New Article! Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion

New Article! Designing and testing CRISPRi-based synthetic gene circuits in plants

New Article! Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity

New Article! Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden

New Article! INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model

New Article! Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance

New Article! Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysis

New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET

Watch the webinar: "Methods Mythbusting: Insights from Journal Editors and protocols.io" www.youtube.com/watch?v=nbC9... This webinar was part of Love Methods Week 2026. excelscior.uc.pt/love-methods...

Review @natprot.nature.com @garybader.bsky.social Tutorial: annotation of animal genomes www.nature.com/articles/s41...

New Article! CellRank: consistent and data view agnostic fate mapping for single-cell genomics

New Article! Tutorial: annotation of animal genomes

New Article! Putting mammalian early embryonic cells into dormancy

New Article! Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices

New Article! Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives

New Article! Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas

New Article! Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water

New Article! Standardized protocols for analyzing lipocartilage and lipochondrocytes

New Article! A comprehensive framework for statistical testing of brain dynamics

New Article! GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis

New Article! Isolation, purification and characterization of lipocartilage in mice

Join us on 22 January 2026 for “Methods Mythbusting: Insights from Journal Editors and protocols.xn--io-02t protocols.io/webinars/met... at Love Methods Week 2026 excelscior.uc.pt/love-methods... #LoveMethods26

We’re thrilled to share our latest iTP-seq protocol for mapping bacterial translation landscapes in vitro — with a complete experimental workflow streamlined by Mélanie Gillard and an open source Python library for data analysis developed by Thibaud Renault.

New Article! Profiling active RNA polymerase II transcription start sites from total RNA by capped small RNA sequencing (csRNA-seq)

💡 Did you know? Methods are among the most cited research outputs. For #LoveMethods26, join experts for interactive sessions: 👉 Jan 21: @protocolsio.bsky.social Hackathon 👉 Jan 22: Methods Mythbusting Panel spklr.io/633288eAVM#LoveMethods26 #OpenScience

New Article! iTP-seq: a scalable profiling workflow to characterize bacterial translation landscapes in vitro

New Article! Upconversion particle-based optical tweezers for sensing applications

I edit manuscripts on very diverse topics and frequently have to look up words that are new to me. Today the word was "rugosity". A few weeks ago it was "psychrophilia". The question is: Can I assume that the interested reader will know what they mean?

New Article! Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq

New Article! Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level

New Article! Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring

New Article! Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles

New Article! In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology

Take a look at Nature Methods December issue for 8 methods to watch in 2026! Virtual cells, the cancer microbiome, protein editing, and more... www.nature.com/nmeth/volume...

Our Method of the Year 2025 is...drumroll please...EM-based connectomics!! Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬 www.nature.com/articles/s41...

Join Springer Nature editors and the protocols.io team for two exciting events at #LoveMethods26! 💻Transform your protocols at our Hackathon - Jan 21 🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22 👉FREE registration & more details: excelscior.uc.pt/love-methods...

New Article! CRISPR screens in human neural organoids and assembloids

#IgNobel face-to-face with Marc Abrahams (improbable.com/about/people...) presented at #FallingWalls #ScienceSummit25 features a favourite IgNobel winner, Kees Moeliker and his duck. www.youtube.com/watch?v=I1vP...

Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs at Love Methods Week 2026excelscior.uc.pt/love-methods...6/#LoveMethods2626

From Nature: 23 December 1869 - a news article about Mistletoe "When the leaves are rotting on the ground, and the fruit has been converted into cider..." www.nature.com/articles/001...

An essay on the history of the RI Christmas lectures published in nature last week. www.nature.com/articles/d41...

The Royal Institution of Science's Christmas lectures start today! www.rigb.org/christmas-le...

New Article! Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM)

New Article! Using tunable hydrogel microparticles to measure cellular forces

New Article! Bioengineering mini-colons for ex vivo colorectal cancer research

In another inspiring talk at #EMBOComp3D, Julia Mahamid spoke about recent in-cell cryo-ET results www.biorxiv.org/content/10.1...

New Article! Testing, quantification, in situ characterization and calculation simulation for electrocatalytic nitrate reduction

WEHI.TV explains discoveries at the frontier of medical research through accurate and entertaining 3D animation. www.wehi.edu.au/wehi-tv/ Drew Berry gave an inspiring talk at #EMBOComp3D

New Article! Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery

New Article! XomicsToModel: omics data integration and generation of thermodynamically consistent metabolic models