Improved cryo-EM reconstruction of sub-50 kDa complexes using 2D template matching www.biorxiv.org/content/10.1101/2025.09.11.675606v1 #cryoem

Happy for this to be out! We have documentation and tutorials here. Please give it a try and let us know what you think. lucaslab-berkeley.github.io/Leopard-EM/

Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules www.biorxiv.org/content/10.1101/2025.07.29.667395v1 #cryoem

@joshdcryoem.bsky.social will also be giving a talk about his prior work as a PhD student with Chris Russo developing strategies to take advantage of the reduced radiation damage at liquid Helium temperatures for cryo-EM. You can find their paper here: www.pnas.org/doi/abs/10.1...

If you are going to M&M, also see postdoc @joshdcryoem.bsky.social's poster about tilted vs untilted data collection for in situ structural biology www.sciencedirect.com/science/arti....

Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...

At the 2024 IMC meeting, Richard Henderson laid down a challenge. Design a #TEM single-particle #cryoEM lens with 1mm Cc at 100kV. I'm thrilled to reveal our new Arxiv preprint delivering a fully retrofittable pole-piece swap out for the @jeoleurope.bsky.social 1400 TEM. arxiv.org/abs/2505.11458

In the Bay Area? Love computational cryo-EM/ET? Come join us for the first edition of FrostByte - a meeting for the local computational cryo-EM methods community! Details and registration at frostbyte-cryoem.github.io Thanks to @joshdcryoem.bsky.social and Ariana Peck for help organizing!

For a shorter read, @mrclmb.bsky.social and @science.org also have articles about the helium story. www.science.org/content/arti...

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it. 🔗 www.pnas.org/doi/10.1073/...

Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1

In-Situ High-Resolution Cryo-EM Reconstructions from CEMOVIS https://www.biorxiv.org/content/10.1101/2025.03.29.646093v1

Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1

I've been wanting to see a paper using off-axis electron holography for low-dose biological imaging since the start of my PhD. Spatially resolved charge measurements👌

Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching ##3DReconstructionAndImageProcessing##SingleParticleCryoEM##Imaging@umassboston...##IUCrhttps://journals.iucr.org/paper?S2052252524011771

Laser Flash Melting Cryo-EM Samples to Overcome Preferred Orientation biorxiv.org/cgi/content/short/2024.11.21.624652v1