Austin E. Y. T. Lefebvre (@austin-lefebvre.bsky.social)

Analyzing organelles is HARD. They're complex, but critical to understanding biology. Current tools are manual, organelle-specific, or can't handle large 3D datasets. With an easy install and a click of a button, Nellie solves ALL these problems! nature.com/articles/s41... 🧵2/N

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Nellie: automated organelle segmentation, tracking and hierarchical feature extraction in 2D/3D live-cell microscopy - Nature Methods Nellie is a comprehensive automated pipeline for studying the structure and intracellular dynamics of diverse organelles that offers accurate segmentation, tracking and feature extraction on both 2D a... https://nature.com/articles/s41592-025-02612-7