to-DNA information pathway, showing how a bacterial enzyme 'reads' its own structure to 'write' DNA. www.science.org/doi/10.1126/...

Delighted to share our latest work on gene transfer agents (GTAs). We found a lysis control hub which allows GTAs to escape their bacterial host cells and transfer DNA 🧬 between bacteria. Thanks to @tunglejic.bsky.social, all co-authors, and our amazing collaborators! www.nature.com/articles/s41...

Diversity-generating retroelements for programmable targeted hypermutagenesis | Nature Biotechnology https://www.nature.com/articles/s41587-026-03078-4

Excited to share our new paper out today in @science.org 🎉 We show that HGT via natural competence drives diversification of chromosomal integrons in V. cholerae 🤩 Below a 🧵 on key findings incl. background on natural competence in V. cholerae 1/ #microsky #phagesky www.science.org/doi/10.1126/...

PostDoc position available! (links in post below) Feel free to share!

‼️Please share‼️ Nemudryi lab is looking for postdocs to join our team and study: 🦠 Bacterial phage defense mechanisms 🧬 RNA repair & CRISPR-based RNA editing Excited about RNA, host-virus interactions & mechanistic biology? Let's talk! Apply: explore.jobs.ufl.edu/en-us/job/53...

Bacteria have been fighting off viruses using a huge arsenal of molecular weaponry that scientists did not know about — until now. Researchers have identified proteins that could lead to virus-fighting drugs and technologies. go.nature.com/4dqQnXI

Excited to see our work out in Science today! Using machine learning to identify prokaryotic immune systems www.science.org/doi/10.1126/...

📣Huge preprint 🔔 Today we share something our group has been working toward for a long time, led by @lucasmoriniere.bsky.social We asked can we predict which receptor a phage targets from its genome sequence alone? For most phages, we couldn’t. So Lucas set out to do something I had only dreamed of.

How diverse is bacterial immunity ? We report in @science.org how language models allowed us to predict 2.4M antiphage proteins spanning >23K novel potential systems. 👏 @emordret.bsky.social, @alexhv.bsky.social & al doi.org/10.1126/scie... Explore them here defensefinder.mdmlab.fr/wiki/refseq_...

This paper started as an idea @albertomarina.bsky.social had many years ago… which of course means he was right all along 😄. Some of us just needed a few years (and a lot of experiments) to catch up. Grateful (and slightly humbled) to be part of this. Thanks Alberto! www.cell.com/cell/fulltex...

Open postdoc position in my lab on HGT and interbacterial competition. We seek candidates with a PhD in microbiology (or related fields)+ strong 1st-author publications. Curious, highly motivated, and dedicated team players ready to contribute fully are encouraged to apply. Details: tiny.cc/cz01101

The newly established NeCEN Utrecht is looking for a #cryoEM scientist! www.uu.nl/en/organisat... Work with a new Krios 5 + pFIB and support cutting-edge life science research. 📍 Utrecht | 🗓 Apply by 30 April Please share with your network!

Happy to share the final version of my postdoc work on bacterial CBASS immunity with @aaronwhiteley.bsky.social published in @cp-cellhostmicrobe.bsky.social www.cell.com/cell-host-mi...

Excited to share the first preprint from the lab! We show that ApeA defends against RNA phage infection by cleaving the phage genome: www.biorxiv.org/content/10.6...

Proper organelle regulation during mitosis is a must, but how do membrane-less condensates like PML bodies behave during cell division? In work led by Eric Aird, we found the balance of speckled proteins SP110 & SP100 to be the key. doi.org/10.1038/s415...

New preprint: Cyclic tri-adenylate signalling by a Panoptes anti-phage guard system with a CARF-TM effector. Great work led by Sabine Grüschow www.biorxiv.org/content/10.6...

Bacterial Schlafen proteins mediate phage defence (new work from @anemudraia.bsky.social and @artemnemudryi.bsky.social labs) www.nature.com/articles/s41... Schlafens are an ancient, mechanistically conserved family of tRNA-targeting immune effectors.

Out Now! Conditional activation of Cas13 enforces lysogeny in a native type VI-A CRISPR host #MicroSky

Out Now! Bacterial Schlafen proteins mediate phage defence #MicroSky

Excited to see this in print! Congratulations @artemnemudryi.bsky.social and @anemudraia.bsky.social!!

CBASS is a cyclic nucleotide-based antiviral system in bacteria that is related to cGAS-STING signaling in animals. One of the big questions is how CBASS is activated during phage infection? We made some progress on this during my final year in the Kranzusch lab. www.biorxiv.org/content/10.6...

Out today: We discovered new viral proteins that target immune signaling molecules, solely based on their AlphaFold-predicted shapes www.science.org/doi/10.1126/... Congrats Nitzan Tal and coauthors! Thank you Kranzusch lab for the fun collaboration! Linking below previous thread on our findings

@nitzantal.bsky.social @romihadary.bsky.social @soreklab.bsky.social use structure prediction and in silico binding site analysis to discover viral immune evasion proteins! Exciting for our lab @reneechang.bsky.social @riveralopz.bsky.social to help with this project. www.science.org/doi/10.1126/...

Out now! In collaboration with Leifu Chang, we uncover the molecular and structural underpinnings of CRISPR-Cas12f-like RNA-guided transcription systems! Links to the published articles: tinyurl.com/55kpavet tinyurl.com/sk6djwx3 Previous thread for the preprint: bsky.app/profile/did:...

Stunning new structural analysis and mechanism for Tmn anti-phage defense by @fnobrega.bsky.social www.biorxiv.org/content/10.6...

In our latest work, we characterise the Tmn defence system. We reveal plasmolysis as a new way to block phage infection, dramatically reducing secondary infections.