I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

This movie shows lysosomes (orange) and keratin (gray) in a cultured cell over 10 minutes.

I like this movie, but a friend of mine likes to complain about the obvious stitching artifacts. I'll try harder next time, Michael. Vimentin (orange) and ER (blue) in an overnight acquisition.

What's up Bluesky....here's some live-cell imaging taken in my lab

is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end. Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social www.biorxiv.org/content/10.1...

Still posting cytoskeleton videos, it seems. Actin this time. Sample: Lifeact-eGFP in HeLa cells. Modality: Airyscan confocal Timestamp is mm:ss and the scale bar is 5 µm.

vimeo.com/1139537563?s... I uploaded a version of that vimentin IF movie to my vimeo page. It's still compressed, but looks a lot better. Best results when you set the quality to 4k.

Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.

iPS cell-derived cardiac myocytes (heart muscle cells) typically beat about once per second, so I usually speed up the movies I post; otherwise, scrollers might miss the action. But every now and then, a cell looks like this in real time. #CellBiology

www.nature.com/articles/s41... My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.

A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...

📕 Our Intermediate Filaments collection explores recent advances in intermediate filament research, focusing on lamins, keratins and vimentin. Explore new and recent JCB studies to accompany this week's European Intermediate Filament Meeting 👉 rupress.org/jcb/collecti... #Euro-IF

Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...

This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.

Intermediate filaments join microtubules in the self-repair club! Great work with in vitro vimentin filaments from @cecileleduc.bsky.social @romet-jegou-lab.bsky.social et al. in @pnas.org: doi.org/10.1073/pnas...

This is a cumulative maximum intensity projection movie of the endoplasmic reticulum labeled with the membrane marker mEmerald-Sec61B.

Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).

Is it just me or do these cells look a lot like the americas?

In the context of our @reviewcommons.org revision process, I'm happy to announce Microscopy Nodes v2.2.0! This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more! Preprint at doi.org/10.1101/2025...

📣 We’re thrilled to announce the judging panel for the 2025 #NikonSmallWorld photomicrography and Small World in Motion video competitions! 🔗 Click to learn about this year's judges: bit.ly/43hmGBq #Microscopy #Imaging #SciComm #SciArt

Actin sun.

FilaBuster - Vimentin IF fragmentation

In the classical view of mitotic cell division, the spindle apparatus maintains principal control of chromosome capture and alignment. Any breakdown in spindle function can result in chromosome mis-segregation, producing daughter cells with abnormal chromosome number.

I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)

Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells. www.biorxiv.org/content/10.1...

FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly https://www.biorxiv.org/content/10.1101/2025.04.20.649718v1

What started as a brief chat with @buvansr.bsky.social and @vgelfand.bsky.social at the 2023 CellBio meeting @ascbiology.bsky.social has grown into our published collaborative work on single vimentin IF dynamics and organization in cells. Love when stuff like that happens.

Challenging the view on #vimentin! our latest work shows VIF is an active, dynamic filament loosely held together rupress.org/jcb/article/... Thanks to @vgelfand.bsky.social @aaandmoore.bsky.social @nu-bsa.bsky.social @hhmijanelia.bsky.social @jcb.org #NUCDB #nikon #CrestOptics #livecellimaging

Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM rupress.org/jcb/article-...

Poor little vimentin stick man 😱 A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!

Vimentin (orange) and actin filaments (gray) in a COS-7. Airyscan images of the cytoskeleton always bring me joy. I square root transformed the vimentin so you can see the dimmer filaments out at the edge.

Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?

The N-SIM scope got its annual visit at @ncis-marseille.bsky.social and it's ready to deliver! Projected 3D-SIM stack of a COS cell labeled for actin ⚪ microtubules 🔵 and clathrin 🟠

Images from the ongoing MI-SIM demo for #FluorescenceFriday: COS cells labeled for actin, mitochondria or microtubules. The mosaic function is quite nice for 2D-SIM acquisition of large fields of view!

I have now also ported the color vision deficiency simulation app to Shiny live. Advantage is this runs entirely in your browser, even though you "upload" the image it never leaves your computer.

Can't wait to call my mom tomorrow and see if she knows what the clue "Making weaker, in gamer jargon" works out to... I think the nytimes puzzle is getting easier for Millenials and harder for baby boomers.

Our collaboration with the Germain Lab is now out in its final form!

We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇 www.biorxiv.org/content/10.1...

CD44 and Ezrin restrict EGF receptor mobility to generate a novel spatial arrangement of cytoskeletal signaling modules driving bleb-based migration https://www.biorxiv.org/content/10.1101/2024.12.31.630838v1

Stoked to share TWO preprints on mitochondrial pearling! 🎉 We uncover how spontaneous #mitochondria pearling drives #mtDNA nucleoid distribution (doi.org/10.1101/2024...), and @gavsturm.bsky.social et al. dissect the biophysics behind it (doi.org/10.1101/2024...). Details below! 🔬🧵🧪

The @addgene.bsky.social deposit is now live and can be found here - if you like self labelling enzymes and mammalian expression there’s probably some useful things in there for you 🎅 www.addgene.org/browse/artic...

This video is simply amazing.

I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.

🦠A team led by the Lippincott-Schwartz Lab used a new technique developed at Janelia called high-resolution template matching, or HRTM, to uncover in unprecedented detail the movement of ribosomes – the molecular structures that synthesize proteins inside cells. 🔗 hhmi.news/401LKMq

Here's a still image of the effect (hopefully less compressed)

Really bummed to have missed ASCB this year due to some last-minute circumstances, but excited that our manuscript describing an IF disassembly tool will (hopefully) be posted soon. Here's a movie of vimentin color-coded by orientation.

Vimentin-mSG FRAP

OpenOrganelle's macrophage data set: openorganelle.janelia.org/datasets/jrc... Loading OpenOrganelle data into neuVid: github.com/connectome-n...

OpenOrganelle is a repo of #CellBiology data, segmented from electron #microscopy at #HHMIJanelia by Aubrey Weigel and the CellMap team, the Saalfeld lab ( @herrsaalffeld.sky.social) and the Hess lab. Here's a macrophage cell, rendered with #Blender driven by neuVid (helpful for more than neurons).

Vimentin DNA-PAINT 🌞 in COS-7 cells #FluorescenceFriday