Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪

Check it out at PNAS today. Thank you Alexandre for the collaboration!! PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by Jing Ling, Yitong Liu, and all the collaborators! Available @spirochrome.com

Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social ! We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience tiny.cc/3qhp001

Hot off the press: The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience: rdcu.be/ek010

New from the lab: @maheandria.bsky.social and colleagues in collaboration with the Hiesinger, Stoeckli and von Kleist labs dissect the sequential emergence of axonal targeting during development in today's issue of Nature Neuroscience: rdcu.be/ek010

These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.

it is a filopodial (thin protusions) party in there! 🕺🧐 🧪 #liveimaging

This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging. I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉 🧪

Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!

A brief history of The Company of Biologists - 100 years of supporting scientists!👍👏 journals.biologists.com/jcs/article/...

Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology! To register go to biodesarrollo.unab.cl/mini-symposi... Come to enjoy a unique blend of science, sea, and food – all for free!

Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community. Read it below! blog.addgene.org/add...

2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)

How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling. www.biorxiv.org/content/10.1... Special thanks to @aliceyting.bsky.social for discussions

I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.

For this #FluorescenceFriday, let's watch an axonal growth cone (for a change) displaying its most beautiful filopodia (this time)! 🤩 They are so dynamic! 1 image taken every 500 ms for 5 min played at 15 fps. 🧪

I used VVD Viewer for this #FluorescenceFriday video: 141 volumes from the #HHMIJanelia FlyLight Split-GAL4 #Drosophila driver collection. The animation comes from a high-level description processed by neuVid. Such a rapid tour of the data has questionable scientific value, but it's fun to watch!

Vimentin-mSG FRAP

Lynn Landmesser was a giant in the field of developmental neurobiology. Sad to hear of her passing. #DevBio #neuroscience 🧪

This axonal growth cone from a culture of spinal neurons decided to display its most beautiful lamellipodia for this #FluorescenceFriday! 🤩 #neuroscience #liveimaging 🧪 1 image taken every second for 10 min, played at 15 images/s

Excellent preprint from @goodfrognosis.bsky.social’s lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social) www.biorxiv.org/content/10.1...

Come to be our colleague @pasteur.fr! center of Paris, great colleagues, outstanding facilities, permanent position, crème brûlée every Friday - what else could one want?

Viral tracing (yellow) in the mouse adult spinal cord (cyan, nuclei; magenta, axons). Image by the very talented Soizic Riche. #fluorescencefriday #fluorescentfriday

It’s time for another #FluorescenceFriday! 👩‍🎨🎨🔬🫀multi-color labeled zebrafish heart with mosaic expression of diverse myl7:color plasmids 🏳️‍🌈🌈

New @dev-journal.bsky.social editorial We know we have high standards - but we're committed to making publishing with us constructive From clear revision guidance to limitations sections & transparent peer review, we're here to help you share your science journals.biologists.com/dev/article/...

🚀 It's #FluorescenceFriday, and commissural axons are coming at you! 🧪🔬 15-hour time-lapse of chick commissural axons navigating and crossing the CNS midline (and reverse). One stack taken every 10 minutes. Pixels were color-coded in the z-axis. #LiveImaging #Neuroscience #Science #Microscopy 🧠✨

Successful transplant of hiPS-cell derived corneal epithelium in @thelancet.bsky.social

We are happy to report that our journals and community sites now have Bluesky accounts. Follow them at: @dev-journal.bsky.social @jcellsci.bsky.social @jexpbiol.bsky.social @dmmjournal.bsky.social @biologyopen.bsky.social @the-node.bsky.social @prelights.bsky.social @focalplane.bsky.social

Starting the week strong! Cheering myself up for the next challenges with 15 min of axonal growth cone dynamic madness. F-actin (cyan) and membrane (magenta) show their dance. One image taken every 10 s.🧠🔬 #MicroscopyMonday #LiveImaging #Neuroscience #Microscopy

I'm ashamed to say how long I've been staring at this video. 😅

Leave me alone it's #Fluorescencefriday !➡️ two axonal growth cones growing together until one got fed up of the other... #neuroscience #microscopy #liveimaging

I thought #fluorescencefriday would be a good opportunity for my first post here. Here are some crazy actin dynamics imaged with TIRF

Happy #FluorescenceFriday ! Let's celebrate with a colorful image of a developing ventral spinal cord of a 🐣 embryo. #neuroscience #microscopy

A little #neuralcrest for #FluorescenceFriday #DevBio

A 🦋-inspired neural tube for #FluorescenceFriday! Imaged by @siewzhuan.bsky.social #DevBio 🧪👩‍🔬

105 cool microscopists in this starter pack, but I know there are more out there! I want to find more 45 to complete the list, can you help me? 🤓🔬 go.bsky.app/Aaf8sKT

May commissural axons find their way in here 🙏 #Microscopy #LiveImaging #AxonGuidance #Neuroscience

Check out how #neuralcrest cells 🔬 migrate (animal= #zebrafish, white cells=sox10+, start=10 somites stage, anterior=top)

Hello Bluesky! I am an engineer/scientist currently working at the MRC LMB in Cambridge UK. During my PhD I developed new imaging technologies to shed light on the dynamics of living cells e.g. here is a movie of 6 organelles in live human cancer cells. Follow for more updates! #science #biology

A summary thread of our Cell Reports paper published last week. In brief, we found a novel activity for netrin1 in the developing spinal cord: it acts as a boundary, to limit Bmp signaling to the dorsal most spinal cord. 1/9 www.sciencedirect.com/science/arti...

Great starter pack-can we please be added? We ❤️ live imaging. #cilia #centrioles #microtubules #biosensors #LiveIsAlwaysBetter

My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks! go.bsky.app/KYYr7xc #liveimaging #livevellimaging #microscopy

My turn to create a starter pack for scientists using live imaging in their research! Of course, I’ve likely missed many—please let me know who else I should include. Thanks! go.bsky.app/KYYr7xc #liveimaging #livevellimaging #microscopy

We don’t study dividing cells, but I can respect what they’re trying to do. And always nice watching stress fibers blow up 💥🙏 @vucellimaging.bsky.social

Snapshot of commissural axons crossing the midline of the central nervous system #sciart #neuroscience (repost from the other side)

Let me know if you want me to add you!

Every time one of my manuscripts gets rejected, it ramps up... 🙊. I hate it! Very nice piece of advice!

Tunneling nanotubes enable intercellular transfer in zebrafish embryos pubmed.ncbi.nlm.nih.gov/39541978/