have ppl encountered any IP problems getting to use any of the tools we've made? GCaMP? RCaMP, SnFRs, AAVretro, etc.? we tried to make access trivial and cheap. but if there are problems, please let me know. we could try to break patents on all those to make shareware versions, if this is a problem

hey team! I need advice! If there were theoretically an organization whose sole purpose was to systematically identify and break nuisance and stupid IP in the biomedical space, creating shareware equivalents, what would be the highest value targets? e.g., AAV serotypes, antibodies, CRISPR, 1/n

Nice! Thanks @lintianphd.bsky.social @loogerl.bsky.social @michaelzlin.bsky.social @rhodamine110.bsky.social Eric Schreiter + many other engineers for in vivo neuro. Also: Thanks to the virus and transgenic engineers, like @hongkuizeng.bsky.social Viviana Gradinaru, Josh Huang, etc. 1/2

Thanks to @nature.com for featuring our work in this piece on genetically-encoded sensors. The article does a great job highlighting their importance for both basic research and translational impact, such as in our lab's research on serotonin. www.nature.com/articles/d41...

On the latest ep of the Climate Biotech Podcast, hear how synthetic biology legend @loogerl.bsky.social is turning his protein engineering expertise to developing tools to study methane monooxygenase, an enzyme that could unlock methane removal. Listen in: podcasts.apple.com/us/podcast/t...

My absolute privilege to be interviewed by @homeworld.bsky.social‬: www.linkedin.com/feed/update/.... we talk methane, brains, tools, Alabama, you know - all the things. this is a fun and insightful podcast series. and HW is really getting it done in the granting/research space! check them out!

what do ppl think is going to happen to this 6 NIH grant app maximum? will it stick? how are people interpreting the whole co-PI vs MPI thing?

Thanks to Garden Grant co-funders @sparkclimate.bsky.social sky.social and @granthamcsf.bsky.social, and to Quadrature Climate Foundation for making this possible through programmatic and regranting support.

so proud of this team!!! they're changing the way grants are sought, written, and funded. and such critical work - greenhouse gases this cycle, focused on methane. they funded us (+ Michael Konopka, USNA) to study methane monooxygenase (pMMO), potentially the key to remediating methane pollution.

ok rumors were true all along: www.nature.com/articles/d41... steady slide into fascism. what are ppl hearing about US-based researchers with foreign citizenship? I am hearing rumors that they are being targeted now. Sounds laughable but all too real in this hellhole

GABA imaging classifies amacrine cell types in mouse retina! Aki and @keisukeyonehara.bsky.social are amazing! future goals include Gly imaging, 2-color Glu/GABA, more complete scRNA-seq/MERFISH mapped onto functional classes. Super-fun collaboration! @janeliagenie.bsky.social

hey, what are ppl hearing about NIH/NSF grants with international collaborators? A colleague has their NIH grant withheld bc it had intl collabs. are other ppl hearing this? any place to find info on this? thanks!

I think about this map a lot. www.bloomberg.com/graphics/201...

Critical ocean current AMOC weakest in 1,600 years and even without an imminent collapse its weakening will have a devastating impact on billions Prof Niklas Boers "in the short term, it doesn’t really matter if we have a strong weakening, say 80%, or a collapse.” www.theguardian.com/environment/...

Wondering if your study section cancelled? I update this sheet daily. As of today, 56/124 study sections that should have met since Jan 2, 2025 have "not met as scheduled." docs.google.com/spreadsheets...

does anyone have a gut feeling on when NIH/NSF grant reviews will resume? do ppl think they will? what are ppl doing - focusing on foundations and international opps right now? going into cost-saving mode? dos anyone have any advice for navigating this brave new world?

holy shit this seems real, posted by many ppl. It seems that @hhmi.bsky.social just killed its IE3 program. Does that kill Gilliam Fellows? lab member just applied. Freeman Hrabowski Scholars? is it all gone? I don't understand why @hhmi.bsky.social would be doing this; I look fwd to their statement

“unis will freeze all non personnel spending at 5pm today, place orders in ASAP." "Note that some university policies require orders to be delivered by the deadline to be covered, in which case a bulk order this afternoon might not do any good unless shipping is remarkably fast." god help us all

does anyone have any ideas how we're going to survive this? $ from state, companies, foundations? What can we do to support each other during this shit time? how can we fight back? it all feels so hopeless right now, I'm srsly looking into alternative careers.

Another epic announcement from the Homeworld team! Come look at Problem Statements, make a plan, and solve the world's most serious problem: greenhouse gas-driven warming and climate change. The grant process is easy, and creativity is encouraged! Spread the word!

have ppl found any funders that support toolmaking? I want to keep making tools for people, but there is a seemingly irresistible force pushing us towards hypothesis-driven research. In my experience, funders don't want to hear about tech dev. Any insights? for both neuro & climate would be great tx

hey who has big-picture knowledge about trees? specifically vaccinating them against various pathogens. what are regulations and economics on large-scale tree vaccination programs? for tree planting efforts, can saplings be easily vaccinated, vs. large adults that require deep injection? are GMO 1/

help please! anyone know good grantwriters to help with R01s, etc.? idea is start with 1 grant per person/entity to see how we work together, then long-term deals/employment options. open to any working arrangement. need help framing things in fundable fashion. will pay well for good stuff. thanks!

Tune into the latest episode of "Synaptic," and hear more about @lintianphd.bsky.social, the optimization of the GCaMP calcium indicator, and the lessons Tian's father taught her. Available wherever you stream podcasts. By Brady Huggett www.thetransmitter.org/synaptic/bio...

Excited to talk at the 1st Gene Therapy Initiative Symp at @ucsandiego.bsky.social next week! genetherapy.ucsd.edu/symposium-20... #gtiUCSD Will be talking about AAV engineering - 2.0 #AAVretro variants and other designed serotypes for basic research and human gene therapy!

This is that preliminary in vivo polyamine signaling data i was hinting at! we'll really tighten it up but there are pretty clearly some cool signals in the mouse brain

Paper alert! So happy to share the 1st paper entirely from our new home at UCSD Neurosciences- sensors for polyamines: spermidine, spermine, etc. What are those, you ask? (Autocorrect suggested "trampolines.") Polyamines (PAs) are ubiquitous: redox buffers, part. in endo/lysosomes.. 1/

pHW581 Depositor: Paul Sternberg Purpose: In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics www.addgene.org/198818/