Nature Protocols
@natprot.nature.com
📤 4047
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đź“ť 226
New Article! CRISPR screens in human neural organoids and assembloids
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CRISPR screens in human neural organoids and assembloids
Nature Protocols, Published online: 19 December 2025; doi:10.1038/s41596-025-01299-6This protocol combines pooled CRISPR–Cas9 screening with neural organoid and assembloid models and illustrates how it can be applied to map hundreds of disease genes onto cellular pathways and specific aspects of human neural development.
https://bit.ly/3YEdd5e
6 days ago
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#IgNobel
face-to-face with Marc Abrahams (
improbable.com/about/people...
) presented at
#FallingWalls
#ScienceSummit25
features a favourite IgNobel winner, Kees Moeliker and his duck.
www.youtube.com/watch?v=I1vP...
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Ig Nobel Face-to-Face | Science Summit 2025
YouTube video by Falling Walls Foundation
https://www.youtube.com/watch?v=I1vPGUhQGSE
6 days ago
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Join us for “Methods Mythbusting: Insights from Journal Editors and
protocols.io”
protocols.io/webinars/met...
rs at Love Methods Week 2026
excelscior.uc.pt/love-methods...
6/
#LoveMethods26
26
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Methods Mythbusting: Insights from Journal Editors and protocols.io
This webinar will be held at: 3pm CET / 2pm GMT / 9am EST / 6am PST Join us for a lively session where we tackle common misconceptions about protocols and methods publishing. H...
https://protocols.io/webinars/methods-mythbusting-insights-from-journal-editors
11 days ago
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From Nature: 23 December 1869 - a news article about Mistletoe "When the leaves are rotting on the ground, and the fruit has been converted into cider..."
www.nature.com/articles/001...
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Mistletoe - Nature
Nature - Mistletoe
https://www.nature.com/articles/001214a0
14 days ago
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An essay on the history of the RI Christmas lectures published in nature last week.
www.nature.com/articles/d41...
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How the Royal Institution made science a seasonal spectacle
From flaming raisins to robots and rockets, the institution’s Christmas Lectures have educated and entertained for two centuries.
https://www.nature.com/articles/d41586-025-03976-8
15 days ago
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The Royal Institution of Science's Christmas lectures start today!
www.rigb.org/christmas-le...
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2025 CHRISTMAS LECTURES livestreaming
Find out where across the country you can watch a livestreaming of the 2025 CHRISTMAS LECTURES.
https://www.rigb.org/christmas-lectures/2025-christmas-lectures-livestreaming
15 days ago
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New Article! Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM)
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Visualizing infection by single positive-sense RNA viruses using virus infection real-time imaging (VIRIM)
Nature Protocols, Published online: 04 December 2025; doi:10.1038/s41596-025-01290-1Virus infection real-time imaging visualizes viral RNA translation within cells in real-time, revealing the earliest events of viral infection when viral protein and RNA levels are low, as well as the origins and consequences of cell-to-cell heterogeneity during virus infection.
https://bit.ly/3MhsUwp
21 days ago
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New Article! Using tunable hydrogel microparticles to measure cellular forces
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Using tunable hydrogel microparticles to measure cellular forces
Nature Protocols, Published online: 04 December 2025; doi:10.1038/s41596-025-01281-2Deformable poly-acrylamide co-acrylic acid microparticles (DAAM-particles) are tunable hydrogel microparticles for quantifying cellular forces. This comprehensive protocol details their synthesis, functionalization and applications.
https://bit.ly/49Z2pWn
21 days ago
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New Article! Bioengineering mini-colons for ex vivo colorectal cancer research
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Bioengineering mini-colons for ex vivo colorectal cancer research
Nature Protocols, Published online: 03 December 2025; doi:10.1038/s41596-025-01292-zThis protocol integrates state-of-the-art microfabrication, tissue engineering and optogenetic approaches to generate topobiologically complex miniature colons capable of undergoing tumorigenesis in vitro.
https://bit.ly/4pP6y3T
22 days ago
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In another inspiring talk at
#EMBOComp3D
, Julia Mahamid spoke about recent in-cell cryo-ET results
www.biorxiv.org/content/10.1...
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In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex
Cryo-electron tomography has emerged as powerful technology for in-cell structural biology, and in combination with breakthroughs in protein structure prediction, offers a unique opportunity for illum...
https://www.biorxiv.org/content/10.1101/2025.04.25.650208v1.abstract
22 days ago
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New Article! Testing, quantification, in situ characterization and calculation simulation for electrocatalytic nitrate reduction
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Testing, quantification, in situ characterization and calculation simulation for electrocatalytic nitrate reduction
Nature Protocols, Published online: 02 December 2025; doi:10.1038/s41596-025-01289-8Rigorous characterization is important for developing electrocatalytic reactions, for example, nitrate reduction. This workflow integrates bulk and surface techniques with in situ/operando spectroscopies, electrochemical measurements and density functional theory calculations.
https://bit.ly/49T8m7i
23 days ago
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WEHI.TV explains discoveries at the frontier of medical research through accurate and entertaining 3D animation.
www.wehi.edu.au/wehi-tv/
Drew Berry gave an inspiring talk at
#EMBOComp3D
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WEHI.TV Biomedical Animations | WEHI
WEHI.TV explains discoveries at the frontier of medical research through accurate and entertaining 3D animation.
https://www.wehi.edu.au/wehi-tv/
23 days ago
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New Article! Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery
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Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery
Nature Protocols, Published online: 01 December 2025; doi:10.1038/s41596-025-01291-0The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodology to perform the assay across a range of systems.
https://bit.ly/4pFVNR8
24 days ago
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New Article! XomicsToModel: omics data integration and generation of thermodynamically consistent metabolic models
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XomicsToModel: omics data integration and generation of thermodynamically consistent metabolic models
Nature Protocols, Published online: 01 December 2025; doi:10.1038/s41596-025-01288-9This Protocol describes XomicsToModel, a semi-automated pipeline that integrates bibliomic, transcriptomic, proteomic and metabolomic data with a generic genome-scale metabolic reconstruction or model.
https://bit.ly/48dXAr2
24 days ago
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New Article! Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies
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Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies
Nature Protocols, Published online: 28 November 2025; doi:10.1038/s41596-025-01277-yThe authors present a protocol for the use of two advanced DNA-based tension probes, the reversible shearing DNA-based tension probe (RSDTP) and ForceChrono probe, which provide powerful tools for studying mechanotransduction in living cells.
https://bit.ly/4rqMB4X
27 days ago
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reposted by
Nature Protocols
Communications Health
29 days ago
Welcome to Communications Health! We are a new, open-access journal from
@natureportfolio.nature.com
. Now open for submissions in public and global health!
www.nature.com/commshealth
#PublicHealth
#GlobalHealth
#OpenAccess
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Communications Health
Communications Health is an open access journal from Nature Portfolio publishing high-quality research, reviews and commentary in all population ...
https://www.nature.com/commshealth
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reposted by
Nature Protocols
Priya Silverstein
about 1 month ago
🌟 Love Methods Week 2026 🌟 📅 19–23 January 2026 | 💻 Online Join us for a full week of workshops & talks designed to boost the robustness, transparency, and reproducibility of your research methods!
#LoveMethods26
🔗 Learn more / Register 👇
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Love Methods Week 2026 – Excelsior
https://excelscior.uc.pt/love-methods-week-2026/
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reposted by
Nature Protocols
Rosy Favicchio
about 1 month ago
In this Protocol you will find comprehensive guidance for researchers, including those with limited electron microscopy experience, on the effective use of genetically encoded, multichannel EM-capsulin reporters for both fluorescence microscopy and EM workflows.
@natprot.nature.com
🧪 🔬
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Multiplexed genetic tags for electron and fluorescence microscopy - Nature Protocols
This protocol for the use and quality control of genetically encoded reporters to label proteins and cells in correlative light and electron microscopy workflows enables the acquisition of molecular, ...
https://www.nature.com/articles/s41596-025-01260-7
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Tools for evaluating methods are proliferating and it is difficult to choose which ones to use. This article also looks at how to improve their reproducibility. proper selection of greenness and related metric tools in analytical chemistry – a tutorial
www.sciencedirect.com/science/arti...
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Guidelines on the proper selection of greenness and related metric tools in analytical chemistry – a tutorial
The metric tools that aim to assess greenness, whiteness or related aspects of analytical procedures are gaining in popularity. At the same time, thei…
https://www.sciencedirect.com/science/article/pii/S0003267025004465?via%3Dihub
about 1 month ago
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This looks like a useful resource for choosing solvents. GreenSOL: Green solvent guide for analytical chemistry based on production-to-end-of-life assessment
www.sciencedirect.com/science/arti...
See also:
greensol.tuc.gr/greensol/
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GreenSOL: Green solvent guide for analytical chemistry based on production-to-end-of-life assessment
GreenSOL is the first comprehensive solvent selection guide tailored specifically to analytical chemistry. It employs a life cycle approach to evaluat…
https://www.sciencedirect.com/science/article/pii/S0165993625003991?via%3Dihub
about 1 month ago
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New Article! Directional conjugation of monoclonal antibodies to nanoparticles using metal-free click chemistry
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Directional conjugation of monoclonal antibodies to nanoparticles using metal-free click chemistry
Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01272-3This Protocol Extension describes directional conjugation of monoclonal antibodies to nanoparticles via the Fc antibody moiety using metal-free click chemistry, including procedures for synthesis, quality control and evaluation of nanoparticle–antibody conjugates.
https://bit.ly/43zkbM0
about 1 month ago
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New Article! Multiplexed genetic tags for electron and fluorescence microscopy
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Multiplexed genetic tags for electron and fluorescence microscopy
Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01260-7This protocol for the use and quality control of genetically encoded reporters to label proteins and cells in correlative light and electron microscopy workflows enables the acquisition of molecular, cellular and biochemical information at high resolution.
https://bit.ly/4phTKm6
about 1 month ago
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New Article! Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection
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Proteome-wide profiling of S-nitrosylated proteins using the SNOTRAP probe and mass spectrometry-based detection
Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01282-1This robust, proteome-wide approach for the exploration of the S-nitrosoproteome in human and mouse tissues uses the SNOTRAP probe and nano-liquid chromatography–tandem mass spectrometry analysis. The protocol enables efficient and high-throughput profiling of S-nitrosylated proteins in complex mixtures of biological material.
https://bit.ly/44lNLVp
about 1 month ago
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New Article! Multilevel characterization of genome editor nuclease activity with BreakTag
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Multilevel characterization of genome editor nuclease activity with BreakTag
Nature Protocols, Published online: 19 November 2025; doi:10.1038/s41596-025-01271-4BreakTag is a scalable next-generation sequencing-based method for the unbiased characterization of programmable nucleases and guide RNAs that allows off-target and nuclease activity assessment, as well as the characterization of scission profiles.
https://bit.ly/4picyBV
about 1 month ago
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New Article! Characterization of radiative cooling materials
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Characterization of radiative cooling materials
Nature Protocols, Published online: 18 November 2025; doi:10.1038/s41596-025-01273-2Reproducible characterization of materials designed for radiative cooling is important for their development and application. The protocol describes how to acquire optical data and how to build and use outdoor and indoor thermal testing platforms.
https://bit.ly/4pcjssk
about 1 month ago
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New Article! Extracting and characterizing microplastics and nanoplastics from environmental samples
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Extracting and characterizing microplastics and nanoplastics from environmental samples
Nature Protocols, Published online: 14 November 2025; doi:10.1038/s41596-025-01276-zThis Protocol describes preprocessing of different environmental samples and extraction of microplastics and nanoplastics, as well as qualitative and quantitative characterization of microplastics and nanoplastics. The analytical technologies used for characterization include attenuated total reflection-Fourier transform infrared spectroscopy, laser direct infrared spectroscopy and optical photothermal infrared microspectroscopy.
https://bit.ly/47IWyDa
about 1 month ago
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reposted by
Nature Protocols
Rosy Favicchio
about 1 month ago
Happy to be attending the Cellular therapy and immunotherapy conference 2025 in Hangzhou. I’m looking forward to learning about the latest developments in the field! 🧪
#medsky
mm.sciconf.cn/en/minisite/...
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Cellular Therapy and Immunotherapy Conference 2025
https://mm.sciconf.cn/en/minisite/index/29362
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The Nature Reviews department is recruiting for a locum editor with expertise in biological sciences. Location: Heidelberg or New York offices Application deadline: 26 November 2025 (the Nature Reviews department includes Nature Protocols)
springernature.wd3.myworkdayjobs.com/de-DE/Spring...
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Locum Associate or Senior Editor, Nature Reviews
Job Title: Locum Associate or Senior Editor, Nature Reviews Locations: Heidelberg or New York - hybrid working model Application Deadline: 26th November 2025 – candidates will be considered as they ap...
https://springernature.wd3.myworkdayjobs.com/de-DE/SpringerNatureCareers/job/Heidelberg/Locum-Associate-or-Senior-Editor--Nature-Reviews_JR104613
about 1 month ago
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New Article! Branching beyond bifunctional linkers: synthesis of macrocyclic and trivalent PROTACs
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Branching beyond bifunctional linkers: synthesis of macrocyclic and trivalent PROTACs
Nature Protocols, Published online: 11 November 2025; doi:10.1038/s41596-025-01283-0This protocol describes the synthesis of macrocyclic macroPROTAC1 and trivalent PROTAC SIM1, detailing the generation of the macrocyclic and trivalent cores and their conjugation to the respective ligands, for enhanced target protein degradation.
https://bit.ly/4qW58WE
about 1 month ago
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Editorial in Nature Climate Change: Making the most of the Methods Clear methods reporting is key for reliable + reproducible science + can also prevent an extended review process. We highlight Methods section requirements for a more efficient publication process.
www.nature.com/articles/s41...
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Making the most of the Methods - Nature Climate Change
Clear methods reporting is key for reliable and reproducible science and can also prevent an extended review process. We highlight Methods section requirements for a more efficient publication process...
https://www.nature.com/articles/s41558-025-02406-x
about 2 months ago
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reposted by
Nature Protocols
Communications AI & Computing
about 2 months ago
🚀 New journal alert! We are Communications AI and Computing: an open access space from
@natureportfolio.nature.com
for research in AI, ML, and computational science. Now open for submissions!
www.nature.com/commsaicomp/
#AI
#ML
#OpenAccess
#ComputationalScience
#NaturePortfolio
#Research
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Communications AI and Computing is open for submissions! You can submit your manuscript and/or find out about joining the editorial board.
www.nature.com/commsaicomp/
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Communications AI & Computing
Communications AI & Computing will be a broad journal covering applied computer science with a focus on the development of artificial intelligence methods and ...
https://www.nature.com/commsaicomp/
about 2 months ago
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New Article! Production of human blood-generating heart-forming organoids and sample preparation for advanced imaging
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Production of human blood-generating heart-forming organoids and sample preparation for advanced imaging
Nature Protocols, Published online: 30 October 2025; doi:10.1038/s41596-025-01268-zWe provide a protocol for generating human pluripotent stem cell-derived blood-generating heart-forming organoids, a multitissue model encompassing aspects of human cardiac, endothelial and hematopoietic co-development, and techniques to stain and clear these large samples for laser microscopy.
https://bit.ly/3WZGJlc
about 2 months ago
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NFDI4Chem develops and maintains a national research data infrastructure for chemistry research in Germany. There are links to repositories to share your datasets and other resources for data management.
www.nfdi4chem.de
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NFDI4Chem | Chemistry Consortium in the NFDI - NFDI4Chem
The NFDI4Chem supports chemists to collect, store, process, publish and re-use research data, so that their data meet the FAIR conditions.
https://www.nfdi4chem.de/
about 2 months ago
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New Article! Comparing methods for preparing slippery liquid-like polydimethylsiloxane coatings
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Comparing methods for preparing slippery liquid-like polydimethylsiloxane coatings
Nature Protocols, Published online: 28 October 2025; doi:10.1038/s41596-025-01253-6Slippery covalently attached liquid surfaces are useful in many applications that require low-static friction to droplets. This review compares six prominent methods for preparing polydimethylsiloxane bound to silica surfaces and highlights pitfalls and time savers.
https://bit.ly/4hsKBEI
about 2 months ago
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New Article! Navigating the data processing for cytometry-based single-cell proteomics
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Navigating the data processing for cytometry-based single-cell proteomics
Nature Protocols, Published online: 16 October 2025; doi:10.1038/s41596-025-01257-2ANPELA is a software package to compare and assess the performance of different workflows for processing single-cell proteomic data, ensuring the user selects the most appropriate processing workflow for their experimental design question.
https://bit.ly/4haTNxg
2 months ago
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New Article! Sonicated inks and focused-ultrasound writing enable deep-penetration acoustic volumetric printing
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Sonicated inks and focused-ultrasound writing enable deep-penetration acoustic volumetric printing
Nature Protocols, Published online: 15 October 2025; doi:10.1038/s41596-025-01258-1The preparation of multicomponent viscoelastic self-enhancing sono-inks, synthesized as phase-transition reversible acoustic absorbers, enables acoustic volumetric printing beneath diverse tissue types in optically scattering media.
https://bit.ly/3KP1Jbz
2 months ago
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New Article! Best practice mass photometry: a guide to optimal single-molecule mass measurement
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Best practice mass photometry: a guide to optimal single-molecule mass measurement
Nature Protocols, Published online: 13 October 2025; doi:10.1038/s41596-025-01255-4In mass photometry, the optical contrast generated by individual molecules at a glass–water interface enables mass-resolved quantification of biomolecular mixtures. This protocol describes how to optimize and validate this method.
https://bit.ly/4nPCJzJ
2 months ago
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A new protocol at BMC Methods: A novel Y-maze paradigm with enhanced sensitivity to subtle spatial recognition memory impairments in mice
bmcmethods.biomedcentral.com/articles/10....
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Client Challenge
https://bmcmethods.biomedcentral.com/articles/10.1186/s44330-025-00043-6
2 months ago
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reposted by
Nature Protocols
Rosy Favicchio
3 months ago
When discussing content proposals for
@natprot.nature.com
with potential authors I often end up covering the wide reach of our journal. Often researchers are interested in examples of articles which have seen wide uptake, accesses and use from the scientific community. Here, a🧵of readcube links: 🧪🎓
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Something to think about when talking to your favourite AI system: How to write effective prompts for large language models
www.nature.com/articles/s41...
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How to write effective prompts for large language models - Nature Human Behaviour
Effectively engaging with large language models is becoming increasingly vital as they proliferate across research landscapes. This Comment presents a practical guide for understanding their capabilities and limitations, along with strategies for crafting well-structured queries, to extract maximum utility from these artificial intelligence tools.
https://www.nature.com/articles/s41562-024-01847-2
2 months ago
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New Article! Manufacturing synthetic viscoelastic antigen-presenting cells for immunotherapy
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Manufacturing synthetic viscoelastic antigen-presenting cells for immunotherapy
Nature Protocols, Published online: 09 October 2025; doi:10.1038/s41596-025-01265-2Protocol for fabricating synthetic viscoelastic antigen-presenting cells and their application in T cell engineering. These synthetic cells support robust T cell activation and expansion and improve chimeric antigen receptor transduction efficiency.
https://bit.ly/3W079mt
3 months ago
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New Article! Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples
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Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples
Nature Protocols, Published online: 09 October 2025; doi:10.1038/s41596-025-01256-3Filter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combination enhances the resolution, throughput and reproducibility for data-independent acquisition-based mass spectrometry analysis.
https://bit.ly/46MCoaY
3 months ago
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New Article! Microfluidic gradients create a stem cell model of the human central nervous system
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Microfluidic gradients create a stem cell model of the human central nervous system
Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01269-yThe complex spatiotemporal signaling of human neural tube development is successfully engineered in a device using microfluidic gradients, creating a high-fidelity model of the developing human central nervous system.
https://bit.ly/470dEL5
3 months ago
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New Article! Investigating non-proliferative cell states with inducible CRISPR screens
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Investigating non-proliferative cell states with inducible CRISPR screens
Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01252-7CRISPR screens are widely used to identify essential genes and potential drug targets, but typical setups were designed for the study of highly proliferative cancer cell lines. Now, a protocol, using inducible Cas9, enables screens of non-proliferative cell states including senescence, quiescence and terminal differentiation, thereby expanding the applicability of CRISPR screens.
https://bit.ly/4773Yi6
3 months ago
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New Article! Inducible CRISPR–Cas9 screening platform to interrogate non-proliferative cellular states
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Inducible CRISPR–Cas9 screening platform to interrogate non-proliferative cellular states
Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01251-8Here, the authors provide a platform for inducible CRISPR–Cas9 screening to interrogate non-proliferative cellular states.
https://bit.ly/4mRCXor
3 months ago
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New Article! Generation of spatially patterned human neural tube-like structures using microfluidic gradient devices
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Generation of spatially patterned human neural tube-like structures using microfluidic gradient devices
Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01266-1Protocol for generating spatially patterned, human neural tube- and forebrain-like structures, by using a microfluidic device to impose orthogonal and independent chemical gradients on human pluripotent stem cells.
https://bit.ly/47mrWGf
3 months ago
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New Article! Measurement of electrochemical brain activity with fast-scan cyclic voltammetry during functional magnetic resonance imaging
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Measurement of electrochemical brain activity with fast-scan cyclic voltammetry during functional magnetic resonance imaging
Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01250-9The simultaneous recording of fast-scan cyclic voltammetry and functional magnetic resonance imaging signals enable the coupling of local tissue oxygen and neurotransmitter dynamics for measuring changes in the brain
https://bit.ly/48gNNRm
3 months ago
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Congratulations to all three winners! Susumu Kitagawa, Richard Robson and Omar M. Yaghi “for the development of metal-organic frameworks” We are honoured to have a Nature Protocol from Prof. Yaghi
rdcu.be/eJ0Es
www.nature.com/articles/s41...
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High-yield, green and scalable methods for producing MOF-303 for water harvesting from desert air
Nature Protocols - MOF-303 is a promising water-harvesting sorbent that can take up water at low relative humidity and release it under mild heating. This metal–organic framework can be made...
https://rdcu.be/eJ0Es
3 months ago
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reposted by
Nature Protocols
Nature
3 months ago
BREAKING: The Nobel Prize in Chemistry has been awarded to Susumu Kitagawa, Richard Robson and Omar M. Yaghi “for the development of metal-organic frameworks” Stay tuned for more.
#NobelPrize
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