If you see this image and think "ha, fly testis!", you know your stuff. If you also know how transcription clusters form and disperse during sperm precursor formation, maybe don't read our new Advanced Science paper, out today 😉 advanced.onlinelibrary.wiley.com/doi/10.1002/...

✨Rare model organisms can teach us how to build a body differently. In snail embryos blastomeres can be as big as the rest of the embryo! 🐌Phrontis vibex blastula stained for tubulin (gray), phalloidin (blue) and histone (magenta). 🔬 #ModelMonday #DevBio 📸: Clemens Cabernard

A bit late, but plasmids for cloning-free PCR donors for endogenous gene Knock-In in fish are available on Addgene www.addgene.org/browse/artic...

Preprint Alert🚨🚨🚨 TPE FLIM of NAD(P)H is a promising readout for cellular metabolic changes. Wouldn’t it be great to multiplex it with fluorescent proteins marking other structures or specific cell types? YES! Unfortunately, this seems surprisingly hard 🤔😅: www.biorxiv.org/content/10.6...

What if light could help doctors see disease sooner? 🔬 This updated review explores advances in medical hyperspectral imaging, from improved sensors to AI‑powered analysis, showing how richer light data can support better diagnosis & surgical guidance. Read more in #SPIE_JBO: https://bit.ly/42ncbMO

2/🧵 SPARK (Simulation of Protein Accumulation from Reaction Kinetics) takes in experimentally-measurable binding site information and molecular kinetic parameters and generates molecular trajectories. It outputs simulated microscope images under user-defined imaging conditions (mag, exposure, etc.)

1/🧵 Can transcription factor condensate formation be explained without phase separation? Our new preprint introduces SPARK, a simulation tool that reproduces condensate behavior (clustering, fusion, FRAP) from diffusion & binding kinetics alone. Movie: 60 sec FRAP sim www.biorxiv.org/content/10.6...

🚀 Major Announcement I am honored to announce my new role as Director of the Center for Single Molecule Biophysics in ASU Biodesign Institute. biodesign.asu.edu/single-molec... #ArtificialIntelligence #BiomedicalResearch #Biophysics #PrecisionMedicine

🚀 KiloHz two photon live imaging #MultiphotonMicroscopy @laboptbio.bsky.social Two-photon light-sheet live imaging at kilohertz frame rate using birefringence-based pulse splitting #LightSheetMicroscopy #Bioimaging #Photonics #Microscopy #Zebrafish Article is available here: doi.org/10.1364/OPTI...

🔬 Happy to share our new paper showing that fluorophore brightness open a new dimension in SMLM microscopy ! Thanks to all authors @laurent-le.bsky.social S. K. Sreenivas @emmanuelfort.bsky.social ! @ismolab.bsky.social @instlangevin.bsky.social @cnrs.fr rdcu.be/fmIeD doi.org/10.1038/s415...

DySTrack is officially published!: doi.org/10.1242/jcs.... If anyone is keen on using it feel free to reach out as we are more than happy to help and demo if need be!

Our PEACH-PIT is alive! I grew tired of constantly missing the FOV in our experiments so why not automate the boring things. Big shoutout to my awesome senior design team for getting the first prototype. Now it’s time to finish the alignment and drop some bass (beads)

Preprint Alert🚨🚨🚨 TPE FLIM of NAD(P)H is a promising readout for cellular metabolic changes. Wouldn’t it be great to multiplex it with fluorescent proteins marking other structures or specific cell types? YES! Unfortunately, this seems surprisingly hard 🤔😅: www.biorxiv.org/content/10.6...

The peer-review system needs an update. Biology Open did a cool experiment - contract reviewers in advance, and compensate them for a job well done. The results: it accelerates the process without loss of quality! Very promising. It’s not like for-profit journals can’t afford it…

We hope you're pumped for next week's episode featuring Dr. Filipa Simões (@simoesfilipa.bsky.social) at @ox.ac.uk! Learn all about her research on how macrophages interact with the cardiac environment to enable heart regeneration. 💓 Check out her latest work: https://bit.ly/3RVoFJG

Directional elements: the preprints are out! 1/n Congratulations to our amazing team: biorxiv.org/content/10.6... biorxiv.org/content/10.6... biorxiv.org/content/10.6... We screened for principles governing global brain dynamics by developing a set of new methods: 1) conformal immersion microscopy;

Hello #world, meet 1,000× Expansion Microscopy. A small gel would grow to the size of an Olympic swimming pool, while amino-acid-scale distances become visible with ordinary light microscopy. Led by Helena Hu from @eboyden3.bsky.social's lab, in collab with us. Story: www.biorxiv.org/content/10.6...

LIPIDS!!! 🥰😎

Some serious #lipidtime in Dresden. Thanks to @lipid.bsky.social for organising the Bridging the Membrane meeting between Dresden and the UK Midlands. What an honour to be here and finally meet @nadlerlab.bsky.social Much fruit or rather lipid for thought 🧐🤔

What if polarization could carry more data? 🔦 A multilayer approach expands #polarization channels and reduces crosstalk, boosting information capacity for imaging, communication, and encryption. Read the full #AdvancedPhotonics article here: https://bit.ly/4lx294L

Live imaging of immune cell response (🟢) to Salmonella infection (🔵) in the hindbrain of a transgenic #zebrafish larva. Credit to Drs. Jade Leiba & Mai Nguyen-Chi. #ZebrafishZunday 🧪

SPLICECRAFT v1.0 IS LIVE!!!!!!!!!!!! Open your terminal and type in "pipx install splicecraft" if you want to try it out, then spam "splicecraft update" often as I push updates frequently. A labor of love for the community I adore. Enjoy! 💚

We're excited that plasmid for HYlight2, our improved sensor for fructose 1,6-bisphosphate, are now available on @addgene.bsky.social Pre-print here: www.biorxiv.org/content/10.6...

Happy #FluorescenceFriday. I found this embryonic macrophage contacting a non-myeloid cell (bottom right corner) and then moving away. What's cool is that this contact is strong enough to slightly bend the cell and you can just about to see it. Forces at the cellular scale is so curious! 😁

This #FluorescenceFriday banger is one of our first ever fish movies and a huge part of why I instantly fell in love with the model: a living embryo expressing GFP in its beating heart!!!!

The ZMBH welcomes Dr. James Sáenz @jamessaenz.bsky.social as new Professor in Molecular Biology. James previously led a research group at B CUBE - Center for Molecular Bioengineering of Dresden University of Technology.

OptiCell3D: Precise inference of mechanical cell properties from microscopy imaging https://www.biorxiv.org/content/10.64898/2026.05.23.727231v1

We enjoyed putting our heads together with the Jakob and Freddolino labs to find that polyphosphate modulates the formation of functional RNA-protein condensates in response to nitrogen-starvation stress! journals.plos.org/plosbiology/...

Excited to share this paper from @yiechanglin.bsky.social showing how lipids can move between bacterial membranes along the bridge like protein TamB. Also happy to be able to post the cool movie. 🎥🌟 #MDsimulations #StructuralBiology doi.org/10.1016/j.bp...

Light-based modulation of astrocytic calcium for regulation of organelle dynamics and #morphogenesis. New tool developed by Lan Yang, Mikael Björklund, Cong Yi, Shue Chen, and Shue Chen (Zhejiang University-University of Edinburgh Institute): rupress.org/jcb/article/... #neuroscience #astrocytes

Anisotropic subdiffusion in the cytoplasm of living cells https://www.biorxiv.org/content/10.64898/2026.05.21.726834v1

“We show that MS222 and quinaldine sulfate are the most suitable for achieving deep anesthesia, whereas etomidate is better suited for studies focused on the cardiovascular system. Only quinaldine sulfate was found to be aversive.” Very cool #3Rs work in #zebrafish 😎

🔬 Ever seen a mitochondrion “flicker”? We designed HBmito, a membrane voltage sensor, to catch nanoscale nanoscale electrical spikes in real time—the first optical glimpse of single mitochondrial channels in action. #Mitochondria #CellBiology #Biophysics link.springer.com/article/10.1...

Prof Jonathan Taylor of @uofgphysastro.bsky.social is leading a £4m new project with colleagues from @imperialcollegeldn.bsky.social and @sheffielduni.bsky.social to create detailed 3D images of the mechanical forces inside a living, beating heart. Read more here: www.gla.ac.uk/news/headlin...

High-dimensional data is hard to understand. But is it truly cursed? To help you build better intuition for high-dimensional datasets, @lekschas.de and I developed dtour, a visualization tool for smoothly navigating through data projections.

The more we learn about #glycotime, the more dramatic will be our revisions to textbooks on such basic principles as protein trafficking and organelle privileges www.nature.com/articles/s41...

Crazy news from Nottingham Uni. www.bbc.co.uk/news/article...

😃😀👏 Wooow All the membrane dynamics in one go Congratulations everyone 🎉👏 It's dynaMIET - quite literally.

📣There are more than 150 UK Institutions who are part of a deal between Wiley and Jisc which allows researchers free #OpenAccess publication in the Journal of Microscopy at no direct cost.

New preprint from my lab! Will Anderson developed a transgenic #zebrafish ubiquitously expressing an optogenetic FGF/ERK activator. We're having a lot of fun with it and think you will too! www.biorxiv.org/content/10.6...

New plasmids from Kai Johnsson now online! 💡 The self-labeling CLIP-tag2 achieves almost 1000-fold faster tagging than the original when combined with a new substrate optimized for live-cell imaging. https://twp.ai/4hrKYy

Very cool to see the power of de novo design applied to dye-labeling!

Meet MagLuc: a magnetically-controlled luminescent protein. This is a foundational tool for remote control of biological processes, also known as "magnetogenetics". Can you tell when the magnetic field turns on and off? From @mariaingaramo.bsky.social and the team at @nonfictionlabs.bsky.social

Very cool 😳 Extending the space of rotational diffusion measurements by exploiting triplet switching Very powerful! Congratulations.

We invented “triplet tumbling microscopy” (TTM) to track protein-protein interactions in living cells! Tag your protein with GFP and see it interact with *untagged* binding partners www.biorxiv.org/content/10.6...

#BlueSci question. Any recommendations for an (anti-rat) NIR 2y Ab to be used with a 730 nm laser line? The 2 obvious choices are AF750 or DyeLight755, with (on paper) AF750 brighter? Not sure about comparative photostability? No appropriate JaneliaFluors? @christlet.bsky.social any thoughts?

How can a single cell learn without a brain? We explore this in my paper with @wallaceucsf.bsky.social! We discovered that single cells may learn using molecules similar to those that animal brains use to learn, like CaMKII. Cells can also propagate memory states to their progeny!

First 22 hours of zebrafish development. Credit to @zebrafish007.bsky.social at #WeinsteinLab. #ZebrafishZunday 🧪

PinkyCaMP: a red genetically encoded calcium indicator with improved brightness and stability. @massecklab.bsky.social www.nature.com/articles/s41...

Leukocytes in a zebrafish larva moving towards a pipette filled with a chemoattractant (arachidonic acid). Credit to @niethammerlab.bsky.social @mskcancercenter.bsky.social. #ZebrafishZunday 🧪