This one is a bit of a departure from the usual and definitely a work in progress! We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below. Read on for details... 1/x

BIG NEWS!! We will be hiring a new professor with 25 years of endowed funding at the @biozentrum.unibas.ch @unibas.ch, focused on using biophysics and structural biology to explore climate-related fundamental science topics. Come start an innovative research cluster with us! 🧪🔬🧶🧬🌾🌍

+1 would sign - I used FlyBase *regularly* for my undergrad thesis on Drosophila genetics!

If you are thinking about the future of structural biology, we are too! Our two cents (Jürgen Plitzko and I) just got published online here: authors.elsevier.com/sd/article/S...

So awesome this is out in its final form. Congrats @jennysachweh.bsky.social , @becklab.bsky.social and team. Happy I could contribute a little to the story 😊

Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...

FlyBase, a Drosophila database, will lose a third of its team in early October because the Harvard grant that covered the employees’ salaries was canceled. Scientists warn that losing FlyBase could devastate fly research. By @claudia-lopez.bsky.social www.thetransmitter.org/community/ha...

Behind the paper article by Manel Llado Santaeularia on our manuscript on the in cell structure of a transposable element with @juliusbrennecke.bsky.social. Looking forward to exciting years @viennabiocenter.bsky.social Special thanks @sramundo.bsky.social for making this interaction possible!

Exactly two weeks left to submit your abstract to the EMBO Mobile Genome Workshop. ☝️this year we will select from abstracts 2 “long” talks to highlight last minute breakthrough from early career scientists. Apply! July 29th

Learn more about the collaboration possibilities offered at Vienna BioCenter as Victoria Deneke shares her insights as a Postdoc at the Research Institute of Molecular Pathology. The VIP³ PostDoc Program call is open – apply now! training.vbc.ac.at/post-docs/vi... #VBCPostDoc

We’ve uncovered Asgard chromatin structures formed by a Hodarchaeal histone : closed hypernucleosome conserved in archaea and an open form resembling the H3-H4 eukaryotic octasome. Fantastic work by @harshranawat.bsky.social! www.biorxiv.org/content/10.1... #Chromatin #Asgard #Archaea #cryoEM

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

Our work on HIV-1 matrix, led by James Stacey and @dhrebik.bsky.social, is in this week's @nature.com. Extended explanatory video from @margotriggi.bsky.social. www.nature.com/articles/s41...

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

1/ I am Jan-Michael Peters, Scientific Director of the Research Institute of Molecular Pathology (IMP) in #Vienna, part of the @viennabiocenter.bsky.social.

Super excited to share our latest work on deciphering the #Ubiquitin Code “𝗨𝗯𝗶𝗥𝗘𝗔𝗗 𝗱𝗲𝗰𝗶𝗽𝗵𝗲𝗿𝘀 𝗽𝗿𝗼𝘁𝗲𝗮𝘀𝗼𝗺𝗮𝗹 𝗱𝗲𝗴𝗿𝗮𝗱𝗮𝘁𝗶𝗼𝗻 𝗰𝗼𝗱𝗲 𝗼𝗳 𝗵𝗼𝗺𝗼𝘁𝘆𝗽𝗶𝗰 𝗮𝗻𝗱 𝗯𝗿𝗮𝗻𝗰𝗵𝗲𝗱 𝗞48 𝗮𝗻𝗱 𝗞63 𝘂𝗯𝗶𝗾𝘂𝗶𝘁𝗶𝗻 𝗰𝗵𝗮𝗶𝗻𝘀” @cp-molcell.bsky.social 1/8 www.cell.com/molecular-ce...

🤯 cryo-ET of copia viral-like particles lurking at the nuclear pore of intact Drosophila eggs! Amazing work by @svenklumpe.bsky.social & more cool findings in this collaborative paper illuminating the replication cycle of an iconic retrotransposon! www.cell.com/cell/fulltex...

Excited to share our latest work, led by the incredible @automnenine.bsky.social (now an Ass. Prof. at Princeton)! We uncover key insights into the retrotransposition mechanisms of LINE-1 ORF2p, the enzyme responsible for writing a significant portion of our genome. www.science.org/doi/10.1126/...

Great piece of work by our incoming joint PI @svenklumpe.bsky.social, already collaborating here with @imbavienna.bsky.social's Julius Brennecke and others. What a splash for a start!

Our #cryoEM technology group with scientists from @mpibp.bsky.social and @viennabiocenter.bsky.social, used #cryoET to capture retrotransposons in action, resolving copia in #fruitflies at sub-nanometer resolution inside cells. ❕Publication @cellpress.bsky.social: www.cell.com/cell/fulltex...

New paper alert! @svenklumpe.bsky.social, future Joint Fellow at IMBA and @impvienna.bsky.social, collaborating with the Brennecke Group at IMBA, used cryo-Electron Tomography to resolve the first structure of a retrotransposon inside the cell of a complex organism. Read more: imba.science/4itgs7L

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Our methods paper made it to bioRxiv: doi.org/10.1101/2025... Full #cryoET workflows for #plant tissues. Plus some general tricks and tweaks on serial Lift-Out and cryo-FLM. 🧪🧵1/5 #teamtomo #PlantScience #Physcomitrium #Arabidopsis #cryoEM #CLEM

Clearly heavy FSC discussion going on

🚨 Registration for the Electron Tomography and Subtomogram Averaging Workshop is now OPEN! 📝 Join us from March 24-28, 2025. 📍 Brazilian Center for Research in Energy and Materials (CNPEM), Campinas-SP, Brazil. 👉 pages.cnpem.br/cryoet-works...

Extremely excited to be joining the vibrant community of the Vienna BioCenter to build up cryo-ET and cryo-FIB milling on campus soon!

In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:

Happy to share our UBR4 structures. Check out the preprint to see how this giant E4 ligase complex selects substrates for ubiquitin chain extension. We also look at conservation and adaptations across humans, nematodes and plants. www.biorxiv.org/content/10.1... @clausenlab.bsky.social

🎄 xmas preprint 🎄 we are excited to share our cryo-EM structure of UBR4 in complex with KCMF1 and CALM1. the PQC ligase forms a massive ubiquitination arena, primed to amplify ubiquitin chains (E4 activity) and boost degradation of defective proteins. www.biorxiv.org/content/10.1...

this is now published y'all #giantvirus academic.oup.com/nar/advance-...

In Feb 2025 we will host a Virtual Wellcome - Advanced SPA Cryo-EM Course. The course will be for PhD student, postdoc, staff scientist or young group leaders. If you think it will benefit you go to the website for more info: astbury.leeds.ac.uk/facilities/w... Sharing will be appreciated :-)

My half-hearted attempt to start a structural virology starter pack. Could be useful for conference organisation in the future! 😀 go.bsky.app/Qxv95BL Comment below if you'd like me to add you, or anyone you recommend! #virology #cryoEM

Wohooo! Awesome to see this out. Still remember the spinach runs to the local market at Marienplatz you guys did haha. Congrats everyone!

Hello, Drosophila Community! We’re excited to join Bluesky and connect with researchers! At the Drosophila Genomics Resource Center (DGRC), we provide reagents to power your discoveries. Follow us for updates, tips, and discussions. What’s your favorite #Drosophila research question? Let’s chat!

Happy Fly Day! November 18th is #Drosophila day. It commemorates the isolation of a white-eyed mutation, which is now used across the world. That mutation is white[1118], with the numbers standing for Nov 18th, the day it was isolated by Bob Levis. 🪰

Happy #fly day! I learnt a year ago from @drosophilosophy.bsky.social that Nov 18th is #Drosophila day, as on that day the white[1118] mutant was isolated by Bob Levis. Here is an egg chamber (Sqh-mCherry= non-muscle type2 Myosin Drosophila, FBst0059024) imaged at cryo temperatures to celebrate :)

#NOTtheCover #ProtistsOnSky Unfortunately, we didn't make the cover @natureportfolio.bsky.social . I still think our proposition deserves sharing. Enjoy the read: www.nature.com/articles/s41...

Come work with us! Only a few days remaining to apply for the #cryoET / #CLEM facility scientist position at the @icrlondon.bsky.social! #cryoEM jobs.icr.ac.uk/vacancies/96...

Awesome to see so many people migrating towards bluer skies! Looking forward to the great discussions that will happen here (and have already happened)

Over 4 years in the making, I am extremely happy to share our pre-print on the “In-cell structure and snapshots of copia retrotransposons in intact tissue”. We used cryo-ET to resolve the copia capsid structure to subnanometer resolution inside cells: www.biorxiv.org/content/10.1...

This scientific meeting looks amazing!

Just a few days left to apply for our course @MPI_Biochem from 14. to 22. April 2024. If you want to learn about cryo-FIB milling, lift-out, and cryo-ET and have some fun along the way, apply by December 8th!

It is my pleasure to announce the @EMBO course on “In situ structural biology by Cryo-FIB and Cryo-ET” taking place @MPI_Biochem from 8.-14. April 2024. If you are interested in learning about cryo-ET and cryo-FIB including lift-out, apply by December 8th! meetings.embo.org/event/24-in-...

bioRxiv goes big on Bluesky: accounts for all subject categories now available (Please Reskeet) connect.biorxiv.org/news/2023/09...